Objective: This research mainly concerns the relationship between the behavior preference in network game and internet addiction for players. Methods: This study surveyed 324 players with Behavior Preference in Network Game Questionnaire(BPNGQ) and Internet Addiction Questionnaire(IAQ). Results: There was significant difference in playtime and years of internet for achievement and killer. All scores in factors of BPNGQ were significantly correlated with IAQ. All scores in these factors excluding attitude and emotion of aggression were significantly higher for addictive players. All scores in internet addiction of four typing players excluding achievement and killer were significantly different. Conclusion: Scores of BPNGQ for players could forecast internet addiction. Players of different types display various addiction in network game. Too many behaviors of aggression in games lead to internet addiction for players.

Polyporus Umbellatus polysaccharide(PUP) is a new medicine made by our institute. A model of aged mice was prepared by placing 2-month mice in an ozone circumstance (O_3 conoentration 1. 298 ppm) for 10 days. After the aged mice was fed with PUP for 10 days, the average body weight, body temperature and thymus coefficient can be increased to normal level, superoxide dismutase (SOD) activity of red cells and catalase activity of liver were increased, and liver lipoperoxide (LOP) reduced. It is suggested that PUP may be a good anti-aging agent.

Objective To investigate the restricting factors of long-term care of stroke survivors in community.Methods The phenomenological methodology was adopted in the study.Sixteen stroke survivors were interviewed.Data were analyzed by Colaizzi's method.Results Three themes were found,including physical barriers,incorrect attitude towards disease,and defects of community health service.Conclusions On the basis of improving technology and ability,medical workers in community should consider of supporting the patients with information of disease and useful resources in a variety of ways,and improving the patients health literacy.

Objective To analyze the clinical diagnosis and treatment of recurrent cancer in gastric remnant.Methods Clinical data of 48 patients who underwent surgical re-operation because of recurrent carcinoma within gastric remnant after radical resection of gastric cancer were analyzed retrospectively.Results All 48 cases were recurrent cancer in gastric stump.The time of recurrence was 6-36 months after first radical resection.After reoperation,the mean survival time of 28 patients(58.3%)who underwent radical resection was 40 months;the mean survival time of 20 patients(41.7%)who received palliative treatment was 14.8 months(P

Objective To study the causes of local recurrence,and diagnosis and treatment outcome of recurrent colorectal cancer.Methods The clinical data of 60 patients with recurrent colorectal cancer treated during 10 years in our hospital were analyzed retrospectively.Results Among the 60 cases,40 cases(66.7 %) had recurrence within 2 years after operation.Recurrence in anastomotic stoma,perineum,abdominal incision occurred and in the abdominal cavity and pelvic cavity in 15,10,7 and 20 cases respectively,and liver metastases were found in 8 cases.All patients underwent reoperation including curative surgery for 38 patients and palliative operation for 22 patients.After radical reoperation the 1-,3-,and 5-year survival rate was 93.6 %,48.8 %,and 36.3 %,respectively,and after palliative reoperation was 54.5 %,0 %,and 0 %,respectively.Conclusions Emphasizing the application of no-tumor touch technique,resection of adequate amount of bowel,performing complete lymphadenectomy and removal of micrometastatic lesions are the major measures to prevent recurrence of rectal cancer after operation.Integrative therapy regimens,of which surgical treatment is the major component,should be considered according to the location of recurrence and the clinical staging of the recurrent cases.

According to the study experience when the author was invited as a visiting scholar in Edinburgh Napier University,also based on the further discussion with local research team members of stroke,the author would like to introduce the multidisciplinary research team with same professional field being established in Scotland,finally in order to give us inspiration how to develop an efficient,long-term and stable research team in China,and which model whether local universities innovative research team or innovative regional research team is much more suitable for our country.The author had participated in stroke team research activities during the Edinburgh Napier University visit;and have some basic understanding to their research ideas and patterns of work after communication and exchanging ideas with team members.Particularly,multidisciplinary research collaboration model based on voluntary organization across entire Scotland is worth to learn for us.It also would inspire the university research terms in their construction in China.

Objective To investigate the relationship between positive experience and social support of spouses' of community stroke patients.Methods 100 stroke patients and their spouses in two communities were conveniently selected and investigated about their positive experience and social support with General Information Questionnaire (GIQ),Modified Barthel Index(MBI),Positive Aspects of Caregiver (PAC),Perceived Social Support Scale (PSSS).Results The mean score of the spouses' positive experience was (22.74±5.85); the dimension of serf-affirmation was (15.82±3.84),higher than the dimension of life expectation(6.92±2.95).The scores of the spouses' positive experience were significantly different among age,gender,education of spouses and age and ability of daily life of stroke patients; the score of the spouses' positive experience was positively correlated with social support,subjective support,utility of support;multiple linear regression showed that age of spouses,ability of daily life of stroke patients and utility of support were influential important factors of spouses' positive experience.Conclusions The spouses' positive experience was below average level,we must pay more attention to improve it,utility of support can improve spouses' positive experience.

Objective To investigate the incidence of cholecystolithiasis in 1iver cirrhosis and analyze its influencing factors and clinical significance. Methods We selected 128 patients with 1iver cirrhosis who were di-agnosed and treated in the First Affiliated Hospital of Henan University of Science and Technology from Oct. 2014 to Aug. 2015 as the observation group. Meanwhile, 140 cases received medical examination served as the control group. The liver cirrhosis group were divided into class A (group A), class B (group B), class C (group C) ac-cording to the Child-Pugh grades. We measured the levels of fasting serum albumin (ALB) and cholecystokinin (CCK) of all subjects. The relationship of cholecystolithiasis in 1iver cirrhosis to gender, liver function Child-Pugh classification, ascites, levels of ALB and CCK was analyzed. Results (1) Univariate analysis revealed: compared with the control group, the incidence of cholecystolithiasis in 1iver cirrhosis group was higher (35.2%vs 8.6%, P<0.05). With increase of the degree of liver damage, the incidence of cholecystolithiasis increased. And the incidence of cholecystolithiasis in group B and group C were both higher than that of group A (group A 22.9% vs group B 35.0%vs group C 50.0%, P<0.05). The incidence of cholecystolithiasis in the 1iver cirrhosis group with ascites was significantly higher than that of the control group (38.6% vs 22.2% vs 8.6%, P < 0.05). With increase of the degree of liver damage, the level of ALB in liver cirrhosis group decreased compared with the control group (P<0.05), while the level of CCK was obviously higher than that of the control group (P < 0.05). The morbidity rate did not differ between the males and females with cirrhosis (P>0.05). (2) Multiple Logistic regression analysis re-vealed that the level of ALB is the main influencing factor (P<0.05). Other factors were not statistically significant. Conclusion Cholecystolithiasis frequently occurs in patients with liver cirrhosis, which has no correlation with the gender of patient, but it correlates with liver function, ascites, the levels of ALB and CCK. Among of them, the level of ALB is the main influencing factor.

Aim To investigate the effects of isoliquiri-tigenin ( ISL) on anti-angiogenesis both in vitro and in vivo and its mechanisms. Methods We assessed the antiangiogenic activities of ISL on proliferation viabili-ty, migration and tube formation of human microvascu-lar endothelial cell line-1 (HMEC-1) in vitro. The cell proliferation viability was assessed using the Sulforho-damine B ( SRB ) assay. Modified Boyden Transwell chamber assay was done to study the effect of ISL on HMEC-1 cells migration. 2′, 7′-dichlorofluorescein di-acetate ( DCFH-DA) was used to measure the levels of intracellular reactive oxygen species ( ROS ) , which was induced by VEGF. Metalloproteinase-2 ( MMP-2 ) and metalloproteinase-9 ( MMP-9 ) expressions by HMEC-1 cells were assessed through gelatin zymogra-phy assay. HMEC-1 cells cycle was detected by flow cytometry. Moreover, we investigated the in vivo anti-angiogenic activity of ISL on chicken embryos nap al-lantoic membrane model ( CAM ) . Results ISL con-centration-dependently inhibited the growth of HMEC-1 cells as well as SW620 and A549 cells. ISL signifi-cantly and concentration-dependently suppressed the migration activity of HMEC-1 cells. Tube sample struc-ture formation further confirmed the effect of ISL on an-ti-angiogenesis. Moreover, ISL also inhibited intracel-lular ROS level, MMP-2 and MMP-9 expression by HMEC-1 cells. ISL induced endothelial cell apoptosis at a low concentration ( ISL 12 . 5 μmol · L-1 ) and blocked the cells in S phase of mitosis at higher con-centrations ( ISL 25~100 μmol·L-1 ) . Furthermore, ISL distinctly inhibited the angiogenesis of chick em-bryos in vivo. Conclusions ISL has anti-tumor and angiogenesis effects on HMEC-1 cells. The mechanism may be related to intracellular ROS scavenging and ap-optosis induction of HMEC-1 cells.

OBJECTIVE To invesigate the prevalence of SHV-producing Enterobacteriaceae isolates in Hunan Province and identify the subtype of SHV encoding gene and the epidemiological aspect of SHV-producing isolates. METHODS Isolates were collected and identified as well as subjected to ESBLs detection.PCR and DNA sequencing were used to determine the genotype of SHV enzymes.The homology of SHV-producing strains were detected by RAPD. RESULTS Twenty-six of 171 Enterobacteriaceae isolates were confirmed to produce blaSHV genes,and 5 subtypes of SHV-type ?-lactamases were determined,including 9 strains of SHV-28,7 strains of SHV-12,7 strains of SHV-1,2 strains of SHV-11 and 1 strain of SHV-5.There were 6 RAPD types in 19 isolates of SHV-producing Klebsiella pneumoniae,5 RAPD types in 5 isolates of SHV-producing Enterobacter cloacae. CONCLUSIONS SHV-12 is the predominant genotype of SHV ESBLs producing Enterobacteriaceae in Hunan Province,and clone spread has played a certain role in SHV-type ?-lactamase producing K.pneumoniae.SHV ESBLs are not found in Escherichia coli.