0.05). In comparison with the control group,the levels of FPG and GSP were significantly higher (P

Objective To study the action of GSK-3βin podocyte transdifferentiation effecundethe high glucose condition . MethodPodocytewere treated in RPMI-1640 medium with differenconcentrationof glucose fo36 h .The expressionof neph-rin ,podocin ,α-Smand Fibronectin were detected by both Western bloand indirecimmunofluorescence analysi.Athe same time ,the change of albumin inflow amounof podocytein differentreatmengroupwadetected by using the Transwell cham-be.The changeof expression amounand activity of GSK-3βin high glucose condition were detected by using the GSK-3 activity assay ki.The phenotypiand functional changeof podocytewere detected aftedisturbing GSK-3βby GSK-3βsiRNin the high glucose group .ResultThe expression levelof nephrin and podocin protein were down-regulated with the increase of the glucose concentration in dose-dependenmanner(P＜0 .05) ,and the expression level of α-Smwaup-regulated with the increase of the glucose concentration in dose-dependenmanner(P＜0 .05);the albumin inflow amounof podocytewaup-regulated with the in-crease of the glucose concentration in dose-dependenmanner(P＜0 .05) .The expression amounof GSK-3βin the high glucose group waincreased(P＜0 .05) .Compared with the control group ,the expression amounof nephrin and podocin aftehigh glucose treatmenin the GSK-3β siRNtreatmengroup waincreased ,while the expression of α-Smwadecreased .Conclusion The high glucose condition could induce the phenotypichange and functional impairmenof mice podocyte;GSK-3βparticipatein the epithelial-mesenchymal transdifferentiation procesof podocyte undethe high glucose condition .

The morbidity of young non-small cell lung cancer(NSCLC)increases in the recent years.Young group patients should be defined as age under 40 years old.The risk factors of etiology in the young NSCLC may be related with smoking,genetic predisposition,Internal and external environment factors havoc and etc.There is no specific symptoms of young NSCLC,therefor,it happens misdiagnosis and harder to be confirmed.The morbidity in young female group of NSCLC patients is higher than aged patients.The most frequent histopathologic type is adenocarcinoma and the ratio of advanced stages with low differentiation has been found to be more common.The chances of surgery seems lower than others.EGFR gene mutation percentage in the young NSCLC maybe lower than that in the old patients,while the positive mutation of MEL4-ALK gene maybe more prevalent in the young patients.The prognosis of young NSCLC seems to be poor with lower tumor progression time of the first line therapy.Better prognosis of young NSCLC patients maybe achieved by early diagnosis and efficient therapy according to the accurate genotypes.

Objective To investigate the protein and mRNA expression of NF-κB and zinc finger protein A20 in the keloid and normal skin and to explore its underlying molecular biological mechanism.Methods The protein and mRNA expression of NF-κB p65 and zinc finger protein A20 in keloid and normal skin was detected by immunohistochemistry and real time quantitative reverse transcription-polymerase chain reaction (RT-PCR).Results The protein and mRNA expression of NF-κB p65 in keloid tissues was higher than those in normal skin tissues (P＜0.05).The protein and mRNA expression of zinc finger protein A20 in keloid tissues was lower than those in normal skin tissues (P＜0.05).Conclusions NF-κB signal pathways and zinc finger protein A20 could play a role in keloid pathogenesis.

Objective To observe the anesthetic effects of articaine hydrochloride and epinephrine tartrate injection in oral treatment of elder patients.Methods 66 teeth of 56 elder patients were treated.Pain intensity was scaled by VAS.Pulse and blood pressure were observed before and after drugs administration for 0,5 minutes.ResuIts Pain during injection of articaine hydrochloride and epinephrine tartrate injection and surgery subsequently was rare.The total respouse rate was 95%～100%.No significent toxic effects were found.Pulse and blood pressure remained after injection.Conclusion Articaine hydrochloride and epinephrine tartrate injection was one of local anesthetics with good infiltration,safety and anesehetic effect.It would be more widely used in elder patients oral treatment field in the future.

Objective To evaluate the clinical effects of magnetic attachments in complete overdenture. Methods Casting method was used to fabricate Coping-Keepers for remained roots, and the patients were followed up for 6 months to check the effects of the overdentures retained with Magfit EX attachments. In the experimental group, 21 complete overdentures were made with 33 Magfit EX magnetic attachments for 18 patients. Eleven patients treated with traditional complete overdentures were selected to act as control. The retention force, aspect and comfortableness were subjectively evaluated by the patients of the both groups. Abutment status was observed and compared before and after cementation of the magnetic attachments by measuring periodontal index, including gingival index (GI), sulcus bleeding index (SBI) and bleeding on probing (BOP), etc. Masticatory function of patients in the both groups was measured at 3 time points: immediate, 3 months and 6 months after cementation. Results Patients in experimental group were more satisfied with complete overdentures made up of magnetic attachments than those in control group, especially in retention force and masticatory efficiency and aspect. Magnetic attachments can provide enough retention force for patients and greatly improved the masticatory efficiency and the abutment condition. The masticatory function was to be perfect and stable after 3-6 months. Furthermore, the mobility and periodontal index of most abutments improved after 6 months. Conclusion It is proved that magnetic attachments are effective to improve the function of complete overdentures, worthy to be used widely. It is suggested that the residual root and loosened tooth should be preserved furthest as possible in clinic.

0.05).The differences between NSCLC and SCLC reached the statistical signifcance,P