OBJECTIVE To study the diagnostic and therapeutic conditions in a general hospital,and try to find out the measures to decrease cross infection in hospital. METHODS From Dec 2003 to Nov 2004,2 037 cases of infectious diseases were diagnosed and treated in our hospital.All these cases were analyzed. RESULTS Among the 2037 cases,the most common diseases were viral hepatitis and pulmonary tuberculosis.And others were infectious diarrhea,gonorrhea,syphilis,typhoid,paratyphoid,epidemic parotitis,bacillary dysentery,epidemic encephalitis B,measles,tetanus of newborn and rabies. CONCLUSIONS To control cross infection in hospital effectively,we must pay more attention to standardized management of infections diseases,strengthen the isolation and protective conciousness of medical personnel for infectious diseases,and tighten up the management of sterilization and isolation.

Objective To discuss nursing of patients with early severe acute pancreatitis undergoing laparoscopic treatment and in comparison with open surgery. Methods Totally 56 patients with early se-vere acute pancreatitis were admitted from October 2002 to October 2007,among which 30 cases (group A) received laparoscopic.treatment and the other 26 cases (group B) was given open surgery.The average time of hospital stay,incidence of complication and re-operation were compared between the two groups. Re-sults The average time of hospital stay,incidence of complication and re-operation of group A were lower than those of group B (P<0.05). Conclusions Treatment of severe acute pancreatitis with laparoscopy proved to be a desirable method in that it performs good effect with little trauma and low fatality.Other basic treatment,regulation of water and electrolyte balance and acid-base balance,enforcement of nutritional sup-port,fasting and gastrointestinal decompression should not be ignored.

OBJECTIVEThis study investigates the immune status of T helper (Th) 17 cells in mice with periodontitis.

METHODSSeven-week-old C57BL/6 female mice were randomly divided into the control and periodontitis groups. Each group comprisedfour mice. Experimental periodontitis was induced in mice by oral infection with Porphyromonas gingivalis in the periodontitis group. Phosphate-buffered saline solution was used in the control group. All mice were sacrificed 4 weeks after the last P. gingivalis infection. CD4⁺retinoid-related orphan receptor (ROR) γτ⁺(Th17) cells were analyzed by flow cytometry. The protein expression of Th17 cell-related cytokine interleukin (IL)-17A was detected by enzyme-linked immunosorbentassay (ELISA).

RESULTSFlow cytometry showed that the percentage of CD4⁺RORγτ⁺(Thl7) cells in all CD4⁺ cells and the cell number in gingival tissues, cervical lymph nodes (CLNs), and the peripheral blood obviously increased (P < 0.01) in the periodontitis group. ELISA showed that compared with the control group, the periodontitis group exhibited an obvious increase in the protein expression of IL-17A (P < 0.05).

CONCLUSIONTh17 cell-mediated cell response is enhanced, and the gingival tissues, CLNs, and the peripheral blood are probably the main locations of Thl7 cell-mediated cell response during the development of periodontitis.

Alveolar Bone Loss ; Animals ; Cytokines ; Female ; Flow Cytometry ; Gingiva ; Interleukin-17 ; metabolism ; Mice ; Mice, Inbred C57BL ; Periodontitis ; metabolism ; Porphyromonas gingivalis ; Random Allocation ; T-Lymphocytes, Helper-Inducer

Objective To evaluate the effectiveness of allergen specific immunotherapy (SIT) in the treatment of allergic rhinitis. Methods A total of 132 patients with seasonal ragweed allergic rhinitis were enrolled in this study. The patients were randomly divided into two groups. SIT group, in which 68 patients were treated with ragweed SIT for six months before July for 3 years. ST (symptomatic therapy) group, in which 64 patients were treated with ST during seasonal symptomatic period. The healthy control group included 40 subjects. The levels of serum total IgE (tIgE), specific IgE (sIgE), ECP, IgG and IL-4, IL-5, IFN-? were measured, and eosinophil numbers were counted. The symptom score, lung-function test and skin prick test were performed before and after the treatment. Results The elevated levels of tIgE, ECP, IL-4, IL-5 in the sera and eosinophil counts in peripheral blood of the patients were significantly reduced, whereas the levels of IgG and IFN-? were increased following SIT. The levels of serum sIgE were not changed in both SIT and ST groups. 30.88% of the patients were clinically cured, the effective rate was 41.18%, the improvement rate was 20.59% after SIT, and only in 7.39% of patients there was no clinical improvement. In 16.18% of patients there were mild side effects to SIT. In the ST group, there was on change in all the laboratory results and clinical symptoms, and 25% of the patients developed asthma. Conclusion SIT was able to improve the symptoms and laboratory results of the patients with allergic rhinitis. The results indicated that SIT may be an effective and safe therapy for patients with allergic rhinitis.

Objective:To probe the application and the effect of integration medical health education mode in the care-givers of children with capillary bronchitis. Method:Total 120 cases of capillary bronchitis caregivers were divided into control group (56 cases) and observation group (64 cases) and control group with traditional mode of health education, observing group adopts the integration medical health education mode;Compared two groups of children with hospitaliza-tion days, take care of health education knowledge of cooperation degree, satisfaction and health care work. Results:After implementation the integration medical health education mode, children hospitalization days declined. Caregivers' health education knowledge and satisfaction were obviously improved. The medical work cooperation degree is higher than the control group. Conclusions:The implementation of the new mode is helpful to improve work efficiency, reduce the finan-cial burden on families, and meet the demand of caregivers, health education and the right to know and sublimation of medical humanism philosophy, promoting the comprehensive qualities of nurses, improve patient satisfaction, harmonious relationship between hospital and patients, improve high quality nursing care for patients.

Objective To evaluate the efficacy and safety of specific immunotherapy (SIT) to allergic rhinitis in children.Methods Sixty-four patients with mite allergy allergic rhinitis in children,were divided into two groups by random digits table:treatment group and control group,each group with 32 cases.Treatment group was given SIT with standardized allergen vaccine for 3 years on the basis of symptomatic therapy,control group only received symptomatic therapy.Observation indexs included rhinitis symptoms score,drug score,skin index (SI),serum specificity IgE (sIgE),peripheral eosinophil (Eos) counting,development of asthma and the new sensitization.Results The Eos counting,SI after treatment 3 years in treatment group were significantly better than those before treatment and those in control group after treatment 3 years[(0.14 ± 0.12) × 109/L vs.(0.74 ± 0.18) × 109/L,(0.78 ± 0.36) × 109/L and 1.03 ± 0.13 vs.1.51 ± 0.32,1.51 ± 0.37] (P ＜ 0.01).There was no statistically significant difference in sIgE between two groups (P ＞0.05).The rhinitis symptoms score,drug score in two groups after treatment 1,2,3 years were significantly better than those before treatment (P ＜ 0.01).The rhinitis symptoms score,drug score in treatment group after treatment 1,2,3 years were significantly better than those in control group(P ＜ 0.01).The rate of new sensitization in treatment group was significantly lower than that in control group [3.1％ (1/32)vs.34.4％ (11/32),P ＜ 0.01].Conclusion Keeping long-term SIT is effective and safe for children's allergic rhinitis induced by mite,it also prevents new allergen appeared and allergic rhinitis development for asthma.

Objective To evaluate the efficacy of endoscopic techniques for corrections of facial contour deformities.MethodsA total of 58 patients(48 women and 10 men)with square face(38),prominent zygoma(12),or crooked noses(8)were treated by endoscopic-assisted technique in our hospital.Results The operation time ranged from 40 to 86 min(mean,65 min),and intraoperative blood loss was 60-120 ml(mean,78 ml).The patients were discharged from the hospital in 3 to 7 days(mean 4)after the surgery,and was followed up for 3 to 24 months.During the period,no one had unexpected fracture,injuries to the mental and facial nerves and vessels,or infection.All the patients were satisfied with the surgical outcomes.ConclusionsEndoscopic-assisted correction of facial contour deformities is feasible,safe,and effective.

Objective:To investigate whether lanthanum(La 3+)can enter into ossification induced bone marrow stromal cells(BMSCs)cultured in vitro and its influences on the ultrastructure of BMSCs.Methods:Dog BMSCs were ossification induced and the cells of the third passage were cultuerd with the presence of lanthanum chloride(LaCl_3)at 5.564?102,5.564 and 5.564?10-2 ?g/ml respectively for 5 days,the same passage cells without LaCl_3 treatment were used as the controls.Transmission electron microscope(TEM),electron microprobe with wave spectrum(EMWS)and high resolution TEM with energy spectrum(HRTEM)were used to observe the change of BMSCs ultrastructure and the distribution of lanthanum ions in the cells.Results:Electron-dense granules in lysosome were observed in BMSCs treated with LaCl_3 but not in control BMSCs under TEM.Slight denaturation of some organelles was found in both LaCl_3 treated and control cells.EMWS examination showed that there was La element in BMSCs treated with 5.564?102 ?g/ml LaCl_3.La element was not found in all the treated cells under HRTEM.Conclusion:La 3+ at a certain concentration might enter into the ossification induced BMSCs cultured in vitro.

Objective: To investigate the relationship between the ac tive granulae in mast cells and infant hemangiomas of parotid gland. Methods: Surgical samples of parotid gland hemangioma were obtained from 40 infants under the age of 6 months and those of normal parotid gland tis sue from 5.The samples were investigated by safranin staining,alcian blue and th ionine staining, and image analysis technique. Results: Th e percentage of thionine positive cells vs tissue area in hemangioma and normal parotid gland tissue was 1.132?0.195 and 0.335?0.122 ( P

Objective:To investigate regulatory T(Treg)cell-mediated immune response in mice with periodontitis.Methods:8 sev-en-week old C57BL/6 female mice were randomly divided into 2 groups(n =4).Periodontitis was induced in mice by oral infection with Porphyromonas gingivalis(P.gingivalis).Carboxymethyl cellulose(CMC)was used for the sham control mice.All mice were sacrificed 4 weeks after the last P.gingivalis infection.CD4 +forkhead box P3 (Foxp3)+ cells were analyzed by flow cytometry.The protein ex-pression of TGF-1 and IL-1 0 was detected by ELISA.Results:Periodontitis mice showed an obvious decrease in the percentage of CD4 +Foxp3 + cells in CD4 + T cells in gingival tissues,cervical lymph nodes(CLNs)and peripheral blood(P <0.01 ).Moreover,peri-odontitis mice had an obvious increase in the expression of TGF-1 and IL-1 0 in gingival tissues,CLNs and serum(P <0.05).Conclu-sion:Treg cell-mediated immune response is weakened in the mice with periodontitis.Gingival tissues,CLNs and the peripheral blood are probably the main source of Treg cells during periodontitis.