The authors report a case of Von hippel Lindau disease in a 20 years old patients with presenting signs: diminution of visual acuity, intracramial hypertension with a left cerebollar syndrome and existence of an retinal angioma on the right side and papilla oedema on the left side. A scanning examination revealed a cystic tumor with a solid little mass. Angiography showed a capillary nodus coressponding to the mass lesion, with feeding arteries coming from the anterio superior cerebellar artery. Removal of the cyst and the tumor was successful. Pathological examination: Hemangi oblastoma. Negative ultrasound examination of the kidney, pancreas.
Hippel-Lindau Disease ; Case Reports [Publication Type]

Anal Canal ; surgery ; Humans ; Postoperative Complications ; Rectal Neoplasms ; surgery

Objective To investigate the sensitivities and specificities of enzyme-linked inununosorbent assay(ELISA)and immunogold labeling technique in the detection of anti-human immunodeficiency virus antibody.Methods(1)Sensitivity test:15 sera from patients with acquired immunodeficiency syndrome(AIDS)diagnosed by western blotting were diluted proportionately,we detected the anti-human immunodeficiency virus antibody in all diluted sera using ELISA and immunogold labeling technique,and recorded the maximum dilutions of the two methods that the anti-human immunodeficiency virus antibody was positive.(2)Specificity test:200 sera from patients with nonAIDS were detected by ELISA and immunogold labeling technique,and we recorded the negative rates of the two methods.Results(1)Sensitivity test:The maximum dilution of ELISA was significantly higher than that of immunogold labeling technique(P ＜ 0.05).(2)Specificity test:The negative rates of the two methods were 100％,and there was no significant difference between the two methods.Conclusion The sensitivity of ELISA in the detection of antihuman immunodeficiency virus antibody is better than that of immunogold labeling technique,but the sensitivities and specificities of the two methods are consistent when the serum is detected,so the immunogold labeling technique is able to replace ELISA as a screening test for AIDS.

Objective To investigate the clinical effectiveness of peripherally inserted central catheters(PICC) and peripheral intravenous catheters(PIV) in tumor chemotherapy. Methods A total of 180 patients with intravenous chemotherapy were randomly divided into two groups. 108 patients in the PICC group, 72 patients in the PIV group. The holding time and complications were observed and compared. Results The rate of complication in the PICC group were much lower than the PIV group, however was much longer than the PIV group in holding time. But there was no statistical difference in the positive culture rate of bacteria of two groups. Conclusions It is very safe to use PICC which can reduce the rate of complication of catheters and venipuncture times and is worthy of spreading in practice.

Parenteral nutrition (PN) is one of the important measures to cure premature babies and critically ill infants.In recent years,with the improvement of rescue techniques in the perinatal period,a growing number of preterm infants alive,very low birth weight and low birth weight infants gradually become an important part of the neonatal disease.The gastrointestinal development of these premature babies are not mature or combined with other diseases,in a long period of time after birth need partial or complete PN support.Due to the use of PN,provides the children with survival and greatly reduce the case fatality rate,but the cause of parenteral nutrition associated cholestasis would become one of the main complications of premature infants,seriously affect the patient's quality of life.In this paper,the treatment of premature parenteral nutrition associated cholestasis status is summarized.

ObjectiveTo discuss the influence of health education to the pregnant women and their relatives on patients' satisfaction degree in grass-root hospitals. Methods200 pregnant women in our hospsital were divided into the experimental group and the control group, the experimental group was given systematic health education, while the control group only received the traditional reception. Then a survey was carried out in the two groups and the satisfaction degree of maternal women and their relatives with medical workers was compared. ResultsThe satisfaction degree Of maternal women and their relatives in the experimental group was higher than that of the control group. ConclusionsHealth education in gy-necology and obstetrics department and carrying out nursing with humanity can improve satisfaction degree of maternal women and their relatives to medical staffs.

Objective:To observe the therapeutic effects of treating traumatic gonarthromeningitis with acupuncture combined Chinese herbs.Methods:Seventy Patients were randomly divided into two groups,with 35 cases in each group.The treatment group was treated with Chinese herbal medicine,acupuncture and cupping therapy.The control group was treated with Diclofenac Sodium Tablets,Corydalis Analgesic Tablets and Vitamin C Tablets.Results:The total effective rate of the treatment group was higher than that of the control group.There was a statistically significant difference between the two groups(P＜0.05).Conclusion:The combined therapy with acupuncture and Chinese herbal medicine has a remarkable therapeutic efrect on treating traumatic gonarthromeningitis.

As a connecting bridge between innate immunity and specific immunity,the Toll-like receptors (TLRs) play a significant role in inflammation,cellular signal transduction,and cell apoptosis.TLRs are also essential factors participating in immune regulation and immune escape in the pathogenesis and development of malignant tumors.A large number of clinical and experimental research have shown that the abnormal expression of TLRs can be detected in adenocarcinoma,such as lung cancer,breast cancer and colon caner.In recent years,the relationship between gynecologic malignant tumor and TLRs has received great interest.This article will review and summarize research progress of TLRs in ovarian cancer,cervical cancer,and endometrial neoplasia.

Objective To investigate the infection and distribution situations of diarrheogenic Escherichia coli among food practi‐tioners in Changsha City and to understand the carrying status of its virulence genes .Methods Totally 258 food practitioners from 9 units of Changsha City were extracted as the research subjects .Diarrheogenic Escherichia coli was detected by adopting the real time fluorescence multiple PCR .Results The infection rate of diarrheogenic Escherichia coli was 15 .5% (40/258) ,in which EAEC accounted for 10 .9% (28/258) ,EPEC for 3 .1% (8/258) and EHEC for 1 .6% (4/258) respectively ,EIEC and ETEC were not found .Among 28 strains of EAEC ,26 strains carried uidA gene ,13 strains carried aggR+pic gene and 27 strains carried astA gene , the carrying rates were 92 .9% ,46 .4% and 96 .4% respectively ;eight strains of EPEC all carried eae and uidA genes ;among 4 strains of EHEC ,3 strains carried eae gene ,moreover all 4 strains carried stx1+ stx2 gene .Conclusion Establishing a mechanism for continuously monitoring diarrheogenic Escherichia coli and deeply studying its molecular epidemiologic theory not only provide the essential data for the supervisory departments formulating and evaluating the public health measures ,but also has an important significance for evaluating the food safety status ,which can effectively control infectious source and protect the people′s health .

The adoptation of PCR-Sequence specific primers (SSP) for HLA-DQB"medium resolution" typing was reported. This method is rapid,easy,acourete and can be used for DQB genetic typing, is useful tool for matching of organ transplantation.