Objective:To explore the effects of 1,25-dihydroxyvitamin D3 on renal expression of TGF-?1 and HGF in type 1 diabetic rats.Methods:30 Sprague-Dawley rats were randomized into 3 groups:normal control rats(group NC),diabetic rats(group DM ) and diabetic rats treated with 1,25-dihydroxyvitamin D3(group DC).Kidney-to-body weight ratio(KW/BW),mean glomerular volume(MGV),fractional mesangial area(FMA),urinary albumin excretion rate(UAER)and serum creatinine(Scr)were measured after 12 weeks of treatment,while transforming growth factor-betal(TGF-?1) expression in kidney cortex were observed by immunohistochemistry.The expression levels of TGF-?1 mRNA and HGF mRNA were detected by reverse transcription and polymerase chain reaction(RT-PCR) at the end of the experiment.Results:UAER,Scr,MGV,FMA and KW/BW ratio were significantly higher in the diabetic rats than those in the normal control rats(P

Objective To study the clinical characteristics of abnormal lipid metabolism in different types of elderly people with hypertension.Methods The clinical data,collected from the elderly people who underwent annual physical examination,the characteristics of dyslipidemia and abnormal lipid metabolism in different types of elderly people with hypertension were analyzed retrospectively. Results The prevalence of dyslipidemia was 69. 63％, in which the prevalence of hypercholesteremia and hypertriglyceridemia was 23. 65％, and 26. 71％ respectively. The prevalence of hypercholesteremia accompanied hypertriglyceridemia( mixed group) was 19. 27％, and normal group was 30. 37％. Hypertriglyceridemia group was easier to be accompanied with hypertension than normal group and HTC group(P ＜0. 05). The hypertriglyceridemia had higher risk of cardiovascular and cerebrovascular diseases than norral group and HTC group( P ＜ 0. 05 ). Conclusion The elderly people had high prevalence of dyslipidemia and metabolic syndrome,and with high prevalence of hypertension, which led to higher risk of cardiovascular and cerebrovascular diseases. The hypertriglyceridemia had more strong impact on cardiovascular and cerebrovascular diseases than hypercholesteremia.

Long non-coding RNA (lncRNA) is a group of functional RNA molecules, which is more than 200 nu-cleotides in length, lacks ability of encoding protein and participates in all aspects of gene regulation. The current studies have indicated that some lncRNAs take part in regulating the process of breast cancer tumorigenesis and progression. This article reviewed the abnormal expression of lncRNA in breast cancer, its relationship to breast cancer growth, apoptosis, invasion and metastasis, and its function in early diagnosis, drug resistance and prognosis of breast cancer. This review was expected to offer an insight into a new target for the prediction, diagnosis and individual treatment of breast cancer.

Objective Compared with surgical operation,to investigate the clinical efficacy and advantages of interventional treatment in blunt liver traum.Methods 32 patients from July 2010 to October 2012,who were diagnosed as blunt liver trauma,received super selective hepatic artery embolization in Chinese People's Liberation Army in 98th Hospital.Another 27 ones received operation at the same time in Peolies's Liberation Army 98th (AAST grade Ⅱ to Ⅳ).Retrospective analysis of related cases was presented.The results were analyzed by t test.Results All of 32 patients that received super selective hepatic artery embolization were stanched bleeding successfully.All patients were followed up for 3 to 18 months,the mean follow-up time was 10 months,with no severe complications occurred or no lost cases.Conclusions Compared with surgical operation,interventional treatment had advantage that included eliable hemostasis,less operation time,minimally invasive and less hospitalization time.Interventional treatment had good effects on blunt liver trauma which were AAST grade Ⅱ to Ⅲ.

AIM: To study the effect of herbal compound poria prescription (CPP) on proliferation and differentiation in primary rat preadipocytes, and to explore the mechanism of this process. METHODS: Primary cultured rat preadipocyte proliferation was determined by cell counting and MTT spectrophotometry. Cell differentiation was determined by Oil Red O staining, and the expression of PPAR? mRNA was determined by RT-PCR. RESULTS: CPP inhibited the proliferation and differentiation of primary rat preadipocytes in a dose-dependent manner, and it also inhibited the expression of PPAR? mRNA. CONCLUSION: CPP inhibits the proliferation of primary rat preadipocytes and inhibits the preadipocyte differentiation by suppressing the expression of PPAR? mRNA.

Severe acute pancreatitis is a relatively common surgical disease. Patients have a hypermeta-bolic state and nutritional support for patients with SAP is needed. Early enteral nutrition support is a viable option and can improve the nutritional status of patients, protect the gut barrier function, reduce bacteria translocation to regulate the inflammatory response and reduce hospitalization costs. Combined with our do-mestic and foreign literatures in recent years, this review the feasibility and practical implementation of enter-al nutrition in severe acute pancreatitis treatment summarizs.

Objective:To induce experimental autoimmune uveoretinitis in Wistar for investigating the pathogenesis mechanism of uveitis.Methods:18 Wistar rats were immunized by different dosage of bovine soluble antigen(S Ag),and were detected the clinic changes dayly.The animals were anesthetized and killed when they had significant clinic EAU appearance or 3～4 weeks after immunized,the eyes were removed and followed by pathologic studies.Results:The incidence of EAU in the 3 different dosage S Ag immunized rats were 2/12 in 100 ?g S Ag group(6 rats,12 eyes),6/12 in 200 ?g S Ag group(6 rats,12 eyes) and 8/12 in 300 ?g S Ag group(6 rats,12 eyes) separately and the pathology scores were 0?16 76?11.02?17 56?9 96 separately in 3 group of rats.Conclusion:The middling sensitive Wistar rats can be used to induce EAU successly with middling purified S Ag.

Tear fluid is a complex body fluid,which may contain thousands of protein/peptides and other molecules.Studies have determined that the changes in the chemical compositions of tears play an important role in some diseases and their progression.Tear fluid is a useful and easily accessible source for understanding ocular surface diseases,other eye diseases,and systemic diseases.It can also be used for identification of biomarkers for clinical applications and pharmaceutical development.Therefore,quantitative proteomic analysis of tears may provide very important information for diagnosis and treatment of eye diseases or the development of new drugs.Knowledge of the current proteomic technologies for tear analysis is helpful for conducting studies.In addition,ophthalmologists should pay close attentions to the association between tear proteomic changes and eye diseases,recent advances in tear proteomics and their applications in studying ocular surface diseases and conditions.

Previous research demonstrated that human tear is a valuable source for biomarker discovery for many ocular surface diseases.Here, we systematically summarized the changes of tear protein profile in many ocular surface diseases, including dry eye, blepharitis, ocular allergy, keratoconus, infectious keratitis, conjunctivochalasis, ocular rosacea, and thyroid-associated ophthalmopathy and other conditions, such as diabetes, ocular surface wounding and refractive surgeries, contact lens wearing, and effects of glaucoma medication on ocular surface, attempting to make ophthalmologists understand the specific changes of tear protein profile in each disease or condition and hold the promise for optimal management of the diseases.

Choroidal neovascularization( CNV) is a common severe complication of ocular fundus diseases, with great potentiality to cause blindness. The treatments of CNV include photodynamic therapy ( PDT ) and anti-vascular endothelial growth factor( VEGF) therapy. However, PDT produces little visual benefits, and may result in ischemia, inflammatory responses, even the secondary expressions of VEGF. Anti-VEGF therapy sustained visual efficiency by repeated injections, causing economic burden and adverse effects related to injection. Recently, PDT combined with anti-VEGF therapy has progressed in laboratory and clinical research. Therefore, the combined modality therapy became one of the treatment options for CNV. In order to reduce the side effects of mono -therapy, further study is required to determine appropriate combinations and dosage.