ObjectiveTo observe the effect of the scalp acupunction on children with cerebral palsy.Methods56 cases with cerebral palsy were divided into two groups: the treating group(scalp acupuncture and modern rehabilitation) and the control group(modern rehabilitation only) with 28 cases each. The ADL and the motion function were compared. ResultsThere is a significant defference between the two groups on the ADL, while no significant defference was observed on motor function between the two groups.Conclusions Scalp acupuncture is effective on improvment of ADL of the children with cerebral palsy.

Aged ; Aged, 80 and over ; Anthracosis ; complications ; therapy ; Humans ; Male ; Middle Aged ; Pulmonary Heart Disease ; complications ; therapy

OBJECTIVE To investigate the cognition on hand hygiene and the reasons affecting the hand hygiene performance in hospital.METHODS Medical staff of 358 parsons were randomly selected by questionnaire.RESULTS It was higher cognition among medical staff on hand washing indication learned from Standards of Hand Hygiene among Medical Personnel in Medical Institutions(draft for soliciting opinions) issued by the Ministry of Health.The cognition coincidence of hand sterilization indication was 68.04%,but existed some less cognition or miscognition in this field among them.The main factors affecting the hand hygiene performance were busy at work,to which was the primary cause to affect use of stream water to wash hands.The concept that using stream water wash hand is reliable is the primary cause influence medical staffs to use alcohol scrape hand.CONCLUSIONS The nosocomial infection monitoring,guidance and control should be emphasized in the field of more use of the fast hand antiseptics instead washing hands and supervising the relevance of the amount of hand hygiene product consumpsion to nosocomial infections.

Alzheimers disease (AD) is a progressively and retrogressively neurodegenerative disorder of central nervous system severely threatening the aged. The studies of animal models, which share the very similar neuropathological characteristics with human, provide tools for the understanding of the etiology, mechanisms and medicine screening of AD. The distribution and chemical composition of amyloid-beta (A- beta) peptide -positive deposits were investigated in various non-human primate models. Various proportions of A- beta deposits contained cholinesterase activities, apolipoprotein E(ApoE) and alpha1-antichymotrypsin immunoreactivity. Alterations in the basal forebrain cholinergic system have been widely studied, but the magnitude of decline and relationship to cognitive impairment are still a matter of debate. This article also includes the latest research of the changing on tau, subtle structural alterations in axons, and neurofibrillary tangles (NFT) in the brain tissues of the non-human primate models.

Animals ; Breast Neoplasms ; diagnosis ; genetics ; DNA, Mitochondrial ; genetics ; Electron Transport Complex IV ; genetics ; Humans ; Loss of Heterozygosity ; Microsatellite Instability ; Mitochondrial Proteins ; genetics ; Mutation

ObjectiveTo probe into the maternal care utilization by minority women, for the purpose of policy recommendations on better maternal care in minority areas. MethodsA combination of stratified random sampling and typical sampling was made on 445 married women of reproductive age in six counties in Yunnan, Guizhou, Qinghai and Tibet provinces, a field survey on their utilization of maternal care services. ResultsTheir average prenatal detection rate is 78.24％, a level lower than the national rural average of 93.7％ and grade-4 rural average of 81.2％ in 2008; their post partook rate is 30.7％, lower than the national rural average of 54.3％ and grade-4 rural average of 58.9％ in the same period; their average coverage rate is 52.18％, a level lower than the national rural average of 87.1％and grade-4 rural average of 64.3％ in 2008. ConclusionThe maternal care utilization is found to be low for women in minority areas. Effective solutions are expected for payment of indirect expenditure of hospital delivery; better health education for enhancing health knowledge and health awareness of minority women; effective incentive mechanism for village doctors, consolidating the base of the three-level healthcare network.

BACKGROUND: The reaction of osteoblasts is important role in the reconstruction of osteocytes, and the mechanism of bone remodeling can be known from the cytological level through analyzing the mechanical reaction of osteoblasts under different loading.OBJECTIVE: To analyze the effects of low frequency vibration on the proliferation and differentiation as well as the matrix excretion of cultured human osteoblasts.DESIGN: A comparative observation.SETTING: Department of Orthopaedics, Nanfang Hospital of Southern Medical University.MATERIALS: The experiment was carried out in the laboratory of Nanfang Hospital from January to December in 2002. Human osteoblasts were isolated from the iliac cancellous bone of adults.METHODS: The osteoblasts were cultured for 48 hours, and then treated with low frequency vibration of 0.1, 0.2, 0.5, 2 and 5 Hz. The proliferation of the osteoblasts was detected with flow cytometry, and the activity of alkaline phosphatase (ALP) and content of osteocalcin were assayed by spectrophotometric methods and radioimmunoassay respectively.MAIN OUTCOME MEASURES: ① Effects of low frequency vibration on the ALP activity and osteocalcin excretion of osteoblasts; ② Effects of low frequency vibration on the proliferation of osteoblasts detected with flow cytometry.RESULTS: ① 0.2 and 0.5 Hz vibrations could markedly increase the ALP activity of osteoblast (P ＜ 0.01), but that of 5 Hz obviously decreased the ALP activity (P ＜ 0.01). 0.1 and 2 Hz vibrations had no obvious effects on the ALP activity of osteoblasts. ② 0.2 and 0.5 Hz vibration could significantly increase number of osteoblasts at S phase from 10.4% to 12.45% and 16.12%, and the proliferative index increased from 20.14% to 26.21% and 28.75%; 0.1 and 2 Hz vibration has not obvious effects in osteoblast proliferation index; 5 Hz vibration obviously decreased the proliferative index value to 13.22% (P ＜ 0.05). 0.1 and 2 Hz vibrations had no obvious effects on the proliferative index of osteoblasts (P ＞ 0.05). ③ 0.2 and 0.5 Hz vibrations could obviously increase the amount of osteocalcin excretion to 1.87 μg/L and 2.47 μg/L(P ＜ 0.05), but 2 and 5 Hz vibrations decrease the amount of osteocalcin excretion (P ＜ 0.05), and that of 0.1 Hz had no obvious effect on amount of osteocalcin excretion (P ＞ 0.05).CONCLUSION: Low frequency vibrations of 0.2-0.5 Hz could accelerate the proliferation and differentiation as well as the excretions of active materials of osteoblasts, and it plays an instructive role for low frequency vibration to treat fracture.

Pro-rich antimicrobial peptides are a group of linear peptides isolated from animals. These peptides have antibacterial activities and play an important role in innate immunity. Pro-rich antimicrobial peptides are devided into two classes:Pro-rich antimicrobial peptides from mammals and Pro-rich antimicrobial peptides from insects and other invertebrates.Members of Pro-rich antimicrobial peptides are all characterised by a high content of proline residues and predominantly active against Gram-negative bacterial species which they kill by a non-lytic mechanism,at variance with the majority of the known antimicrobial peptides. Evidence is accumulating that the Pro-rich peptides enter the cells without membrane lysis and,once in the cytoplasm,bind to,and inhibit the activity of DnaK protein that is essential to bacterial growth,thereby causing responsive bacteria death. This mode of action makes these peptides suitable for drug development efforts. As one of the best characterized Pro-rich peptides,PR-39 plays an important role in a number of biological processes. It has been found that PR-39 can induce the syndecan expression in mesenchymal cells, inhibit the NADPH oxidase activity of neutrophils and block the degradation of 1?B? and HIF-1?. These findings show that PR-39 can provide novel means in the control of inflammation, wound repair, ischemia-reperfusion injury,and angiogenesis for therapeutic purposes.

Objective To observe the analgesic effects of ethanol injection on advanced cancer patients by epidural two locus puncture. Methods Epidural two locus puncture was performed on 46 cases of advanced cancer patients. One locus was T 4 T 5, the other T 9 T 10 . Ethanol injection was used at the dose of 4 ml 95% ethanol on each locus. Results Satisfactory analgesic effect was found in all 46 patients. Conclusion Epidural two locus puncture is of wide diffusing range, low dose of injection and effective analgesia, which can overcome the disadvantage of partial analgesia by one locus puncture.

Mutations in presenilins caused most of the autosomal dominant familial forms of Alzheimers disease by altering ? secretase activity. The ? secretase is a large multiprotein complex including presenilin heterodimers,nicastrin,PEN 2 and additional unidentified components.? secretase cleaves beta amyloid precursor protein,Notch,E cadherin,ErbB 4 receptor tyrosine kinase and other membrane proteins.The development of various ? secretase inhibitors not only provides a tool for investigating the structure, function and mechanism of ? secretase,but also presents a therapeutic strategy to slow progression of Alzheimers disease pathology. Threre are various classes of ? secretase inhibitors: compounds containing a difluoro ketone or a difluoro alcohol group, (hydroxyethy) urea peptidomimetics,compounds possessing a hydroxyethylene dipeptide isostere, short helical peptides, non peptidic inhibitors derived from 4 chloro isocoumarin synthon, compounds containing alanyl moiety.Among these species of ? secretase inhibitors,those which selectively affect Abeta production are especially the most promising drugs to treat Alzheimers disease.