ObjectiveTo observe the effect of the scalp acupunction on children with cerebral palsy.Methods56 cases with cerebral palsy were divided into two groups: the treating group(scalp acupuncture and modern rehabilitation) and the control group(modern rehabilitation only) with 28 cases each. The ADL and the motion function were compared. ResultsThere is a significant defference between the two groups on the ADL, while no significant defference was observed on motor function between the two groups.Conclusions Scalp acupuncture is effective on improvment of ADL of the children with cerebral palsy.

OBJECTIVE To investigate the cognition on hand hygiene and the reasons affecting the hand hygiene performance in hospital.METHODS Medical staff of 358 parsons were randomly selected by questionnaire.RESULTS It was higher cognition among medical staff on hand washing indication learned from Standards of Hand Hygiene among Medical Personnel in Medical Institutions(draft for soliciting opinions) issued by the Ministry of Health.The cognition coincidence of hand sterilization indication was 68.04%,but existed some less cognition or miscognition in this field among them.The main factors affecting the hand hygiene performance were busy at work,to which was the primary cause to affect use of stream water to wash hands.The concept that using stream water wash hand is reliable is the primary cause influence medical staffs to use alcohol scrape hand.CONCLUSIONS The nosocomial infection monitoring,guidance and control should be emphasized in the field of more use of the fast hand antiseptics instead washing hands and supervising the relevance of the amount of hand hygiene product consumpsion to nosocomial infections.

Alzheimers disease (AD) is a progressively and retrogressively neurodegenerative disorder of central nervous system severely threatening the aged. The studies of animal models, which share the very similar neuropathological characteristics with human, provide tools for the understanding of the etiology, mechanisms and medicine screening of AD. The distribution and chemical composition of amyloid-beta (A- beta) peptide -positive deposits were investigated in various non-human primate models. Various proportions of A- beta deposits contained cholinesterase activities, apolipoprotein E(ApoE) and alpha1-antichymotrypsin immunoreactivity. Alterations in the basal forebrain cholinergic system have been widely studied, but the magnitude of decline and relationship to cognitive impairment are still a matter of debate. This article also includes the latest research of the changing on tau, subtle structural alterations in axons, and neurofibrillary tangles (NFT) in the brain tissues of the non-human primate models.

Aged ; Aged, 80 and over ; Anthracosis ; complications ; therapy ; Humans ; Male ; Middle Aged ; Pulmonary Heart Disease ; complications ; therapy

Animals ; Breast Neoplasms ; diagnosis ; genetics ; DNA, Mitochondrial ; genetics ; Electron Transport Complex IV ; genetics ; Humans ; Loss of Heterozygosity ; Microsatellite Instability ; Mitochondrial Proteins ; genetics ; Mutation

BACKGROUND: The reaction of osteoblasts is important role in the reconstruction of osteocytes, and the mechanism of bone remodeling can be known from the cytological level through analyzing the mechanical reaction of osteoblasts under different loading.OBJECTIVE: To analyze the effects of low frequency vibration on the proliferation and differentiation as well as the matrix excretion of cultured human osteoblasts.DESIGN: A comparative observation.SETTING: Department of Orthopaedics, Nanfang Hospital of Southern Medical University.MATERIALS: The experiment was carried out in the laboratory of Nanfang Hospital from January to December in 2002. Human osteoblasts were isolated from the iliac cancellous bone of adults.METHODS: The osteoblasts were cultured for 48 hours, and then treated with low frequency vibration of 0.1, 0.2, 0.5, 2 and 5 Hz. The proliferation of the osteoblasts was detected with flow cytometry, and the activity of alkaline phosphatase (ALP) and content of osteocalcin were assayed by spectrophotometric methods and radioimmunoassay respectively.MAIN OUTCOME MEASURES: ① Effects of low frequency vibration on the ALP activity and osteocalcin excretion of osteoblasts; ② Effects of low frequency vibration on the proliferation of osteoblasts detected with flow cytometry.RESULTS: ① 0.2 and 0.5 Hz vibrations could markedly increase the ALP activity of osteoblast (P ＜ 0.01), but that of 5 Hz obviously decreased the ALP activity (P ＜ 0.01). 0.1 and 2 Hz vibrations had no obvious effects on the ALP activity of osteoblasts. ② 0.2 and 0.5 Hz vibration could significantly increase number of osteoblasts at S phase from 10.4% to 12.45% and 16.12%, and the proliferative index increased from 20.14% to 26.21% and 28.75%; 0.1 and 2 Hz vibration has not obvious effects in osteoblast proliferation index; 5 Hz vibration obviously decreased the proliferative index value to 13.22% (P ＜ 0.05). 0.1 and 2 Hz vibrations had no obvious effects on the proliferative index of osteoblasts (P ＞ 0.05). ③ 0.2 and 0.5 Hz vibrations could obviously increase the amount of osteocalcin excretion to 1.87 μg/L and 2.47 μg/L(P ＜ 0.05), but 2 and 5 Hz vibrations decrease the amount of osteocalcin excretion (P ＜ 0.05), and that of 0.1 Hz had no obvious effect on amount of osteocalcin excretion (P ＞ 0.05).CONCLUSION: Low frequency vibrations of 0.2-0.5 Hz could accelerate the proliferation and differentiation as well as the excretions of active materials of osteoblasts, and it plays an instructive role for low frequency vibration to treat fracture.

Objective: To explore the relationship between strain rate parameter and left ventricular diastolic function (LVDF) in patients with cardiac resynchronization therapy (CRT) by two-dimensional speckle tracking echocardiography (2D-STE).
Methods: A total of 70 heart failure (HF) patients with CRT were studied. The early left ventricular diastolic strain rate was obtained by 2D-STE technology, the differences of left ventricular diastolic functional parameters to various CRT echocardiographic responses were observed. The area of relevant parameters under ROC curve was calculated by early left ventricular diastolic strain rate for predicting CRT echocardiographic response.
Results: There were 41 patients presented as CRT echocardiographic responders at 12 months of follow-up period, 29 patients were non-responders. The most parameters of LVDF were signiifcantly improved than the base line in 41 responders. The maximum area of 2D-STE derived parameters under ROC curve was obtained by LSRE predicted CRT echocardiographic response
Conclusion: LSRE had greater value for predicting CRT echocardiographic response, it might be used as a sensitive index for evaluating LVDF in HF patients.

Objective To study the expression of proliferating cell nuclear antigen (PCNA), CDK4 and P16 on rat hepatocellular carcinoma by millimeter wave radiation. Methods Fourty male Wistar rats were randomly divided into four groups. Group one to three were feeded by diethylnitrosamine (DEN). Group one was a tumor control group. In group two and three the liver was directly radiated by 35.8 GHz, 100 mW/cm 2 millimeter wave for 20 min, twice a week for 10 or 5 weeks. Group four was a normal control radiated group. Fourteen weeks later all rats were sacrificed to undergo serological test and immunohistochemical stain of liver. Results The serum levels of ? glutamyltransferase in group two and three were lower than that in group one. Adenocarcinoma was only existed in group one by histological examination of liver tissue. Other groups of DEN exposure only had basophilic and eosinophilic nodules. Liver tissue expression of PCNA and CDK4 in group two and three were significantly lower than in group one, but the expression of P16 in group two and three was higher than that in group one. Conclusions Radiation with millimeter wave can partially inhibit cell proliferation and suppress the DEN induced hepatocellular carcinoma in rats.