Objective To investigate the effect of Platelet-activating factor(PAF)in pathogenesy of asthma in high altitude Methods ELISA and other methods were used to detect the quantity of platelet(PLT)and the level of serum platelet-activating factor(PAF)in the plain control group、the plateau control group and the plateau asthma patients group.Results The quantity of PLT in plain control group and plateau control group were significantly lower than that of plateau asthma patients group.(P

In this paper the investigation of using a new method-ABC-ELISA in assay of Autoimmune Thyroid Disease are presented. The sensitivity of ABC-ELISA is compared with that of standard ELISA; Its reliability is proven by the methods of detecting TSAb with FRTL-5. TRAb is detected by ABC-ELISA in 91% of untreated Graves'. TRAb is detected by Standard ELISA in 70% of untreated Graves'. The results of ABC-ELISA in 26 untreated Graves' are equal to that of the method of detecting TSAb with FRTL-5. Therefore, we consider that ABC-ELISA is a sensitive, reproducible, convenient method applicable to clinical practice.

Objective: To investigate the protective effect of adenosine on hearing in acute acoustic trauma and its modulatory action in the cochlea. Methods:Guinea pigs were exposed to 118 dB SPL white noise for 30 min. During the process of exposure, artificial perilymph or artificial perilymph containing 1 mmol/L adenosine was infused into the cochlea through perilymphatic perfusion. Thresholds of compound action potentials (CAP) evoked by click and tone burst were measured before and after noise exposure. Results: Immediately after exposure, the mean threshold shifts evoked by click of the control group and adenosine treated group were (40?6.68) dB and (20?5.70) dB respectively. The threshold shifts evoked by tone burst at 2 8 kHz in the control animals were 32 50 dB, while in the adenosine treated animals, 20 35 dB. There were significant differences between the 2 groups except that at 6 kHz. Two hours after exposure, there was an increase of 4 8 dB at all frequencies in the control group; no changes in the adenosine treated group were found. There were significant differences in threshold shifts between the 2 groups at all test frequencies. Conclusion: Adenosine has protective effect on temporary threshold shift in acute acoustic trauma in guinea pigs. [

OBJECTIVE:To probe into the status quo and requirements of drug utilization in Haiqin Sanatorium Area of Nanjing Military Region Hangzhou Sanatorium.METHODS:The drug utilization in 710 convalescents in Sanatorium Area from May 2007 to April 2008 was analyzed statistically.RESULTS:A total of 10 categories of drugs were used in the 710 convalescents,chiefly including anti-cold drugs,antihypertensive drugs,drugs for external use,antimicrobial agents,ophthalmic drugs,otorhinolaryngological drugs,vitamins and gastrointestinal drugs.The rate of drug use conforming diagnosis was 87.32%.Oral administration occupied 71.13%.CONCLUSION:The drug utilization in convalescents in Haiqin Sanatorium Area is basically rational.However,there are also some unreasonable points in the drug utilization which remain to be tackled further.

Objective To determine the minimum alveolar concentration(MAC) of sevoflurane without body movement during laryngeal mask airway(LMA)intubation in premature infants less than 37 weeks of corrected gestational age undergoing total inhalation general anesthesia induction.Methods Twenty-one ASA Ⅰ or Ⅱ premature infants less than 37 weeks of corrected gestational age undergoing elective inhalation general anesthesia were enrolled in this study.At first,the general anesthesia induction was started by inhaling 6 ％ sevoflurane.After the premature infant lost consciousness,the end tidal sevoflurane concentration(ET-sev)was adjusted to the predetermined concentration and maintained stable for 15 min.After that,LMA was inserted.The up-anddown sequential allocation was used to determine MAC.The initial ETsev was 2 ％,which was increased or decreased by 1 gradient concentration in the next case according to the LMA insertion body movement response.The adjacent concentration gradient was 0.2％.The midpoint from th body movement response to non-body movement response was set as the balance point and the mean value of the concentrations of sevoflurane at all the balance points were calculated as MAC.Results The end tidal sevoflurane con centration without the body movement responses to LMA insertion was 1.71％.Conclusion The MAC of sevoflurane without the body movement responses to LMA insertion in premature infants less than 37 weeks of corrected gestational age is 1.71％,which is lower than that in the normal children and probably because imperfect central nervous system development in premature infants.

Objective: To investigate the biological behavior and prognosis factors of type B3 thymoma, and evaluate the efficacy of radiotherapy in patients with type B3 thymoma. Methods: A total of 188 consecutive patients with pathologically confirmed type B3 thymoma were treated in Shanghai Chest Hospital from January 2001 to December 2010. According to Masaoka stageing criteria, 35 (20%) were stage, 20 (11%) were stage, 78 (44%) were stage, and 44 (25%) were stage. Myasthenia gravis coexisted in 54 patients. Results: After a median follow-up of 49 months (7-135 months), the 5-year overall survival rate was 84% (100.0%, 89.0%, 86.1% and 59.8% of stages and respectively), and the 5-year progression-free survival rate was 65% (100.0%, 94.0%, 67.3%, and 33.0% of stages, and respectively).The reccurence rate was 22% and the metastatic rate was 7%. In the univariate analysis, tumor size, Masaoka stage, resection margin, radiotherapy and chemotherapy were significantly associated with 5-year overall survival and progression-free survival (P < 0.05). In the multivariate analysis, Masaoka stage, resection margin and radiotherapy were independent prognostic factors of 5-year progression-free survival (P < 0.05). Radiotherapy could improve the regional control rate and the overall survival of Masaoka stages ?-? patients. The intensity-modulated radiation therapy of pleure could probably improve the overall survival of recurrent patients. Conclusion: The major failure mode for type B3 thymoma is the recurrence of pleure. Radiotherapy can improve the regional control rate and the overall survival of the advanced patients. The intensity-modulated radiation therapy may be the best therapy for the postoperative recurrence of pleure. Copyright © 2013 by TUMOR.

The capsule possesses the properties of benefitting Qi, nourishing Yin and activating blood circulation and eliminating stasis. It improves fat metabolism, stimulates function of pancreatic islets, lowers blood glucose as well as alleviates clinical sym(?)oms, with satisfactory results on vascular complications. Improvements also include left myocard(?)l anoxia, left heart function, cerebral blood ci(?)ulation, dilatation of arteries in lower limbs, with anticoagulation and thrombolytic effect.It also reveals certain effect on diabetic nephropathy and early diabetic retinosis.

Objective To investigate the expression changes of stromal interaction molecule 1 (STIM1) in vascular smooth muscle cells in balloon injury of carotid artery in rat in order to explore the proliferation mechanism of vascular smooth muscle cells. Methods A rat model of vascular restenosis induced by injury to the carotid arteries due to balloon dilatation was reproduced in present study. A total of 18 SD rats were randomly divided into 3 groups (6 each): control group, 7-day group and 14-day group after balloon injury to the carotid artery. The expression of STIM1 mRNA in vascular smooth muscle cells was assayed with reverse transcription polymerase chain reaction (RT-PCR), and the STIM1 protein was assessed by immunohistochemistry. The histopathology of the arteries was examined after by HE staining. Results Intima area/media area ratio (IA/MA) was significantly increased in 7-day group after balloon injury than in control group (P

Objective:To study the antibacterial properties of pure titanium treated with benzalkonium chloride solution.Meth-ods:10 mm ×10 mm ×1 mm titanium specimens were processed by the benzalkonium chloride solution at 1%,0.5% and 0.1%respectively followed by treatment in the cultured bacterial suspension,and then the antibacterial properties of the titanium plates were examined.Additionally,the thermal cycling test was carried out for the 1% benzalkonium chloride-treated titanium plates, and subsequently put the plates into cultured bacterial suspension,the duration of antibacterial properties was observed.Results:0.5% and 1% benzalkonium chloride solution-treated titanium plates significantly inhibited the growth of candida albicans(P <0. 05),1% solution was more effective than 0.5% solution.After 1 000 and 2 500 thermal cycling,the pure titanium still retained the antibacterial ability,but the plates treated by 5 000 cycling showed no antibacterial effect.Conclusion:A certain concentration of benzalkonium chloride can make the pure titanium obtain antibacterial properties.The treated plates may maintain the antibacte-rial properties for a minimum of 3 months.

0.05), except PYM-AMS of 1.130 mg/ml and PYM of 300 ?g/ml at 24 h.In the same group and at the same exposure time,higher dose of the corresponding agents showed higher growth inhibition ratio(P0.05).At the same doses 48 h exposure of PYM-AMS or PYM gave higher apoptosis rate than 24 h exposure(P