Objectives: To evaluate the analgesic effect of electronic dental anesthesia(EDA).　Methods: Sixty patients with dental caries in need of cavity preparation and 15 patients with pulpitis in need of pulp treatment were anesthetized with EDA, another 60 patients with dental caries in need of cavity preparation and 15 patients with pulpitis in need of pulp treatment were anesthetized with traditional local anesthesia(LA).　 Results: For patients with dental caries in need of cavity preparation, the effective rates in EDA group and LA group were 93.33% and 98.33% respectively(P>0.05).For patients with pulpitis in need of pulp treatment ,the effective rates in EDA group and LA group were 26.7% and 93.33% respectively(P<0.01).　Conclusions: EDA can be an effective analgesic method for cavity preparation, but not for pulp treatment.

Objective To investigate the effect of acupuncture at points Taiyang and Fengchi on the expression of heat shock protein 70 (HSP70) and neural cell apoptosis during local cerebral ischemia and reperfusion in rabbits and explore its protective action on nerve cells and the mechanism.Methods Rabbits were randomized into sham operation, model and acupuncture groups. Each group was randomly allocated to three time points: one, three and seven days. A rabbit model of local cerebral ischemia and reperfusion was made by middle cerebral artery occlusion (MCAO)using an intraluminal thread. Reperfusion was performed at two hours after cerebral ischemia. In the acupuncture groups, points Taiyang and Fengchi were electro-acupunctured at six hours after cerebral ischemia. Treatment was given once daily. The rabbits were sacrificed to take materials at one, three and seven days after reperfusion. Pathological changes in brain tissue were observed by HE staining. Cerebral HSP70 expression was determined by immunohistochemical method. Neural cell apoptosis was examined by TdT-mediated dUTP nick end labeling (TUNEL).Results Pathological staining showed that cerebral infarction area decreased significantly, the number of nerve cells increased and inflammatory infiltration reduced at every time point in the acupuncture group of rabbits compared with the model group. The results of histochemical examination showed that HSP70 expression decreased in the sham operation group and increased in the model group. It increased significantly at every corresponding time point in the acupuncture group compared with the model group (P<0.01). The number of apoptotic cells increased gradually with reperfusion prolongation and reached the peak at three hours in the model group. The number of apoptotic cells decreased significantly at every time point in the acupuncture group and there was a statistically significant difference by comparison with the model group (P<0.01).Conclusions Electroacupuncture at points Taiyang and Fengchi can increase cerebral HSP70 expression and decrease neural cell apoptosis to produce a nerve-protecting effect in rabbits with cerebral ischemia-reperfusion injury.

Mitochondrial aldehyde dehydrogenase(ALDH2),one of the isoforms of aldehyde dehydrogenase,has multiple enzymatic functions including the activity of dehydrogenase and esterase.The metabolisms of ethanol,amino acids,biogenic amine,vitamin or steroid in the body produce various substances of aldehyde.With the help of co-factor NAD(P)+,ALDH2 can convert aldehydes into corresponding carboxylic acid,which plays a key role in reducing toxic effects of aldehydes on the body.It does not need co-factor when ALDH2 works as esterase.It can convert carboxylic ester or other acids into corresponding carboxylic acids or alcohols.Recently,it has been shown that the decrease of ALDH2 activity exacerbates multiple factors(such as ethanol,ischemia)-induced myocardial injury and accelerates the development of nitroglycerin tolerance.Therefore,the development of specific agonists of ALDH2 may provide a novel approach to the therapy and prevention of heart diseases.

Fundamental specialized courses are important due to their connection between fundamental knowledge and major knowledge,which are regarded as the difficult courses for undergraduates to learn.The useful approaches and experience of bilingual teaching in microbiology,one of the fundamental specialized courses for the students majoring in Bioengineering,are presented here.The key aspects such as choosing textbooks,teaching manners and teaching contents are also discussed.This paper also proposes how to improve the quality of bilingual teaching during the fundamental specialized courses.

Objective To evaluate the feasibility and influence factors of ultrasound guided thrombin injection(UGTI) for the treatment of iatrogenic femoral pseudoaneurysms(PA). Methods Fifteen iatrogenic PA patients following femoral arterial puncture for coronary arterial angiography and PCI were treated with UGTI. One coronary arterial angiography and 14 PCI were performed in 5 males and 10 females. Nine simple PA with one lobe and 6 complex PA with two or three lobes. PA form,size,neck length and width,thrombin dose, therapic effect and complications were analysed. Duplex sonographic follow-up examination was performed at 24 hours and 5-7 days. Results Mean volume of PA was (13?5)cm 3. Twenty-one thrombin injections were performed. Mean thrombin dose was (250?120) IU in simple and (650?150) IU in complex PA. Primary success rate was 100% for 9 simple and 66.7 % for 4 of 6 complex PA. Recurrence occurred in one complex PA after 24 hours of UGTI and another complex PA reappear was detected after 15 days of UGTI, PA were completely obliterated after 3 times thrombin injections. Secondary success rate was 100%. No thromboembolic,infectious,allergic complications occurred. Conclusions UGTI is safe and effective as first-line therapy of iatrogenic pseudoaneurysms following femoral arterial puncture for PCI. Geometry form of PA is determinant factor of primary success rate.

Adult ; Cryptococcosis ; physiopathology ; Cryptococcus neoformans ; Duodenal Diseases ; microbiology ; physiopathology ; Humans ; Male

Objective To investigate the diagnosis value of laparosco-pie examination in ascites.Methods The data of twenty-three cases with ascites of unknown origin undergoing laparescopie examination and pathologic examination were retrospectively analyzed from May 2004 to August 2008.Results Twenty-two eases were definitely diagnosed by laparoscopie exploration,the positive rate was 95.6%.One ease re-mained unknown.Among them,18 eases were tuberculous peritonitis; 3 eases were metastatic adenocarcino-ma; one ease was peritoneal mesothelioma.No obvious postoperative complications occurred.Conclusion Laparoscopie examination is a safe method with hige definite diagnostic rate,which has important values in diagnosis of ascites of unknown origin.

Objective To investigate the chronic renal failure (CRF) patients with serum hyaluronic acid (HA),procollagen type Ⅲ (PC-Ⅲ),collagen type Ⅳ (C-Ⅳ) and laminin (LN) joint detection of clinical significance.Methods Using chemiluminescence method in 80 cases of CRF patients (CRF group) and 60 healthy (control group) serum HA,PC-Ⅲ,C-Ⅳ and LN for joint detection and comparison.Results The serum HA,PC-Ⅲ,C-Ⅳ in CRF group was significantly higher than that in control group [(305.37 ± 41.28) μ g/L vs.(73.26 ± 21.21) μ g/L,(236.39 ± 56.63) μ g/L vs.(68.46 ± 32.55) μtg/L,(112.54 ±26.73) μg/L vs.(55.36 ± 18.44) μg/L] (P ＜0.01).The LN there was no statistically significant difference between two groups (P ＞ 0.05).Conclusion Detecting HA,PC-Ⅲ,C-Ⅳ for the early diagnosis and therapeutic efficacy observation of patients with CRF has important clinical significance.

Objective:To investigate effects of strengthening the spleen and tonifying the kidney and their combination before and after exercise on relative indexes of metabolism of glucose and amino acids.Methods:Strengthening the spleen alone,tonifying the kidney alone or their combination before and after exercise was given to the rat receiving 7-week gradually increasing load exercise on a running platform,and after quantitative load,contents of serum lactic acid,urea nitrogen,glucose,hepatic glycogen,muscle glycogen,hemoglobin,and malondialdehyde(MDA)in renal tissue and activity of superoxide dismulase(SOD)in the renal tissue were determined.Results:Strengthening the spleen before exercise increased hepatic and muscle glycogen contents,and tonifying the kidney before exercise increased muscle glycogen content,and strengthening the spleen combined with tonifying the kidney before and after exercise increased hemoglobin level,strengthening the spleen followed by tonifying the kidney before exercise decreased serum lactic acid and urea nitrogen levels,increased muscle glycogen content and SOD activity/MDA content ratio in the kidney.Conclusion:Strengthening the spleen and tonifying the kidney play a certain role in reducing motion-related fatigue,while strengthening the spleen followed by tonifying the kidney before exercise has the best results.

Objective To investigate the morphological characteristics of vertebral-basilar artery in patients with posterior circulation ischemia vertigo. Methods The data of cerebrovascular disease related risk factors,neck CT angiography,and head MR angiography of 122 patients with posterior circulation ischemia vertigo and 60 non-vertigo volunteers with cerebrovascular disease related risk factors over the same period were analyzed retrospectively. The positive index of single factor comparison was analyzed by using multivariate logistic regression analysis. The risk factors for posterior circulation ischemia vertigo were screened,and the degree of vertebral artery stenosis and the degree of basilar artery tortuosity of the posterior circulation ischemia group were compared with those of the control group. Results (1 )The incidences of hypertension,hyperlipidemia,previous history of stroke or transient ischemic attack,vertebral artery atherosclemtic stenosis,vertebral artery variation,and basilar artery tortuosity of the posterior circulation ischemia group were 59%(n=72),55. 7%(n=68),64. 8%(n=79),41. 0%(n=50),28. 7%(n=35),and 30. 3%(n=37),respectively,and they were all significant higher than 33. 3%(n=20),31. 7%(n=19),35. 0%(n=21),15. 0%(n=9),10. 0%(n=6),and 15. 0%(n=9)of the control group. There were significant differences (all P<0. 05). (2)Vertebral artery atherosclerotic stenosis (OR,3. 891, 95%CI 1. 721-8. 800,P <0. 01),vertebral artery variation (OR,3. 231,95%CI 1. 238-8. 432,P =0.017),and basilar artery tortuosity (OR,2. 664,95%CI 1. 189-5. 972,P=0. 017)were the independent risk factors for posterior circulation ischemic vertigo. The degrees of vertebral artery stenosis and basilar artery tortuosity of the posterior circulation ischemic group were higher than those of the control group. There were significant differences (all P<0. 05). There was significant difference in the vertebral artery dominance between the degree of basilar artery tortuosity ≥grade 2 and