2.Database Conversion and Analysis of Antimicrobial Resistance Profile for Microscan using BacLink 2 Software.
Jae Seok KIM ; Young Kyung LEE ; Ji Young PARK ; Hee Jung KANG ; Sung Ha KANG ; Young Chul KIM ; Hyoun Chan CHO
Journal of Laboratory Medicine and Quality Assurance 2003;25(1):189-193
BACKGROUND: In hospital laboratory using Microscan, the search for an isolate or the analysis for antimicrobial susceptibility rates were obtained by the Data Management System (DMS) software. However, it is hard to convert DMS database to other file formats in addition to some limitation in using the database. We applied BacLink 2 and WHONET 5.1 softwares to convert and analyse DMS database for the utilization of the isolate profiles and the antimicrobial resistance rates. METHODS: Specimen and microbial data were printed as 'Short report form', an ASCII text file, from Microscan DMS. BacLink 2 software was used to convert the printed file to dBASE format file. Statistical analyses were performed using WHONET 5.1 software. RESULTS: Data of isolates were obtained as 'Short report form' in one month intervals. This file could be converted to other database file using BacLink 2 software. The antimicrobial resistant profiles were obtained, and the susceptibility, intermediate resistant, and resistant rates for each isolates could be analyzed. CONCLUSIONS: In this study, BacLink 2 and WHONET 5.1 software were successfully applied for the conversion of the database. Analysis of isolate profiles and antimicrobial resistant rates could be performed in other personal computer systems. The database management by BacLink 2 and WHONET 5.1 software could be applicable for the convenient statistical analysis in microbiology laboratories using Microscan.
Laboratories, Hospital
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Microcomputers
3.Quality control network in laboratories of Vietnam Inter’l hospital
Journal of Practical Medicine 2000;383(6):45-48
This study introduced the quality control network in laboratories of Vietnam Inter’l hospital, including internal and external quality control. The values of samples were at allowed limitations. The values of SD, CV what a high preciseness when comparing with these in other Inter’l Laboratories
Quality Control
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Laboratories
4.The biomedical laboratory center
Journal of Medical Research 2001;15(2):50-52
The Biomedical Laboratory Center of Hanoi Medical University was established in January 17th 1997 and comprises 4 small labors: the functional tests; biochemical; immunology and genetic. According to its functions and tasks, the labor has human resources with the high technical and scientific levels. This resource originated from the faculties of Hanoi Medical University. This is an activity pattern which is suitable and convenient for staffs and students in the university.
Technology Assessment, Biomedical
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Laboratories
5.Microorganic laboratory testing service at provincial centres of preventive medicine
Journal of Preventive Medicine 2005;15(4):5-10
The survey covered 11 cities/provinces with 179 servey copies distributed to staffs involved in microbiological testing during 2003-2004. Results showed that tested microbiological samples of water and food were 790±817 and 699± 552 (x±SD)/year, respectively, with 4-5 testing indicators applying for MPN7-9 tubes. The number of samples for testing microorganism in the environment was still limited. In five years of 2000-2004, epidemics occurred in some provinces like typhoid, cholera and dengue fever. Those were tested for causes making up 88% and the rate of success was high. Some causes for epidemics (typhoid, cholera, dengue fever and pacillary dysentery) can be identified at provincial centres of preventive health.
Preventive Medicine
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Laboratories
6.Clinical and laboratory findings of type IV lupus glomerulonephritis
Journal of Medical Research 2005;34(2):39-44
Clinical and laboratory findings was investigated on 46 patients who had diagnosis of lupus glomerulonephritis type IV. The obtained results showed that clinical features are severe with 45.7% patients had hypertension, 34.8% patients had acute nephritis syndrome and 56.5% had nephrotic syndrome. Especially, there were 95.7% patients have renal insufficiency. Among them, 26.1% had a severe renal failure (blood creatinine level >300mmol/I). But after 1 month of treatment, 100% of renal insufficiency patients have blood creatinine level < 300mmol/l and 72.8% of renal failure patients who had creatinine level > 300mmol/l had improvement in renal function
Lupus Nephritis, Laboratories, Diagnosis
7.Study on the technology to produce inactivated rabies vaccine on the primary hamster kidney cell at laboratory scale
Journal of Preventive Medicine 2005;15(5):123-127
Vnukovo-32 a cell culture rabies is used to study the vaccine strain technology of inactivated culture rabies vaccine production in primary hamster kidney cells. The master and working seeds meet the criteria of rabies vaccine strain titer (5.5 log LD 50 - 7.1 log LD 50/ml) according to WHO standard. During the study, we have used the procedure for inactivated, non concentrated rabies vaccine production of Russia and other countries procedures as references. The result was we constructed the procedure for inactivated, concentrated cell culture rabies vaccine production at laboratory scale.
Rabies Vaccines
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Cells, Laboratories
8.Social investigation on laboratory activities in provincial centres for preventive medicine
Journal of Preventive Medicine 2005;15(5):150-155
An analysis was conducted on 187 surveyed records of social investigation on laboratory activities at Centres for preventive medicine of 11 provinces from 2003 to 2004. The investigation revealed that 99% of interviewees said that the laboratory activities were very necessary, 78% of them said that the test results were reliable, and 74% of them assumed that their lab skills are rather good. We suppose that it is necessary to improve basic salary with their skilled jobs. The budget allocation should be reviewed for laboratory activities, and procurement of equipment and biomedicals and chemicals.
Preventive Medicine
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Laboratories
9.Preliminary dat on the colonizing of An.sundaicus in the laboratory
Journal of Malaria and parasite diseases Control 2003;3():79-84
Freshly fed or half gravid An.sundaicus collected in An Thoi Dong commune, Can Gio district, Ho Chi Minh city were brought to the insectary of NIMPE for colonizing. Larvae were fed by a combination of 4gr bread powder + 4 shrimp powder + 2gr bean powder + 0.001gr vitamine B1. Larvae were contained at the conditions: a density of 0.3 larvae/cm2 of the surface water. Water for rearing was taken form the field with the salinity of 0.6 – 0.8%. Room temperature of 27 – 31oC, humidity of 60 – 90%. At these conditions, the image period took the time of 5 – 17 days. From the 8th generation (F8), mosquitoes were found to take free matting in the cage of 30 x 30 x 30 cm
Laboratories
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Culicidae
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diagnosis
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Diptera
10.Establishment of a stenogamous colony of Anopheles minimus theobald, 1901 in the laboratory
Journal of Malaria and parasite diseases Control 2003;0(6):48-52
An. minimus was collected from Hoa Son village, Luong Son district, Hoa Binh province in November 1997 and maintained in the laboratory of NIMPE by artificial mating techniques. Since 51st generation, a free mating colony in the small cages (with dimensions of 30 x 30 x 30cm) has been established by releasing 200 male and 200 female mosquitoes per cage. The releasing of additional 50 males of An. minimus to the cage has been found to stimulate the mating process in small cage
Anopheles
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Laboratories
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Mosquito Control