Primary aldosteronism(PA) is one of the most common causes of secondary hypertension,plasma aldosterone concentration(PAC)/plasma renin activity (PRA) ratio is widely used in clinical practice.However,PRA reflects the level of plasma renin indirectly.In recent years,plasma renin concentration (PRC) has been measured by automatic chemiluminescence immunoassay,which is more stable,convenient and with less confounding factors than conventional renin activity.This review briefly introduces methods of examining PRA,PRC and PAC,and compares the value of PAC/PRC ratio with PAC/PRA ratio in PA detection.

Objective To study the role of gene Fas and FasL expression in apoptosis of plcenta with ICP. Methods TUNEL (TdT-mediated dUTP nick end labeling) and immuno-hisochemistry methods were used for 31 placental samples with ICP and 31 normal placental samples to detect apoptosis index (AI) and the expression of Fas and FasL in placental tissues. Results AI of cytotrophoblast, syncytotrophoblast, decidual cells and mediate cell(49.09?9.13,t=13.41;46.64?9.77,t=12.16;35.09?9.49,t=8.43;38.74?9.70,t=11.28) in ICP group was significantly higher than that of control group (P

Objective:The effect of Tonglin capsule was observed on type steblay interstitial nephritis of cavy.Methods: The cavy was immunized using the basement membrane of renal tubule from renal cortex of rabbit and Freund's complete adjuvant (FCA). The animal model of autoimmunity renal tubule interstitial nephrifis was made, The effect of Tonglin capsule on nephric pathological changes was observed. Results: ① The levels of creatinine (Cr) and blood uria nitrogen (BUN) in treatment groups were obviously lower than that in model group ( P

Objective To study the incidence of complications of visceral and vascular injury in laparoscopic surgery for gynecologic diseases,and to discuss the ways to decrease the incidence. Methods The data of 2684 patients who received laparoscopic surgery from Januray 2003 to December 2005 in the Department of Obstetrics and Gynecology were reviewed retrospectively.The incidence and treatment of complications of visceral and vascular injury were observed. Results The total incidence rate of complications was 2.53%(n=68),and that of the visceral and vascular injury was 0.37%(n=10).Four cases of injury were related with trocar punctures(injury of omental blood vessel,n=2;postperitoneal vessel,n=2),three took place during the operation (severe bleeding,n=1;bladder injury,n=2),and the other three were observed in the postoperative stage(ureter injury,n=2;intestinal fistula,n=1). Conclusion The complications in laparoscopic surgery for gynecologic diseases are increased with the extension and difficulty of operation,and are closely related with the experience of the surgeons.Proper candidates for the surgery and established operative technique are the key factors in decreasing the incidence.

ObjectiveTo observe the preventive effect of type Ⅱ collagen on experimental rat articular cartilage damage induced by T-2 toxin,to explore molecular biomarkers of articular cartilage damage and repair,and to provide a theoretical basis for control of articular cartilage damage.MethodsEighty Wistar rats were randomly divided into 4 groups according to their body weights:negative control,positive control,high-dose intervention,and low-dose intervention groups,20 rats in each group.Animals in negative control group were fed with standard rat chow,and animals in other three groups were fed with T-2-toxin-contaminated chow( 100 ng/kgfeed).Animals in negative and positive control groups drank distilled water,animals in high-dose intervention and low-dose intervention groups drank water containing type Ⅱ collagen(0.5,5.0 g/L,respectively).These rats were sacrificed after 3 and 5 months,respectively,and bilateral knee joints were collected.Histopathologic changes in hyaline cartilage were examined by light microscope,serum levels of type Ⅱ collagen carboxyl terminal peptide (CTX-Ⅱ ),cartilage oligomeric matrix protein (COMP) and urinary deoxypyridinoline (DPD) were determined by enzyme-linked immunosorbent assay(ELISA).ResultsHE staining showed,that the positive control articular chondrocytes were disarranged,deformated,degenerated,with necrosis and extensive areas of chondrocyte loss;but the two intervention groups only showed fibril formation and swelling and surface cartilage cells became round,flat cartilage cells decreased in number,and cartilage cells clustered and so on early pathological changes of osteoarthritis.At the ends of 3 month and 5 month experiment,the levels of serum CTX- Ⅱ in different groups were,negative control[(18.77 ± 4.61),(25.07 ± 9.17)μg/L],high-dose intervention[ (21.11 ± 5.02),(33.20 ± 9.74)μg/L ],low-dose intervention [ ( 19.87 ± 4.53 ),( 29.73 ± 9.32 ) μg/L ] and positive control [ ( 24.43 ± 5.23 ),( 39.17 ±10.49 ) μg/L ] ; the levels of serum COMP were,negative control group [ (5.43 ± 2.75 ),( 6.38 ± 2.23 ) μg/L ],highdose intervention group[ (17.27 ± 4.77),(20.32 ± 4.74)μg/L],low-dose intervention group[(20.13 ± 5.07),(19.44 ± 4.92)μg/L] and positive control group[ (21.37 ± 4.72),(24.52 ± 4.26)μg/L].At the end of 3 month,compared with negative control group,the level of serum CTX- Ⅱ in other three groups increased,but only positive control group increased significantly(P ＜ 0.05) ; at the end of 5 month,compared with negative control group,the level of serum CTX-Ⅱ in other three groups increased significantly,and the difference was statistically significant (all P ＜ 0.05),and the level of CTX-Ⅱ in the two intervention groups was significantly lower compared with that of positive control group(all P ＜ 0.05).Compared with negative control group,the level of serum COMP in other groups increased significantly at the end of 3 month (all P ＜ 0.05) and only the level of serum COMP in high-dose intervention group was significantly lower compared with that of positive control group(P ＜ 0.05).At the end of 5 month,compared with negative control group,the level of serum COMP in other three groups increased significantly,the difference were statistically significant (all P ＜ 0.05) ; the levels of serum COMP in the two intervention groups were significantly lower than that of positive control group(all P ＜ 0.05).At the ends of 3 month and 5 month,the content of urinary DPD in negative control group were[ (3.47 ± 2.20),(4.14 ± 1.06)μg/L],positive control group[ (4.09 ± 2.48),(4.33 ± 3.43)μg/L],high-dose intervention group[ (3.86 ± 2.31 ),(5.72 ± 3.89)μg/L] and low-dose intervention group[ (3.58 ± 2.77),(4.23 ± 2.90)μg/L].The difference between the 4 groups were not statistically significant (F =2.608,2.436,all P ＞ 0.05).ConclusionsType Ⅱ collagen could effectively reduce the level of serum CTX-Ⅱ and COMP in experimental rats and delay the process of articular cartilage damage induced by T-2 toxin.

ObjectiveTo study the impact of jogging mode on T-2 toxin-induced articular cartilage injury in rats,and to evaluate the role of movement in the development of bone and joint disease.MethodsA hundred Wistar rats were randomly divided into five groups:negative control group(free activities in the cage),positive control group(firee activities in the cage),high-regulation group(regular exercise,the treadmill speed of 24 m/min),lowregulation group (regular exercise,the treadmill speed of 12 m/min) and the random group(random exercise,the treadmill speed of 12 or 24 n/min).The negative control group was fed on commercial grain fodder and other groups were fed on grain fodder contaminated with T-2 toxin.At the end of 5,10 weeks,the histopathological changes of hyaline cartilage were detected by optical microscope,and the level of serum cartilage oligomeric matrix protein (COMP) was determined.ResultsArticular cartilage lesions in each experimental group was evident,presented as cartilage cell degeneration,necrosis,karyopyknosis deeply stained,cells arranged in disorder and cell proliferation,articular dryness,and so on.Compared with the positive control group,the cartilage surface cells of rats in the movement groups showed degeneration,necrosis and loss of cells obviously.The injury in high-regulation group was the most serious than that in other movement groups,with the surface and the middle layer lesions,and a large area of cartilage necrosis,and loss of matrix collagen; cartilage degeneration,polarity disappeared,cell proliferation-based disorder showed in random group.The pathological changes of rat articular cartilage damage worsened with the extension of experimental period.The serum levels of COMP at week 5 in experimental groups were higher than that of both the negative control group and the positive control group,and the difference was statistically significant (F =15.733,P ＜ 0.05 ); compared with negative control group [ (11.55 ± 0.89)μg/L],the COMP levels in high-regulation group,low-regulation group,random group[(13.95 ± 1.23),(14.96 ± 1.29),( 12.99 ± 1.43)μg/L] were significantly higher(all P ＜ 0.05); compared with the positive control group[(12.32 ± 1.38) μg/L],the COMP levels in high-regulation group and low-regulation group were significantly higher(all P ＜ 0.05) ; and compared between the exercise groups,the COMP levels in low-regulatinn group were higher than that of random group(P ＜ 0.05).At week 10,the changes were in the same trend as that of week 5,and the difference between groups was statistically significant (F =6.144,P ＜ 0.05) ; and compared with the negative control group [(10.59 ± 1.93)μg/L],the COMP levels in high-regulation group,low-regulation group,random group [ ( 13.72 ± 2.67 ),( 14.94 ± 1.06 ),( 13.21 ± 1.58 ) μg/L] were significantly higher(all P ＜ 0.05) ; compared with the positive control group[ (11.45 ± 0.12)μg/L],the COMP levels in low-regulation group were significantly higher (P＜0.05); but compared with the exercise groups,the difference were not statistically significant(all P＞0.05).ConclusionsHigh-intensity regular running and irregular intensity running can increase the articular cartilage damage,and injury of articular cartilage by low-intensity treadmill exercise is not significant.

BACKGROUND: Chinese traditional osteopathy is long in history, unique in manipulation and miraculous in therapeutic effect. But people understand it more m perception rather than in theory, more in application rather than in development. There is little research truly on the bioseience.OBJECTIVE: To probe into the macro-idea of Chinese traditional osteopathy, micro-mechanisms on characters and mathematics-physics models, aiming to provide new principles and approaches of treatment for the daily increased bone trauma, fracture and sport injury.SETTING: Physics and machine-electron college of a university, and its affiliated hospital.METHODS: Based on the natural concept of "integration between heaven and human being" and new concept of holistic medicine in Chinese traditional osteopathy, the macro-idea and characters of reduction and union of fracture are generalized from the characters of natural therapy and the biomechanical mechanisms and characters of reduction and union of fracture are summarized from the micro-reaction of bone repair and union so as to discover biomechanical mechanisms and characters of reduction and union of fracture and further to set up biomechanical models and mathematics-physics expressions during the treatment.RESULTS: Chinese traditional osteopathy envelopes macro-idea of "initiative reduction-functional union" in fracture and micro-mechanism on "stress adaptability-functional adaptability" of bone repair and union.CONCLUSION: Chinese traditional osteopathy compiles with the natural,green and non-traumatic therapy in bio-natural law of bone repair and union and supports the theme of "high thought and high skill".

Objective: To investigate the effect of protein ki nase C on signal transduction such as tyrosine phosphorylation, c-fos and c-ju n mRNA expression in antigen activated mast cells. Methods: RBL-2H3 cells either untreated or treated with phorbol 12-myristate 13 -acetate (PMA) were sensitized with anti-DNP IgE, and activated with DNP-BSA, histamine release and tyrosine phosphorylation were quantitatively measured by ELISA and flow cytometry, respectively. The effect of PKC on the ex pression of c-fos and c-jun in serum-deprived RBL-2H3 cells activated by DNP-BSA detected by ethidium staining of PCR-amplified cDNA, the amplified cDNA products were subjected to Southern blot hybridization using specific prob es to determine the veracity of amplification. Results: Tyr osine phosphorylation and histamine release were significantly reduced from (4.4 7±0.03)% to (2.79±0.07)% and (104.47±1.31) nmol/L to (60.75±1.38) nm ol/L, respectively, 45 min after DNP-BSA stimulation in sensitized cells pre treated with PMA for 48 h. Bands of the size predicted for the amplified cDNA we re obtained: 299 bp for c-fos, and 651 bp for c-jun, a decrease of 91% and 82% , respectively, for c-fos and c-jun mRNAs was observed in antigen stimulated c ells pretreated with PMA for 48 h. Conclusion: PKC plays an impo rtant role in modulating the tyrosine phosphorylation and histamine release resp onses and may upregulate the expression of c-fos and c-jun in antigen activate d mast cell.

The short report will be focused on the genetic basis and possible mechanisms of tumorigenesis, common types of cancer, the importance of genetic diagnosis of cancer, and the methodology of cancer genetic diagnosis. They will also review presymptomatic testing of hereditary cancers, and the application of expression profiling to identify patients likely to benefit from particular therapeutic approaches.
Genes, Tumor Suppressor ; Genetic Techniques ; Genetics, Medical ; Humans ; Neoplasms ; diagnosis ; genetics ; Nucleic Acid Hybridization ; Sequence Analysis, DNA