Objective To discuss nursing of patients with early severe acute pancreatitis undergoing laparoscopic treatment and in comparison with open surgery. Methods Totally 56 patients with early se-vere acute pancreatitis were admitted from October 2002 to October 2007,among which 30 cases (group A) received laparoscopic.treatment and the other 26 cases (group B) was given open surgery.The average time of hospital stay,incidence of complication and re-operation were compared between the two groups. Re-sults The average time of hospital stay,incidence of complication and re-operation of group A were lower than those of group B (P<0.05). Conclusions Treatment of severe acute pancreatitis with laparoscopy proved to be a desirable method in that it performs good effect with little trauma and low fatality.Other basic treatment,regulation of water and electrolyte balance and acid-base balance,enforcement of nutritional sup-port,fasting and gastrointestinal decompression should not be ignored.

OBJECTIVE To study the diagnostic and therapeutic conditions in a general hospital,and try to find out the measures to decrease cross infection in hospital. METHODS From Dec 2003 to Nov 2004,2 037 cases of infectious diseases were diagnosed and treated in our hospital.All these cases were analyzed. RESULTS Among the 2037 cases,the most common diseases were viral hepatitis and pulmonary tuberculosis.And others were infectious diarrhea,gonorrhea,syphilis,typhoid,paratyphoid,epidemic parotitis,bacillary dysentery,epidemic encephalitis B,measles,tetanus of newborn and rabies. CONCLUSIONS To control cross infection in hospital effectively,we must pay more attention to standardized management of infections diseases,strengthen the isolation and protective conciousness of medical personnel for infectious diseases,and tighten up the management of sterilization and isolation.

OBJECTIVEThis study investigates the immune status of T helper (Th) 17 cells in mice with periodontitis.

METHODSSeven-week-old C57BL/6 female mice were randomly divided into the control and periodontitis groups. Each group comprisedfour mice. Experimental periodontitis was induced in mice by oral infection with Porphyromonas gingivalis in the periodontitis group. Phosphate-buffered saline solution was used in the control group. All mice were sacrificed 4 weeks after the last P. gingivalis infection. CD4⁺retinoid-related orphan receptor (ROR) γτ⁺(Th17) cells were analyzed by flow cytometry. The protein expression of Th17 cell-related cytokine interleukin (IL)-17A was detected by enzyme-linked immunosorbentassay (ELISA).

RESULTSFlow cytometry showed that the percentage of CD4⁺RORγτ⁺(Thl7) cells in all CD4⁺ cells and the cell number in gingival tissues, cervical lymph nodes (CLNs), and the peripheral blood obviously increased (P < 0.01) in the periodontitis group. ELISA showed that compared with the control group, the periodontitis group exhibited an obvious increase in the protein expression of IL-17A (P < 0.05).

CONCLUSIONTh17 cell-mediated cell response is enhanced, and the gingival tissues, CLNs, and the peripheral blood are probably the main locations of Thl7 cell-mediated cell response during the development of periodontitis.

Alveolar Bone Loss ; Animals ; Cytokines ; Female ; Flow Cytometry ; Gingiva ; Interleukin-17 ; metabolism ; Mice ; Mice, Inbred C57BL ; Periodontitis ; metabolism ; Porphyromonas gingivalis ; Random Allocation ; T-Lymphocytes, Helper-Inducer

Objective:To investigate whether lanthanum(La 3+)can enter into ossification induced bone marrow stromal cells(BMSCs)cultured in vitro and its influences on the ultrastructure of BMSCs.Methods:Dog BMSCs were ossification induced and the cells of the third passage were cultuerd with the presence of lanthanum chloride(LaCl_3)at 5.564?102,5.564 and 5.564?10-2 ?g/ml respectively for 5 days,the same passage cells without LaCl_3 treatment were used as the controls.Transmission electron microscope(TEM),electron microprobe with wave spectrum(EMWS)and high resolution TEM with energy spectrum(HRTEM)were used to observe the change of BMSCs ultrastructure and the distribution of lanthanum ions in the cells.Results:Electron-dense granules in lysosome were observed in BMSCs treated with LaCl_3 but not in control BMSCs under TEM.Slight denaturation of some organelles was found in both LaCl_3 treated and control cells.EMWS examination showed that there was La element in BMSCs treated with 5.564?102 ?g/ml LaCl_3.La element was not found in all the treated cells under HRTEM.Conclusion:La 3+ at a certain concentration might enter into the ossification induced BMSCs cultured in vitro.

Objective To evaluate the efficacy of endoscopic techniques for corrections of facial contour deformities.MethodsA total of 58 patients(48 women and 10 men)with square face(38),prominent zygoma(12),or crooked noses(8)were treated by endoscopic-assisted technique in our hospital.Results The operation time ranged from 40 to 86 min(mean,65 min),and intraoperative blood loss was 60-120 ml(mean,78 ml).The patients were discharged from the hospital in 3 to 7 days(mean 4)after the surgery,and was followed up for 3 to 24 months.During the period,no one had unexpected fracture,injuries to the mental and facial nerves and vessels,or infection.All the patients were satisfied with the surgical outcomes.ConclusionsEndoscopic-assisted correction of facial contour deformities is feasible,safe,and effective.

Objective:To explore the effects of class career psychological guidance(CCPG)on the career planning abilities of college students.Methods:By random sampling and control study methods,one class of college students were regarded as the experimental class of subjects,the other class was regarded as the control.The experimental class received 3 months of group training of CCPG,while the control class did not.They were assessed with the effect assessment scale of CCPG and the experimental class was retested 3.5 months later.Results:The overall level of career planning abilities had been improved in the experimental class after 3 months of CCPG,such as in the career planning confidence and the ability to solve problems relating to career,not in the control class. Most of the improved factors in the experimental class were still good after 3.5 months.Conclusion:The class career psychological guidance has a positive role in promoting the career planning abilities of college students.

Objective: To investigate the relationship between the ac tive granulae in mast cells and infant hemangiomas of parotid gland. Methods: Surgical samples of parotid gland hemangioma were obtained from 40 infants under the age of 6 months and those of normal parotid gland tis sue from 5.The samples were investigated by safranin staining,alcian blue and th ionine staining, and image analysis technique. Results: Th e percentage of thionine positive cells vs tissue area in hemangioma and normal parotid gland tissue was 1.132?0.195 and 0.335?0.122 ( P

Objective To study the clinical and endoscopic features of Gastric mucosa－ associated lymphoid tissue(MALT) lymphoma. Methods Clinical and endoscopic data of thirty two patients with gastric MALT lymphoma diagnosed pathologically after opration were analyzed. Results Patients with gastric MALT lymphoma had a median age of 51.3years with male predominance and a bimodal age distribution pattern, aged 30～ 39 and 60～ 69 years respectively. The symptoms were not characteristic.The tumor originated from antrum in 12 cases,corpus in 8 and multifocal lesions were found in 12 instances. The tumor appeared infilitrative in 12, ulcerative in 16 and nodular in 6 patients endoscopically. Lymphatic metastasis was detected in infiltrative type, before the serosa was involved. Immunohistochemistry showed B－ cell lymphoma in all the 32 cases.And Helicobacter pylori(H.pylori) infection was found in 27 cases(84.4％ ).Nocorrelation was found between endoscopic types and manifestations.Metastasis to local lymph nodes was found early in infiltrative tumor.Conclusions It is suggested that H.pylori infection may play a role in the pathogenesis of gastric MALT lymphoma.

Objective To establish an HPLC method for determination of matrine in matrine ethosome thermosensitive gels. Methods Matrine was detected in matrine ethosome thermosensitive gels by using a YMC-Pack NH2 column(4.6 mm× 150 mm,5 μm),with the mobile phase containing acetonitrile-ethanol(9: 1).The flow rate was 0.8 mL·min-1 ,the column temperature( 25 ± 2) ℃ and the UV detection wavelength 215 nm. Results The peak area correlated with matrine concentration within the range of 1.00-100.00 μg·mL-1 linearly,and the average correlation coefficient was 0.999 2.The average recoveries rate of low,medium and high concentration of matrine were 98.16%,98.15%,98.00%,respectively,and the RSD were 0.30%,0. 69%,0. 71%,respectively. Conclusion The method is simple,sensitive and accurate. With the advantages of specificity,high precision,good stability and repeatability,the HPLC method can be used for the determination of matrine in matrine ethosome thermosensitive gels.

Objective:To probe the application and the effect of integration medical health education mode in the care-givers of children with capillary bronchitis. Method:Total 120 cases of capillary bronchitis caregivers were divided into control group (56 cases) and observation group (64 cases) and control group with traditional mode of health education, observing group adopts the integration medical health education mode;Compared two groups of children with hospitaliza-tion days, take care of health education knowledge of cooperation degree, satisfaction and health care work. Results:After implementation the integration medical health education mode, children hospitalization days declined. Caregivers' health education knowledge and satisfaction were obviously improved. The medical work cooperation degree is higher than the control group. Conclusions:The implementation of the new mode is helpful to improve work efficiency, reduce the finan-cial burden on families, and meet the demand of caregivers, health education and the right to know and sublimation of medical humanism philosophy, promoting the comprehensive qualities of nurses, improve patient satisfaction, harmonious relationship between hospital and patients, improve high quality nursing care for patients.