OBJECTIVE:To investigate the reform mode of experiment teaching in pharmaceutical analysis in the new situation of vocational education and provide reference for improving the teaching quality of secondary vocational education. METHODS:The present situation of experiment teaching in pharmaceutical analysis was assessed deeply and in detail,and the problems exist-ing in teaching were analyzed;the reform plan and measures according to the disadvantages of the current teaching were raised. RE-SULTS:The experiment teaching in pharmaceutical analysis existed some problems,involving lagging textbook compilation,single teaching mode,“heavy theory,light practice”for time arrangement,obsolete laboratory apparatus and lack of advanced equipments. So a series of measures were adopted,including writing supplementary teaching materials and experimental guidance;reforming the contents of the experiment teaching;reforming the model of teaching and improving the students’participation;reforming the time arrangements;increasing the investment in laboratory equipment and attaching great importance to extramural cooperation;estab-lishing the virtual laboratory;and establishing a flexible,comprehensive and effective experimental evaluation mechanism.CON-CLUSIONS:Reform experimental teaching mode has fully mobilized the enthusiasm and initiative of students and improved the abil-ity of operating skills,and cultivated the strict scientific research style of students. The reform mode of experimental teaching in pharmaceutical analysis is in accordance with the students’learning needs of pharmaceutical majors,and lay a good foundation for the students better and faster adaption to the job.

Epidemiologic studies suggest that intake of excess all-trans-retinoic acid (RA) during embryogenesis induces various developmental defects and the central nervous system (CNS) represents a major site of the teratogenic action of RA. It is therefore important to understand which parameters are affected early by excessive RA in order to devise and improve protective nutritional strategies. The modulations of beta-catenin and caspase-3 levels were investigated in the KM mouse embryo following maternal treatment with a single oral dose of 30mg/kg body weight of RA during the neurula period. In addition, retinoic receptors (RARs) are key transcription factors regulating gene expression in response to RA-activated signals. So the experiment was designed to evaluate whether the alterations in protein expression of RAR alpha and beta during the time of neural tube closure were induced by excessive RA. Maternal intake of excess RA induced early downregulation of RAR alpha and beta, beta-catenin and caspase-3 expression, which was followed by an increase in their expressive levels in the neural tube tissue of mouse embryos. This finding suggests that the alterations in the expression profile of RAR alpha and beta, beta-catenin and caspase-3 may be implicated in the teratogenesis induced by excess RA in KM mouse embryo.

BACKGROUND: Yishen jiangu granule is one of sheer traditional Chinese drugs, which has good effect of tonifying kidney, strengthening bone, reinforcing the spleen, nourishing qi and improving the body condition, mainly used to prevent the lassitude, rickets, osteomalacia and calcium-deficiency induced by kidney-deficiency.OBJECTIVE: To observe the preventive effect of yishen jiangu granule on the reproductive organs, physical strength as well as the ability of stress, and compare with the effect of longmu zhuanggu preparation which was characterized by invigorating spleen and replenishing qi, and tonifying kidney and replenishing essence.DESIGN: Completely randomized controlled design.SETTING: Institute of Traditional Chinese Medicine, Chengde Medical CollegeMATERIALS: A total of 180 Kunming mice, weighing 25-30 g, of half gender, of clean grade, were selected in this study. Longmu zhuanggu granule, which was composed of root of tangshen, tuckahoe, rhizome of lagehead atractylodes, huai yam, astragalus root, schisandra fruit, keel,oyster shell, tortoise plastron, dwarf lilyturf, Chinese date, licorice root,vitamin D, etc., was provided by Wuhan Jianmin Pharmaceutical Factory (batch number: 960618). Yishen jiangu granule, which was composed of 6 kinds of traditional Chinese medicine such as dark plum, Chinese wolfberry, oyster shell, etc., was manufactured by Institute of Traditional Chinese Medicine of Chengde Medical College (batch number: 950510).Hydrocortisone injection was produced by Bohai Pharmaceutical Factory of Tangshan city (batch number: 940303).METHODS: The experiment was completed in Institute of Traditional Chinese Medicine, Chengde Medical College from January to October 1997. ① A total of 180 mice were randomly divided into 6 groups with 30 in each group: normal group (5.0 mL/kg saline, muscle injection); model group (25 mg/kg hydrocortisone for the mice model with kidney-yang deficiency, muscle injection); longmu zhuanggu granule group (5.0 g/kg longmu zhuanggu granule, and 25 mg/kg hydrocortisone); three groups of yishen jiangu granule at different dosages (perfused respectively with yishenjiangu granule at the dosages of 1.25 g/kg, 2.5 g/kg and 5.0 g/kg;meanwhile, 25 mg/kg hydrocortisone, muscle injection). The mice were administered successively for 14 days. ② In 12 hours after giving the last medicine, each group was divided into 3 parts to go on with the following 3 sub-experiments: Firstly, the spermary and uterus of mice were taken out to determine their weight, then calculate the indexes (mass of uterus/body mass of mice); secondly, the mice were put into the water [water depth:30 cm, water temperature: (20±0.5) ℃], burden of tail of mice was weighed 10% of the body mass, then timed immediately, when the mice could not immerge from the water after their heads sank into the water for 10 seconds.It showed that they were exhausted (the time was regarded as the swimming time of mice); thirdly, 10 g sodalime was added into each bottle with wide neck whose volume was 150 mL, then one mouse was put into each of them,covered them and timed immediately. The breath of the mice was observed until they stopped breathing, then timing was over. This was the bearing time of mice on anoxia under normal pressure. ③ Difference of statistical significance between groups was determined by t-test.MAIN OUTCOME MEASURES: The mass indexes of reproductive organs, swimming time and the bearing time on anoxia under normal pressure of the mice in all groups.RESULTS: A total of 180 mice were all involved in result analysis. ① The mass indexes of spermary in mice in normal group, longmu zhuanggu granule group and yishen jiangu groups at high and middle dosages were significantly higher than those in model group (P ＜ 0.05-0.01); the mass indexes of uterus in mice in normal group, longmu zhuanggu granule group and yishenjiangu groups at high dosage were significantly higher than those in model group (P ＜ 0.01). ② The swimming time of the mice in model group was significantly shorter than that in the other 5 groups (P ＜ 0.01). ③The bearing time on anoxia under normal pressure of the mice in model group were significantly shorter than that in the other 5 groups (P ＜ 0.01).CONCLUSION: Yishen jiangu granule has the remarkable effect on kidney-tonifying. It can remarkably relieve the reproductive organ atrophy of the mice with kidney-yang deficiency, has remarkable effect on antifatigue, and also can increase the ability of stress.

Objective To investigate the clinical effect of Gonadotropin-releasing hormone agonist (GnRHa) combined with addback therapy after laparoscopic surgery on 153 cases with severe endometriosis patients. Methods 153 cases with severe endometriosis patients who underwent laparoscopic surgery from 2011 to 2013 were retrospectively analyzed in the Second Affiliated Hospital of Zhengzhou University. According to GnRHa or addback therapy three groups were divided:GnRHa group,GnRHa and addback therapy group, control group. The curative effects of different treatment group, adverse reactions, recurrence rates, and fertility were analyzed. The follow-up time was 6-24 months with an average time of 17.15 months. Results 51 patients suffered from obvious menopausal symptoms and received addback therapy (Progynova,0.5~1 mg/d). The VAS score and Kupperman score in GnRHa group,GnRHa and addback therapy group were significantly superior to the control group (P <0.05). And no statistical significance was found in the interval to relapse, recurrence and postoperative pregnancy rate (P>0.05). The VAS scores in the GnRHa group and addback therapy group were 2.14±2.02 and 1.73±2.17 , respectively (P > 0.05). The main side effect of GnRHa group is hot flashes, diaphores, insomnia, sexual dysfunction, etc. The incidences were higher in the GnRHa group than that in the addback therapy group. But the addback therapy can significantly relieve the symptoms of low estrogen introduced by the application of GnRHa (P<0.05). Conclusions Laparoscopic combined with GnRHa treatment can significantly improve clinical curative effect of endometriosis. Addback therapy could abate the side reaction of GnRHa and do not affect the clinical curative effect. The clinical curative effect in the GnRHa and addbacktherapy group were superior to the GnRHa group.

Objective In order to study the effect on chondrocyte proliferation and the mechanism of treating osteoarthritis(OA) by XBL,the experimant is carried out.Method The first－ subcuiture chondrocytes are implanted into two 96－ foramen culture plates and sixteen culture flasks.After 24 hours in cubation 8 groups are divided into as follows:No 1 group(control)5％ serum DMEM No,2～ 7 groups(XBL)5％ serum,0.5％ 、 1％ 、 2％ 、 4％ 、 8％ 、 16％ XBL－ DMEM are added respectively.NO 8 group no cell incubation.After culture of 24 and 48 hours ,two plates are detected for viablilty and proliferation respectively by MTT method(Light absorption value A),cell total protein is assessed by comassie Brilliatnt G－ 250 colormetric assay.Result 2％ ～ 8％ XBL can promote the proliferation and protein synthesis of chondrocyte.Conclusion XBL can protec degradation from Matrix metallo protzinase and decrease the impairment of chondrocyte.

OBJECTIVE:To observe the clinical efficacy and safety of Kangfuxin solution combined with rhEGF solution for external use in the treatment of vitamin B12 deficiency atrophic glossitis. METHODS:Totally 94 patients with vitamin B12 deficien-cy atrophic glossitis in Xinjiang Production and Construction Corps Hospital during Jul. 2012-Jun. 2015 were divided into observa-tion group and control group according to random number table,with 47 cases in each group. Both groups received etiological treat-ment of vitamin B12 supplement. Control group was additionally given rhEGF solution for external use on tongue surface,qid. Based on control group,observation group was additionally given Kangfuxin solution and applied medical gauze saturated with liq-uid medicine to the affected area,more than 30 min each time,tid. Treatment course of 2 groups lasted for 4 week,a referral of the week. Clinical efficacies were compared between 2 groups as well as average pain indexes and lesion areas of tongue before and after treatment,and the occurrence of ADR. RESULTS:Cure rate(76.6% vs. 57.4%)and total response rate(97.9% vs. 83.0%) of observation group were significantly higher than those of control group,with statistical significance (P<0.05). Before treat-ment,there was no statistical significance in average pain indexes and lesion areas of tongue between 2 groups (P>0.05). 1-4 weeks after treatment,average pain indexes and lesion areas of tongue in 2 groups were significantly decreased,with statistical sig-nificance compared to before treatment (P<0.05). The average pain indexes of observation group were significantly lower than those of control group (3,4 weeks after treatment),and lesion areas of tongue was significantly smaller than control group (2-4 weeks after treatment),with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:For vita-min B12 deficiency atrophic glossitis,Kangfuxin solution combined with rhEGF solution for external use can significantly improve tongue lesion and have good analgesic effect and safety.

Objective To evaluate the occurrence of multidrug-resistant organism (MDRO)infection in a general hospital,and take effective comprehensive intervention measures,so as to reduce MDRO infection.Methods Targeted monitoring on MDROs was performed from October 2012 to December 2013,comprehensive intervention measures were conducted,the occurrence of MDRO infection before and after intervention was compared.Results A total of 62 384 pa-tients were investigated,17.91%(n=11 176)were sent specimens for pathogen culture,606 times of culture for patients’ specimens were detected MDROs,292(0.47%)cases were MDRO healthcare-associated infection(HAI),314 were com-munity-acquired infection or colonization.MDRO infection case rate decreased from 0.65%(75/11 603)before intervention to 0.26%(36/13 875)after intervention (χ2 =21.08,P <0.05).Conclusion Comprehensive intervention measures can effectively reduce the occurrence of MDRO HAI.

Purpose:To observe the efficacy of capecitabi ne combined with the transcatheter arterial chemoembolization (TACE) in late-stag e primary hepatic carcinoma.Methods:62 patients of late-stage hepatic carcinoma (TNM: Ⅱ- Ⅳ) were divided into two groups at random:①Simple TACE group: 30 cases;②capec itabine+TACE group:32 cases were given additional oral capecitabine for 14 days the next day after TACE therapy. All the patients were followed for two years.Th e median survival time, the average survival time of dead patients, the 1-year survival rate and the second-stage surgical resection rate of the two groups we re compared. Results:The median survival time of capecitabine+TACE group was 14.5 months, the 1-year survival rate 75%, the second-stage surgical resectio n rate 25%; the TACE group was 6 months,39.3%,6.7% ,respectively. The difference was significant (P

OBJECTIVE:To investigate the drug use in the inpatients of our hospital and evaluate its rationality.METHODS:By using PASS-nested "Army Health No.1" hospital information system,the drug utilization in these patients was analyzed.RESULTS:Among 32 834 medication orders for 586 inpatients,732(1.50%)were warning ones.CONCLUSION:The drug utilization in the inpatients of our hospital was rational on the whole.PASS satisfies clinical doctors' needs,and the physicians feel satisfied with the system.Nevertheless,there are some problems remain to be tackled.

Objective To make clear the knowledge level of emergency treatment during the disasters among emergency nurses,and then make out the countermeasures.Methods Using the self-designed questionary to investigate the knowledge level of emergency treatment during the disasters among emergency nurses.The contents of survey included: general condition,related experiences about emergency treatment,the anxiety and the pattern of how to obtain the related knowledge.Results The level of knowledge of emergency treatment was different in the different nurses according to their different working time.Most of the emergency nurses had not accepted the normal training about the emergency treatment of disasters.Conclusion The nursing administers should put out certain measures to strengthen the training about emergency treatment,and then improve the nurses ability to deal with disaster.