The phenotypes of human fetal thymocytes has been analysed with fflowcytometry.The findings suggest that the composition of thymocyte subsets shows no obvious changes from the 17th week of gestation to birth.In the pieriod of human fetus,features of thymocyte phenotypes are showed:1.Expression density of CD3/TCR?? shows a continuous distribution from low to high.According to the density of D3/TCR??,tyhmocytes can be divided into three subsets,that is,L—CD3/TCR??(low—density),M—CD3/TCR?? medium—density)and H—CD3/TCR??(high— density).The proportion of three subsets is different in different fetuses.2.Single positive thymocytes express CD1 antigens.H—CD3/TCR?? thymocytes also express CD1 antigens.But,Part of those thymocytes express low—density of CD1 antigens,other part of the hymocytes express high—density of CD1 antigens.3.HLA class Ⅰ antigen density varies with thymocytes.Most thymocytes express low density of HLA class Ⅰ antitgens,minor thymocytes express high density of HLA class Ⅰantigens.HLA class Ⅰ antigen density correlates with maturity of thymocytes.4.Thymocytes can be divided into two subsets:Thy-L and Thy-H on the basis of thymocyte special density by means of density gradient centrifugation.Thy-L subset includes more mature and early thymocytes than Thy-H subset,while thy-H subset includes much more common thymocytes than Thy-L subset.

The postoperative neurological complications is associated with intraoperative cerebral emboli, which results from extracorporeal circulation and operation. It can effectively reduce the incidence of neurological complications with ultrasonic radiation. In fluids, a particle will change it's motion trail when it is acted by the radiation force generated by the ultrasound. This article mainly discuss how to shielding emboli with ultrasound. The equipment can transmit phased ultrasonic signals, which is designed on a FPGA development board. The board can generate a square wave, which is converted into a sine wave through a power amplifier. In addition, the control software has been developed on Qt development environment. The result indicates it's feasible to shielding emboli with ultrasonic radiation force. This article builds a strong foundation for the future research.
Humans ; Intracranial Embolism ; diagnostic imaging ; prevention & control ; Intraoperative Complications ; prevention & control ; Postoperative Complications ; prevention & control ; Ultrasonics ; instrumentation ; Ultrasonography

BACKGROUND:Three-dimensional (3D) nanofiber scaffolds are aimed to mimic the physical and chemical signals of stem celsin vivo. 3D nanofiber scaffolds, which are capable to maintain the activity of stem cels, are promising in tissue regeneration and stem celltherapy. OBJECTIVE:To review the synthesis methods for 3D nanofiber scaffolds, and the interactions of stem cels and 3D scaffolds as wel as the current progress of 3D nanofiber scaffolds in tissue engineering. METHODS:Web of Science was searched with key words of “tissue engineering, nanofiber scaffold, stem cellfate” in English for articles relevant to stem celltissue engineering and 3D nanofiber scaffolds. RESULTS AND CONCLUSION:3D nanofiber scaffolds can mimic the physical architecture ofin vivo microenvironment due to its nano-scale topology. Chemical modification of scaffolds endows chemical cues to stem cels. Therefore, 3D nanofiber scaffold can be a promising delivery vehicle of stem cels in tissue engineering. Nanofiber scaffolds can be synthesized through self-assembly, sol-gel phase separation, and electric spinning. 3D nanofiber scaffolds have been shown to improve thein vitro proliferation of hematopoietic stem cels, embryonic stem cels, mesenchymal stem cels, and neural stem cels. 3D nanofiber scaffolds with specific topology/chemical properties can induce the differentiation of stem cels into bone, cartilage, nerve, or muscle. 3D nanofiber scaffold which provides a satisfactory microenvironment for stem cellenhance the performance of stem celltherapy.

BACKGROUND:Construction of tissue engineering adult cardiac myocytes has been a new research hotspot in cardiovascular fields.
OBJECTIVE:To explore a simple, fast method for the separation of adult rat cardiomyocytes, and preliminarily explore the construction methods of tissue engineering adult cardiac myocytes.
METHODS:Segmented enzymatic digestion method was used to isolate cardiac myocytes from adult rats. Subsequently, cardiac myocytes were transfected with adenovirus and liposome-mediated red fluorescent protein gene. Construction efficiency of tissue engineering cells was qualified using inverted fluorescence microscopy and flow cytometry. Final y, cardiac myocytes were transfected with adenovirus-mediated hypoxia-inducible factor-1a, and the expression of hypoxia-inducible factor-1a was examined by western blot analysis.
RESULTS AND CONCLUSION:A lot of cardiac myocytes were col ected using the segmented enzyme digestion method. Flow cytometric analysis showed that the survival of adult cardiomyocytes was (87.03±0.70)%. Compared with liposomal transfection (transfection efficiency was 0), adenovirus infection efficiency was (70.31± 1.39)%, and the cells expressed red fluorescence under fluorescence microscope. After 4 days of adenovirus infection, transfected cells expressed hypoxia-inducible factor-1a protein. These results showed that segmented enzyme digestion is a fast way to isolate adult cardiac myocytes, and recombinant adenovirus vector is a good vector to transfect cardiac myocytes.

Objective To study the role of corn oil in the change of serum glucose, serum lipids and insulin in type 2 diabetes rats with insulin resistance, and attempt to find out its mechanism. Method High fat and sugar diet plus low-dose streptozotocin (STZ) was given to establish type 2 diabetes rat with insulin resistance model, the rats of which were randomly divided into normal group, model group, corn oil (0.8 mg/kg) group, rosiglitazone group. After Ig 6 weeks, fasting blood glucose (FBG), total cholesterol(TC), triglyceride(TG), low density lipoprotein(LDL), high density lipoprotein (HDL), fasting insulin (FINS), and insulin resistance index (HOMA-IR) were detected. Results Corn oil can elevate the level of HDL in diabetic rat, lower the levels of FBG, TC, TG, LDL, FINS and HOMA-IR. Conclusion Corn oil can improve insulin resistance, and decrease the serum glucose and lipid of type 2 diabetic rats.

Objective To know the effect of ultraviolet A on energy metabolism in the cells in culture by using the primary cultured postnatal rat cortex neurons for in which there are many mitochondria. Methods The primary cultured postnatal SD rat cortex neurons in 60 mm dishes were exposed to UVA in a chamber in doses of 0, 3, 6 and 9 J/cm2 respectively. The exposure was followed by 4 hours culture and then the activity of cytochrome oxidase (COX) in the single neuron was determined by cytochemisty, the quantitative analysis was done with Image-Pro plus 4.0. Results In every exposure groups, after UVA exposure followed by 4 hours culture, no significant changed vital signs were found under the microscope, the activity of COX in the neurons significantly decreased compared with the control and a dose-response relationship was presented. Conclusion Low level UVA exposure may cause obvious energy metabolism decrease in the cells in culture before the significant changed vital signs are seen.

OBJECTIVE:To evaluate the quality of Artemisia annua L. samples planted in different site condition in Chongqing Wulong county and to provide a fast and convenient assaying of artemisinin in Artemisia annua L. samples in the industrial development of this Chinese herbal medicine. METHODS: The Artemisia annua L. planted in different site condition in Chongqing Wulong county were sampled during harvesting time, which were subjected to many processing steps such as drying by baking, crushing, microwave extraction and color reaction etc. Then the absorbance of the test liquid was detected by UV-spectrophotometry at a wavelength of 290 nm. Finally Artemisinin content was computed based on the regression curve of standard solution and the quality of the Artemisia annua L. samples was evaluated. RESULTS: The liner concentration range of Artemisinin was 8～40 mg?mL-1 (r=0.999 8) with an average recovery rate of 99.98% (RSD=1.48%, n=9).CONCLUSION: The results showed that site condition did have effect on the quality of Artemisia annua L. to some degree. UV-Vis method is simple, rapid and reproducible, and suitable for the quality control of Artemisia annua L. samples.

The principles, traits, and proceedings of the data-driven methods including principal component analysis, independent component analysis and canonical analysis are summarized. And the aspects of the methods adapted to functional magnetic resonance images(fMRI) data are emphasized.

Objective To establish a reversed HPLC method to determine the contents of gatifloxacin and tinidazole simultaneously. Methods ODS C_ 18 column (4.6mm?250mm,5?m) was used as an analytical column. The mobile phase consisted of 15% acetonitrile, 25% of methanol and 60% of 0.05mol/L KH_2PO_4 buffer,which contained 0.5% triethylamine and was adjusted to pH 3.5 by phosphoric acid. The flow rate was 1ml/min. The detection wavelength was 318nm. The column temperature was 30℃. 20?l of diluted sample solution was injected to the sampler. Results The calibration curve of gatifloxacin was linear in the range of 2~20?g /ml (r=0.999 6) ,and that of tinidazole was 2~20 ?g /ml (r=0.999 9). The recovery rates of them were 100.1% and 100.4%,respectively. RSDs of both drugs were less than 1.1 %. Conclusions The method is simple,accurate and sensitive and can be used to determine the two components simultaneously.

Objective to compare the calculated effective radiation dose variations in cardiac interventional procedures of coronary angiography(CAG)and percutaneous transluminal coronary angioplasty (PCI)by using the different tissue weighting factors recommended by ICRP Publication 60 with that recommended by ICRP Publication 103.Methods Measurements of organ doses were carried out with an anthropomorphic phantom equipped with thermoluminecent dosimetry.The effective radiation doses were separately calculated using the tissue weighting factors provided by ICRP 60 and provided by ICRP 103,and the results obtained from ICRP 60 were compared with that obtained from ICRP 103.Conversion factors of effective radiation dose to dose area product(DAP)were also calculated according to different publication. Results The variations in the effective dose were 6.88%for CAG and 8.46%for PCI.The effective dose variations in CAG and PCI were 7.25%and 8.76%for female and were 6.51%and 8.17%for male, respectively.The effective conversion factors of DAP were 0.10～0.11 and 0.13～0.14 for CAG and PCI, respectively,according to ICRP 60 and 103.Conclusion The variation of conversion factors of effective dose indicates that in making estimation of effective radiation dose by using the widely accepted conversion factors,the adjustment of organ weighting factors recommended by ICRP 103 should be considered.