Objective To explore the relationships between personality traits and internet addiction disorder, as well as which traits would affect internet addiction, and to provide the psychology basis against internet disorder in teenage. Methods The Cattell 16 Personality Factor Questionnaire (16PF) and Chinese Internet Disorder Scale were applied to 409 undergraduates and high school students in Changsha. Use SPSS for windows 11.0 to carry on statistical processing. Results (1) Rule consciousness personality trait certain forecast internet disorder total score ( t = 3.18 ), withdrawal factor ( t = 4.17 ), tolerant factor ( t = 2.64) and time manage factor ( t =3.01 ). (2) Abstractedness personality trait certain forecast internet disorder total score ( t = 2.47 ) and interpersonal and healthy factor ( t = 3.08 ). (3) Warmth personality trait certain forecast forced-internet behavior factor ( t = 2.14 ) and time manage factor ( t = 2.41 ). (4) Social Boldness personality trait certain forecast interpersonal and healthy factor ( t = 2.42). Conclusion Personality traits certain influence internet addiction disorder.

Objective To find the normal range and features for auditory brainstem responses(ABR)of infants within six months,and the basic parameters for identifying the hearing impaired infants.Methods May 2006 to May 2008,60 normal infants and 20 normal adults received the ABR tests.The infants were divided into three groups:group A(6 weeks),group B(3 months)and group C(6 months).Each group consisted of 20 infants(40 ears)and the ABR data were statistically analyzed.Results At 100 dB nHL,the waveⅠ,Ⅲ,Ⅴ latencies of ABR for 6-week infants respectively were 1.49?0.08 ms,4.42?0.16 ms and 6.61?0.25 ms,for 3-month infants,1.47?0.07 ms,4.35?0.20 ms and 6.50?0.25 ms,for 6-month infants,1.45?0.07 ms,4.17?0.15 ms and 6.32?0.22 ms.At 80 dB nHL,the waveⅠ,Ⅲ,Ⅴ latencies of ABR for 6-week infants were 1.63?0.08 ms,4.52?0.17 ms and 6.74?0.26 ms,respectively,for 3-month infants,1.64?0.11 ms,4.44?0.20 ms and 6.67?0.26 ms,for 6-month infants,1.60?0.11 ms,4.27?0.16 ms and 6.43?0.24 ms.As the intensity of stimulating signals decreased,the peak latency of ABR became more delayed.The peak latencies of Ⅲ,Ⅴ waves and interval peak latencies of Ⅰ-Ⅲ and-Ⅴ waves for the infants were more delayed than those for adults,and became shorter with the increase of the infants' ages,but they failed to reach adults' levels even for 6-month infants.However,the length of wave Ⅰ of ABR for infants of different months was close to that for adults.The thresholds of ABR for the infants of different ages were not significantly different,all being close to the values for adults.Conclusion This study has analyzed the ABR wave features for the infants of three ages and this can help to define the reference ranges for the infants and to provide the parameters for early diagnosis of hearing loss among the infants.

Acute mountain sickness ( AMS) is defined as the syndromes which are induced by hypobaric hypoxia within a few hours or days of acute exposure to >2500 m or ascent to a higher altitude than residence .AMS poses a threat to the health and work capability of workers and tourists , as more people enter Tibet and other plateau areas in China .It is known that the incidence of AMS is higher among males and youths than among females , elders and children .The development of AMS is considered to be influenced by the level of sex hormones .Thus, there is the need to find more about the pathogene-sis, pathophysiology of AMS in order to treat and prevention of AMS by studying the relationship between sex hormones and AMS.

The present investigation was undertaken to evaluate the role of the Chinese fruit juice in blocking nitrosamine formation in vitro.A model system simulated the conditions (pH 3.3, 37℃) known, to exist in the stomach, was used in the experiments. Different amount of nitrite and aminopyrine was incubated with Chinese fruit juice or vitamin C solution or buffer solution for 1 hr. 0.1 ml samples were taken and then subjected to the Ames mutagenicity test. The tester strain TA100 of Salmonella typhi-murium was employed for the detection of mutagenicity due to the N-nitro-samine formed from sodium nitrite and aminopyrine. Test samples and bacteria with or without S-9 mixed with molten top agar and poured onto the minimal agar plates. After incubation, the sample, that gave at least a 2-fold increase in induced revertants, compared to spontaneous revertants, was considered to have mutagenic activity.It showed that the concentration of the precursors was at or above 5 mg/ ml with S-9 and without juice. the samples exhibited mutagenic activity. It suggested that the nitrosamine was formed in this system. While the incorporation of Chinese fruit juice was found to inhibit the formation of nitrosamine at 5mg/ml and 8mg/ml. The cultures did not show mutagenic activity. The induced revertant colonies per plate was 252 ? 4.2. The Chin esefruit juice is more effective than that of vitamin C, the induced revertant colonies per plate was 445 ? 81.2, which shows mutagenic. The difference between the cultures of Chinese fruit juice and vitamin C is significant (P

Serum vitamin A, beta-carntene and lgG, IgA, IgM and T-lymphocyte Mastogenesis were measured in lung cancer patients, nonmalignant lung, disease and healthy controls matched lor age, sex and smoking. Mean serum vitamin A of lung cancer (except 5 adenocarcinoma) was significantly lower than that of healthy controls and non-malignant lung disease. Beta-caroiene levels were within the normal range, but mean serum beta-carotene concentration oi lung cancer patients was 24.7% lower, IgA of lung career and non-malignant lung disease patients was significantly higher, IgM and the rate of T-lymphocy te, blastogenesis of patients with lung cancer were lower than that of healthy controls, respectively. Serum IgG was not significant in 3 groups. An inverse relation, between serum vitamin A and IgM of hcalthy controls and arelation between serum vitamin A and IgG of lung cancer patients were observed. These data demonstrate an influence of vitamin A on immune function in lung cancer patients.

Objective To understand the effect of diabetes on the nerve cells injury in hippocampal region by observing the nerve cells changes in this region of ischemia after focal cerebral ischemia reperfusion for rats with diabetes.Methods Sixty healthy male Wister rats were randomly divided into four groups:the control group,sham operation group,normal cerebral ischemia-reperfusion(NIR)group,and diabetic cerebral ischemia-reperfusion(DIR)group.The model of local cerebral ischemia and reperfusion was established by the thread-embolism and cerclage of rats with STZ-induced diabetes.Hematoxylin-eosin(HE)-stained neurons in hippocampal CA1 region were observed by selecting 8 un-overlapped fields under light microscope(?400);the total number of the normal pyramidal neurons with whole cellular organ structure were counted within 100 ?m length in CA1 region.The numbers of positive neurons were presented as mean with standard errors(?s).Significant differences between groups were analyzed by t-test.Results Neuronal loss or apoptotic cells were not found in the control group or sham operation group after observing under light microscope.There were neuronal losses in both NIR and DIR groups,neuronal loss of DIR group being more serious than that of NIR group(P

Objective To investigate the suppression effect of RNA interference(RNAi) on the expression of epidermal growth factor receptor(EGFR) of ovarian cancer SKOV3 cell line. Methods SKOV3 cells were transfected with synthetic EGFR sequence-specific dsRNA by lipofectamin 2000.The expression level of EGFR mRNA and protein were detected by RT-PCR and Western blotting,respectively.The proliferation of transfected SKOV3 cells was detected by WST-1 cell proliferation assay.The abilities of adhesion,migration and invasion were detected by cell adhesion assay,cell migration assay and Matrigel invasion assay,respectively. Results The suppression rates on EGFR mRNA and protein by sequence-specific dsRNA-EGFR were 73.65% and 58.94%,respectively.The cell proliferation was inhibited by 22.54%,35.76% and 31.07%,respectively at 24,48 and 72 h after transfection.The adhesion of sequence-specific dsRNA transfected SKOV3 cells for 30 min and 90 min were reduced by 12.11% and 18.66%,respectively.The migration and invasion of SKOV3 cells were decreased by 25.47% and 22.08%,respectively. Conclusion The down-regulation of EGFR expression of ovarian cancer by sequence-specific dsRNA can lead to the suppression of proliferation,adhesion,migration and invasion of ovarian cancer cells.

The nanobody is a novel antibody fragment,which has beneficial biophysical and pharmacokinetic properties,such as the small molecular weight,high affinity and specificity for antigen.Nanobody is ideally suitable for molecular imaging as a targeting probe that could label antigen at nmol level in vitro.In animal models of xenografted tumor,atherosclerotic plaques and brain disorders,the target tissues were specifically and clearly detected and the high tumor-to-blood (T/B) ratios were obtained.Structural or chemical modified nanobodies will have higher affinity and retention to target tissues,and be convenient for the application of molecular imaging.With the development of the related research,nanobody-based molecular imaging will be gradually transformed into the clinical applications,and play an important role in early diagnosis and therapeutic assessment.