Objective To evaluate the influence of extraocular counterpulsation on ocular fundus blood vessels using high-frequency color Doppler ultrasonography. Methods Twenty-one patients with ischemic optic neuropathy were treated with extraocular counterpulsation. During treatment, the Doppler spectra were detected with high-frequency color Doppler ultrasonography. The peak systolic velocity (PSV), end diastolic velocity (EDV) and resistance retinal index (RI) of central retinal artery and posterior ciliary artery were measured instantly before treatment and 1 min,5 min and 15 min after treatment. Results The instant PSV and EDV of central retinal artery and posterior ciliary artery were significantly increased (P＜0.05) after treatment, then decreased gradually. Fifteen min after treatment ,they returned to the level before treatment. Conclusions We can observe the changes of ocular fundus blood vessels noninvasively on high-frequency color Doppler ultrasonography. The method may be regarded as one of the basis for evaluating the effect of extraocular counterpulsation.

Objective To study the therapeutic effects of blood component transfusions on acute leukemia complicated with DIC.Methods The blood component transfusions were mainly used to treat 11 patients with acute aranulocytic leukemia and acute lymphatic leukemia complicated with DIC.Results Three cases were cured,one case were obviously effective,two were improved and five were ineffective,which the total effective rate was 54 5% .Conclusion Early diagnosis and blood component transfusions in timely are effective methods to treat the acute leukemia patients complicated with DIC

Objective To investigate the relationship between the plasminogen activator inhibitor 1(PAI 1) promotor region gene polymorphism and cerebral stroke.Methods The genotype of 4G/5G allele polymorphism in the PAI 1 promotor region were determined by polymerase chain reaction from leukocytes of 60 normal controls and 96 patients with cerebral stroke. Their plasma PAI 1 activity was assayed by ELISA.Results The plasma PAI 1 activity level in CI group was significantly higher than the CH and the control group.The plasma PAI 1 activity level (homozygote 4G/4G genotype patients) in CI and CH group was the highest,5G/5G genotype was the lowest,heterozygous 4G/5G genotype was in the middle;the difference was remarkable between 4G homozygote genotype and the other two groups,there was no remarkable significance between 4G/5G and 5G/5G genotype.There was remarkable difference between 4G/4G homozygote genotype in CI group and the controls(P 0.05 ).There was remarkable difference between the plasma PAI 1 activity in female patients with CI 4G homozygote genotype patients and the male patients of the same genotype (P

[Objective] To evaluate the results of open reduction with bone autografting and calcaneus reconstruction with internal fixation in treatment of intra-articular calcaneus fracture. [Methods]From January 2005 to May 2007, 17 cases/19 feet of intra-articular calcaneus fractures received treatment of reconstruction plate and bone autografting, with 15 male/18 feet, 2 female/ 2 feet. Patients’ age ranged from 19 to 67 years (mean, 37.5 years). Fracture occurred in left side in 9 cases, right in 6 cases, both sides in 2 cases. All patients received treatment of open reduction with bone autografting and calcaneus reconstruction with internal fixation.[Results]All the patients achieved bone union. One stage union was achieved in 15 feet. Poor blood-supply was found in 3 feet and bone union was achieved after systematic treatment. Wound exudation was found in 1 feet, and bone union was achieved after dressing change treatment. All patients were followed up for an average of 19.7 months (10-38 months). The average union time was 2.5 months, without infection found.X-ray and CT were used to measure Bhler angle, width and axial length, all the indexed showed significant difference (P

Objective:To investigate the relationship between the plasminogen activator inhibitor 1(PAI 1) gene polymorphism and cerebrovascular disease and whether it played an important role in the pathogenesis of cerebrovascular disease Methods:The 4G/5G allele polymorphism in the PAI 1 gene promotor region were genotyped by polymerase chain reaction (PCR) from peripheral blood leukocytes from 60 normal controls, 65 patients with acute cerebral infarction and 31 hypertensives complicated with cerebral hemorrhage Their plasma PAI 1 activity were assayed by ELISA Results: The plasma PAI 1 activity level in the CI group was significantly higher than those in the other two groups PAI 1 level was significantly higher in 4G allele homozygous than both those in 4G/5G heterozygous and 5G homozygous Although PAI 1 level was higher in 4G/5G heterozygous than that in 5G homozygous, the difference had not reach statistically significant There were significant differences between acute cerebral infarction PAI 1 gene polymorphism and controls (P0 05), and PAI 1 levels of the 4G/4G genotype among female patients with CI were higher than those of the male patients with CI of the same genotype Conclusion: The results suggest that PAI 1 gene polymorphism may be a susceptible factor to acute cerebral infarction in Chinese, and 4G allele homozygous genotype may be the major risk factor of acute cerebral infarction, especially it may be an independent risk factor of female CI patients

ObjectiveTo evaluate combined pneumatic lithotripsy and vacuum suction during ureterorenoscopy.MethodsCombined pneumatic lithotripsy and vacuum suction during ureterorenoscopy was conducted for 52 cases of urolithiasis with the average age of the patients 36 years old.There were 3 cases of renal stone,9 upper ureteral stone,12 middle ureteral and 28 lower ureteral stone.The average surface area of the stones was 79 mm2.ResultsThe average operation time was 31 min and the fragmentation in situ 100.0%.The stone upward displacement rate was 1.7%.Fragmentation of the stone has been incomplete in 2 cases and ESWL was instituted.49 patients have been followed up for 1～3 months and the stone clearance rate was 96.0%.ConclusionsThe procedure would reduce the high instillation pressure which might cause upward displacement of the stone and liquid reflux to the renal parenchyma.The vacuum pressure set to -0.2～-0.4 bar is the most ideal.

0. 05) , except that anemia and emaciation were more common in the elderly group than that in young group (P

Purpose:To explore the relationship between Epstein-Barr virus(EBV) infection and gastric carcinoma and the role of EBV lytic genes in the tumorigenesis of EBV-gastric carcinomas. Methods:185 gastric carcinoma tissues and 185 corresponding para-carcinoma tissues were tested for EBV genome by polymerase chain reaction (PCR)-southern analysis. EBV-encoded small RNA 1(EBER1) of the PCR positive specimens was detected by in situ hybridization (ISH). Gastric carcinoma with positive EBER1 signals was confirmed as EBV-positive gastric carcinoma. RT-PCR and Southern blotting were used to detect the expression of EBV lytic genes (immediately early genes BZLF1 and BRLF1, early genes BARF1 and BHRF1, late genes BcLF1 and BLLF1) in EBV-positive gastric carcinomas. Results:There were 13 EBV positive samples in gastric carcinomas (7.03%). No EBV positive sample was found from corresponding para-carcinomas. The difference of the EBV positivity was significant between carcinoma and corresponding adjacent carcinoma tissues(? 2= 11.0769,P=0.0009). In our series, age, pathological differentiation, clinical stages, lymph node metastasis and location of cancer were not different between EBV-positive and EBV-negative gastric carcinomas in (P=0.973, 0.141, 0.259, 0.586, 0.062, respectively), while sex was significantly different between EBV-positive and EBV-negative gastric carcinomas(? 2=5.2317,P=0.021). The EBV positivity of male was higher than that of female. Of the 13 EBV-associated samples, 7 exhibited BcLF1 transcript and 2 exhibited BHRF1 transcript. The transcripts of BZLF1 were detected in 6 cases, and those of BARF1 also in 6 cases. No BLLF1 and BRLF mRNA were detected in the 13 EBV-positive samples. Conclusions:EBV infection is associated with the development of gastric carcinoma. Lytic EBV infection occurs in part of the EBV-associated gastric carcinomas, and early genes BARF1 and BHRF1 may play an important role in the tumorigenesis of the EBV-positive gastric carcinoma.

Objective To explore the role of Epstein-Barr virus (EBV) infection in the etiology of drug eruption. Methods PCR-Southern blot was used to detect EBV-specific DNA fragment BamH I -W in peripheral blood mononuclear cells of 32 patients with drug eruption and 30 age- and sex-matched normal controls. The mRNA expression of EBV lyric gene BZLF1 in EBV DNA-positive samples was measured by RT-PCR and Southern blot. ELISA was performed to detect EBV virus capsule antigen (VCA)-specific IgM. Results The positivity rate of EBV DNA was significantly higher in patients with drug eruption than in normal controls (78.13% (25/32) vs 10.00% (3/30), P < 0.01), while no significant difference was noted between patients with severe and mild drug eruption (P > 0.05). The expression of BZLF1 mRNA was detected in 3 out of 25 EBV DNA-positive patients; of the 3 patients, 1 suffered from mild drug eruption, and 2 from severe drug eruption. EBV VCA-specific IgM was observed in 6 of 32 patients with drug eruption, but not in any normal controls. No significant difference in the positivity rate of EBV VCA-specific lgM existed between patients with severe and mild drug eruption (P > 0.05). Conclusions There is an active infection of EBV in patients with drug eruption. EBV infection is probably an environmental factor affecting the development of drug eruption.

Animals ; Blood Pressure ; Heat-Shock Proteins ; metabolism ; Heat-Shock Response ; Hypotension ; metabolism ; Male ; Myocardium ; metabolism ; Nitric Oxide ; metabolism ; Rats ; Rats, Sprague-Dawley