Preoperative radiotherapy combined with chemotherapy and (or) targeted therapy not only reduces the local recurrence of locally advanced rectal cancer and increases the rate of anal preservation and pathologic complete response,but also improves patient compliance.Preoperative neoadjuvant therapy will gradually become the standard treatment for locally advanced rectal cancer.

Objective　To explore the changes and significance of the circulating immunocomplexes(CIC) in patients with chronic iridocyclitis.Methods　We assessed circulating immunocomplexes in peripheral blood of 45 patients with recurrent uveitis and 42 healthy controls by PEG deposition with the biochemical analyzer to obtain levels of CIC different with recurrent uveitis.Results　Levels of CIC were significantly higher in active patients(27.358±6.729)than those in controls(13.459±6.164,P＜0.001) and inactive patients (16.248±6.233,P＜0.001),but patients (inactive) had significantly higher levels than those in controls (P＜0.05).Conclusion　Levels of CIC were found in the groups of active patients with recurrent uveitis (27.358±6.729), but levels of CIC in inactive patients fell. The result of this study suggest that recurrence of uveitis is related to immunocomplexes,and changes in the levels of CIC could be also a measure standard of therapeutic effects.

Objective: To investigate the depression and anxiety in patients with asthma. Methods: With SAS and SDS,100 asthmatic patients were assessed. Results: The mean scores of SAS and SDS were 54.12±6.47, 44.8±7.28 respectively. 68% of sample had anxiety and 78% had depression. Conclusion: Most of patients with asthma have anxiety and depression. It suggests the treament of depression and anxiety for asthmatic patients are needed besides the conventional treatment.

This study was designed to evaluate the effect of estrogen to the growth of leiomyoma and the mechanism of testosterone propionate action on leiomy-oma.The levels of estrogen receptor in uterine tissud and those of estradiol and progesterone in uterine tissue and plasma were determined in 47 women with leiomyoma.The levels of estrogen receptor and estradiol in leiomyomatous tissues were 37.6?4.0 fmol/mg protein and 401.7?92.6 pg/g tissue,respectively.Both of them were higher than the corresponding levels in normal uterine tissues (P

Objective To discuss the expression and significance of eotaxin in the nasal secretions of the patients with nasal pol-yps .Methods The nasal secretion samples were collected from 40 patients including 15 cases of nasal polyps ,15 cases of chronic si-nusitis and 10 cases of nasal septum deviation .The enzyme-linked immunosorbent assay(ELISA) was adopted to detect the concen-tration of eotaxin .Results The average concentration of eotaxin was (468 .82 ± 440 .64)pg/mL in nasal polyps ,(443 .85 ± 334 .68) pg/mL in chronic sinusitis and (149 .23 ± 49 .01)pg/mL in nasal septum deviation .The eotaxin concentrations in the nasal polyps group and the chronic sinusitis group were higher than those in the nasal septum deviation group ,the difference was statistically significant(P<0 .05) .Conclusion The eotaxin concentration in the nasal secretions of the patients with nasal polyps is significantly increaseed ,which might be concluded that eotaxin may play an important role in the occurrence and development process of nasal polyps .

OBJECTIVE:To discuss the effect of Ethinylestradiol and cyproterone acetate tablet combined with metformin on ovulation induction of patients with polycystic ovarian syndrome(PCOS). METHODS:184 PCOS patients were randomly divided into control group(92 cases)and observation group(92 cases). Control group orally received 1 Ethinylestradiol and cyproterone ac-etate tablet in 5 d of menstruation,once a day,it stopped after 21 d,the next cycle of treatment was started on the 5th of with-drawal bleeding. Observation group additionally received 3 Metformin tablet,once a day,without drug withdrawal during menstrua-tion,and lasted for 3 menstrual cycles. After 3 menstrual cycles,all patients received ovulation induction treatment [(50-100 mg clomiphene was orally given from the 5th day of the 4th menstrual cycle,for 5 days,or 75-150 U postmenopausal gonadotropin by intramuscular injection,when the dominant follicle diameter was(19.0±2.0)mm,10 000 U human chorionic gonadotropin(HCG) was intramuscularly injected,the ovulation was continuously monitored and whether there was ovarian hyperstimulation syndrome (OHSS)was observed]. Body mass index(BMI),follicle estrogen(FSH),luteinizing hormone(LH),prolactin(PRL),estradiol (E2)and testosterone(T)before and after treatment,fasting plasma glucose(FPG),fasting insulin(FINS),insulin resistance in-dex(HOMA-IR),pregnancy rate,ovulation rate and the incidences of OHSS and adverse reactions in 2 groups were observed.RE-SULTS:Before treatment,there were no significant differences in the BMI,FSH,LH,PRL,E2,T,FPG,FINS and HOMA-IR between 2 groups(P>0.05). After treatment,BMI,LH,PRL,E2,T,FPG,FINS and HOMA-IR in observation group and LH, E2 and FINS in control group were significantly lower than before,and BMI,PRL,E2,T,FPG,FINS and HOMA-IR in observa-tion group were lower than control group,the differences were statistically significant(P<0.05). The pregnancy rate and ovulation rate in observation group was significantly higher than control group,the incidence of adverse reactions was significantly lower than control group,the differences were statistically significant (P<0.05). And there was no significant difference in the incidence of adverse reactions between 2 groups(P>0.05). CONCLUSIONS:Ethinylestradiol and cyproterone acetate combined with metfor-min can effectively improve insulin resistance of POCS patients,recovery sex hormone secretion,promote ovulation and improve pregnancy rate,with good safety.

Humans ; Intention ; Personnel Turnover

Objective: To make a comparative study on the differential diagnosis of the acute myeloid leukemia based on French-American-British Cooperative Group (FAB) and World Health Organization (WHO) standard. Methods: The 118 patients were recruited who were diagnosed with de novo AML. All cases were re-classified according to FAB and WHO classification standard. The median survival time and overall survival rate were recorded for prognosis evaluation. Results: Based on WHO classication standard, there were 35 AML patients (33.7%) with recurrent cytogenetic abnormalities, 21 patients (20.2%) with multilineage dysplasia, and 2 patients (1.9%) with therapy-related AML,and 46 patients (44.2%) AML not otherwise categorized. The 3-year survival rate were (75.1 ± 7.8)%, (47.4 ± 13.9) %, (50.0 ± 35.4)%, and (54.9 ± 10.0)% in different groups based on WHO standard. The difference was significant (P <0.001). Different karyotypes of chromosome had significant effects on the prognosis of AML. The median OS rate was (87.4 ±6.9)% for patients with good prognosis, (69.3 ±7.3)% for patients with moderate prognosis, and (19.6 ± 10.9)% for patients with poor prognosis. The difference was significant (P <0.001). Conclusion: Our study results suggest that classification of primary AML patients according to the WHO standard was more effective for evaluating progression and prognosis of AML.

Objective To explore the effect of c-Jun NH2-terminal kinase(JNK)signal transduction pathway on hyperoxia-induced lung injury in rats.Methods Twenty-four Wistar rats aged 3 weeks were randomly divided into 3 groups(n=8):room-air control group,7 d hyperoxia exposure group,and 7 d hyperoxia exposure with inhibitor of JNK intervention group.The rats in hyperoxia exposure group were exposed to high concentration of oxygen [fractional concentration of inspired oxygen(FiO2)≥950 mL?L-1] at normal pressure.The rats in room-air control group were placed in room air(FiO2=210 mL?L-1)at normal pressure.The rats in JNK inhibitor intervention group were intraperitoneally injected 30 mg?kg-1 SP600125 and exposed to hyperoxia 2 h later.The histopathological changes of lung tissues were observed by means of light microscope,therefore the changes of lung W/D weight ratio,total protein in bronchoalveolar lavage fluid(BALF)and lung permeation index were detected.The extent of lung cells apoptosis was analyzed by terminal deoxynucleotidyltrans-ferase-mediated dUTP nick end labeling assay.The protein level of p-JNK was measured by Western blotting analysis.Results Compared with room-air control group,conspicuous hyperaemia,edema,hemorrhage and extensive inflammation cells infiltration in the lung tissues were significantly observed in 7 d hyperoxia exposure group.The lung W/D weight ratio,total protein in BALF,lung permeation index,cell apoptotic index and the p-JNK protein levels of lung tissues all significantly increased in 7 d hyperoxia exposure group compared with those in room-air control group(Pa

Objective To study the optimum culture media and liquid conditions in shaking flasks for laccase production by Ganoderma lucidum. Methods Taking S_3 stain of G. lucidum as test materials and laccase activity of G. lucidum as measurement index to optimize the culture media and liquid conditions through orthogonal test. Results The optimum culture components of media were: Glucose 30 g/L, cotton 0.2%, (NH_4)_2HPO_4 0.66 g/L, casein 0.5%, Tween-80 0.15 mL; the optimum conditions were: initial pH value of medium was 5.5, 75 mL medium was in 250 mL-flask, inoculation was 12.5%, mycelium age was 5?24 h for 9?24 h culture. Conclusion Laccase activity of G. lucidum has been improved remarkably in the optimum culture media and liquid conditions.