As master genes during embryogenesis, Homeobox(Hox) genes play important roles not only in embryonic development, cellular growth and differentiation and the expression of the specific genes of some tissue, but also in controlling hematopoietic tissue development and differentiation in a germ line -specific and stage-specific. At present studies showed there is a close relationship between Hox genes and tumorgenesis. This article reviews the advances in the research on correlation between homeobox genes and tumorgenesis.

Three components have been isolated from Zebrina pendula Schnizl and identified as ?-sitosterol, 3?, 5?, 6?-trihydroxy stigmast and succinic acid. As a result the anti-tumor rate were 43% (160mg/kg)for succinicacid, 91% (100mg/kg) for ?-sitosterol and 98%(100mg/kg) for 3?, 5?, 6?-trihydroxystigmast, respectively, after anti-cancer test of ascites type-180 sarcoma of mice. It was found that the last hadn't toxicity when its dose was increased.

Objective To explore the role of active macrophages on the vascular endothelial participation in angiogenesis. Methods Vasculuar endothelial cell line ECV-304 was inoculated and cultured to 60% confluence.ECV-304 cells were then cultured lonely, with ConA(24?g/ml), with macrophage cell line U937(1?10 5/ml) or with U937 and ConA together to make four groups.The change of the ECV-304 cell cycle was determined by flow cytometry and that of the DNA synthesis was detected by 3H-TdR incorporation test. The change of the expression of endothelial VEGF receptor KDR mRNA was detected by RT-PCR while that of the integrin receptor ? ? ? 3 expression by immune fluorescent antibody technique on flow cytometry. Results Endothelial cells in S phase increased obviously with increased DNA synthesis in ECV-304 cells stimulated by active macrophages. In addition, the expression levels of the VEGF receptor KDR mRNA and integrin receptor ? ? ? 3 up-regulated significantly(P

Objective To investigate the mechanism of regulation of endothelium participating angiogenesis by macrophage. Methods Human umbilical vein cell line (ECV-304) was cultured. When 60% of the ECV-304 cells became confluent, human macrophage cell line (U937) after being stimulated by concanavalin A (Con A) was co-cultured with them. The cells were then divided into four groups, i.e. control group (pure ECV-304 culture), co-culture group of Con A with ECV-304, co-culture group of U937 with ECV-304, and co-culture group of U937 with Con A and ECV-304. After 48 hours of co-culture, the morphology of ECV-304 cells was observed under invert microscope, and the changes in cell cycle of ECV-304 were determined by flow cytometry. In addition, the changes in the expression levels of homeobox (HOX) B2 mRNA and VEGF receptor KDR of endothelia cells were assessed by RT-PCR. Results In ECV-304 co-cultured with Con A and U937 group, inter-endothelial gaps were found to be widened, cell shape became abnormal with obvious processes simulating neurons, and the endothelial S phase was prolonged significantly (P

OBJECTIVE: To provide reference for effective and safe application of anticancer drugs in the clinic. METHODS: Combined with the practice and experience in PIVAS for 5 years, the evaluation approach of anticancer drugs were analyzed and summarized comprehensively. RESULTS & CONCLUSIONS: The anticancer drugs prescription was evaluated in respect of reasonable dosage, rational solvent, appropriate drug usage, special usage and the reasonability of drug combination, which guarantee the quality of anticancer drug and rational use of drug in the clinic.

Objective To evaluate the clinical practicability of cefoxitin disk diffusion method in detecting methicillin-resistant Staphylococci.Methods 163 strains of clinically isolated Staphylococci were tested.The cefoxitin,oxacillin disk diffusion test and PCR amplification of mecA gene were performed simultaneously.The method of PCR was used as the golden standard.Results 163 strains of clinically isolated Staphylococci were tested by PCR.The positive ratio of mecA gene was 81.0%,The ocurrence rate of methicillin-resistant S.aureus(MRSA) and (methicillin-resistant coagulase-negative Staphylococci(MRCNS) was 74.1% and 83.8%,respectively.The cefoxitin,oxacillin disk diffusion and PCR were high consistency in detecting mecA gene.Both sensitivity and specificity were 100%,But cefoxitin disk diffusion was better for methicillin-resistant coagulase-negative Staphylococci.The sensitivity was 100% and 96.0%,the specificity was 94.3% and 88.0%,respectively.Conclusion Cefoxitin disk diffusion has high sensitivity in detecting methicillin-resistant Staphylococci,but for methicillin-resistant coagulase-negative Staphylococci which mecA gene is negative,5% strain may be reported as MRCNS by mistake.Since cefoxitin disk diffusion method is simple,and has higer sensitivity and specificity than oxacillin disk diffusion method in detecting MRS,the result has good correlation with PCR in detecting mecA gene.It may be applied in clinical microbiology laboratory.

Objective To characterize the effects of Sanchi on the alcoholic hepatopathy rats.Methods 70 SD male rats were randomly divided into five groups:normal group(n=10),control group(n=15),high-dose Sanchi group(n=15),low-dose Sanchi group(n=15),and Tiopronin group(n=15).In order to induce alcoholic hepatopathy,animals in the control,high-dose and low dose Sanchi,and Tiopronin groups were orally dosed with HongXing ErGuoTou(56% ethanol;5g/kg),corn oil(2ml/kg),and pyrazole(27.2 mg/kg)every morning for 14 weeks.Rats in high-dose Sanchi,low dose Sanchi,and Tiopronin groups,were treated every afternoon during the 14-week induction of hepatopathy,with 1.2g/kg Sanchi,0.6g/kg,and 100mg/kg Tiopronin,respectively.All rats were sacrificed 12 hours following the last injection.Blood samples were obtained prior to euthanization.Blood-fat and serum lipoidase activity were measured using an automatic blood biochemical analyzer.Blood haluronic acid(HA),laminin(LN)were determined by ELISA.Histopathological changes of hepatic tissues(i.e.steatosiss,inflammation,and fibrosis)were assessed by microscopic examination of HE and Masson staining of the right lobe of livers.Results Compared with rats from the normal group(treated with ddH2O),rats in the control group have significantly higher steatosis,inflammation,liver fibrosis,and increased level of serum cholesterol,HDL-C,LDL-C,haluronic acid,laminin and AST/ALT activity,(P0.05).More importantly,treatment with high-dose,low-dose Sanchi,or Tiopronin resulted in less severe steatosis,inflammation,fibrosis in the liver,and reduced the levels of serum haluronic acid,laminin(P0.05).Conclusions(1)Daily dosing of alcohol,corn oil,and pyrozole for 14 weeks faithfully replicated the alcoholic hepatopathy symptoms observed in the ALD model,such as hepatic steatosis,immune cell infiltration,blood-fat disturbance,increase in serum AST/ALT activity,and liver fibrosis.(2)Sanchi can significantly alleviate the symptoms in the alcoholic hepatopathy rats including steatosis,improve hepatic function and fibrosis.

Objective To analyze the determinants of television viewing among people in China,and to provide basic information for developing intervention strategies.Methods The data of 66 601 subjects aged 6 years and over(male 32 254,female 34 347) on television viewing from 2002 China National Nutrition and Health Survey was used.Results The prevalence of watching TV 2h and over among Chinese residents was 60.6%.Female had a decreased likelihood of watching TV 2h and over(adjusted odds ratio,AOR: 0.78).Children aged 13～17 years had a decreased likelihood of watching TV 2 h and over(AOR: 0.71) compared with younger children,however,adults had an increased likelihood(AOR: 1.34 for 18~44 yr,1.16 for 45～59 yr,1.04 for 60 yr and over).Light intense occupations were associated with an increased likelihood of watching TV 2 h and over(AOR: 7.18).Higher family income had an increased likelihood of watching TV 2 h and over(AOR: 1.12,1.07).Highest education level was associated with a decreased likelihood of watching TV 2h and over(AOR: 0.91).Engaging in exercise was associated with a decreased likelihood of watching TV 2h and over(AOR: 0.74).Conclusion Age,gender,socioeconomic status and exercise can influence the time spending on TV among Chinese residents.

OBJECTIVE:To summarize general regularity and characteristics of amiodarone-induced pulmonary toxicity,and to provide reference for rational use of amiodarone and avoiding the occurrence of ADR. METHODS:Retrieved from CNKI,VIP and Wanfang database,individual case report literatures about amiodarone-induced pulmonary toxicity were collected during 1990-2016. The included cases were analyzed statistically. RESULTS:A total of 19 related literatures were collected,involving 20 cases of amiodarone-induced pulmonary toxicity. Among them,the patients older than 60 years old accounted for 75.0% with ratio of male to female 3:1. 75.0% patients had used medicine more than 1 month when pulmonary toxicity occurred. The dose of amiod-arone in 17 patients ranged 200-400 mg/d. Six patients died,accounting for 30.0%. CONCLUSIONS:Pulmonary toxicity induced by amiodarone may be related to patients'gender,age,dose and medication time. The mortality of it is in relative high level. Med-ical staff should pay attention to it,regularly monitor and process it timely.

[Objective] To observe the effects of regulating Qi and strengthening spleen on the patients of chronic ulcerative colitis.[Methods] 38 patients of chronic ulcerative colitis(UC)were randomized and divided into experiment group(20 patients)and control group(18 patients)according to the selected standard.The patients of experiment group were treated with complex prescription of regulating Qi and strengthening spleen,the patients of control group were treated with granules of Mesalazine.To observe the symptom of accumulated points and enteroscope appearance on two groups.[Results] The difference on the symptom of accumulated points of experiment group exceeded the control group(P0.05).[Conclusion] There is effect of regulating Qi and strengthening spleen on the patients of chronic UC.