Objective To estimate the effect of the NO on the medial amygdaloid nucleus(Me), we studied the origins of the NOS positive terminals in the Me. Methods Noergic afferent projection to Me was identified by a combined NADPH-d histochemical staning and retrograde CTb immunocytochemical method after microinjecting CTb into Me. Results The double labeled of neurons (NOS and CTb) were located in dorsal raphe nucleus, locus ceruleus, basolateral amygdaloid nucleus, parabrachial nucleus, ventrolateral part of periaqueductal gray.Conclution The NADPH-d positive terminals in the Me originates from the aforementioned nucleus, and may relate to the function of the Me.

Objective　To investigate the epidemiological characteristics of pulmonary tuberculosis among students in Fuzhou City in 2022 and the screening of their close contacts, so as to provide scientific basis for further prevention and control of tuberculosis in schools. Methods　A descriptive method was used to investigate the campus tuberculosis epidemic situation in 87 colleges and universities in Fuzhou, and 96 cases of confirmed student patients were investigated individually, and 7 488 close contacts were screened according to the requirements of the Guidelines for tuberculosis Prevention and Control in Chinese Schools, and the results were analyzed. Results　In 2022, a total of 96 students with pulmonary tuberculosis were found in Fuzhou City, with a male to female ratio of 1.04∶1 and an average age of 17.92 ± 4.16 years. The incidence rate of active pulmonary tuberculosis and the incidence rate of bacterial positive pulmonary tuberculosis among university and college students were higher than those among other types of schools, separately 10.56/100 000 and 5.18/100 000, with significant differences (χ2=75.22, P<0.05; χ2=38.69, P<0.05). The proportion of student patients was the highest in the first quarter (34.38%, 33/96). There were 44 cases of delayed visits, with a delay rate of 45.83% (44/96). The diagnosis was delayed in 15 cases, with a delay rate of 15.63% (15/96). Screening of 7 488 close contacts, of which 5 339 were screened with chest radiography and 5 active cases were found. A total of 7 124 patients were screened by the tuberculin skin test (TST test), the positive rates of TST test results for close contacts of pathogenic and no pathogenic patients were 25.97% (1 135/4 371) and 21.10% (581/2 753), respectively. The strong positive rates were 6.15% (269/4 371) and 3.01% (83/2 753), respectively, with statistically significant differences (χ2=21.84, P<0.05; χ2=35.44, P<0.05). Conclusion　In 2022, the incidence of tuberculosis in schools in Fuzhou City was dominated by students from universities and colleges, with the highest incidence rate in the first quarter. The delay in seeing a doctor and diagnosis of student patients was serious. Timely and effective screening of close contacts (especially the close contacts of patients with bacterial positive pulmonary tuberculosis) can reduce the spread of tuberculosis in schools.

Objective To evaluate the application effect of UK manual moving & handling skills in the training of preventing occupational lower back pain among nursing professionals,and explore effective ways of training on preventing occupational lower back pain to nursing professionals.Methods 86 nursing students,94 nurses with 12 months' work experience,and 88 nurses with work experience over 5 years were randomized selected,and they each were divided into the experimental group and the control group.For 86 nursing students,43 students in each group; For 94 nurses with 12 months' work experience,47 nurses in each group; For 88 nurses with work experience over 5 years,44 nurses in each group.A routine taught training was given to both groups,while the experimental group received an extra 25 academic hours' structured OLBP prevention training.Comprehensive proposition was used to assess theoretic learning outcome of all participants,and practical skills of all participants were tested through institutional cases.Ressults There was significant statistical difference between scores of participants from two groups,and the scores of the experimental group in nurses with 12 months' work experience were different from the experimental groups of nursing students and nurses with work experience over 5 years.Conclusions Structured OLBP prevention training should lead occupational health prevention and health promotion concepts,apply numerous teaching approaches,emphasize the content of maintaining physical and psychiatric health and occupational protection management,set new-enrolled nurses as the main focused group of training.

Objective To investigate the influence of ursolic acid on vascular endothelial growth factor ( VEGF) , cycloxygen-ase-2 (COX-2), and matrix metalloproteinases-2 (MMP-2) expressed in the mouse retinal ischemic model , and to explore the mecha-nisms of anti-angiogenesis.Methods Sixty 7-day clean-class C57BL/6J mice were divided randomly into 6 groups [ n =10 mice (20 eyes) per group]:blank control, model control (PBS), positive control (triamcinolone), and ursolic acid (UA) intervention (low-dose, medium-dose, and high-dose).Mice in the blank control group were raised in air , and mice in other groups in(75%±2%)O2 high-oxygen environment for 5 consecutive days .Mice in the model control group and breastfeeding mice were put back in air environ-ment (21%O2 ) on the 12th day after the new-born mice to induce the generation of retinal neovascularization .When models were suc-cessful, the drug treatments were applied immediately to the corresponding groups , with injection of 3μl of sterile PBS in model control group, 3 μl of 1.5, 3.00 and 6.0 μg UA in UA intervention group, and 3 μl of triamcinolone (1 ml∶40 mg) in positive control group, respectively.All mice were killed after overdose anesthesia on the 17th day.Their eyeballs were made into samples and retinal tissue pathological sections with H-E dying method.The positive expressions of VEGF , COX-2, and MMP-2 were detected with immu-nohistochemical method .The fresh retinal tissue homogenate was prepared to detect the protein expressions of VEGF , COX-2, and MMP-2 in retinal tissue with western blot method ,and mRNA expressions of VEGF , COX-2, and MMP-2 were detected with real-time fluorescent quantitative polymerase chain reaction ( RT-PCR) .Results According to protein and mRNA expressions of VEGF , COX-2,and MMP-2 in retinal tissue among six groups , protein expressions of VEGF , COX-2, and MMP-2 in model group were significantly higher than those in blank group ( P <0.05 ) .Each protein expression in the high UA intervention group was significantly lower than that in the model group ( P <0.05 ) .Each protein expression in the high UA intervention group was not significantly different from that in the positive group ( P >0.05 ) .Each protein expression in the high UA intervention group was significantly lower than that in the low UA intervention group( P <0.05).Conclusions UA inhibited expressions of VEGF, COX-2, and MMP-2 in retinal ischemia model .UA also played an inhibitory role in the formation of neovascularization , and this role was positively correlated with UA dose .

The basement membrane (BM) is crucial in regulating the physical and biological activities of esophageal epithelial cells which attach to the underlying BM. In order to simulate the natural construction of BM, we prepared the fibrous scaffolds using biodegradable polylactide (PLA) and silk fibroin (SF) as the materials via electrospinning technology. BM's proteins containing collagen (IV), laminin, entactin and proteoglycan were extracted from porcine esophagus and coated on the eletrospun fibers. Morphology, mechanical strength, biodegradability and cytocompatibility of the coated and uncoated scaffolds were tested and evaluated using scanning electron micrography, mechanical test system, immunofluorescence assay and western blotting with CK14 as the primary antibody. The fibrous scaffold PLA or PLA/SF, generated from the present protocol had good formation and mechanical and biodegradable properties. After coating with BM's proteins, the scaffold could enhance the growth and differentiation of esophageal epithelial cells, which would contribute to remodel and regenerate the tissue engineered epithelium and further contribute to engineer the whole esophagus in future.
Absorbable Implants ; Basement Membrane ; Biocompatible Materials ; chemistry ; Epithelium ; Esophagus ; physiology ; Fibroins ; chemistry ; Humans ; Nanostructures ; chemistry ; Polyesters ; chemistry ; Regeneration ; physiology ; Tissue Engineering ; methods ; Tissue Scaffolds ; chemistry

Objective To summarize the main points of nursing the patients during small incision minimally invasive cardiac surgery.Methods Two hundred and seventy-one patients underwent small incision minimally invasive cardiac surgery during April 2010 and December 2010.The cooperative nursing was performed.Results The patients went well with the operations,with the average extracorporeal circulation time of(117.3±47.1)min and aorta blocking time of(82.6±32.1) min.All of the patients were discharged,without postoperative complications.(6.8±2.6)d.Perioperative and after discharge with no deaths.Postoperative recovery and no postoperative complications,were recovered and discharged without any complications.The postoperative hospital length of stay was(6.8±2.6)d.Conclusions The minimally invasive cardiac surgery is effective and safe.The preoperative health education and intraoperative nursing cooperation are critical for the operative success.

Objective To investigate the clinical treatment efficacy of mutabilis coated nasal ointment to postoperative nasal septal reconstruction.Methods After surgical reconstruction,116 cases were divided into the treatment group and control group ac-cording to random method,58 cases in each group.The treatment group was given mutabilis coated nasal ointment combine with physiological seawater nasal care device;the control group was only given physiological seawater nasal care device in the same time. The effects were observed for 6 months.Results The total effective rate of treatment group was higher than the control group (98.28%),the two groups had significant difference(P <0.05).Postoperative follow-up,the bleeding rate in the treatment group have no significant difference compared with the control group(P >0.05);the incidence of infection and cavity stricture in the treat-ment group were significantly lower than the control group(P <0.05).Conclusion The application of mutabilis coated nasal oint-ment can promote the wound healing of nasal cavity,but also can prevent the operation adhesion,and shorten repair time,so as to improve the clinical effect of operation.

Objective: To investigate whether Vigconic VI-28 capsule, a formulated traditional Chinese medicine containing radix Ginseng, cornu cervi pantotrichum and semen cuscutae, can assist tumor chemotherapy in a mouse model. Methods: Female C57BL/6 mice were s. c. injected with viable Lewis lung cancer ( LLC) cells (106 cells/mouse) . The mice were then treated with cyclosposphamide (Cyp, 40 mg/kg bodyweight, once every other day). One group was intra-gastrically given 2% VI-28 (0.5 ml/mouse, every other day) during the course of the chemotherapy. By day 28, the mice were sacrificed and their thymic indices and tumor indices were calculated and compared. Splenocytes were collected for analysis of their immunological status. Histological study was carried to examine the solid tumors. Results: Fourteen days after the injection of LLC cells, solid tumors developed in most of the animals, reaching 1 ~ 1.8 cm diameters by 28 th day Compared with mice of the LCC + Cyp group, thymus glands from the LLC + Cyp + VI-28 group were significantly heavier. Splenocytes of the same group responded better to ConA stimulation in vitro. Histochemical examination of the tumor tissues revealed that tumors of the Cyp + VI-28 group were better differentiated (less aggressive) than that of the Cyp group. Conclusion: Vigconic VI-28 capsule can promote recovery of immune system in mice undergoing chemotherapy and help Cyp to control the growth of tumor cells in vivo.

Objective To explore the establishment of health promotion mode to the independent elderly group at elderly care institutes.Methods To establish operational-training community health promotion mode based on Maslow's hierarchy of needs and Pender's health promotion theory,the mode was implemented among 86 healthy elderly people,and the effectiveness of mode implementation was evaluated.Results Scores of quality of life and health promotion lifestyle after applying health promotion mode showed statistical differences compared to scores before the implementation,and there was improvement of scores of interpersonal relationship,health responsibility and self-realization after applying health promotion mode.Conclusions Operational-training health promotion mode could help in enhancing health status as well as health promotion behavior of the independent elderly group in elderly care institutes,and promote the utility to community health resources,it is worth to promoting the mode in community health promotion among the elderly people in health care institutes.

ObjectiveThe expression and significance of interleukin(IL)-17 in a murine model of experimental systemic sclerosis(SSc) was studied and its correlation with transforming growth factor-beta 1 (TGF-β1) was explored.Methods Thirty female BALB/c mice were randomly divided into 3 groups,including a control group, bleomycin(BLM) injection for 4 weeks group(model 1 group) and a termination injection of BLM 4 weeks group(model 2 group).The pathological changes of skin and lung were detected.The mRNA expressions of IL-17A,RORγt,TGF-β1 mRNA were evaluated by real-time PCR.Enzyme linked immunosorbent assay was used to measure the levels of IL-17 and TGF-β1 in the serum and bronchoalveolar lavage fluid(BALF).Comparisons among groups were performed by variance analysis.ResultsSkin and lung of the model groups showed evident inflammatory cell infiltration and increased deposition of collagen fibers.The score of dermal inflammation and lung fibrosis was significantly higher in the model 1 and model 2 groups (2.5±0.8,3.0±1.8), (2.4±0.8,3.1±1.2) as compared to that of the control group (0.9±0.7,0.9±1.0),(F=12.19,8.367,25.11,4.641; all P＜0.05).The amount of hydroxyproline was markedly increased in the model groups than in the control group.Compared with those of the control group,the mRNA levels of IL-17A,RORγt,TGF- 31 in the skin and lung of the model 1 group were higher.The levels of IL-17 in serum and BALF of the model 1 group was significantly increased and the levels of TGF- β1 were increased in BALF and decreased in the serum (all P＜0.05).The mRNA levels of IL-17A in skin and lung had a positive correlation with the mRNA levels of TGF- β1,score of dermal inflammation and lung fibrosis.The levels of IL-17 in serum had a positive correlation with hydroxyproline of the skin and lung.ConclusionIL-17 may participate in systemic immune-mediated inflammation and changes of skin and lung in SSc and when combined with TGF-β1 togetter will cause damage to skin and lung in SSc.