In this paper,the fatty acid compositions of fish oils were analyzed and ox-idative property was evaluated. The result showed that the fatty acid composition varied greatly with fish. The content of PUFA(w3) of caplin oil,herring oil,mackeral oil menhaden oil was 11. 3%, 14. 3%, 21. 0%and 27. 6% respectively; the content of SFA was 17. 52%, 21.2%, 24. 12%and31. 15%; the content of MUFA was 62. 46%, 52. 9%, 40. 04%and 26. 26%. The content of EPA and DHA was about 15%-35%in anchovy oil,especially the DHA content (18- 44%)of anchovy oil in China was higher than EPAC13. 66%) and low content of C20,i and C22,1 But the DHA content of anchovy oil in South Africa,South Chile , North of Chile,Califor. US and Peru was lower than EPA. The content of SFA of fish oil increased with the storage and the content of PUFA in reverse. Temperature is an important factor affecting oxidation of fish oil.

Objective To study the protective effects of anisodamine (ADM) on ventilator-induced lung injury (VILI) in rats. Methods After anesthesia was induced and tracheostomy was performed, 36 healthy male Sprague-Dawley rats were randomly assigned into three groups: control group (group A, VT=8ml/kg); injury group (group B), in which the animals received mechanical ventilation with large tidal volume (VT=40ml/kg); and ADM protective group (group C), in which the animals received the same amount of tidal volume and same respiratory rate with mechanical ventilation as in group B, but were pre-treated with ADM (15mg/kg?iV) at 24h, 12h, 0h time points before ventilation. Arterial blood gases were measured every one hour, and neutrophils in bronchoalveolor lavage fluid (BALF) were counted. Wet to dry (W/D) weight ratio of left lung was determined, and the content of TNF-? and IL-1? in BALF and blood, malondialdehyde (MDA), superoxide dismutase (SOD) levels in the pulmonary tissue and blood were measured respectively. The histopathological changes in pulmonary tissues in the three groups were compared. Results The oxygenation index (PaO2/FiO2) of the animals in group B was significantly lower than that in group A and C, and the BALF neutrophil count was remarkably increased in group B. W/D value of group B was significantly higher than that of group A and C. The levels of TNF-?, IL-1? in BALF were remarkably increased in group B , but decreased in group C . MDA levels in pulmonary tissue and blood in group C were lower than that of group B, but SOD level in group C was significantly higher than that of group B. Histopathologic findings demonstrated there were more neutrophil infiltration and destructive change in the alveolar wall in group B than in other groups. Conclusion ADM appears to have obvious protective effects on VILI through anti-inflammation and antioxidation.

Objective To study scientific use and management of orthopedics implants.Methods The management of the orthopedics implants admittance was carried out.According to the purchasing lists from Procurement Public Health Bureau,special person was arranged to get the implants received,checked,disinfected,inspected etc.The application form,checking list and using list were used to reinforce the management of the implants from preoperation to postoperation.Results The management was carried out two years ago.The lose rate of the orthopedics fixed material was 0,and there was no important malpractice.Conclusion The scientific and systematic management of the orthopedics implants not only guarantees the safety of surgical operation and reduces the medical treatment disputes,but also safeguards the operation legal rights between doctors and sufferers at the same time.

Objective To investigate the effect and mechanisms of radix salviae miliorrhizae (RSM)on acute lung injury (ALI)caused by inhalation of high concentration rocket liquid propellant unsymmetrical dimethylhydrazine (UDMH)and dinitrogen tetroxide (N_2O_4). Methods Forty-two rats were divided into three groups:the control group,the inhalation group and the inhalation plus treatment group (RSM group).The latter two groups were inhaled with UDMH 0.98 g/m3 for ten minutes and N_2O_4 0.19 g/m3 for ten minutes.After the inhalation,the rats in RSM group were treated with 9 g?kg -1 RSM intravenously immediately,and were injected into peritoneal cavity with RSM at a dose of 3 g?kg -1 at the 3 hours after the first injection.The rats in other group were treated with equivalent saline.All rats were killed after the observation for six hours.Lung wet to dry ratio (W/D),LDH and total protein in bronchoalveolar lavage fluid (BALF),the activities of superoxide dismutase (SOD)and glutathione peroxidase (GSH-Px)in lung tissue,and the malondialdehyde (MDA)of plasma were measured.Histopathology was observed.Results The lung W/D ratio,LDH and total protein in BALF,MDA of plasma were increased in the inhalation group,while the activities of SOD and GSH-Px in lung tissue were decreased.The histopathology of the inhalation groups rats showed that exudation within alveolar space and interstitial thicken of septa was prominent.In the RSM group,above index were improved,and the degree of lung injury in histopathology was relieved.The lung W/D were related to the activities of SOD and GSH-Px in lung tissue,correlation coefficient were-0.661(P

Objective To investigate the effects of polysaccharide from Porphyra on spleen cell function in immunosuppressive mice. Methods The polysaccharide at dosages 0.025, 0.050 and 0.150g?kg~ -1 was injected intraperitoneally for 7 days in the immunosuppressive mice induced by cyclophosphamide. On day 8, the splenic lymphocytic proliferation and activity of TNF were determined. Results The splenic lymphocytic proliferation and cytotoxicity of tumor necrosis factor (TNF) of the group treated with 0.150g?kg~ -1 of porphyra polysaccharide were higher than those of model group. Conclusion Polysaccharide from Porphyra could promote splenic lymphocytic proliferation and raise the level of TNF.

Objective To study the CT findings of struma ovarii(SO)and improve the understanding of SO imaging features. Methods CT images of 6 cases were retrospectively reviewed.CT plain scan was performed in 6 patients;CT enhancement scan was performed in 2 patients.Results All tumors were unilateral.On non-enhanced CT,the lesions presented as well-defined irregu-lar cystic-solid masses.The cystic portions presented as well-defined,multiple,various size,and there were entire cystic walls with smooth inner wall.Four tumors showed high attenuation lesions in the cyst portion of the mass on precontrast scans.The solid por-tions showed irregular tissue density,and were often distributed in the cysts.The tumors showed stippled calcification in solid por-tions and/or cystic wall in 4 cases.One tumor accompanied a great of ascites liquid.After contrast administration,the cystic por-tions showed no enhancement,and the cystic walls and the solid portions showed mild enhancement.Conclusion CT findings of SO have certain characteristics such as a cystic-solid and well-defined mass with calcification,high attenuation lesion on plain CT,and marked solid part enhancement on contrast CT.

Objective To investigate the safety and efficacy of transarterial chemoembolization (TACE) combined with implantation of irradiation IVC stent in treating hepatocellular carcinoma (HCC)complicated by inferior vena cava tumor thrombosis (IVCTT).Methods The clinical data of 61 consecutive patients with HCC complicated by IVCTT were retrospectively analyzed.Irradiation IVC stent was prepared by strapping 125I particles on the bare stent,and it was employed in 33 patients (group A).Bare stent was adopted in 28 patients (group B).Propensity score matching method was used to conduct randomized analysis of the original data in order to reduce the selection bias.The survival time,remission rate of symptom and procedure-related adverse events of both groups were calculated and the results were compared between the two groups.Results The incidence of adverse reactions was similar in the two groups,and symptomatic treatment with internal medicine was adopted.The survival time in group A was superior to that in group B.The median survival time in group A was (203.0±28.1) days,which was (93.0±24.3) days in group B (P=0.006).Propensity score matching (24 pairs in total) cohort analysis showed that the median survival time was (200±31) days in group A and (66±23) days in group B (P=0.019).The edema remission rates in group A and in group B were 97.0％ and 96.4％ respectively.Multiple factor analysis revealed that irradiation stent implantation and objective tumor response were independent factors predicting a good prognosis.Conclusion For the treatment of HCC associated with IVCTT,TACE combined with irradiation stent implantation is safe and effective,this therapy can prolong the patient's survival time

Objective To study the nuclear protein association of high-mobility group box-1 (HMGB1) and histone deacetylase 1 (HDAC1),and the effect of interaction on radiosensitivity in human breast cancer cells.Methods The protein-protein interaction was determined by immunoprecipitationWestern blot and glutathione-S-transferase capture assays.Cell growth was examined by MTT (methyl thiazolyl tetrazolium)assay and clonogenic assay.Histone deacetylase activity was analyzed by histone deacetylase assay.Results A significant increase of HMGB1 protein and radiosensitivity was observed in MDA-MB-231 and MDA-MB-468 cells transfected with a pCMV-Tag2B expression vector carrying with a full-length of HMGB1 cDNA.HMGB1 binding to HDAC1 was demonstrated as GST (glutathione Stransferase)-pull down and immunoprecipitation Western blot assay,and the association was elevated by irradiation.An LXCXE motif was required for the HMGB1-HADC1 interaction and HMGB1 radiosensitization.A significant difference of IC50 value was observed,for example,1.8 and 2.2 Gy (wtHMGB1 transfectants,P ＜ 0.05),3.6 and 3.8 Gy (HMGB1/C103F transfectants,P ＞ 0.05),both compared with 3.9 and 4.1 Gy (pCMV-Tag2B transfectants) in MDA-MB-231 and MDA-MB-468 cells,respectively.A specific HDAC1 inhibitor trichostatin A markedly reduced the HMGB1-mediated radiosensitivity,0.5 Gy in the presence of trichostatin A versus 1.8 Gy in absence of trichostatin A in MDA-MB-231 transfectants,1.2 Gy (with trichostatin A) versus 2.2 Gy (without trichostatin A) in MDA-MB-468 transfectants,P ＜ 0.05.Histone deacetylase activity was also detected in immunoprecipitates prepared from these cells with antibodies to HMGB1,and this activity was abolished by the histone trichostatin A.Conclusions These results suggest a previous unanticipated role for HDAC1 in modification of HMGB1-mediated radiosensitivity by its direct interaction with HMGB1.

Objective To analyze the clinical factors predicting long-term survival after curative resection of pancreatic head carcinoma. Methods The clinical data of 58 patients with ductal adenocarcinoma of pancreatic head who underwent curative resection of carcinoma of pancreatic head from 1996 to 2004 were collected and were analyzed by SPSS 10.0 with Cox Proportional Hazards Model. Results 58 patients, including 30 male and 28 female patients, were involved in this study. Pancreaticoduodenectomy were performed in 14 cases and extended resections were performed in 44 cases. The overall 1, 3, 5 year survival rates was 46.6%, 29.3% and 8.6%, respeclively. The 1,3, 5 year survival rates of pancreatoduodenectomy with extended regional lymphadenectomy was 43.1%, 22.7% and 6.8%, respectively. UICC staging, peri-pancreatic nerve invasion and blood infusion had significant effects on the prognosis after curative resection. Conclusions The long-term prognosis after curative resection of pancreatoduodenectomy was still dismal. Much importance should be paid to early diagnosis and comprehensive management for pancreatic head cancer.

Objective To evaluate the correlation between regional blood perfusion,metabolism and angiogenesis in breast cancer.Methods The PET/CT functional imaging technique was applied to quantitatively detect the central and marginal blood perfusion parameters including blood flow(BF),blood volume(BV)and permeability of surface(Ps),and metabolism index of standard uptake value(SUV)of the tumor in 33 breast cancer patients.The expression of CD31.CD105 and VEGF in paraffin section of breast cancer were detected by immunohistochemical method,then MVD(CD31)and MVD(CD105)were obtained.The relationship between the regional blood perfusion and metabolism and MVD(CD3 1),MVD(CD105)and the expression of VEGF were analyzed. Results There was significant correlation of MVD (CD31)with BF of marginal region(P<0.05).There were significant correlations of MVD(CD105)with BF.PS and SUV(P<0.05). Conclusions Regional blood perfusion,metabolism is correlated with angiogenesis in breast cancer tissue.PETT/CT regional blood perfusion and metabolic imaging iS a noninvasive method which can be used to estimate angiogenesis status clinically in breast cancer.