Certification ; Laboratories

Experiments were caried out on An.minimus F98 in laboratory conditions of 22+/- 1°C, 25+/- 1°C and 28 +/- 1°C, 70-80% RH. The time required for egg stage and egg hatching rate were negatively correlated with temperature. At 22 +/- 1°C, egg hatching rate was 84.1 %, the duration of the egg stage was 3-4 days. At 25+/-1°C and 28 +/-1oC, egg hatching rate was 77.3% and 65.5%, respectively, the time of the egg stage were 2-3 days. At higher temperature, the duration of larvae stage was shorter than at the lower temperature. 25°C was optimum temperature for larvae with 38% of larvae developed to adults. 1 day after molting, mosquitoes started feeding at all tested temperatures. At 22°C, blood feeding rate higher than at the higher temperatures. Number of eggs and egg hatching rate of mosquitoes that fed human blood were higher than mosquitoes fed on mice
Anopheles ; Temperature ; Blood ; Laboratories

The evaluation trial was conducted from August 2004 to March 2005 in the laboratory of the National Institute of Malariolgy, Parasitology and Entomology, Ha Noi. Bioassays with An.dirus and Ae.aegypti (reared mosquitoes) were made according to the WHO method for evaluation of susceptibility and residual effects of the insecticide on the painted and nylon wall surface. Ale lose sprayed at the dose of 30mg ai/m2 were found to produce residual effect for 6-7 months on the painted wall surfaces (mosquito mortalities: 51.1%-67.8%) and for 4-5 months on nylon wall surface (mosquito mortality: 55.6%-67.8%).
Malaria ; Pyrethrins ; Laboratories

Through pH determination, acide- color quantification, extraction time and stabilization time of solvent extracting layer, optimal parameters were determined to quantify loperamide. The method could have high sensitive and selectivity with precise and repetitive results; its is appropriate for laboratories at low level of technique equipments
Loperamide ; Pharmaceutical Preparations ; Laboratories

The eggs of Anopheles dirus originated from China and currently colonized at the National Institute of Malariology, Parasitology and Entomology kept at 24 - 26°C on wet cotton for 20 - 25 days gave hatching rate approximately of 40%. The suitable food for Anopheles dirus larvae consisted of 6g bread powder, 2g green bean powder and 0.5mg vitamin B 1. The appropriate density for rearing An. dirus larvae is 0.4 larva per square centimetre of water surface
Malaria ; Anopheles ; Laboratories

120 normal subjects without bleeding history aged 20-77 were studied. Bleeding time 169 seconds, there is no significant statistic difference in genders and age groups. Plasma fibrinogen level was 2.56 g/l, no significant difference in 2 genders. In the under 45 years old group, fibrinogen level was lower than in above 45 yeas old group with statistical significant. No case of non-contracted blood clot occured, 2.5% of blood clot was contrated non completely, 97.5% completely. Alcohol test was negative in all cases. No case of fibrinolyse time < 90 minutes occured.
Hemostasis ; Blood Coagulation ; Laboratories

The Biomedical Laboratory Center of Hanoi Medical University was established in January 17th 1997 and comprises 4 small labors: the functional tests; biochemical; immunology and genetic. According to its functions and tasks, the labor has human resources with the high technical and scientific levels. This resource originated from the faculties of Hanoi Medical University. This is an activity pattern which is suitable and convenient for staffs and students in the university.
Technology Assessment, Biomedical ; Laboratories

The survey covered 11 cities/provinces with 179 servey copies distributed to staffs involved in microbiological testing during 2003-2004. Results showed that tested microbiological samples of water and food were 790±817 and 699± 552 (x±SD)/year, respectively, with 4-5 testing indicators applying for MPN7-9 tubes. The number of samples for testing microorganism in the environment was still limited. In five years of 2000-2004, epidemics occurred in some provinces like typhoid, cholera and dengue fever. Those were tested for causes making up 88% and the rate of success was high. Some causes for epidemics (typhoid, cholera, dengue fever and pacillary dysentery) can be identified at provincial centres of preventive health.
Preventive Medicine ; Laboratories

Clinical and laboratory findings was investigated on 46 patients who had diagnosis of lupus glomerulonephritis type IV. The obtained results showed that clinical features are severe with 45.7% patients had hypertension, 34.8% patients had acute nephritis syndrome and 56.5% had nephrotic syndrome. Especially, there were 95.7% patients have renal insufficiency. Among them, 26.1% had a severe renal failure (blood creatinine level >300mmol/I). But after 1 month of treatment, 100% of renal insufficiency patients have blood creatinine level < 300mmol/l and 72.8% of renal failure patients who had creatinine level > 300mmol/l had improvement in renal function
Lupus Nephritis, Laboratories, Diagnosis

Vnukovo-32 a cell culture rabies is used to study the vaccine strain technology of inactivated culture rabies vaccine production in primary hamster kidney cells. The master and working seeds meet the criteria of rabies vaccine strain titer (5.5 log LD 50 - 7.1 log LD 50/ml) according to WHO standard. During the study, we have used the procedure for inactivated, non concentrated rabies vaccine production of Russia and other countries procedures as references. The result was we constructed the procedure for inactivated, concentrated cell culture rabies vaccine production at laboratory scale.
Rabies Vaccines ; Cells, Laboratories