Objective To establish a special determination method of astragaloside Ⅳin Xiyangshen Jinqiangui Mixture by HLPC-ELSD. Methods HPLC-ELSD was performed on Inertsil C18 Column(5 ?m,4.6 mm?250 mm),acetonitrile-water(30 ∶70) served as mobile phase ,column temperature was 35 ℃,the flow rate was 1.0 mL?min-1,and an evaporating light-scattering detector (ELSD) was used as detector. Results For astragaloside Ⅳ,the linear range was 0.424 4～2.122 ?g and the average recovery was 101.77 %with corresponding RSD being 2.49 %(n=6). Conclusion The method is simple,sensitive ,reproducible,accurate and can be used for the quality control of Xiyangshen Jinqiangui Mixture.

In our experiment it was found thatinsulin was able to significantlyenhance the response of the thymo-cytes treated with nylon wool toConA in the presence of exogenousIL-1.It had been demonstrated thatinsulin was able to augment the pro-duction of IL-1 by macrophagesstimulated by LPS.These resultssuggest that the regulation of insulinon T cell activiation be associatedwith macrophages or IL-1 andinsulin play a regulating rolein other reactions mediated withIL-1.In addition,it was alsodemonstrated that the action of activeinsulin could be weakened if inac-tive insulin be not removed.

Objective To observe the long-term efficacy of nasal endoscopy-guided high intensity focused ultrasound (HIFU ) in the treatment of allergic rhinitis .Methods 300 patients with allergic rhinitis who failed in medication were randomly divided into the treatment group(n=162) and the control group(n=138) .The patients in the treatment group were accepted the nasal endosco-py-guided HIFU therapy ,while the patients in the control group were subjected to the hypothermy plasma ablation .The two groups were followed up after operation ,and the visual analogue scale(VAS) was employed to evaluate the therapeutic efficacy at postoper-ative 3 ,6 ,12 ,24 months .The saccharin tests were performed at the beginning and ending of treatment to assess the nasal mucocili-ary function in the patients of the two groups who voluntarily accepted the functional test of nasal cilia .Results After 2-week treat-ment ,nasal congestion ,sneezing ,runny nose ,nasal itching and other symptoms in the two groups were significantly relieved with no obvious complication .2-year follow-up demonstrated that the total effective rate in the treatment group was 61 .4% ,which was markedly higher than 50 .7% in the control group(P=0 .017) .The average saccharin clearance time of the treatment group (n=49) and the control group(n=39) were(464 ± 152)s and(738 ± 149)s ,respectively ,and their difference was statistical significant (P=0 .026) .Conclusion The long-term efficacy of HIFU treatment in allergic rhinitis is superior to that of hypothermy plasma abla-tion ,possesses less impacts on the nasal mucociliary function ,and is worthy of clinical promotion .

Objective To evaluate the clinical intubation of Bonfils intubation fiberscope in difficult airway of acromegaly patients.Methods Fifteen acromegaly patients who have one of the following criteria of preoperative airway assessment,Mallampati score ≥3,thyromental distance (≤6 cm),mouth opening(≤3.5 cm).After routine anesthetic induction the patients were intubated with Bonfils intubation fiberscope.Haemodynamic changes were observed and handling of the Bonfils intubation fiberscope was evaluated in terms of the ease of insertion into oropharynx,visualization of epiglottis,advancement into glottis aperture and slide down the tracheal tube.Intubating time and success rate were also recorded.Postoperatively,sore throat or hoarseness were followed up for all patients.Results After intubation systolic pressure and heart rate increased as compared with pre-intubation((P

OBJECTIVE:To observe the effect of acyclovir on neurological function and cytokines of children with viral menin-gitis. METHODS:Totally 70 children with viral meningitis were randomly divided into control group and observation group. All children were given routine treatment,including defervescence,reducing intracranial pressure and regulating water and electrolyte balance,etc. Based on it,the control group was treated by Ribavirin glucose injection 15 mg/kg,iv,bid;observation group was treated by Acyclovir glucose injection 5 mg/kg,iv,tid. The course for both was 7 d. The clinical data was compared,including the vascular endothelial growth factor (VEGF),matrix metalloproteinase-9 (MMP-9) in cerebrospinal fluid (CSF) and serum,insu-lin-like growth factor-Ⅱ(IGF-Ⅱ) and insulin like growth factor binding protein-3(IGFBP-3) in CSF before and after treatment and the incidence of adverse reactions. There were no obvious adverse reactions during the treatment. RESULTS:After treatment, the VEGF and MMP-9 in serum and the VEGF,MMP-9,IGF-Ⅱ and IGFBP-3 in CSF in 2 groups were significantly lower than before,and observation group was lower than control group,with significant differences(P<0.05). There was no adverse reactions in 2 groups during the treatment. CONCLUSIONS:Compared with ribavirin,acyclovir can more obviously improve the neurological function and cytokines of children with viral meningitis,with similar safety.

0.05). Compared with controlled central venous pressure (CVP) before and right after hepatectomy, CVP increased significantly (P

Objective To investigate serum procalcitonin(PCT)and C-reactive protein(CRP)and clinical significance of changes in children with pneumonia.Methods 123 cases of childhood pneumonia included 48 cases of bacterial infection,mycoplasma infection in 36 cases,39 cases with viral infections,40 cases of physical healthy children were selected as the control group.They were measured in serum PCT and CRP content of the correlation between test results and analysis of clinical final diagnosis.Results The level of PCT in Bacterial pneumonia group, Mycoplasma pneumonia group and viral pneumonia group were (5.80 ±1.92)μg/L,(0.45 ±0.15 )μg/L and (0.32 ±0.17)μg/L,respectively,significantly higher than control group(0.19 ±0.10)μg/L(t =5.37,3.41,1.23, all P <0.05);the level of CRP in Bacterial pneumonia group,mycoplasma pneumonia group and viral pneumonia group were(45.34 ±13.35 )mg/L,(28.63 ±11.37)mg/L and (8.19 ±2.07)mg/L,respectively,significantly higher than control group(3.85 ±1.31)mg/L(t =5.37,3.41,1.23,all P <0.05);serum PCT sensitivity to detect pneumonia children,specificity and the positive predictive were 93.56%,82.35% and 69.25%,respectively,higher than serum CRP diagnosis of pneumonia sensitivity,specificity and positive predictive(χ2 =4.41,5.83 and 7.62, respectively,all P <0.05).Conclusion Serum PCT and CRP measurement can be used as an auxiliary to identify indicators of childhood pneumonia,the diagnosis of pneumonia in children has important clinical significance.

Objective To investigate the expression of VCAM-1 and E-selectin in vessel walls of the patients with thromboangiitis obliterans(TAO).Methods The vessel tissues were taken from 18 cases of amputation operation due to TAO and 16 cases of amputation operation due to non-vascular diseases.The expression of VCAM-1 and E-selectin were assessed by the immunohistochemical method.Results The positive expression rate of VCAM-1 in vessel wall of the patients with TAO was 77.8%(14/18),which of E-selectin was 72.2%(13/18);both of them were obviously higher than 6.3%(1/16) and 6.3%(1/16) in the control group.The positive rate of VCAM-1 and E-selectin had no correlation with the age,smoking amount,smoking time and drug use (P>0.05).Conclusion The expression of VCAM-1 and E-selectin have close correlation with the occurrence and development of TAO.Detecting the expression of VCAM-1 and E-selectin can serve as a diagnostic indicator of TAO.

The clinical data of 26 cases with pathologically confirmed primary gastrointestinal nonHodgkin's lymphoma (PGINHL) admitted to our hospital from January 2001 to December 2010 were retrospectively analyzed.Among 26 patients,12 cases occurred in stomach,9 cases in small intestine,4 cases in colon and 1 case in multiple sites.The most common symptoms were abdominal pain (73％),followed by gastrointestinal bleeding (23％) and abdominal mass (15％).The main pathological types were MALT lymphoma (35％),diffuse large B-cell lymphoma (35％),and enteropathy-type T-cell lymphoma ( 15％ ).There were 14 patients in stage Ⅰ,6 in stage Ⅱ,1 in stage Ⅲ and 5 in stage ⅣV.Radical surgery and/or chemotherapy was the main therapy for PGINHL.

The Componential Framework of Creativity,advanced by Amabile,one of significant figures on the social psychology of creativity,comprehensively interpreted the nature and the construct components of creativity.Inspiring by the ideology of Amabile's Componential Framework of Creativity,this study examined whether the science research human resource management practices in today's Chinese science research institutions follow the nature of science research and whether the policy and operation inhibit creativity.It identified the common misconducts happened in human resource selection,development and performance management,and the causes and countermeasures of those misconducts were also explored.The findings will be a source of reference for human resource management practitioners at the science research field of China.