Objective To study the clinical characteristics, the outcome of pregnancy and the risk factors of uteroplacental apoplexy complicating severe placental abruption. Methods A retropectively study of the 52 cases of placental abruption who had delivered in our hospital from Jan. 2002 to Dec. 2006 was conducted. These cases were divided into 2 groups: 17 cases of uteroplacental apoplexy complicating placental abruption as observation group, the others with no uteroplacental apoplexy as control group. The risk factors of disease, clinical characteristics and the outcome of pregnancy between the two groups were compared. Results (1)The incidence of placental abruption was 0. 15% (52/35 049) among the total deliveries patients with uteroplacental apoplexy complicating placental abruption took up 0. 05% (17/35 049) of all deliveries and 33% (17/52) of all abruption cases. (2) General information and delivery : There were no significant differences ( P > 0. 05 ) regarding their mean age and BMI in two groups. All women in observation group had C-section delivery, which were 21 in control group. 14 women had vaginal delivery. The incidence of premature labour was 88% ( 15/17 ) in observation group, and 49% (17/35 ) women in control group delivered after 37 weeks. Significant differences were observed regarding delivery methods and gestational weeks(P <0. 01 ). (3)Risk factors: the incidence of preeclampsia, 71% (12/17), and the duration of disease, 6. 4 hours, in observation group were more than those in control group, 20% (7/35) and 4. 2 hours( P < 0.01 ). There were no significant differences between two groups in premature rupture, polyhydroamnions ( P > 0. 05 ). (4) Clinical characteristics in two groups : bloody amniotic fluid, fetal distress, hematometra and postpartum hemorrhage occurred in 82% (14/17) vs 26% (9/35), 65%(11/17) vs 29% (10/35), 35% (6/17) vs 6% (2/35), and 59% (10/17) vs 11% (4/35), with a significant difference (P <0. 01), but no statistical difference existed between indices such as abdominal pain, vaginal bleeding and abdominal tension ( P > 0. 05 ). (5) Placenta sites and abruption areas: placenta sites were distributed from anterior or posterior of uterine body 5/17 vs 24/35 , the fundus or cornu of uterus 12/17 vs 11/35 ( P < 0. 01 ). All cases in observation group presented abruption areas> 1/3, and 9 cases ≥2/3, 27 cases abruption areas < 1/3 and 8 cases abruption areas 1/3 -2/3 in control group (P<0.01). (6) Other complications and outcome: Hemorrhagic shock 3 vs 0, DIC 3 vs 0, hysterectomy 1 vs 0, intrauterine fetal death 3 vs 2, neonatal asphyxia 8 vs 5 and neonatal death 1 vs 0. There were significant differences ( P < 0. 01 ) between the two groups. Conclusions Preeclampsia, long duration of disease and fundal or cornual placenta a risk factors for uteroplacental apoplexy complicating placental abruption, which may lead to a poor maternal-fetal prognosis.

Objective:To investigate the alveolar bone height change before and after retraction of anterior teeth in adult patients. Methods:A total of 15 adult patients with angle class I bimaxillary protrusion had 4 first premolars extracted followed by othodontic treatment for retraction of anterior teeth with straight arch wire techniques.Cone beam computed tomography(CBCT)scans were made before and 3 months after retraction.The labial and the lingual alveolar bone height around central and lateral incisors was measured and analyzed before and after treatment.Results:Significant increase of labial alveolar bone height around anterior teeth was found in both maxilla and mandible(P ＜0.05),however,lingual alveolar bone height was decreased(P ＜0.05).Conclusion:For bimaxillary protrusion patients,the lingual alveolar bone height decreased due to obvious incisors retraction in adults which should be considered carefully in orthodontic clinic.

Objective To explore the application of the comprehensive pain assessment scale to the treatment of postherpetic neuralgia (PHN). Methods About 100 patients suffering from PHN were randomly divided into two groups: the control group and experiment group. The former was treated with recording for pain assessment and the latter was treated with the comprehensive pain assessment scale. Two sets of data were compared and analyzed in view of quality of sleep, average length of hospitalization and the duration of side effects of drugs. Result The effect of the experiment group was better than the control group in the quality of sleep, the average length of hospitalization, reduction of side effect duration of drugs (P<0.05). Conclusion The comprehensive pain assessment scale throughout the whole process of the PHN treatment can achieve timely and effective assessment of pain and reduce the side effect duration of drugs, improve their sleep quality, and shorten the hospital stay.

90 extracted single rooted human teeth were inoculated with E.faecalis.The cannals of the teeth were irrigated with MTAD, 5.25% NaClO,compound chlorhexidine gargle,tinidazole mouthwash,distilled H2 O(infection control)and autoclave followed by distilled H2 O(autoclaving control),respectively(n =1 5).Then E.faecalis infection of the samples were examined.The results showed that MTAD had significantly greater antibacterial effects than 5.25% NaClO,tinidazole mouthwashes,compound chlorhexidine gargle and tinidazole mouthwash(P <0.05).There was no significant difference between each 2 of the other 3 irrigants.

Ethylene Dichlorides ; poisoning ; Humans ; Intracranial Pressure ; drug effects ; Occupational Exposure ; adverse effects

Objective To assess the osteogenic ability after co-culture BMSC and ADSC in vivo and in vitro.Methods ADSC and BMSC were obtained by adherent screening method and enzymatic digestion method.Flow cytometry was used to confirm the phenotypes of ADSC and BMSC.Oil red O was used to induce MSC to fat.Alkaline phosphatase (ALP) and alizarin red staining were used in osteogenic group.This sample was divided into four groups,no-induced stem cells group;BMSC osteogenic induction group;ADSC osteogenic induction group;co-culture of BMSC and ADSC osteogenic induction group.ALP activities and Calcium absorbance were determined during different periods of osteogenic introduction.OCN and Runx2 expression level were tested via RT-PCR and western blot methods after osteogenic induction for 2 weeks.Furthermore,cells in each group were seeded on HA/CS/PLLA composite scaffolds,and the scaffolds with cells were planted into bone defects in rat models.The rats were sacrificed by overdose anesthesia at 8 weeks after surgery and the scaffolds were removed for further analysis.Results Oil red O staining demonstrated red after adipogenic induction.Alkaline phosphatase and Alizarin red staining showed flaky red under condition of osteogenic induction.There had no statistical change among each group after osteogenic induction for 3 days,and ALP activity significantly increased after osteogenic induction for 5 days.Meanwhile,the ALP activity in co-culture of BMSC and ADSC group was markedly higher than the other three groups.However,there had no significant change in A value of calcium absorbance among each group after osteogenic induction for 7 days,while it increased at 14th day and ALP activity in co-culture of BMSC and ADSC group was significantly higher than the other three groups.After osteogenic induction for 2 weeks,the mRNA expression of OCN and Runx2 in co-culture of BMSC and ADSC group was 78.24±8.11 and 1 180.13±121.16 respectively,and the protein expression of OCN and Runx2 was 6.54±0.59 and 4.43±0.51.These mRNA and protein expression level in co-culture of BMSC and ADSC group enhanced significant compared with the other 3 groups.Histological assay demonstrated that the new bone tissues formed in co-culture of BMSC and ADSC group were 497.75±7.44 μm2,which was larger than that in the other 3 groups at 8 weeks after implantation.Conclusion Co-culture BMSC and ADSC may up-regulated the osteogenic ability in vivo and in vitro.

Objective To study the spatiotemporal distributions of epithelial proliferation and apoptosis,and the correlation of morphogenesis with them in developing stomach.Methods Serial sections of fetal mice aged 11-15.5 days were made.The morphogenesis of stomach was observed under light microscope.The density of mitotic cells(DMC) and apoptotic bodies(DAB) of epithelium in the developing cardia,pylorus,greater curvature,lesser curvature were measured with stereological method.Results The morphological development of stomach took place on the 11~(th) day of mouse embryo development.The fundus,greater curvature and lesser curvature were observed on the 12~(th) day.The peak value of DMC all appeared before the peak value of DAB.In the epithelium of cardia,no obvious peak value of DMC was observed and the peak value of DAB was on the 11.5~(th),12.5~(th),13.5~(th) day.In the epithelium of pylorus,the peak value of DMC was on the 12.5~(th) day,the peak value of DAB was on the 14~(th) day.In the epithelium of greater curvature,the peak value of DMC was on the 11.5~(th) and 12~(th) day,the DAB value was low.In the epithelium of lesser curvature,the peak value of DMC was on the 11.5~(th) day,the peak value of DAB was on the 12.5~(th) day.Conclusion There was very close spatiotemporal relationship between the cell proliferation,cell apoptosis and the morphological development of stomach.

Objective: To investigate the adhesive characteristics of two kinds of light-curing resin that was used to bond teeth and orthodontics brackets. Methods: A total of 60 premolar teeth were randomly divided into six groups, and brackets were bonded according to the manufacturers' instructions. In group A1, A2 and A3, the teeth were prepared using 100 ml/L polyacrylic acid, and the brackets were bonded using Fuji Ortho LC. In group B1, B2 and B3, the teeth were using self-etching primer, and the brackets were bonded using Transbond Plus Self Etching Primer (TPSEP). After 30 min and 24 h under room temperature and received cold-heat cycle treatment respectively, bond strength was measured using a testing instrument (2000S, Lloyds Instruments, Fareham, England) at a speed of 1 mm/min, and the residual adhesive was quantified using a stereomicroscope. The adhesive interface was observed by scanning electron microscope. Results: The bond shear strength of TPSEP and Ortho Glass LC are more than 5 MPa. The bond shear strength of TPSEP is stronger than that of Ortho Glass LC after 24 h. However,The adhesive remnant index (ARI)of TPSEP is higher than that of Ortho Glass LC. Conclusion: Transbond Plus Self Etching Primer and Ortho Glass LC can satisfy the clinic need of orthodontics.

Objective:To elucidate the gene mutation presents in hair follicle cells,just as blood cells,in the aminoglycoside antibiotics induced deaf patients (AAID). Method:Mitochondrial DNA of blood cells and/or hair follicle cells from 8 members of 3 aminoglycoside antibiotics induced deafness families were analysed using PCRSSCP and Alw 26 Ⅰ digestian. Result:Our result showed that a gene mutation at nuleotide 1555 in 12 S rRNA of mitochondrial DNA appeared in blood cells and/or hair follicle cells of 7 subjects among those 8 members. Only a father with normal hearing did＇t exhibit such a mutation. Conclusion:This indicated that hairs could be used as a sample instead of blood to perform gene examination for AAID.

OBJECTIVE: To evaluate the efficacy of mite allergen specific immunotherapy (SIT) to patients of allergic rhinitis. METHOD: A total of 102 patients with mite allergy were recruited into the study. They were randomly divided into two groups: SIT group (n = 51) and ST (symptomatic therapy) group (n = 51). They were given SIT with standardized allergen vaccine for 3 years or only symptomatic therapy respectively. Observation items include: rhinitis symptom scores, drug score, skin prick test result, serum specificity IgE (sIgE), peripheral eosinophil counting. The development of asthma and new allergens sensitization was also assessed. RESULT: The blood eosinophil numbers, skin test index, rhinitis symptom scores and drug scores were all decreased significantly after the treatment with SIT for 3 years compared to that of ST group (P < 0.01). Although the level of serum slgE was decreased, no statistic diferences were found. No patients developed asthma in SIT group, and only 2.1% of patients had new allergen sensitization; 17.4% of those in ST group developed asthma, 32.6% had new sensitization. No severe adverse events occurred. CONCLUSION Keeping long-term SIT is effective and safe for patients with allergic rhinitis induced by mite, which can also prevent new allergen sensitization and development for asthma.
Adolescent ; Adult ; Antigens, Dermatophagoides ; administration & dosage ; Child ; Desensitization, Immunologic ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Rhinitis, Allergic ; therapy ; Treatment Outcome ; Young Adult