Objective To study the efficacy of insulin pump therapy for type 2 diabetes and its impact on quality of life.Methods 60 patients with type 2 diabetes were selected and divided into A group and B group according to a random number table,30 cases in each group.The patients of B group were given conventional therapy,and which of A group was treated with insulin pump.The fasting plasma glucose,0.5h fasting blood glucose and 2h postprandial blood glucose were measured and compared before and after treatment,the quality of life was evaluated by quality of life scale(QOL),the QOL score before and after treatment between two groups was compared.Results The fasting plasma glucose,0.5h fasting blood glucose and 2h postprandial blood glucose of A group after treatment were (5.69 ±0.19)mmol/L,(5.28 ± 1.03) mmol/L and (7.31 ±2.14) mmol/L,respectively,which were significantly lower than (6.72 ± 2.04) mmol/L,(6.39 ± 1.69) mmol/L and (9.72 ± 1.57) mmol/L of B group (t =9.798,10.023,9.971,P =0.035,0.027,0.032) ; The QOL score of the group A was (78.4 ± 0.8) points,which was significantly better than that before treatment (t =9.064,P =0.023),and better than that of group B (t =10.853,P =0.019).Conclusion Insulin pump has better clinical efficacy in the treatment of type 2 diabetes compared with conventional therapy,it can effectively control blood sugar and improve the quality of life of patients.

Objective To analyses the effect of Buzhong Yiqi Decoction on the expression of Caspase-3 in human lung adenocarcinoma cells (A 549) tumor-bearing mice’spleen. Method 24 BALB/c mice were randomly divided into the control group(given saline),low-dose and high-dose Buzhong Yiqi Decoction groups,cisplatin(DDP)group;Buzhong Yiqi Decoction was administered twice daily for 10 days consecutively and DDP was administered once daily for 3 days consecutively, after 15 days was the spleens of all tumor-bearing mice were obtained.The expression of Caspase-3 protein and Caspase-3 mRNA in every group were detected by immunohistochemical method seperately and polymerase chain reaction method seperately. Results Compared with control group,the result of immunohistochemistry showed that the expression of the Caspase-3 protein in Buzhong Yiqi Decoction low-dose group and high-dose group were both ssignificantly increased (P<0.05). The differences hetween DDP group and control group was not significant, but the differences between DDP sroup and Buzhong Yigi Decoction Low-dose sroup and hishdoe group were significant (P<0.05, P<0.01).The result of RFPCR was accordance with the result of immunohisto chemistry. Conclusion Buzhong Yiqi Decoction can increase the expression of Caspase-3 protein in A549 ctumor-bearing mice’spleen,and promote the Caspase-3’s inducing effect on tumor cell apoptosis.

Objective To analyze the onset, clinical manifestation, causation, complications of pediatric uveitis. MethodsOne hundred and two patients with uveitis under 16 year-old were retrospectively studied. They visited the clinic in Peking University First Hospital from November 1979 to December 2008. Their age ranged from 2.5 to 16 years old, with a mcan of 11.9 years. Routine exam was carried out, including visual acuity, slit lamp, fundus, and laboratory workup. The diagnosis and classification were made by the anatomic location according to the standard of The International Uveitis Working Group. The data of disease history, age of onset, manifestation, recurrence, causation, systemic diseases, complications, and lab examination were analyzed. Results A total of 102 patients (170 eyes)with pediatric uveitis were included in this study, 68 patients (66.6%) were bilateral cases. Anterior uveitis represented in 38 patients (37.3% ), intermediate uveitis in 19 (18.6%), posterior uveitis in 10 (9.8%),and panuveitis in 35 (34.3%). The disease duration was from five days to 2.4 years, with a mean of 3.6 months. The follow-up time was two weeks to more than ten years. The first three causes of pediatric uveitis were juvenile idiopathic arthritis, Vogt-Koyanagi Harada disease, and Behcet's disease. 36 patients were found with complications, and among them 19 had complicated cataract, seven had secondary glaucoma, five had corneal band dystrophy, 12 had iris synechia (both anterior and posterior), one had retinal detachment, two had eye atrophy, and one patient with juvenile idiopathic arthritis had bilateral femoral head necrosis because of the use of steroid and hip joint was replaced. There were ten children suffering more than two complications. Conclusions Pediatric uveitis is a possible blindness disease with variety of etiology and manifestations, and tends to cause complications. Early and special attention must be taken to avoid serious consequences.

Objective To investigate the relationship between concurrent mental disorders and original respiratory diseases.Methods 35 cases diagnosed as severe respiratory diseases accompanied by various mental disorders from July 2005 to December 2008 were analyzed with the clinical presentation,examination,diagnosis and treatment.Results After the treatments of the original respiratory diseases and the necessary psychiatric drugs,the original respiratory diseases relieved and the symptoms of mental disorders disappeared.Conclusion Much more attention should be paid to concurrent mental disorders of respiratory diseases,and the timely treatments of symptoms of mental disorders will be of benefit to the therapy of original diseases.

OBJECTIVE To approach pathogens and precaution strategy of nosocomial pneumonia in patients with myasthenia gravis.METHODS Forty cases of myasthenia gravis from Jan 2003 to Dec 2006 in a certain hospital were involved in the retrospective review.Patients with myasthenia gravis and nosocomial pneumonia were selected out in accordance with diagnostic criteria of nosocomial pneumonia.Risk factors and pathogens of infection were analyzed.Precaution strategy was formulated.RESULTS There were 26 patients with nosocomial pneumonia among 40 cases of myasthenia gravis.Pathogens isolated from sputum culture were all drug resistant strains.Gram-negative bacteria such as Klebsiella pneumoniae,Pseudomonas aeruginosa,Escherichia coli,etc were the most,accounted for 51.85%(84 strains).The next was Gram-positive bacteria,accounted for 33.33%(54 strains).Fungi ranked the third,accounted for 14.81%(24 strains).CONCLUSIONS Precaution and cure measurement should be adopted to prevent and control the occurrence of nosocomial pneumonia in patients with myasthenia gravis.

Objective To investigate the present mental disability situation in Hubei provinee and the reha- bilitation strategies in use. Methods Provincial data from the Second China Sampling Survey of Disability was ana- lyzed. The 31 districts sampled in the survey were divided into a city group and a countryside group. The incidence of mental disability reported in the two groups was compared. Results Reported mental disability was found to be sig- nifieantly more prevalent in the countryside than in the city. However, the proportion of severe mental disability was significantly lower. Conclusion The need for rehabilitation of mental disability is huge in Hubei province. Society and the government should pay more attention to the rehabilitation of mental disability.

This study was aimed to explore the methodology regarding culture, proliferation and purification of adipose-derived stromal cells (ADSCs), and to study their biological characteristics. ADSCs were obtained using type I collagenase digestion method. Cell growth was observed, and cell viability were detected under different digestion period by MTT. The ADSCs were then identified and induced. The results showed that adherent cells digested by type I collagenase for 60 min had a strong proliferation capability. After the induction of different inducers these adherent cells could differentiate into nerve cells and fat cells. The best digestion period was proved to be of 60 minutes in the experiment. The results indicate that stem cells with multilineage differentiation ability could be separated from adipose tissue, namely ADSCs.
Adipocytes ; cytology ; Adipose Tissue ; cytology ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Neurons ; cytology ; Primary Cell Culture ; Stem Cells ; cytology ; Stromal Cells ; cytology

Objective To investigate the effect of treatment with transplanted bone marrow mononuclear cells under different transplant paths on neovascularization in ischemic cerebral infarction rats. Methods The MCAO models were established by intraluminal vascular suture method. All the successful models were randomly divided into control group, intravenous transplantation group and subarachnoid transplantation group according to the random number table. The Extraction,isolation and culture of bone marrow mononuclear cells were extracted,i?solated and cultured. The models in the control group were injected 200μl PBS through the femoral vein. The mod?els in the intravenous transplantation group were injected 200μl containing 2×107 bone marrow mononuclear cells through the femoral vein. The models in the subarachnoid transplantation group were injected 200μl containing 2× 107 bone marrow mononuclear cells through the subarachnoid. The VEGF levels in cerebrospinal fluid of rats were detected using quantitative ELISA method. The vascular regenerations in the infarct area were observed by using quantitative immunofluorescence. Results After transplantation of bone marrow mononuclear cells 7 d,14 d and 28 d,the VEGF levels of rat cerebrospinal fluid in subarachnoid transplantation group were (207.4±8.9)pg/ml, (171.2±10.3)pg/ml and (143.8±13.8)pg/ml,respectively,which were all higher than the VEGF levels in intra?venous transplantation group,the differences were all statistically significant (P<0.05). After transplantation 14 d and 28 d,the BrdU?positive cells in subarachnoid transplantation group were ( 2043. 8 ± 514. 2) and ( 1834. 8 ± 307.4) ,respectively,which were higher than the intravenous transplantation group,the differences were all statisti?cally significant (P<0.05). The microvessel counts in subarachnoid transplantation group were (384.6±45.1) and (514.8±51.3),respectively,which were higher than that in the intravenous transplantation group,and the differ?ences were all statistically significant (P<0.05) . Conclusions Bone marrow mononuclear cells transplantation can increase VEGF secretion,and promote angiogenesis infarct. The effect of subarachnoid transplantation is better.

0.05). Conclusion There are MA and mutation of p53 gene in T-cell lymphoma though no significant correlation between them. But, MA positive cases might experience high mutation of p53 gene in T-cell lymphoma.

Objective To observe the effect of Hydroxycamptothecin (HCPT) on proliferation of human lung cancer cell line A549 in vitro. Method Using cell calture and MTT assay to observe the effect of HCPT on proliferation of human lung cancer cell line A549. Result Lower concentration of HCPT had no evident inhibitory effect on the human lung cancer cell line A549 after 24 h. The effect was evident when the concentration of HCPT was up to 50 ?g/mL, and the inhibitory rate was 44.17%. The inhibitory rate was 50.28% when the concentration of HCPT was up to 100 ?g/mL. The inhibitory effect of HCPT became more significant with the stimulation of the time, and the inhibitory rate of 100 ?g/mL concentration of HCPT was 70.98% after 48 h. Conclusions HCPT can inhibit the proliferation of human lung cancer cell A549 in vitro. The effect is dose and time dependent.