Objective To explore the effect of antidepressant adjuvant treatment for chronic prostatitis (CP),and to find an effective treatment for the clinical therapy of CP.Methods 88 patients with CP were randomly divided into two groups,the control group 41 patients were treated with the antibacterials moxifloxacin,the treatment group 47 patients were treated with the moxiiloxacin treatment and paroxetine adjuvant therapy.The clinical symptoms,life quality (QOL) and Zung depression scale score of the two groups were evaluated and compared.Results After four weeks of treatment,voiding difficulty endless,urinary urgency,perineal discomfort symptoms of the treatment group were improved significantly compared with the control group (x2 =23.563,18.348,15.874,all P ＜ 0.05).After four weeks of treatment,QOL,Zung depression scale scores the treatment group were more significant decline than before treatment and the control group after treatment (t =0.554,0.615,0.382,0.336,all P ＜ 0.05).Conclusion Antidepressant adjuvant therapy improves the psychiatric symptoms of CP patients,which can effectively improve the clinical efficacy of antibiotic therapy of CP.

Objective To detect the expression of telomerase catalytic subunit (TERT),telomerase in human ovarian luteinized granulosa cells and to investigate the relationship between telomerase expression and the ovarian fecundity Methods Ovarian luteinized granulosa cells were recovered from 22 women with regular menses who underwent in vitro fertilization/ intracytoplasmic sperm injection programme.We carried out in situ hybridization histochemistry on luteinized granulosa cells to detect TERT mRNA expression,and telomeric repeat amplification protocol to detect telomerase activity Results TERT mRNA were present in luteinized granulosa cells.The expression of TERT mRNA and telomerase activity in ovarian luteinized granulosa cells decreased with increase of age,basal serum follicle stimulating hormone levels Conclusions These results suggest that telomerase may play an active role in ovarian function.A reduced telomerase activity of granulosa cells may be one of the important mechanisms involved in decreased ovarian function in women.

Objective: To investigate the effects of Zhu Yun Pill(ZYP) in the treatment of infertility due to luteal phase defect(LPD).Methods: 163 infertility patients due to LPD were randomly divided into three groups in terms of ZYP group,progesterone group and combination group(ZYP and progesterone).Then the therapeutic efficacy was judged by the pregnancy rate(PR),endometrial biopsy,the level of serum hormone and basal body temperature before and after the treatment.Result: 40 women were pregnant in ZYP group(cure rate,33.33%),8 in the progesterone group(cure rate,26.67%) and 9 in the combination group(cure rate, 69.23%).The symptomatic amelioration and total effective rate of ZYP group were obviously higher than single exogenous progesterone group(P

Objective To evaluate the effect on reliability with different sample amount when human corneal endothelial morpho- logic parameters estimated using center by center method analysis.Design Clinical study.Participants Thirty central corneal endothe- lium photo-micrographs for the same eye. Methods Noncontact specular photomicrographs were obtained.Each photo-micrograph was analyzed with center by center method and was analyzed for 9 times by pointing different amount cells,from 10 to 90.Compare the di- versity of corneal endothelial morphometric parameters between different groups. Main Outcome Measures Corneal emtothelium den- sity,coefficient of variation.Results The coefficient of variation decreased and then kept to a same level when sample cell amount were added from 10 to 90.With the sample cell amounts varied from 10,20,30,40,50 to 90,the coefficient of variation was changed from 9.5,6.3,4.6,4.4.,4.3 to 3.8.Corneal endothelium density had significant difference between the first two groups of 10 and 20 cells with the other groups(P

Objective To explore 24-hour ambulatory blood pressure of patients with cervical spinal cord injury. Methods 42 patients with cervical spinal cord injury and 45 patients with thoracic-lumbar spinal cord injury were included. Their 24-hour ambulatory blood pressures were monitored. Their day systolic blood pressure (dSBP) and day diastolic blood pressure (dDBP) when seating and standing, night SBP and DBP, and SBP descending rate at night were compared. Results There were significant differences in dSBP and dDBP when standing, and SBP descending rate at night between 2 groups (P<0.01). There was significant difference in dSBP between seating and standing position in patients with cervical spinal cord injury (P<0.01). Conclusion There is orthostatic hypotension in cervical spinal cord injury patients and their SBP descending rate at night is upside down.

Objective To investigate a mechanism protected radiation-induce injure for radiated guinea pigs'nasal mucosa treated with intranasal corticosteroids (fluticasone nasal cavity spray). Methods 50 health guinea pigs were divided into 2 groups randomly: the irradiated group (control group) with 25 guinea pigs and the administration group after irradiation (test group)with 25 guinea pigs. The nasal part of all guinea pigs were performed irradiation by the 6 MV X-ray with single 5 Gy, one time each week for three weeks. The guinea pigs of test group received intranasai eorticosteroids with one time every day and one spray each side nasal cavity on the second day after three weeks irradiation. Five guinea pigs in each group were saeritieed randomly at 1 week, 1 month, 2 months, 3 months and 4 months after irradiation, and the histopathologie changes were observed under optical microscope and electron microscope. At the same time, blood were drawn from the heart and the concentration of IL-1, IL-6 and TNF-α in serum were measured by ELISA. Results The early nasal mucosa inllammatory reaction of the test group was less than the control group. The coverage rate of cilia of the test group was much than that of the control group (72.9% vs 50.2%) at four months after irradiation. The atrophy of submucosal glandular organ was lessened and they displayed some extent secretory function. The concentration of IL-1 in serum was increased very much in the test group compared with the control group after irradiation and kept higher level in the first two months. After two months, it began to decrease;on four months, it still kept equivalency level with the control group. The concentration of IL-6 and TNF-α in serum were reduced all the while. Conclusions The intranasal eortieosteroids with fluticasene nasal cavity spray can reduce radiation-induced injury of guinea pigs' nasal mucosa. The concentration change of IL-I, IL-6 and TNF-α in serum may be one of mechanism protected radiation-induce injure of guinea pigs' nasal mueosa.

Terapeutic options used to repair tendon and ligament injury consist of autografis,allografts and synthetic prostheses.Tendon and ligament of tissue engineering have their unique advantages as alternative therapy.Choosing the proper scaffold materials is important for successfully constructing tissue engineering tendon and ligament,so in this review we focus on natural and artificial scaffold materials used in tissue engineering of tendon and ligament in recent years.

In this article we present the types of wireless sensor network (WSN) and their application in the medical field,including patient monitoring,disability assistance,emergency rescue and biological monitoring.Then we propose the challenges that WSN has to face in medical field concerning the development issue of safety,energy consumption and reliability.Finally,foreground of the WSN technology is prospected.

Objective To explore the diagnosis and treatment of unsuspected gallbladder cancer (UGC)related with laparoscopic cholecystectomy(LC).Methods From January 1995 to Dece m ber 2008,4520 cases of LC were performed,UGC waS discovered in 15 cases.Clinical data of the 15 cases were analyzed retrospectively.Results Among the 15 cases of UGC,7 were discovered intraoperatively,and 8 were diagnosed postoperatively.Postoperative pathological examination showed Nevin stage I in 4 cases,stage Ⅱin 7 cases,stage Ⅲ in 3 cases,stage Ⅳ in 1 case.LC was carried out in the patients of Nevin stage I.Radical resection of gallbladder cancer was performed on the patients at stage Ⅱ and stage Ⅲ.One patient at stage Ⅳ received extended radical resection.The patients were followed up from 6 months to 5 years,during the period 3 cases were died and 12 cases were still alive,the 5-year survival rate wag 80.0%.Conclusions Most of LC-related UGC cases are early cancer.Frozen section examination should be performed for high-risk groups and suspicious persons,which contributes to the discovery of UGC.LC can achieve the radical purpose of Nevin stage I patients,Nevin stage Ⅱ-Ⅳ requires open radical operation.

Objective To investigate the relationship between serum leptin levels and insulin resistance(IR)in obesity and hypertensive patients and their role on the occurrence of hypertension or/and obesity.Methods Serum glucose,insulin,total cholesterol(TC),triglycerides(TG),HDL－ C,LDL－ C,uricacid(Ur),insulin sensitivity index(ISI),urinary albumin excretion(UAE)and serum leptin concentrations were determined in 66 men with essential hypertension and 67 male normotensives.The correlations between leptin and IR,and they and body mass index (BMI),blood pressure and other parameters were analyzed.Results Serum leptin levels were signifcantly higher in hypertensive and obesity subjects than in normotensive and nonobesity ones.ISI of nonobesity was higher than obesity in normotensive group,but both obesity and nonobesity ISI was higher in normotensive group than in hypertensive one .No significant change in obesity and nonobesity was found for the decreased levels and ISI(r=－ 0.51,P<0.01 and r="－" 0.38,P< 0.05,respectively ) and the most singificant factors that affected serum leptin levels were BMI,waist hip ratio and ISI and the factors that affected ISI in order of importance were BMI,SBP,DBP,TG,Ur and leptin in obesity subjects of both hypertensive and ISI in above mentioned patients.The multiple regression analysis indicated that BMI was closely correlated with leptin,ISI and TG in obesity patients of both hypertensive and normotensive groups. Conclusion The increase in serum leptin levels in obesity is highly correlated with ISI and is correlated with lipid metabolism.leptin resisitance may has a indirect action in occrrence of hypertension and the correlation with IR remain to be sutdied.