Objective To investigate the surface sterilization and modification of medical poly(tetrafluoroethylene)(PTFE) by remote argon plasma.Methods Carrier quantitative bactericidal test was used to examine the germicidal effect(GE) of the E.coli on the surface of medical PTFE film and surface structure,performances of sterilized PTFE were characterized by the water contact angle,mass loss and X-ray photoelectron spectroscopy(XPS).Results Both remote and conventional argon plasmas could sterilize effectively(GE≥3.769) in the conditions of plasma RF power 100 W,exposure time 120 s and argon flux 20 cm3/min.Remote argon plasma sterilization made the PTFE surface have higher hydrophilicity(?=58.5?) than the conventional argon plasma sterilization(?=70.5?) and did not cause remarkable degradation and damages in terms of the mass loss(mass loss was 11.8%).Remote argon plasma sterilization could enhance interaction reactions with argon radicals relative to those with electron and argon ions,thereby contributing more effectively to defluorination(F/C=2.24) from the PTFE than the conventional argon plasma sterilization(F/C=2.49) and more effectively to the formation of oxygen functionalities on the PTFE surface.Conclusion Remote argon plasma can inactivate E.coli effectively and at the same time bring better surface modification of PTFE.

Objective To explore the coping styles and family environment in patients with depression.Methods 90 patients with depression were evaluated with CSQ and FES-CV.Results There were significant differences in the family environment between depression group and normal control group(P＜0.01).The factor scores of seeking-help and solving question in depression group positively correlated with the factor scores of family intimacy and success(r=0.29,0.32,0.27,0.31,P＜0.05).The factor score of self-accusation in depression group negatively correlated with the factor score of affection expression(r=-0.27,P＜0.05).Conclusion The coping styles are associated to family environment in patients with depression.

Objective To observe the clinical curative effect of Tanreqing injection of antibiotics in the treatment of pulmonary infection in the elderly.Methods 120 cases of elderly patients with pulmonary infection from June 2015 to June 2016 in our hospital were selected, randomly divided into observation group and control group,60 cases in each group, the observation group treated with Tanreqing injection and oxygen, anti-inflammatory, expectorant therapy,the control group treated only with oxygen, anti-inflammatory, expectorant therapy, the clinical symptoms and therapeutic effects were compared between the two groups.Results After seven days of treatment, PaO2 , SaO2 and pH of the observation group were higher than those of the control group (P<0.05), the levels of PaCO2, CRP, IL-6, IL-13, LTB4 and WBC were lower than those in the control group (P<0.05); After 14 days of treatment, CRP, IL-6, IL-13 and LTB4 in the observation group were lower than those in the control group (P<0.05); The total effective rate was 75.00%in the observation group and 58.33%in the control group after 14 days of treatment.There was significant difference between the two groups (P<0.05).Conclusion Tanreqing injection combined with western medicine antibiotic treatment of elderly patients with pulmonary infection was significant,can significantly improve the clinical symptoms of patients and improve clinical efficacy .

Objective To discriminate the species and individual identification with mitochondrial DNA (mtD-NA) sequencing in forensic medicine practice. Methods The multiplex PCR of mtDNA loop - D high - variation region and cytochrome- b region were investigated. The PCR products were detected with silver- stain method,followed by analysis of the PCR products with fluorescence sequence technique. Results The presence of two bands (358bp,279bp ) indicated the samples were from human, while only one band of 358bp indicated nonhuman origin. The part of mitochondrial DNA loop - D high - variation region (15997 ~ 16236) from 131 unrelated individuals of Guangdong population were sequenced. In all of these samples there were 69 nucleotide variations and 67 haplo-types.There was 2.679 mutation sites on average per person. The polymorphism was 97.92% . Conclusion The methods described here are reliable and very useful in species and personal identification of degraded samples.

Objective:To study the diagnostic value about enhanced CT and gastroscopy for esophageal varices.Methods: Ninety eight patients with esophageal varices were selected as objects. All the patients underwent enhanced CT and gastroscopy, took detailed records of the test results, and analyzed CT scan at the portal vein and spleen vein values.Results: The results of enhanced CT detection and gastroscopy diagnosis were similar in liver cirrhosis combined with esophageal varices, liver cirrhosis combined with esophageal and gastric fundus varices, and liver cirrhosis complicated with gastric fundus varices. The diagnosis of enhanced CT is significant difference (F=33.06,F=20.22;P<0.05) in detection of portal vein value and spleen vein value for mild, moderate, and severe patients.Conclusion: The enhanced CT and gastroscopy for detecting of esophageal and gastric fundus has satisfactory value, and enhanced diagnostic CT for detecting severities of the esophageal and gastric fundus varices also has a important significance.

Objective To investigate the organic pollutants in the contaminated water irrigation area soil and to study the lipid peroxidation effect of the pollutants on mouse. Methods The organic pollutants of the polluted irrigation soil and control soil were extracted using the supersonic oscillator. The ingredients of the organic pollutants were measured by the GC-MS spectrum. The 40 sanitary Kunming mice were randomly divided into five groups: the dimethylsulfoxide control group,low dose group of control soil,high dose group of control soil,low dose group of polluted soil,high dose group of polluted soil. The administered doses of the low and high dose groups were 25.5 g dry soil/kg body weight per day by oral for two weeks. The activities of T-SOD,GSH-Px and the content of MDA in serum of mice were determined. Results Phenanthrene,yrene,diphenyl,fluorene,naphthalene,dimethyl-naphthalene,2,3,6-trimethyl-naphthalene,anthracene,dimethyl-anthracene,1,4-dimethyl-anthracene,fluoranthene,4-methyl-pyrene,dibenzothiophen,4-methyl-dibenzofuran,dibenzofuran,butylated-hydroxytoluene,dodecane,neopentyl-cyclohexane,trimethyl-heptane,1,2-dimethyl-cyclohexanimine,butethal were detected in the polluted irrigation soil and their levels were 14.3,2.1,1.3,1.1,0.8,1.3,0.4,2.9,7.2,2.3,9.1,0.2,0.4,1.4,1.7,0.37,0.9,0.11,0.02,0.02 and 0.13 mg/kg respectively in the polluted irrigation soil. Trimethyl-heptane,dimethyl-heptane and 1,2-dimethyl-cyclohexanimine were detected,and their levels were all 0.01 mg/kg in control soil. Compared with the solvent of dimethylsulfoxide group,the activities of T-SOD and GSH-Px of the high dose group of control soil and two dose groups of polluted soil all decreased,the differences were all significant(P0.05). Conclusion The soil has been contaminated with organic pollutants in the investigated area and the soil extract may produce a certain effect on lipid peroxidation in mice.

Objective To explore the anxious condition and the influencing factors of chronic hepatitis B patients, and to take appropriate care measures. Methods Self-designed questionnaire for general information of patients, a comprehensive Zung self-rating depression scale(SDS)and Zung self-rating anxiety scale(SAS)were used to investigate 100 patients with chronic hepatitis B patients(the study group), and the results were compared with 80 normal persons(the control group). Results The SDS and SAS score of the study group was significantly higher than the control group. The negative emotion factors of the study group included social context, family, education, self- respect. Conclusions Patients with chronic hepatitis B has obvious negative mood, and are influenced by many kinds of factors. Strengthening the comprehensive psychological intervention can improve the negative emotional state, and promote rehabilitation of patients.

ObjectiveTo investigate the related factors of the occurrence of nosocomial infection and nursing management in operating room for general surgery patients,and provide a scientific basis to reduce the incidence of nosocomial infection of the operating room. Methods304 patients with nosocomial infection from August 2009 to August 2010 in our hospital for surgery were set as the control group.151 cases from September 2010 to October 2011 were named as the intervention group.All cases were analyzed retrospectively.The nursing factors which might affect nosocomial infection in operation room were analyzed by univariate and multivariate logistic regression analysis. ResultsBy univariate analysis,eight operation room nursing factors related to nosocomial infection were screened out:the type of incision,operation time,the following surgery or not,into ICU time,preoperative use of antibiotics,visitors of operations or not,invasive operation.5 highly correlated factors were screened out by multi-factor logistic regression analysis:admission to the ICU time,incision type,operation time,preoperative use of antibiotics,then following surgery.The infection rate and prolapse rate of the control group were significantly higher than those of the intervention group. ConclusionsIn nursing work of the operation room,we can take a series of nursing management interventions to reduce the incidence of nosocomial infection of general surgery.

Objective To investigate the relationship between the serum levels of vascular endothelial growth factor(VEGF) and severity and prognosis by evaluating its changes in severe sepsis patients. Method Us-ing control study design, a total of 29 severe sepsis patients who admired in ICU department of Second Hospital of Tianjin Medical University from July 2006 to November 2007 were enrolled. The patients were divided into survival group (n=16) and death group (n=13) according to the clinical outcomes at 28 days after onset.A total of 31 healthy persons were enrolled into the control group. Clinical and laboratory data including blood routine test,blood-gas analysis,blood chemistry,C-reactive protein,lactic acid were collected on the first,third and 7th day after on-set,respectively. APACHE Ⅱ score were calculated. VEGF levels were determined using ELISA method. Quantita-tive data were analyzed by Ftest. Results The VEGFlevels ofthe control groupwere (78.77±8.15) pg/mL, the VEGF levels of the survival group on the first,third and 7th day were (210.47±59.40) pg/mL, (161.79 ±32.58) pg/mL and (85.33±12.13) pg/mL, respectively. The peak value of VEGF levels appeared on the first day, Then,it decreased with the progression of the disease. The VEGF levels did not differ significantly between the control group and survival group on the 7th day (P>0.05). The VEGF levels in the death group on the first, third and 7th day were (324.12±44.35) pg/mL,(185.40±30.92) pg/mLand (273.32±55.23) pg/mL, respectively. The peak value of VEGF levels in the death group also appeared on the first day, but it did not de-crease significandy on the 7 th day as compared that on theist day. The value of VEGF levels on the 7 th day were significantly higher than that of the control group (P<0.01 ). The levels of VEGF were positive correlated with APACHEⅡ score(rs = 0.510,P<0.01), both VEGF levels and APACHEⅡ score were risk factors for the clinical outcomes of these patients. Conclusions The serum levels of VEGF are elevated at early stage in severe sepsis patients. The VEGF levels, which might be a potential prognositic factor for sepsis patients are significantly correlated with APACHE Ⅱ score.

objective To detect MRSE and resistance in staphylococcus epidermidis isolates. Methods MRSE isolates were detected by cefoxitin disk test and susceptibilities were tested by agar dilution method. Results 79.7% of 128 isolates were MRSE strains with resistance to most antimicrobial agents, but those isolates were still susceptible to vancomycin and teicoplanin. Resistant rate in MRSE was higher than that in MSSE to most antimicrobial agents except penicillins, glycopeptides and macrolides. Conclusion MRSE with multi-resistance ale highly prevalent in staphylococcus epidermidis isolates and no isolate is resistant to glycopeptides.