OBJECTIVE:To investigate effect of valsanan on heart function in patients with chronic heart failure(CHF).METHODS:A total of 113 CHF patients were assigned into 2 groups.Both group received routine therapy and observation group were additionally treated with valsanan.Clinical efficacies of 2 groups before and after treatment were observed in terms of cardiac function classification,LVIDD,SV,CO,LVEF and 6 min walking test.RESULTS:In observation group,the incidence of improvement of the cardiac function was higher than in control group(P

Autophagy can be activated after cerebral ischemia and involved in the occurrence and development of ischemic brain injury. Therefore, the study of autophagy related signal transduction pathways may provide a new therapeutic target for ischemic brain injury. This article reviews the signal transduction pathways of autophagy after cerebral ischemia.

Due to the complicated and changeable clinical symptoms,and inexact pathogenesis,leukemia was facing huge challenges in diagnosis and therapy.Aptamer could be selected through CELL-SELEX.The aptamer not only display high affinity and specificity to target cell,but also it could discriminate the specific stage of cell,such as different classification,normal and tumor cells.So CELL-SELEX has great potention in the researches of leukemia.This paper systematically summarized the advantages of CELL-SELEX,the application in specific leukemia diagnosis,targeted therapy and the discovery of cancer biomarkers.(Chin J Lab Med,2013,36:377-379)

OBJECTIVE:To probe into the status quo and requirements of drug utilization in Haiqin Sanatorium Area of Nanjing Military Region Hangzhou Sanatorium.METHODS:The drug utilization in 710 convalescents in Sanatorium Area from May 2007 to April 2008 was analyzed statistically.RESULTS:A total of 10 categories of drugs were used in the 710 convalescents,chiefly including anti-cold drugs,antihypertensive drugs,drugs for external use,antimicrobial agents,ophthalmic drugs,otorhinolaryngological drugs,vitamins and gastrointestinal drugs.The rate of drug use conforming diagnosis was 87.32%.Oral administration occupied 71.13%.CONCLUSION:The drug utilization in convalescents in Haiqin Sanatorium Area is basically rational.However,there are also some unreasonable points in the drug utilization which remain to be tackled further.

OBJECTIVE To investigate the drug resistance status of Klebsiella pneumoniae,Escherichia coli and(Enterobacter cloacae) for reasonable use of antibiotics. METHODS After genus identification,drug resistance profile was analyzed by K-B method. RESULTS The predominant isolates from Enterobacteriaceae were(K.penumoniae) and E.coli.The resistant rate of the three commonly encountered bacteria in Enterobacteriaceae to antibiotics ascended yearly,with increasing isolates harboring ESBLs from 16.5%(2000) to 32.4%(2004). CONCLUSIONS Rational use of antibiotics should be obey the results of laboratory antibiotics susceptibility test to control the increasing trend and forbid outbreak of resistance.

OBJECTIVE To investigate drug resistance status of common bacteria to ciprofloxacin in hospital for reasonable use of antibiotics.METHODS After genus identification,drug resistance profile was analyzed by K-B method.RESULTS The resistant rate of common bacteria to ciprofloxacin ascended yearly from 40.8%(2000) to(51.7%)(2004),which predominated with Escherichia coli,coagulase negative Staphylococcus and S.aureus,respectively.CONCLUSIONS Rational use of fluoroquinolones should be obey the results of laboratory antibiotics suceptibility test to control the increasing trend of resistance.

Psychological disorder has a high morbidity rate among patients with cardiovascular disease. Patients with cardiovascular disease associated with depression, especially post myocardial infarction and acute coronary syndromes associated with psychological disorder, have not only a poor prognosis, but also a high mortality . Therefore, comprehensive and perfect intervention is extremely important for patients with cardiovascular disease associated with psychological disorder. The intervention for such kind patients needs an integrated disease management. It needs to consolidate the treatment of body's disease to strengthen second-level prevention of heart disease, and pay attention to the intervention for psychological disorder as well.This paper aims to provide reference for reducing the negative effects of psychological illness in patients with cardiovascular disease during clinical treatment process, and make prospects about the significance of double heart medical model, through summarizing the interaction effects of psychological factors on patients with coronary heart disease, the significance ofdouble heartnursing mode and the specific nursing measures.

In recent years, there is still an unbridgeable area in the early diagnosis of thyroid carcinoma.Many scholars are committed to the research of molecular markers, and find that RNA markers associated with thyroid carcinoma are microRNAs, human telomerase reverse transcripase, trefail factor 3 and HMGA2 genes.Different genes are closely related to the occurrence, development of different types of thyroid cancers, and some genes may even affect the transfer of thyroid carcinoma.

Establishing harmonious physician-patient relationship is importamt to set up the harmonious society.Some hospitals try in recent years a line the single disease grows the price control charges,which suggest its importamt role in seting up harmonious physician-patient relationship,and greatly attract public's concern as the new measure in this aspect.

OBJECTIVE: To establish the HPLC method for the determination of chloramphenicol and dapsone in compound chloramphenicol cream. METHODS: The analytical column was Hypersil ODS and the mobile phase consisted of 0.1%sodium heptanesulfonate solution (a mixture of 0.1% sodium heptanesulfonate 500 mL and dimethylformamide 5 mL plus acetic acid glacial 0.5 mL)-acetonitrile (75∶25) at a flow rate of 1.0 mL?min-1. The detection wavelength was 272 nm and the sample size was 20 ?L. RESULTS: The linear ranges of chloramphenicol and dapsone were 25.59～153.54 ?g?mL-1 (r=0.999 9) and 12.57～75.42 ?g?mL-1 (r=0.999 9), respectively. The average recovery rates were 100.62% (RSD=0.92%) and 100.93% (RSD=1.22%), respectively. CONCLUSION: The method is simple and accurate and can be used for the quality control of compound chloramphenicol cream.