Objective To explore the clinical efficacy of theophylline retard-20 tablet in pediatric asthma treatment.Methods From April 2010 to December 2014 in our hospital 136 cases of children with infantile asthma were selected and divided into observation group and control group.Patients in the observation group were treated with sulfate aerosol inhalation therapy,patients in the control group were given oral theophylline retard-20 tablet treatment.After treatment,the treatment results were analyzed.Results In the observation group,functional recovery was found in 37 cases,significant progress in 20 cases,on effect in 11 cases,the total effective rate was 83.82％;Adverse reactions such as pharyngeal discomfort was found in 3 cases,upset stomach in 0 cases,heartburn in 0 cases,the incidence was 4.41％.In the control group,68 functional recovery was found in 19 cases,and remarkable progress in 26 cases,on effect in 23 cases,the total effective rate was 66.18％;Adverse reactions such as pharyngeal discomfort occurred in,4 cases,upset stomach in 3 case,heartburn in 4 cases,the incidence was 14.71％.The effect effective treatment was better than that of control group (P ＜ 0.05),the incidence of adverse reactions in observation group was significantly lower than the control group (P ＜ 0.05).ConcltSior The treatment of infantile asthma with salbutamol sulfate aerosol therapy is very effective and has,clinical significance,is worth further promotion and application.

Objective To establish an HPLC method for the simultaneous content determination of quercetin and kaempferol in Penthorum Chinense Pursh. Methods The HPLC analysis was carried out on Kromasil C18 (250 mm× 4.0 mm, 5.0 μm) with a mixture of methanol-0.1% phosphate (50:50) as the mobile phase. The determination wavelength was set at 360 nm with the flow rate of 1.0 mL/min. The column temperature was set at room temperature. Results The quercetin and kaempferol showed good linearity in the range of 0.105 6–2.112 0 μg and 0.023 6–0.472 μg respectively. The average recovery rates of quercetin and kaempferol were 98.46% (RSD=2.29%) and 98.17%(RSD=1.99%) respectively. Conclusion The method is accurate and sensitive, which can be used for the content determination of quercetin and kaempferol in Penthorum Chinense Pursh.

Quantum dots( QDs) as a new type of nanostructured luminescent materials have been widely used in biology, materials science, and physical optics. QD-based biosensors can rapidly and accurately detect biological macromolecules or inorganic molecules both in vivo and in vitro. The surface of QDs, prior to their biological detection applications, needed to be modified to enhance their fluorescence properties and lower their biological toxicity. Currently, the surface modification technologies mainly include polymer modification, thiol compounds modification, mercapto propionic acid compounds modification and organic groups modification. Various biosensors can be developed by adopting different modification techniques, which have been adopted to locate and track a variety of biological molecules in vivo. Although a large number of literatures have reported the biological applications of QD-based biosensors, rare systematic reviews of surface modification technologies on QDs have been witnessed. In this paper, we reviewed the surface modification technologies of QDs in biosensors and their medical applications.

Klebsiella Pneumoniae is an important pathogen for various infections clinically,in which hypervirulent Klebsiella pneumoniae (hvKP) mainly causes pyogenic liver abscess.By now,there are no standardized method to identify hvKP strains.hvKP strains are usually with hypermucoviscous phenotype,and the prevailing serotypes and clonal types are K1,K2 and CC23,CC65,respectively.Genes rmpA,magA,alls and kfu contribute to the pathogenicity of hvKP.This paper reviews the identification,clonal types and serotypes,hypermucoviscous phenotype and some other virulent genes of hvKP.

The bookmade precise discussions on syndrome differentiation and treatment of infantile malnutrition.This article tried to explore the diagnostic and therapeutic features of infantile malnutrition from four aspects:etiopathogenisis and location of disease:classificafion of therapeutical principle;andinternal and external therapy.

Objective Through discussing application of case analysis to improve the comprehensive quality of nursing staff,and promote their implementation of high quality nursing care for the patients.Methods all 61 nurses in general wards were trained by daily half-hour case analysis method.After the training,questionnaire was given to investigate the nursing staff's evaluation on case analysis training,and at the same time compared before and after the training,the patients' satisfaction survey result,the monthly examination result of the nursing department,nursing paper quality and the number of nursing adverse events.The relevant data was done by t test orx2 test.Results After the implementation for half an hour case analysis training,most nurses think their own various ability was improved.After the training,the patient satisfaction was (t=-0.391),the monthly assessment score of the nursing department(t=3.823),the quality of care increased(x2=5.495),while the nursing adverse events reduced(x2=6.441),and the difference was statistically significant compared with before the training(P＜0.05).Conclusion Daily half-hour case study method can improve the nursing staff comprehensive quality and scientific research ability,the patients' satisfaction,and reduce the nursing adverse events,which is helpful to clinical practice.

Gemcitabine is a new active drug in the treatment of advanced non small cell lung cancer (NSCLC). It is validated that the response rate of the single agent gemcitabine was consistently above 20%, median survival was 34 weeks and it could improve the disease related symptoms with well toleranced toxicities by many clinical studies. This report reviews the clinical studies of gemcitabine in the patients with advanced NSCLC.

Objective:To establish the HPLC fingerprint of Qinghuo Zhimai tablets and determine the contents of geniposide,andrographolide and dehydroandrographolide.Methods:The analysis was performed on a CAPCELL PAK C18 column (250 mm×4.6 mm,5 μm) with acetonitrile and water as the mobile phase with gradient elution at the flow rate of 1.0 ml·min-1.The detection wavelength was 225 nm,and the column temperature was 40℃.Totally 13 batches of Qinghuo Zhimai tablets from different manufacturers were analyzed to establish the fingerprint of Qinghuo Zhimai tablets.Results:There were 14 common peaks calibrated,and the HPLC fingerprint common pattern was established for the HPLC fingerprint of Qinghuo Zhimai tablets.Using the method of reference substance attribution,3 compounds were determined as geniposide,andrographolide and dehydroandrographolide.The similarity of 9 batches in the 13 batches of Qinghuo Zhimai tablets was higher than 0.9.The content determination of geniposide,andrographolide and dehydroandrographolide showed good linearity (r>0.999 8,n=6),the average recovery was 96.90%,97.55% and 97.07% and RSDs were 1.61%,1.45% and 1.47%(n=9),respectively.Conclusion:The method is simple and accurate with good repeatability,which can be used for the identification and evaluation of Qinghuo Zhimai tablets,and provides important scientific basis for the quality control of Qinghuo Zhimai tablets.

Objective To investigate the value of laparoscopy in the diagnosis and treatment of unexplained chronic abdominal pain.Methods From January 2014 to February 2016, clinical data of 56 unexplained chronic abdominal pain undergoing laparoscopic surgery were analyzed retrospectively.Laparoscopic exploration was performed firstly and then the corresponding abnormalities were diagnosed and dealt with accordingly.Results The disease causes were explicit in 48 cases (85.7%), including 25 cases of abdominal adhesions, 8 cases of chronic appendicitis, 2 cases of small intestinal diverticulum, 2 cases of intestinal tuberculosis, 2 cases of lymphoma, 5 cases of endometriosis, 2 cases of tubal adhesions, 1 case of peritoneal metastasis of gastric cancer after surgery, and 1 case of appendiceal mucinous adenocarcinoma.Surgical treatment was performed in 43 cases, 3 of whom were converted to laparotomy.There were no postoperative complications in the 56 cases.The follow-up rate was 92.9% (52/56), and lost of follow-up was in 4 cases.The follow-up duration was from 3 months to 3 years (median, 19.4 months).There was 1 death case (peritoneal carcinomatosis).There were 35 cases (62.5%) of complete relief of abdominal pain (no recurrence of preoperative abdominal pain symptoms) and 12 cases (21.4%) of pain relief (preoperative pain decreased or interval extended).The total effective rate was 83.9% (47/56).Conclusion Laparoscopy is of high value in the diagnosis and treatment of unexplained chronic abdominal pain.

Objective To investigate the clinical characteristics of children with dyspepsia diarrhea and the effect of Clostridium butyricum powder combined with changweikang granules on the treatment of the children with this diseases.MethodsFrom February 2013 to October 2015 in department of pediatrics, Tonglu County the First People's hospital, 76 cases with dyspepsia diarrhea as the research object, were randomly divided into the experimental group and the control group, 38 cases in each group.The two groups were given changweikang granules, at this basis, the observation group were given clostridium butyricum powder.3 days for a course of treatment, the two groups were treated for 2coureses continuously.ResultsBefore treatment, there were no significant differences in clinical symptom scores between the two groups.After treatment, the scores of loss of appetite, abdominal pain, diarrhea, abdominal distension and stool in the experimental group were lower than those in the control group, the difference was statistically significant (P<0.05);Before treatment, the serum motilin, gastrin and somatostatin levels in the two groups were not statistically significant.After treatment, the serum motilin and gastrin levels in the experimental group were lower than those in the control group, and the level of serum somatostatin was higher than that of the control group, the difference was statistically significant;After treatment, the total effective rate in the experimental group was 92.11% higher than that in the control group (73.68%), and the difference was statistically significant (P<0.05).ConclusionPediatric indigestion diarrhea often showed loss of appetite, abdominal pain, diarrhea, abdominal distension and stool.The effect is good which clostridium butyricum powder combined with changweikang granules on the treatment of infantile indigestion diarrhea, it is worthy of clinical application.