Triple negative breast cancer(TNBC) is defined by the lack of estrogen receptor (ER) , progesterone receptor (PR) and human epidermal growth factor receptor-2 ( Her-2) expression. It is a subtype of tumor with distinct clinical and pathologic features and lacks effective targeted therapies. It is non - sensitive to endocrine therapy and targeted therapy drug herceptin, leaving chemotherapy as the mainstay of treatment The feature and the treatment of TNBC is the hotspot of resent research.

Glycogen storage diseases (GSD) are a group of inherited disorders characterized by enzyme defects that affect the glycogen metabolism. Hypoglycemia, hepatomegaly, splenomegaly and growth retardation are the main clinical manifestations. The enzymes affecting glycogen synthesis and degradation are varied.Consequently, the clinical manifestations are different because of different enzyme defection. Most patients treated regularly can obtain the normal growth and grow to adults, but still can be accompanied long-term complications by adulthood. Now some scientists find new modified cornstarch is superior to standard therapy in maintaining blood glucose levels. Gene therapy is still in the experimental animals.

C-termini of proteins often play an important role in various biological processes, such as the transcription and translation from DNA to protein and also participating in various biological regulations. The determination of protein C-terminus is so crucial because it provides not only distinct functional annotation, but also a way to monitor the proteolysis-modified proteins. Based on the biological mass spectrometry, a series of novel methods and technologies were developed both for qualitative and quantitative analyses of protein C-terminus. These methods or technologies can be applied to accurate and effective protein C-terminus profiling, including the sequences and quantitative information of C-termini, which reveals the biological function of C-termini in life's activities and provides a better understanding of the degradation of mature proteins. Combined with our research, this review highlights the improvements in C-terminal proteomics study in the past decades, including the methodologies for recognition and identification of C-terminus, as well as the enrichment strategies for protein C-terminus.
Mass Spectrometry ; Protein Processing, Post-Translational ; Proteins ; chemistry ; Proteolysis ; Proteomics

Recent studies have shown that pituitary hormones, including thyroid stimulating hormone (TSH), follicle stimulating hormone(FSH), adrenocorticotropic hormone(ACTH), and oxytocin(OT)may actually bypass their target endocrine organs and affect the skeleton directly. Therefore, a new conception, pituitary-bone axis is proposed. This breakthrough sheds a new light on the function of pituitary hormones and the pathogenesis of osteoporosis associated with hyperthyroidism, menopause or pregnancy, and even osteonecrosis after using glucocorticoids. In addition, it is conducive to give the reference guidance for clinical treatment of metabolic bone diseases and new drug development.

BACKGROUND:For reducing the stress shielding, a lot of the schemes have adopted, and there is no great improvement although via the trial and the application for several ten years. It comes from a root which is the combination by thigh bone (abbreviation of the bone) and metal ic handle (abbreviation of the handle) which causes the serious problems of absorption of bone quality, and this kind of stress shielding effect is produced by abnormal stress and abnormal strain, and affected by various factors, so there is no effective and applicable method to solve this problem.
OBJECTIVE:To select the primary porous layer scheme as the subject of the study and entirely change the appearance and inherent quality as the metal ic handle that cannot transmit the stress is the key to eliminate the stress shielding;making the senior porous layer with the effective measure is the basis to eliminate stress shedding and can solve the disaster and partial inferior position aroused by the primary porous layer.
METHODS:The cobalt-chromium-molybdenum al oy smal metal ic spherules with equal diameter were used to manufacture the senior porous layer structure. The structural member employed in the thigh bone could separate the handle from the bone. This structural member welded on the plane of bone neck could fix the handle with calcar osteotomy surface. And these structural members had an ability to eliminate al the stress shieldings on the artificial hip joint surface.
RESULTS AND CONCLUSION:The senior porous layer was made with cobalt-chromium-molybdenum al oy smal metal ic spherules with the same diameter (1.00-1.29 mm), and the surface of the porous layer was plated with high property of medium layer al oy and arranged orderly and inseparable, and then connected with active diffusion welding technology. The diameter of al the holes was 155-200μm and distributed evenly which was benefit for the bone unit to grew. The al oying of metal materials, ceramic of al oy surface and control ing of size and number of wear particles can solve the bone dissolution of the surrounding porous layer of bone, metal dissolution as wel as its pure cobalt and chromium particle toxicity, cancerization and leukemia. A stipulation of theoretical basis was to eliminate the handle rigidity firstly that could made the separation of handle from the bone became the premise for eliminating the stress shielding. The calcar osteotomy surface grew into the pores of the structural member welded on plane of bone neck could fix the handle, and that was the basis for eliminating the stress shielding. The common roles can eliminate the stress shielding in thigh bone, and the stress shielding on acetabulum is easily to eliminate.

To investigate the correlation between traditional Chinese medicine (TCM) syndrome and short-term prognosis of ischemic stroke.

The famous Abraham Flexner's 1910 report to the Carnegie Foundation launched the modern medical educmion.The report'S focus on the medical education standards and the curricula frameworks was laudable and appropriate.At the centennial of this report.we were still able to get a lot of inspiration and the direction of development and reforms of medical education by reviewing Flexner'd Report.

Objective To investigate the relationship between body mass index(BMI) and the outcomes of liver function of patients with hepatocarcinoma after surgery. Methods We divided the patients into three groups: normal, overweight and obese groups based on BMI, then we recorded their preoperative and the postoperative liver functions: albumin, total bilirubin, alanine aminotrasferase(ALT), aspertate aminotransferase (AST), alkaline phosphatase(ALP), γ-glutamyltranoferase(γ-GGT), triglycerides(TG), total cholesterol (TC), high density lipoprotein(HDL) and low density lipoprotein(LDL). Results The mean values of ALT change between the preoperative and the postoperative were (14.9 ± 1.98) U/L, (15.4 ± 2.27) U/L,(22.2±1.32) U/L; Mean values of γ-GGT change were(16.2 ± 1.33) U/L, (18.7 ±0. 97) U/L,(22.3 ± 1.27) U/L; mean values of TG change were (0. 18 ± 0. 44) mg/dL, (0. 21 ± 0.08) mg/dL,(0.25 ± 0.03) mg/dL. Compared with the results of control groups, there were no significant differences of recovery of liver functions between normal and overweight groups(P ＞ 0. 05), but the obese group' s recovery of liver functions did lag behind the normal group (P ＜ 0.05). It is showed that the obese group' s shared same recovery of albumin, total bilirubin, AST, ALP, TC, HDL and LDL. The difference was significant between the normal and obese groups in terms of recovery of ALT, γ-GGT, TG(P ＜ 0. 05). Conclusion BMI is associated with the recovery of liver function of patients with hepatocarcinoma, the obese patients have poor prognosis of recovery of liver function.

Objective To study the mechanism of toxicity of hexane. Methods SD rats inhaled 15 g/ma n-hexane statically for 8 hours. Results The levels of GSH in whole blood of rats declined significantly (t-test,P＜0. 01). The levels of MDA in serum of rats revealed increasing trend but without statistical significance (t-test,P＞0. 05). The activities of SOD and GSH-Px in liver of rats decreased significantly (t-test,P＜0.01). Conclusion n-Hexane could induce or enhance the reaction of oxygen free radical in organism,and result in damages of lipid peroxidation,which might be one of the mechanisms of the toxicity of alkane.

[ABSTRACT]OBJECTIVETo evaluate the efficacy of combined oral and intratympanic glucocorticoids treatment for severe to profound sudden sensorineural hearing loss(SSNHL).METHODSFifty-four patients with severe to profound SSNHL were retrospectively studied. They were treated with oral steroids, vasodilators, anticoagulants. The patients who accepted intratympanic methylprednisolone sodium succinate were included the therapy group, the others were included in the control group. Hearing improvements were analyzed respectively after two weeks.RESULTSHearing in both groups were improved significantly after treatment(P<0.01), but there was no difference in hearing improvements between two groups(P=0.194). The efficacy for severe SSNHL patients in therapy group was equal to those who were in control group (P=0.251), so was the efficacy for profound SSNHL patients in both groups(P=0.380).CONCLUSIONThe combined systemic and intratympanic glucocorticoids treatment was effective for patients with severe to profound SSNHL, but while with no better efficacy than oral glucocorticoid alone.