Objective To explore the effect of simple active respiratory training method on lung function for cervical spinal cord injury. Methods 24 male patients with cervical spinal cord injury were divided into two groups, the control group (n=14) and the treatment group (n=10). 2 groups all received the same rehabilitation training, while the treatment group was added simple active respiratory training method. Lung function was assessed after eight-week intervention. Results 2 groups improved in percentage of expected value of maximum vital capacity (VCmax), forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), peak expiratory flow (PEF), and maximal voluntary ventilation (MVV) 8 weeks after the treatment(P<0.05), while the treatment group was better than the control group(P<0.05). Conclusion Rehabilitation training can improve lung function of the patients with cervical spinal cord injury, and simple active respiratory training method has better effect.

Objective To evaluate the efficacy and safety of cellular bone allograft(CBA)in ankle arthrodesis.Methods The clinical data of 56 patients with traumatic ankle joint injury and ankle joint injury treated in our hospital from April 2013 to April 2016 were retrospectively analyzed.Imaging was performed at 3 months after surgery,and the visual analogue(VAS)score of the patients,the ankle and foot surgery score(AOFAS),and the SF-36 health status questionnaire score were recorded.The group included 33 patients who were treated with CBA,and patients with simple fusion in the treatment group(n=23).Results 2 months after operation,the fusion rate of CBA group was 87.9% and the fusion rate of without CBA group was 52.2%.3 months after operation,the fusion rate of CBA group was not higher than that of group CBA,and the fusion rate was in group.The data showed a significant improvement in pain,function,and quality of life.No adverse events caused by CBA were observed during the study.Conclusion The rate of fusion was higher in patients treated with bone allograft(CBA)than in patients with autologous bone graft,and the fusion rate was not affected by high risk factors.CBA is a safe and effective graft material for the bone healing of damaged joints,which can provide an effective bone graft substitute for ankle arthrodesis.

Objective To explore the clinical effects of therapeutic methods on child distal fracture of radius.Methods Totally 180 children with distal 212 fractures of radius from January 2012 to December 2015 in some hospital were enrolled into three treatment groups and a control group,of whom,there were 58 ones in a treatment group with external fixation support (external fixation group),58 ones in a treatment group with T-shaped plate internal support (T-shaped plate group),56 ones in a treatment group with Kirschner wire internal fixation (Kirschner wire group) as well as 40 ones in the control group with manual reduction and plaster external fixation.The clinical effects in the four groups were observed,and function scoring was carried out according to Dienst standard.Results The excellent rates in the external fixation group,T-shaped plate group,Kirschner wire group and control group were 72.41％,53.57％,82.76％ and 45％ respectively.The external fixation group had the excellent rate with no significant difference with that in T-shaped plate group (P＞0.05),while obviously higher than that in Kirschner wire group (P＜0.05),and Kirschner wire group had the excellent rate statisticallyhigher than that in the control group (P＜0.05).The external fixation group,T-shaped plate group and Kirschner wire group had the satisfaction significantly higher than that in the control group (P＜0.05).Conclusion Compared with the traditional reduction and Kirschner wire fixation,the external fixation and T-shaped plate fixation have better clinical effects.The treatment of child distal fracture of radius should be planed according to the type of fracture,stability,age,economic condition and substance of the bones of the patient.

Objective To evaluate the global contractile function of the left ventricle (LV) by speckle tracking imaging (STI) in patients with myocardial infarction (MI), in order to determine the feasibility of evaluation of LV funtion with mitral annular displacement (MAD).Methods The systolis parameters including longitudinal strain (GLs) ,circumferential strain (GCs) and MAD were obtained using STI and LV ejection fraction (LVEF) was acquired by three-dimensional echocardiography in control group and MI group,and the correlation between these parameters and LVEF was analyzed respectively.Results The parameters in MI group were significantly lower than those in the control group(P ＜0.01).GLs was the independent predictors of LVEF in control group and MI group.A good correlation was showed between MADin control group and MI group (r = 0.84, P ＜0.01 and r = 0.87, P ＜0.01, respectively).Conclusions MAD may be a promising modality to evaluate the global systolic function of LV in patients with MI for clinical routine practice.

The article presents an analysis of clinical signs, pathological findings and results of laboratory examinations of 10 cases of insulinoma. Diagnostic procedures such as blood sugar and serum insulin determinations, elective arteriography and CT scanning are evaluated. Besides, the relationship between the clinical signs and pathological findings were studied. The results of the study showed that the ratio of blood sugar to insulin and elective ateriography were valuable significant in diagnosing insulinoma, and that the diagnostic efficiency of CT left much to be desired. They also showed that duration of the disease had remarkable effects on patients with insulinoma.

The selection of appropriate candidates for uterine artery embolization with ultrasound is the key for obtaining accurate assessment and planning of the proper course for the proceduce. The patients should be followed-up after uterine artery embolization by ultrasound examination at different periods for the assessments of volume, echogenicity and vascularization of the fibroids as well as the uterine blood supply. After all, ultrasound gives important information especially concerning the vascularity of uterine leiomyomas before and after uterine artery embolization. Also it is a non-invasive and economic method in the determination of the efficacy in the early and late periods after the procedure. (J Intervent Radiol, 2006, 15: 568-570)

Objective To investigate the effects of biological characters of the human ovarian cell line (OVCAR) by stable transfection short hairpin RNA into the target HMGA1 gene. Methods Experiments were divided into two groups: transfected the OVCAR cells with pSilence4. 1-CMV-Hs plasmid as group A, while transfected OVCAR cells with pSilence4. 1-CMV-Hn plasmid as group B, in which stably transfected cells were gained by antibiotic screening. The comparative expressions of HMGA1 were detected by RT-PCR and western blot. Methyl thiazolyl tetrazolium (MTT) method was applied to measure cell proliferation and at the same time, the cell growth curve was also be mapped. Vitro invasion assay was used to observe the invasion ability of the cancer cells, and the tumor growth of the nude mice inoculated of tumor cells were compared with before and after transfection. Results In group A, the expression level of mRNA and protein HMGA1 gene in OVCAR cells were remarkably reduced before and after the stable transfected with HMGA1 siRNA, in which the percents of mRNA expression were [(86.3±2.7) % vs. (35.8± 3.1) %, P<0.05], the expression of protein were [(68.6±2.8) % vs. (22.3±4.2) %, P<0.05)]. The OVCAR cell growth in stable transfection status was more significantly decreased than that in non-transfection status (P<0.05). In group B, there were no statistical difference in the expression of HMGA1 siRNA, protein and the cell growth between before and after transfection states (P>0.05). The invasion cell numbers were reduced from before to after transfection state in group A [(53±6)vs. (21±6), P< 0.05] ,while there was no significant difference in group B [(51±6)vs. (47±8), P>0.05]. After inoculated transfected cells into nude mice, it took (6.0±0.9) days to grow the planed tumors in group A, which was much shorter than that (12.3±3.9) days in group B (P<0.05 ). After 5 weeks, the tumor weight and volume in group A was were significantly lower than those in group B [(0.8±0.3)g vs. (2.1± 0.4)g, (205±34)mm~3 vs. (987±82) mm~3, all P <0.05]. Conclusion HMGA1 siRNA could remarkably reduce the expression of HMGA1 gene in ovarian cell and also inhabit the ovarian cell growth.