Objective: To study NOS activities in serum of rats under different stresses. Methods: Two models of stress were made in rats, one is the conventional forced swimming model (including 12 rats), and the other is a social defeated paradigm (including 12 rats). The activities of NOS of rats under each model and of one control group (10 rats) were measured. Results: Compared with control, the NOS activity under social defeated paradigm was lower (15.36?1.74/17.11?1.67 U/ml, t=2.32, P

Objective: To study the change of NO (nitric oxide) and NOS (nitric oxide synthase) in brain of rats induced by acute stress Method: The concentration of NO and NOS in frontal lob, hippcampus, midbrain and hypothalamus were measured in 1 and 3 hours after stress respectively Results: The concentration of NO and NOS in hippcampus and hypothalamus were significantly higher than that of control group at 1 hour after stress (P

Objective:To examine the effects of psychological and physiological stress on hippocampal extracellular monoamine content under simulated high-altitude hypobaric hypoxia.Methods:The male SD rats were randomly divided into ten groups:control(Con group), hypoxia(Hy group), psychological stress(twice/day)(Ps1group), psychological stress(twice/day?2day)(Ps2 group), psychological stress(twice/day) + hypoxia(Ps1+Hy group), psychological stress(twice/day? 2day) +hypoxia(Ps2+Hy group), physiological stress(twice/day)(Ph1group), physiological stress(twice/day?2day)(Ph2 group), physiological stress(twice/day) + hypoxia(Ph1+Hy group), physiological stress(twice/day?2day) + hypoxia(Ph2+Hy group).The contents of norepinephrine(NE), dopamine(DA) and 5-hydroxytryptamine(5-HT) in extracellular fluid of hippocampus collected by push-pull perfusion were determined by high performance liquid chromatography with electrichemical detection(HPLC-ECD).Results:①Compared with the Con group, the content of NE was significantly lower in Hy, Ps1+Hy and Ph2+Hy groups(P

Objective: To investigate the influence of live ammunition maneuver on mental health of soldiers in mobile troop. Method: 428 soldiers in mobile troop participating live ammunition maneuver on plateau were included. 198 soldiers in another troop garrisoned on plateau were collected as control. SCL-90, EPQ and SSRS (social support rating scale) were applied to soldiers in mobile troop before and after ammunition, and also to controls. Results: After ammunition, numbers of positive items and all 9 subscores of SCL-90 increased than before (P

Objective: To study the rate of PTSD after traffic accident in orthopedics and do retrospective analysis. Method: Eighty one patients wounded by traffic accident in orthopedic ward were surveyed. Acute stress reaction (ASR) and posttraumatic stress disorder (PTSD) were diagnosed according to CCMD-2-R criteria. SCL-90 and EPQ were also applied. Results: 33 cases (40.7%) were diagnosed as ASR and 31 (38.3%) as PTSD among all subjects. SCL-90 score and nearly all subscores were higher in patients with PTSD than patients without (P

Objective: To study the changes of serum interleukin levels and immune function of rats under stress. Method: 18 male rats were randomly divided into two groups, stress group (10) received "psychosocial"stress and their serum were taken after 1 hour; control group (8). Serum interleukin 2, 6, 8 and cortisol levels of all subjects were determined. Results: Compared with control group, the level of serum cortisol increased after stress (6.51?2.5/3.76?0.4 (ug/dl, t=3.45, P

Objective: To investigate the effects of forced swimming on NO and NOS in brain of mice. Method: Sixty mice were assigned into three groups randomly: two stress groups, the content of NO and NOS were determined at 1hour and 2 hours after forced swimming respectively, and one control group. NO was tested by chromatic comparison. NOS was tested by enzyme assay. Results: The level of NO in stress group tested at 1 hour was significantly lower than that in control group (17.38 ?5.63/27.47?15.16 (mol/gprot, t=2.67, p

Objective: to explore the influence of traffic accident psychologically and physiologically Method:120 inpatients fractured in traffic accident (study group) and 67 fractured inpatients (control group) from other reasons were collected The study group was divided into those with stable character (with N score of EPQ56) All subjects completed SCL-90 Their IL-1?, IL-2, IL-8 and cortisol level were measured Result: The total score and subscores of SCL-90 of study group were significantly higher than that of control The serum levels of IL-1?, IL-2, IL-8 were lower than that of control, while cortisol level was higher than control The interleukin levels of stable group were higher than that of unstable group Conclusion:Patients injured by traffic accident have poor health both mentally and physically, especially for those with unstable character

Objective: To investigate the effects of paradoxical sleep deprivation (SD) on serum cytokine levels of Sprague-Dawley rats Method:Paradoxical sleep deprivation was induced in male rats by housing them on small platforms over water Controls were housed either in normal cages (CC) or in tanks with large platforms (TC) After SD for different lengths of time, serum levels of IL-2, 6, 8, TNF-? and cortisol of all groups were measured by double antibodies radio-immune assay Results: Serum level of TNF-? and cortisol of SD24h group were higher than that of CC (P

Objective: To study the change of cyclic nucleotide in brain of rats induced by acute stress Method: The concentration of cAMP and cGMP in frontal lob, hippcampus, midbrain and hypothalamus were measured in 1 and 3 hours after stress respectively Results:The concentration of cGMP in hippcampus and hypothalamus were significantly higher than that of control group at 1 hour after stress (P