1.Different Telomere Fragments Promote Rat's Vascular Smooth Muscle Cell Apoptosis by Silent Information Regulator 1/Cancer Suppressor Gene P53
Chinese Circulation Journal 2017;32(6):612-616
Objective: To study the influence of different telomere oligonucleotide on rat's thoracic aortic smooth muscle (A7R5 ) cell apoptosis and to clarify weather silent information regulator 1 (SIRT1)/cancer suppressor gene (P53) signaling pathway involved in oligonucleotide induced cell apoptosis. Methods: 3 telomere repeat sequences of TE-12 (TTAGGG)2, TE-24 (TTAGGG)4 and TE-36 (TTAGGG)6 were respectively transfected into rat's A7R5 cells as TE-12 group, TE-24 group and TE-36 group; in addition, blank A7R5 cells were used as Control group. Transfection induced A7R5 cell apoptosis was detected by flow cytometry, mRNA and protein expressions of SIRT1 and P53 in A7R5 cells were examined by RT-PCR and Western blot analysis in different groups. Results: Successful ransfection rates were similar among 3 groups. Compared with Control group and TE-36, TE-24 groups, the highest apoptosis rate of A7R5 cells was found in TE-12 group and the lowest was found in TE-24 group which was still higher than that in Control group, allP<0.05. mRNA and protein expressions of SIRT1 was obviously higher in TE-12 group than the other 3 groups, allP<0.05; mRNA and protein expressions of P53 was obviously lower in TE-24 group than the other 3 groups, allP<0.05. Conclusion: Telomere oligonucleotide sequence may promote rat's A7R5 cell apoptosis; different sequence had various influence and the strongest effect was observed in TE-12 sequence. The above impact might be related to SIRT1/P53 signaling pathway.
2.Evaluation of therapeutic studies on chemotherapy and/or radiotherapy-induced oral mucositis published in Chinese journals by means of evidence-based medicine
Na LV ; Juan XIA ; Chunyang LI
Journal of Practical Stomatology 1996;0(02):-
Objective:To evaluate the quality and the effectiveness of therapeutic studies on chemotherapy and/or radiotherapy-induced oral mucositis published in Chinese journals by means of evidence-based medicine. Methods:Relevant papers were searched in Chinese Biological Medical Disc (CBMDisc)database and China National Knowledge Infrastructure(CNKI) database. Papers using randomized controlled trials (RCT) were identified and analyzed according to the international standards of evidence-based medicine. Results:There were totally 98 articles focusing on therapeutic studies of chemotherapy and/or radiotherapy-induced oral mucositis and only 22 of them met RCT criteria. Study design, diagnostic standards, therapeutic effects and adverse effects decribed in those 22 papers were fully evaluated by means of evidence-based medicine. Conclusions:Research quality of the analyzed papers could not meet clinical needs and Meta analysis could not been done due to their low quantity and poor quality. However, as far as the published articles concerned, GM-CSF showed a good short-term treatment effect.
3.Senegenin Protects against Ischemia-reperfusion Inj ury by Ameliorating Endoplasmic Reticulum Stress in Mice
Zixi LI ; Juan CHEN ; Jianxin LV
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong 2014;(4):409-412
Objective To investigate the protective role of senegenin(SEN)in the mouse ischemia-reperfusion injury and its mechanism.Methods Mice were divided at random into control group(the ligature was placed but not ligated for 225 min),is-chemia-reperfusion model group(the anterior descending coronary artery remained ligated for 45 min and then reperfused for 180 min)and SEN treatment group(30 mg/kg SEN was used 10 min before reperfusion).The myocardial infarct size was meas-ured by using Evans blue-TTC staining.Fluorescence assay was employed to detect the activity of Caspase-1 2 and Caspase-3 to assess the myocardial apoptosis.Western blot was used to detect the expression of two endoplasmic reticulum stress (ERS) markers,GRP78 and CHOP,in infarcted myocardia.Results Compared with the control group,the myocardial infarct size was significantly increased,the activities of Caspase-12 and Caspase-3 were increased by 4.71 fold and 3.37 fold,respectively,and the expression levels of ERS markers GRP78 and CHOP were conspicuously elevated in the ischemia-reperfusion model group.Pretreatment with SEN(30 mg/kg)could significantly reduce the myocardial infarct size,the activities of Caspase-12 and Caspase-3 and the expression levels of GRP78 and CHOP when compared with those in the ischemia-reperfusion model group and there was significant difference between the two groups(P<0.05 or P<0.01).Conclusion SEN can protect against the myocardial ischemia-reperfusion inj ury by ameliorating ERS-mediated myocardial apoptosis in mice.
4.One case report of type Ⅲ of dens in dente of maxillary bilateral incisors
Ruzhou FENG ; Changhai LV ; Juan LIU
Journal of Practical Stomatology 2015;(6):873-874
Dens in dente is a rare malformation of teeth.This article reports one case of type Ⅲ dens in dente of maxillary bilateral incisors with acute periapical lesion.The case was treated successfully by apexification with Vitapex paste.
5.Change of activity of serum paraoxonase in patients with acute organophosphorus poisoning.
Juan-wen ZHANG ; Guo-cai LV ; Yu-qin JIN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(10):610-611
Acute Disease
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Adult
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Aged
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Aryldialkylphosphatase
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blood
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Female
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Humans
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Male
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Middle Aged
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Organophosphate Poisoning
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Young Adult
6.Significance of determination of serum xanthine oxidase and lipid peroxidation indexes in acute organophosphorus poisoning.
Juan-wen ZHANG ; Guo-cai LV ; Yu-qin JIN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(4):239-240
Acute Disease
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Adult
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Aged
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Female
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Humans
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Lipid Peroxidation
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Male
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Malondialdehyde
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blood
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Middle Aged
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Organophosphate Poisoning
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Oxidative Stress
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Superoxide Dismutase
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blood
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Xanthine Oxidase
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blood
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Young Adult
8.Research of anti-keratin monocional antibody on animal's psoriasis
Xiangping HU ; Juan LIU ; Zhaoyong AN ; Shaomin LV ; Fang CHEN
Journal of Chinese Physician 2008;10(5):615-617
Objective In order to explore the mechanisms of the anti-keratin monoclonal antibody 5G5(MAbSG5)in psoriasis treatment.Methods The different groups of mouse were fed with 5G5.The effect on regulating keratinocyte proliferation was observed.Results The experimental study revealed that MAb5G5 has the function of restraining the karyokinesis of vagina epithelium and accelerating the formation of stratum granulosum in rat squamous epidermis.Conclusion The mechanism of MAb5G5 in psoriagis treatment may be related to regulate keratinocyte proliferation.
9.Expression and clinical significance of serum chemokines in patients with paraquat poisoning.
Juan-Wen ZHANG ; Jun-Ling HE ; Guo-Cai LV
Chinese Journal of Industrial Hygiene and Occupational Diseases 2009;27(6):369-370
Acute Disease
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Adult
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Aged
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Chemokines
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blood
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Female
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Humans
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Male
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Middle Aged
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Paraquat
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poisoning
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Prognosis
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Young Adult
10.Reasonable analysis of falls of female inpatients in psychotics department and nursing methods
Liuliu XU ; Wenjun LV ; Juan DU ; Ping CHEN ; Xiaohui MA
Modern Clinical Nursing 2015;14(10):43-46
Objective To analyze the falls of female inpatients with the psychotics diseases and summarize effective nursing intervention. Method Two hundred and eighty-nine psychiatric female inpatients from April 2014 to August 2014 were collected, and the reasons of falls cases during this period were analyzed and nursing methods were formulated. Result Nine patients fell, with an incidence of 3.11%(9/289) including the reason of lack of risk dynamic evaluation, lack of fall prevention measures and shortage of nursing personnel. Conclusions The reasons for the falls of the hospitalized psychiatric female patients include lack of risk dynamic evaluation, lack of fall prevention measures, shortage of nursing personnel. The nursing countermeasures include risk assessment of dynamic management and the implementation of the fall prevention measures , strengthening training and health education so as to prevent and reduce the occurrence of falls and ensure patients'safety.