1.Investigation about the use of antibiotics for type Ⅰ incision thyroid surgery
Chinese Journal of Primary Medicine and Pharmacy 2017;24(7):1012-1014
Objective To investigate the use of antibiotics for type Ⅰ incision thyroid surgery of our hospital,so as to provide guidance for reasonable clinical use of antibiotics.Methods A total of 149 discharged patients of Xinhua Hospital,who underwent the type Ⅰ incision thyroid surgery from Jun 2015 to Jun 2016,were enrolled in the study.There were 117 cases underwent unilateral thyroid surgery,and there were 32 cases underwent bilateral thyroid surgery.According to the operation condition,the application of antibiotics in perioperative period,the number of days in hospital,the total cost of hospitalization,the infection situation and so on were analyzed.Results There were 69 cases(58.97%) who were perioperative prophylactic use of antibiotics,of which unilateral surgery 117 cases,and there were 20 cases(62.50%) who were perioperative prophylactic use of antibiotics,of which bilateral surgery 32 cases.The hospitalization days and hospitalization cost of the antibiotics used group were higher than those of the antibiotics unused group.All the cases were well stage wound healing,and one case occurred nosocomial infection.Conclusion It is not worthy for the prophylactic use of antibiotics for the type Ⅰ clean incision thyroid surgery to prevent the nosocomial infection,furthermore,the hospitalization days and hospitalization cost of patients increased.
2.Effect of Xionggui Dripping Pills on Sensitivity of Baroreflex in Selective Sinoaortic Denervated Rats
Shuying GUO ; Wei LIU ; Huiqin YU
China Pharmacy 1991;0(06):-
OBJECTIVE:To observe the effect of Xionggui dripping pills on sensitivity of baroreflex in selective sinoaortic denervated rats.METHODS:The dynamic blood pressures were monitored in selective sinoaortic denervated rats in waking stait,and the changes of baroreflex senitivity(BRS)were measured by modified SU Smyth method.RESULTS:Xionggui drip_ ping pill could improve BRS in sinus denervated rats.CONCLUSION:Since Xionggui dripping pill rectifies autonomic nerve function,it can protect target organs from damage in cardiovascular diseases.
3.Progress of the regulation effect of ginsenosides on HPA axis.
Hui LI ; Shuying LIU ; Bing WANG
Acta Pharmaceutica Sinica 2014;49(5):569-75
Ginseng is a typical adaptogen which has resistance to various stresses. This effect is related to the hypothalamic-pituitary-adrenal (HPA) axis. As the main active ingredients, saponin has the similar structure to steroids. The regulation characteristics of ginseng saponin on the HPA axis are narrated from the aspects of total saponin and saponin monomers in this paper after the introduction of adaptation definition and HPA axis regulation mechanisms. Pharmacological effects of ginseng saponin and the regulation effect of HPA axis are summarized finally.
4.Changes of calcium currents of ventricular myocytes surviving from infarcted rabbit heart
Shuying QI ; Kunshen LIU ; Zhenshan HE
Chinese Journal of Practical Internal Medicine 2001;0(04):-
Objective To determine the changes of L-type calcium currents(I Ca-L ) of rabbit ischemic myocytes in peri-infarcted zone.Methods Rabbit AMI models were made by ligation of branches of left coronary artery. After 1 week and 2 months, single pericardial myocytes were isolated enzymatically from ischemic zone adjacent to infarcted area and the normal control cells from similar regions in normal rabbit hearts. I Ca-L were recorded by using patch clamp techniques in the whole cell configuration. Results Peak of I Ca-L density of ischemic cells from the peri-infarcted zone was significantly reduced in AMI onc week later ( 3 52 0 93 pA/pF, n=6) compared to control cells ( 5 58 1 53pA/pF,n=10), P0 05 The steady-state inactivation curves were shifted to the hyperpolarizing direction in ischemic cells,the half-maximal voltage dependence of inactivation(V 1/2 ) in ischemic cells was 25 9 7 0 mV in AMI 1W , 21 3 5 6mV in AMI 2M and 13 1 4 2mV in the control cells(compared with control group,P
5.Expression and clinical significance of activated signal transducer and activator of transcription 3 in human gastric cancer
Juyan WANG ; Lijun CHEN ; Shuying LIU
Chinese Journal of Primary Medicine and Pharmacy 2006;0(03):-
0.05).Expression of Stat3 mRNA in human gastric cancer was significantly higher than that in their adjacent noncancerous tissues(P
6.Analysis of peer-assisted learning on critical thinking ability of undergraduate nursing students
Shuying YIN ; Wenwen ZHANG ; Weidong LIU
Chinese Journal of Medical Education Research 2017;16(2):199-203
Objective To analyze the implementation effects of peer-assisted learning on critical thinking ability of undergraduate nursing students. Methods A total of 411 college nursing students were selected with 203 students of Class One, Class Three, and Class Five from Grade 2012 majoring in nursing were taken as the control group, 208 students of Class Two, Class Four, and Class Six as the experimental group. In the course of basic nursing practice, the control group was trained by the traditional practice, and the experimental group was trained by the way of peer mutual aid. Critical thinking disposition inventory Chinese version (CTDI-CV) was adopted to evaluate the level of critical thinking ability of the nursing students of both groups. SPSS 17.0 was used and the data of the two groups were compared by t test. Results The score of experimental group students' critical thinking ability and the score of 7 dimensions including their seeking truth, analysis ability, self-confidence, inquisitiveness, cognitive maturity, open mind and systematic ability were higher than those before the experiment (P<0.05). The total score of the experimental group students' critical thinking ability and the score of 5 dimensions including seeking truth , analysis ability, self-confidence, curiosity and cognitive maturity were higher than those of the control group (P<0.05). Conclusion Peer-assisted learning is beneficial to the improvement of critical thinking ability of nursing undergraduate students.
7.Effects of atorvastatin on expression of lysyl oxidase in myocardial tissue of rats with diabetic cardiomyopathy
Hui LIU ; Shuying ZHANG ; Jidong RONG
Chongqing Medicine 2017;46(9):1172-1174,1178
Objective To study the effects of atorvastatin on expression of lysyl oxidase(LOX) in myocardial tissue of rats with diabetic cardiomyopathy (DCM) and its mechanism.Methods Thirty DCM SD rats were randomly divided into 3 groups:DCM group,treatment group (atorvastain 2 mg · kg-1 · d-1 by gastric gavage) and β-aminopropionitrile group(β-aminopropionitrile 80 mg · kg-1 · d-1 by gastric gavage),10 cases in each group.Other 10 SD rats were selected as the control group.At the end of week 8,the rats were killed for extracting the myocardial tissue RNA and protein.Expression levels of LOX,MMP-2 and NF-κB mRNAs and proteins in myocardial tissue of DCM rats were measured by RT-PCR and Western blot.Results The expression levels of LOX,MMP-2 and NF-κB mRNAs and proteins in the DCM group were significantly higher than those in the control group (P<0.01),and compared with the DCM group,the expression of BAPN LOX,MMP-2 and NF-κB mRNA and proteins in the treatment group were significantly deceased (P<0.01).Conclusion Atorvastatin can reverse the expression of LOX in myocardial tissue of DCM rat,and then may regulate the expression of MMP-2 and NF-κB.
8.The Third-stage Community-based Rehabilitation on Hemiplegia after Stroke
Lichun YAO ; Junqing LIU ; Shuying ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2009;15(7):677-678
Objective To observe the effects of the third-stage community-based rehabilitation program on functional recovery of stroke patients with hemiplegia. Methods 158 stroke patients with hemiplegia were divided into rehabilitation group and control group. Besides normal medicine and first-stage institution-based rehabilitation similar with that of control, rehabilitation group received a standardized third-stage community-based rehabilitation. They were assessed with the National Institutes of Health Stroke Scale (NIHSS), Fugl-Meyer Assessment (FMA), Modified Barthel Index (MBI) and Modified Rankin Scale (MRS) before, 1 month and 6 months after treatment. Results The scores of the FMA and the MBI of the rehabilitation group were higher than that of the control group (P<0.01), while were lower of the NIHSS and MRS in the rehabilitation group than in the control group at the end of the sixth month (P<0.01). Conclusion The third-stage community-based rehabilitation may improve the recovery of the stroke patients with hemiplegia.
9.The changes of transforming growth factor-β1 in pulmonary fibrosis induced by paraquat in mice1
Xuesong DONG ; Shengye LIU ; Wei LIU ; Shuying LIU ; Zhi LIU
Chinese Journal of Emergency Medicine 2011;20(8):826-829
Objective To study the changes of transforming growth factor-β1(TGF-β1) in pulmonary fibrosis induced by paraquat (PQ) with immunohistochemistry method. MethodsA total of 58 C57BL/6J male mice were randomly (random number) divided into the experimental group and control group. Pulmonary fibrosis was induced by intra-peritoneal injection of PQ in dose of 10 mg/kg into the mice of experimental group (n = 48), while physiological saline was used instead in mice of control group (n = 10). The mice of experimental group were sacrificed 2, 5, 7 and 14 days after PQ poisoning and the mice of control group were sacrificed on the 7th day after saline administration. Lungs of mice were taken and histological changes in lungs were evaluated by haematoxylin-eosin stain, and TGF-β1 was determined with immunohistochemistry method. The integrated optical density (iOD) value of TGF-β1 was measured and analyzed. ResultsThe TGF-β1 was markedly increased in macrophages during the genesis of pulmonary fibrosis induced by PQ. As the course of fibrosis progressed, the positive staining of TGF-B1 was mainly seen in macrophages and neutrophil's cytoplasm. On the 14th day after PQ poisoning, TGF-β1-positive cells were also detected in the fibroblast and myo-fibroblast inside the fibroblastic foci. Compared with the control group, the iOD value of TGF-β1 increased in experimental group (P < 0. 01 ) and it gradually upgraded during the course of fibrosis. Conclusions The TGF-β1 significantly increased during the course of pulmonary fibrosis induced by PQ and played an important role in the pathogenesis of the disease.
10.Change of the content of chemical constituents and anti-oxidative action of the decoction of radix ginseng combined with Flos Lonicerae, radix Polygoni Multiflori and Radix Astragali
Qinqin DU ; Xui ZHANG ; Fengrui SONG ; Zhiqiang LIU ; Shuying LIU
Acta Pharmaceutica Sinica 2010;45(6):756-60
Ginsenosides in the decoction of Radix Ginseng, Radix Ginseng with Flos Lonicerae, Radix Polygoni Multiflori or Radix Astragali have been investigated by high performance liquid chromatography (HPLC) and electrospray ionization mass spectrometric method (ESI-MS). Change of the content of ginsenosides was nonlinear in diverse combinative proportion of Radix Ginseng with Flos Lonicerae, while the stripping of ginsenosides was promoted by a small amount of Radix Polygoni Multiflori. In the combinative decoction of Radix Ginseng with Radix Astragali, ginsenosides contents were increased compared to single decoction of Radix Ginseng. Besides, ferric reducing antioxidant power (FRAP) method was developed for determination of the total antioxidative activity of n-butanol and water-soluble extracts from the decoction. The experimental results showed that antioxidative activity was better in the combinative decoction than that in single decoction, and the FRAP values of n-butanol extract were also greater compared with that of water extract.