Objective To explore the effect of antidepressant adjuvant treatment for chronic prostatitis (CP),and to find an effective treatment for the clinical therapy of CP.Methods 88 patients with CP were randomly divided into two groups,the control group 41 patients were treated with the antibacterials moxifloxacin,the treatment group 47 patients were treated with the moxiiloxacin treatment and paroxetine adjuvant therapy.The clinical symptoms,life quality (QOL) and Zung depression scale score of the two groups were evaluated and compared.Results After four weeks of treatment,voiding difficulty endless,urinary urgency,perineal discomfort symptoms of the treatment group were improved significantly compared with the control group (x2 =23.563,18.348,15.874,all P ＜ 0.05).After four weeks of treatment,QOL,Zung depression scale scores the treatment group were more significant decline than before treatment and the control group after treatment (t =0.554,0.615,0.382,0.336,all P ＜ 0.05).Conclusion Antidepressant adjuvant therapy improves the psychiatric symptoms of CP patients,which can effectively improve the clinical efficacy of antibiotic therapy of CP.

Objective To explore the regulating mechanism of the astrocytes on the expression of ?-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors(AMPARs) subunit in epileptogenesis. Methods The astrocytic conditional medium(ACM) was collected after being stimulated by glutamate,and then ACM was added to the cultured hippocampal neurons.The expression changes of neuronal GluR2 and protein interacting with C-kinase-1(PICK1) mRNA were detected by RT-PCR. Results In the cultured hippocampal neurons,the GluR2 mRNA expression was significantly decreased at the 2nd,8(th),and 12(th) hours after the administration of ACM compared with that in the control group(P

Objective To explore the effect of insulin-like growth factor-1(IGF-1) on the growth of muscle-derived stem cells(MDSCs). Methods MDSCs were isolated from the hindlimb muscle of new born mice through serial preplates.DMEM with 2% fetal bovine serum was used to induce MDSCs to differentiate into skeletal muscle lineage.The expression of stem cell marker Sca-1 or skeletal muscle cell marker ?-sarcomeric actin was examined by immunocytochemistry SP method.MTT colorimetric microassay was conducted to determine the effect of IGF-1 on MDSCs proliferation.The relationship between functioning and culture time and the relationship between functioning and IGF-1 concentration were studied. Results MDSCs were successfully isolated from the hindlimb of neonatal mice.Over 90% of the MDSCs showed Sca-1 positive immunoreactivity.MDSCs could give rise to ?-sarcomeric actin positive myotubes in differentiation cultures.The proliferative effect of IGF-1 on MDSCs increased gradu ally with prolonged culture time.The elevation of IGF-1 concentration also increased the proliferative effect until it came to a saturation point.Conclusion IGF-1 can promote the proliferation of in vitro cultured MDSCs.

Objective To explore the clinical role of transbranchial needle aspiration(TBNA) in the lymph node staging of lung cancer.Methods To forty-six cases of lung cancer with suspected lymph node metastasis by chest CT scan,the TBNA was performed before operation.The cytological results were compared with post-operative pathology.Results In total 94 groups mediastinal nodes,fifty-nine groups were positive by TBNA,sixty-seven groups were diagnosed lymph node metastasis by pathology,the positive accuracy was 88% with the total coincidence rate of 82.6% by cytolagical typing.The overall accurate rate of c-N by TBNA was 86.9%(40/46) compared with p-N.Conclusion TBNA for lymph node staging and pathologic typing of lung cancer is a safe,simple and economic method with high accuracy and high clinical application value.

Objective To establish a method for the determination of aluminium in drinking water by flow injection analysis instrument.Methods In a hexamine buffer solution(pH=6.0),phenanthroline solution and ascorbic acid as the anti-interference agent,aluminium could react with chromazurol S solution and form a compound,of which maximum absorption was at 550 nm.Results The linear range for this method was 5-200 ?g/L.The correlation coefficient was 0.999 3,the detection limit of this method was 2.0 ?g/L,the recovery rates were in the range of 92.2%-108% and RSD were in the range of 2.5%-8.2%.Conclusion The flow injection method is simple,rapid,accurate and is applicable to the determination of aluminium in drinking water.

To study the effect of using a steel stainless intrauterine device(IUD) on the incidence of anemia and iron deficiency and thus to find a suitable way to prevent or cure it, the serum ferritin level of 176 women bearing such an IUD was determined (FER-IRON kit, Ramco laboratories, USA). As compared with the control group which was composed of 163 women having been performed with tubal sterilization, no distinct difference in hemoglobin levels and red cell counts was observed, but the serum ferritin level in IUD-bearing group decreased markedly (26.6?1.4 vs. 36.3?2.2 ng/ml, pears). When treated with ferrous sulfate (0.3 g, t.i.d.) for 7 days, the ferritin level of the group of these IUD users raised up to that of the control group rapidly. It was suggested that the supplement of iron in time for the IUD users suffering from menorrhagia was necessary and efficient.

Five strains out of the 8 of epidermic hemorrhagic fever(EHF)virus isolated from the serum of the acute phase of EHF patients in Nanchong, Sichuan were found to have cytopathogenic effect(CPE)through 4th to 10th passages in Vero-E6 cell culture. The biological, physico-chemical and antigenic characteristics of HFN-04 and HFN-19, 2 out of the 5 CPE strains, were identified. The results were as follows;( 1 ) The CPE development and the dynamic proliferation of the virus areconsistent. ( 2 ) The main biological and physico-chemical characteristics of the 2 CPEstrains arc the same as those of EHF virus.( 3 ) When paired samples of EHF patients' serum were tested with the slide antigens of E6 cells infected with these CPE strains, all the convalescent serum demonstrated an increase by 4 fold or more of the IFA. titer.( 4 ) The CPE of the virus can be neutralized by EHF patient's serum or EHF and KHF antisera, but not by normal rabbit serum or the multivalent serum of Reovirus type 1-3.( 5 ) The E6 cells infected by CPE strains of virus can almost fully absorbthe specific antibodies in EHF patients or in EHF and KHF antisera.( 6 ) The suspension of the mouse brain infected by CPE strains can formpositive agglutination reaction with the sentitized blood cells by EHFvirus strain A9 McAb 25-1.On the basis of our observation, it can be concluded that at least certain strains of EHF virus can produce CPE in Vero-E6 cell culture.

Dietary survey, physical examination and blood tests were carried out on kindergarten children from two to seven-year old in Wuhan, excluding some influencing factors such as malnutrition and so on. Three groups, 50 children each, were differentiated according to serum vitamin A level .avita-minotic (011 mmol/L). Immunological parameters were determinated before and after vitamin A supplementation. The results showed.'there were significant differences of S-IgA,C3 and lymphocyte transformation active response to PHA and lysozyme between vitamin A deficiency and normal group, but not on IgG, IgA, IgM and PHA skin test. After vitamin A supplementation these immunological functions could be improved. So we concluded vitamin A in subclinic deficiency could influence immune function too, but it could be recovered through vitamin A supplementation.

The chemical nature of spinal ganglionic neurons, the peripheral processes of which project divergently to the somatic and visceral areas, has been identified by means of tri-labeling method of combining fluorescein tracing and immunocytochemistry. Ten rats were used. First, 2?l of 2% fast blue (FB) were injected into the left coeliac ganglion. Two days later, 2% nuclear yellow (NY) was injected into left 9-11th intercostal nerves(l?l for each). On the 4th day, animal was perfused with 10% formalin in 0.1mol/L phosphate buffer.The left Th9-11 spinal ganglia were removed and cut into sections by cryostat. The sections were observed under fluorescence microscope and photographed. The results showed that there were three kinds of neurons in the spinal ganglia: (1) single FB labeled cells with blue fluorescent cytoplasm accounted for 38.8% of total labeled cells; (2) single NY labeled cells with yellow fluorescent nuclei accounted for 52,7%; (3) FB and NY double labeled cells accounted for 8.5% and mostly were small or medium in size. Then, the double labeled cells-containing sections were further processed by substance P-demonstrating PAP immunocytochemical staining. The immunostain and fluorescent photographs in the same section were compared and identified each other. We have found that the labeling ratio of SP/NY was 1.4%; SP/FB was 7% and SP/NY+FB was 28.8%. Present study has not only identified the convergence of somato-vesceral sensation in spinal ganglia but also detected the chemical nature of these neurons(substance P) for the first time. In addition, this result has provided a morphological basis for the mechanisma of referred pain and somato-visceral reflection.