82 serum samples from the blood donors were comparatively tested by using different reagent kits and two methods (enzyme-linked immunosorbent assay, ELISA anl passive hemagglutination inhibition assay, PHA). The results are as follows: the positive rates of A1, A2 , A3 , A4-1 and B (containing mixed antigens) are 56.09%, 71.95%, 47.56%, 63.41% and 59.75% respectively; and the positive rates of A4-2 and A4-3 (containing single antigens) are 41.46% and 62.19% respectively. The results of testing 8 of all the serum samples by four-gene-recombinent immunoblot assay (RIBA) confirmed that the results of A1, A2, A3, A4-1 and A4-3 were consistent with them. The 76th sample with positive result when tested by RIBA had a negative result when tested by RIBA, while the 82th sample with negative result when tested by RIBA had posi tive one when tested by B. It is suggested that reagent kits made in China left much to be desired in antigen-matching, compactness and cut-off value determining, though they could screen out most of the donors with positive anti-HCV results.

Objective To investigate the image manipulation of the lower limb phlebography in Innova4100. Materials and Methods All the patients were injected contrast agent by high pressure syringe through the vein of the dorsum of foot. The phlebography images were obtained by controlling the movement speed of the bed and blood flow. Results 95 percent of the images were excellent with good contrast and clear background. The blood vessel and the pathological changes were clear. Conclusion Adv Paste is a good software which can connected images to form complete phlebograms for lower limbs.

OBJECTIVE: At present the function of Fas and Bcl-2 gene in the pathogenesis of myasthenia gravis has gained more attention and more studies have been extensively made inside and outside, to investigate the modulation of Fas and Bcl-2 gene expression is of extremely significance for exploring the pathogenesis and treatment of myasthenia gravis.DATA SOURCE: The related articles published in English from January 1990 to December 2004 were computer searched in Medline database using the terms of "Fas", "Bcl-2", "Myasthenia Gravis" and "gene".STUDY SELECTION: The data were identified preliminarily and literatures on the relationship between Fas and Bcl-2 gene and myasthenia gravis, as well as on Fas and Bcl-2 gene and genotherapy were remained for searching the full content.DATA EXTRACTION: A great deal of literatures about Fas and Bcl-2 gene were searched, 21 of which were studies on Fas and Bcl-2 gene and myasthenia gravis, and 32 on myasthenia gravis related genotherapy. A total of 20 representative articles were selected for analysis in this study.DATA SYNTHESIS: Eight out of 20 literatures were about the relationship between Fas gene and myasthenia gravis, 5 about the Bcl-2 gene and myasthenia gravis, 7 about the genotherapy in the treatment of myasthenia gravis and immune diseases.CONCLUSION: Abnormal Fas and Bcl-2 gene modulation and uncontrollable expression were closely associated with apoptosis defect of T lymphocyte and thymocytes, and even closely related to the pathogenesis of myasthenia gravis. To explore pathogenesis of myasthenia gravis at gene level by inducing cell apoptosis and depressing self-antibody production, would provide new means for the treatment of myasthenia gravis.

Objective To evaluate the difference of diagnostic efficacy and complications between 18 and 20 gauge(G)core needle in transcutaneous lung biopsies under CT-guided.Methods Transcutaneous lung biopsies were performed using 18G (group A) and 20G(group B) core needles in 60 cases respectively.Their diagnostic efficacy and complications were compared.Results In group A, 45 of48 patients with lung cancer were accurately diagnosed by biopsies( 93.8% ) and another 3 cases were false-negative( 6.2%). 10 of 12 patients with benign lesions were diagnosed by biopsy as well . In group B , 42 of 46 patients with lung cancer were diagnosed by biopsy ( 91.3%) and another 4cases were false-negative (8.7%) .10 of 14 patients with benign lesions were accurately diagnosed by biopsies . Pneumothorax was found in 20 cases in group A , only 2 cases of which were handled with drainage,whereas a little pneumothorax was found in 8 cases in group B without special handling.No differences between two groups in diagnostic accuracy,false-negative rate and pneumothorax rate found . Conclusion There are more complicated with pneumothorax and much severe degree in group A than that in group B,but the diagnostic accuracy,false-negative rate and pneumothorax rate are not significantly different between two groups.

Objective To explore the diagnosis and treatment of Mycobacterium tuberculosis infection in a child with severe β Mediterranean anemia after allogeneic hematopoietic stem cell transplantation (allo-HSCT).Methods The clinical data of a child with severe β mediterranean anemia who had Mycobacterium tuberculosis after allo-HSCT were retrospectively analyzed.The pertinent literatures were reviewed.Results Six-year-old girl with Mediterranean anemia was infected by Mycobacterium tuberculosis after allo-HSCT.After anti tuberculosis treatment by HRZE (isoniazid,rifampicin,pyrazinamide and ethambutol),the condition was improved.Conclusion It is rare of Mycobacterium tuberculosis infection after allo-HSCT,which needs timely diagnose and treatment.

Mitochondria is an important part of cell oxidation, but oxidative stress in sepsis as end product of inflammation will cause changes of mitochondria, leading to organ damage. In general, antioxidant defense system fights oxidative stress through the interaction and prevents mitochondrial injury. Oxidative stress plays a important role in organ dysfunction. Studies have found that the targeted therapies for mitochondrial antioxidants may be a new strategy of multiple organ failure caused by sepsis. This review aims to summarize researches on oxidative stress of mitochondrial injury mechanism and targeted antioxidants in sepsis .

OBJECTIVE: To compare the therapeutic effect of postoperative radiotherapy with surgery alone in advanced supraglottic laryngeal squamous cell carcinoma (LSCC). METHOD: According to the treatment methods, 59 patients with advanced supraglottic LSCC were divided into surgery plus radiotherapy group (S+R group) (33 cases) and surgical group (S group) (26 cases). In S+R group, total laryngectomy was performed on 27 patients and partial laryngectomy on 6 patients, 27 of whom underwent lateral neck dissections; while in S group, total laryngectomy was performed on 23 patients and partial laryngectomy on 3 patients, 24 of whom underwent lateral neck dissections. RESULT: The 3-year overall survival rate of S+R and S group were 62.6% and 62.6% respectively, and the 5-year overall survival rate of S+R and S group were 43.8% and 40.5% respectively. But there was no statistically significant difference of survival rate between two groups by Log-rank test (P > 0.05). In S+R group, recurrence happened in 5 cases and the recurrent rate was 15.1%; in S group, recurrence happened in 10 patients and the recurrent rate was 38.5%, statistically significant difference was found between the two recurrent rates (P < 0.05). CONCLUSION Surgery combined with postoperative radiotherapy could not improve the 3-year-or 5-year overall survival rate, but could significantly reduced tumor recurrence rate.
Adult ; Aged ; Carcinoma, Squamous Cell ; pathology ; radiotherapy ; surgery ; Combined Modality Therapy ; Female ; Glottis ; pathology ; Head and Neck Neoplasms ; pathology ; radiotherapy ; surgery ; Humans ; Laryngeal Neoplasms ; pathology ; radiotherapy ; surgery ; Laryngectomy ; Male ; Middle Aged ; Neck Dissection ; Squamous Cell Carcinoma of Head and Neck ; Survival Rate ; Treatment Outcome

OBJECTIVE: This study was to evaluate the patterns of central lymph nodes metastasis, by analyzing the results of surgery in clinical NO (cNO) papillary thyroid carcinoma (PTC). METHOD: We retrospectively studied the effect of surgical treatment among 123 cN0 PTC patients. All the patients underwent central lymph node dissection; 47 patients underwent ipsilateral neck dissection and 9 patients underwent bilateral neck dissection. RE- SULT: Seventy-eight cases (63.4%)were found positive central lymph node, which included 34 cases (27.6%) bilateral positive central lymph node. Central lymph node metastases correlated with age < 45 years, extrathyroidal extension, surrounding tissue invasion and tumor size grade (P < 0.05), were the independent risk factors of central lymph node metastasis. CONCLUSION Age < 45 years, extrathyroidal extension and surrounding tissue invasion were the independent risk factors of central lymph node metastasis. For cNo patients with PTC, primary excision as well as central neck dissection was recommended; according to the results of intraoperative frozen and situation, further process were made.
Adult ; Carcinoma ; pathology ; Carcinoma, Papillary ; Humans ; Lymphatic Metastasis ; Neck Dissection ; Retrospective Studies ; Risk Factors ; Thyroid Cancer, Papillary ; Thyroid Neoplasms ; pathology

Specialists in the south of the five ridges think that bubonic plague is caused by blood stasis due to noxious heat;they exactly use Wang Qingren' Decoction of Detoxification and Promoting Blood Flow modified for detoxification,clearing heat and removing blood stasis.Peach Seed and Safflower are principal drugs of this decoction;using Rhinoceros Horn,Antelope Horn and Tibet Safflower to clear noxious heat for protecting the heart.The key to make curative effect is that this decoction needs to be obeyed urgently frequently and modified according sighs.They have found the substitutes of precious Rhinoceros Horn,Antelope Horn and Tibet Safflower.

Objective　To study on dynamic laws of the human plague in China and America.Methods　The autoregressive models of human plague dynamics were set up.Results　Human plague were 7 923 and 398 cases in China and America between 1950 and 1998,respectively.The dynamic models ARIMA(1,2,0) of human plague in China and AR(1) in America between 1950 and 1998 were obtained,resectively.Conclusions　These general trends on the cases of human plague occurred have increased in China and relative stabilized in America between 1999 and 2001.