Bioreactors ; Humans ; Liver Failure, Acute ; therapy ; Liver, Artificial ; classification ; Sorption Detoxification ; instrumentation ; methods

Objective To study whether neuronal injury induced by platelet activating factor (PAF) was involved in NMDA /PSD 93 signaling pathway.Methods Primary cortex neurons culture were from wild type and PSD 93 knockout mice.Then the neurons were pretreated with 0.3 ?mol/L PAF for 24 hours, 5 ?mol/L BN52021, 10 ?mol/L MK-801, or 60 ?mol/L L-NAMA, respectively. The cells were stained with PI/Calcein for apoptosis detection. Varied protein expressions were also observed in the neurons by western blot. Proteins colocalized on neuraxon were detected by cell immunochemistry and confocol microscopy, and cGMP activity was measured by radioimmunoassay.Results (1) PSD 95, NR2A, and nNos except PSD 93 expressed in the cortex neurons from PSD 93 knockout mice. (2) PSD 93, NR2A and nNos colocalized on neurite. (3) Neurotoxicity and cGMP avtivity induced by PAF decreased in PSD 93 knockout cortex neurons.Conclusion NMDA/PSD 93 signaling pathway is involved in neuronal injury induced by PAF.

Objective To study the effect of millimeter wave radiation on human hepatoma cell. Methods BEL7404 hepatoma cells were cultured in vitro and randomly divided into 4 groups: the control group, the group radiated by millimeter wave for 30 min, the group treated with Fluorouracil(5 FU), and the group radiated by millimeter wave and treated with 5 FU simultaneously at same time. The ability of 35.8 GHz millimeter wave to induce the apoptosis of hepatoma cell was evaluated by analyses of fluorescence microscopy, DNA fragmentation assay and flow cytometry assay. Results BEL7404 cells radiated by the millimeter wave had the typical characteristics of apoptosis. Comparaed with the control group [(3.21? 1.06)%], the apoptosis rates were higher in 30 min radiating or/and 5 FU groups[ (14.33? 2.66)%, (18.58? 2.57)%, (27.91? 3.66)%]. Poly adp ribose polymerase(PARP) was found to be cleavaged in all the cells in millmeter wave radiation or/and 5 FU groups. Conclusion Radiation of 35.8 GHz could induce apoptosis of BEL7404 cell in vitro, and could act synergistcally with 5 FU treatment.

Objective To study the expression of proliferating cell nuclear antigen (PCNA), CDK4 and P16 on rat hepatocellular carcinoma by millimeter wave radiation. Methods Fourty male Wistar rats were randomly divided into four groups. Group one to three were feeded by diethylnitrosamine (DEN). Group one was a tumor control group. In group two and three the liver was directly radiated by 35.8 GHz, 100 mW/cm 2 millimeter wave for 20 min, twice a week for 10 or 5 weeks. Group four was a normal control radiated group. Fourteen weeks later all rats were sacrificed to undergo serological test and immunohistochemical stain of liver. Results The serum levels of ? glutamyltransferase in group two and three were lower than that in group one. Adenocarcinoma was only existed in group one by histological examination of liver tissue. Other groups of DEN exposure only had basophilic and eosinophilic nodules. Liver tissue expression of PCNA and CDK4 in group two and three were significantly lower than in group one, but the expression of P16 in group two and three was higher than that in group one. Conclusions Radiation with millimeter wave can partially inhibit cell proliferation and suppress the DEN induced hepatocellular carcinoma in rats.

Objective The practical evaluation of Rosco Disk Diffusion method on the clinical antifungal susceptibility test for yeast-like fungi. Methods The Fluconazole susceptibility test was detected by Rosco Disk Diffusion method and NCCLS M27-A broth macrodilution method with 76 yeast-like fungus strains isolated from clinical specimens. Three standard strains were used as the quality control. Results Coincidence rate of the two methods was 90.8%,The sensitive strains detected by one method didn't show resistance in another detection method, and the resistant strains detected by one method yet didn't show sensivity in another.Conclusion Rosco disk diffusion method can be used in clinical detection instead of NCCLS M27-A broth macrodilution method.

Animals ; Animals, Newborn ; Hypothyroidism ; genetics ; Myocardium ; metabolism ; RNA, Messenger ; genetics ; Rats ; Rats, Wistar ; Receptors, Thyroid Hormone ; genetics

Adenoma, Sweat Gland ; pathology ; Humans ; Male ; Middle Aged ; Nose Neoplasms ; pathology

Objective To discusses the effect of different nursing methods on anastomotic healing after low colorectal surgery. Methods 60 patient with colorectal cancer undergoing low anastomosis were randomly divided into the experimental group and the control group with 30 cases in each.The experimental group adopted modified nursing care,while the control group received routine nursing care.The defecation times and incidence of complications were observed between the two groups.Results The defecation frequency of the experimental group was less than the control group,and the incidence of rectal stimulation sign,anastomotic fistula and obstruction was less in the experimental group.Conclusions Modifed nursing methods have certain effects on preventing postoperative complications of low colorectal anastomosis.

Objective To explore the effects of Compound Malt Pill on gene expressions of androgen receptor (AR) and insulin receptor (INSR) in polycystic ovarian syndrome (PCOS) rats; To discuss its relevant mechanism of action.Methods Letrozole was used to induce and establish rat models. Rats were randomly divided into normal group, model group, Diane-35 group, low-, medium- and high-dose Compound Malt Pill groups. The normal group and model group received normal saline by gavage; the Diane-35 group was given Diane-35 by gavage; Compound Malt Pill groups were respectively given different concentrations by gavage for 21 d. The levels of serum testosterone (T) and insulin were measured by ELISA, and the gene expressions of AR and INSR were measured by RT-PCR. Results Compared with normal group, the serum T, insulin and the gene expressions of AR and INSR in model group increased significantly (P<0.05,P<0.01); compared with model group, the serum T, insulin and the gene expressions of AR and INSR in low-, medium- and high-dose Compound Malt Pill groups decreased significantly (P<0.05, P<0.01).Conclusion Compound Malt Pill can adjust endocrine-metabolic disorder in PCOS rats.

Objective To evaluate effects of simultaneous inhibition of mammalian target of rapamycin complex 1(mTORC1)kinase and glycogen synthase kinase-3β(GSK-3β)on phosphorylation of 4E-binding protein-1(4EBP1), cap-dependent translation, as well as survival and apoptosis of melanoma cells. Methods Cultured A375 cells were classified into several groups to be treated with dimethyl sulfoxide (DMSO group), the mTORC1 kinase inhibitor everolimus at a concentration of 5 nmol/L (everolimus group), the GSK-3β kinase inhibitor AR-A014418 at a concentration of 10 μmol/L (AR-A014418 group), or 5 nmol/L everolimus and 10 μmol/L AR-A014418(combined treatment group). After additional culture, Western-blot analysis was performed to measure protein expressions of phosphorylated 4EBP1 (p4EBP1)and survivin in A375 cells, m7GTP pull down assay to estimate interaction between eukaryotic initiation factor-4E (eIF4E)and eIF4G, cell counting kit 8 (CCK8)assay to evaluate cell proliferation, and flow cytometry to detect cell apoptosis. Results Both everolimus and AR-A014418 had inhibitory effects on 4EBP1 phosphorylation and survivin expression. The expressions of p4EBP1-65 and survivin were both significantly decreased in the everolimus group (0.74 ± 0.05 and 0.71 ± 0.06 respectively), AR-A014418 group (0.62 ± 0.06 and 0.58 ± 0.07 respectively)and combined treatment group (0.14 ± 0.04 and 0.09 ± 0.05 respectively)compared with the DMSO group (1.00 ± 0.07 and 1.00 ± 0.06, respectively, all P < 0.001), with the most significant decrease observed in the combined treatment group. As m7GTP pull-down assay showed, the everolimus group, AR-A014418 group and combined treatment group all showed significantly lower relative expression levels of eIF4G(0.72 ± 0.04, 0.67 ± 0.05 and 0.12 ± 0.05 vs. 1.00 ± 0.06, all P < 0.001), but significantly higher relative expression levels of 4EBP1 (1.98 ± 0.16, 2.32 ± 0.17 and 7.58 ± 0.25 vs. 1.00 ± 0.08, all P < 0.001)than the DMSO group, and the combined treatment group showed the lowest eIF4G expression but highest 4EBP1 expression. After 24-hour culture, the proliferation of A375 cells was inhibited by 18.5% ± 1.3% in the everolimus group, 19.8% ± 1.8% in the AR-A014418 group, and 61.2% ± 2.1% in the combined treatment group compared with the DMSO group, with the strongest inhibition noted in the combined treatment group. The inhibitory effects of everolimus and AR-A014418 on cell proliferation increased over time, and showed the same trend at 48 hours. Flow cytometry showed that the apoptosis of A375 cells was accelerated by the 24-hour treatments with everolimus and AR-A014418 alone or in combination, with the apoptosis rate being 14.28% ± 2.18%, 14.57% ± 2.35% and 55.18% ± 6.27% in the everolimus group, AR-A014418 group and combined treatment group respectively, and the combined treatment showed the strongest accelerating effect. Conclusion The combined treatment with everolimus and AR-A014418 can evidently inhibit 4EBP1 phosphorylation and eIF4F complex formation in A375 cells, which then suppress cap-dependent translation and promote apoptosis of melanoma cells.