Objective To analyze the relationship between volume,conductivity,laser light scatter (VCS) of lymphocytes and monocytes and atypical lymphocyte.Methods The clinical data of 129 patients with virus infection and 50 healthy controls received blood routine examination by Sysmex-800i hematology analyzer in our hospital were retrospectively analyzed.The lymphocyte volume (MLV),distribution width,(LDW),conductance rate (MLC),laser scattering(MLS) and monocyte cell volume (MMV),distribution width,(MDW),electrical conductivity (MMC),laser scattering(MMS) were analyzed.And according to the heterotypic lymphocyte ratio,the virus infection patients were divided into group 1 (abnormal rate 2％-5％),group 2 (abnormal rate ＞ 5％-10％),group 3 (abnormal rate ＞10％).The healthy controls were selected as control group.The VCS parameters of the four groups were compared.Results The LDW of group 1 was (17.2 ± 2.5) FL,that of the control group was (14.1 ± 2.7) FL,the difference was statistically significant(t =2.362,P ＜0.05).The MLV,LDW,MMV,MDW of group 2 were (85.3 ±5.6) ×109/L,(19 ±3.1) FL,(185.9 ±9.2) × 109/L,(22 ±2.9) FL,which were significantly higher than those of the control group (t =2.557,3.086,2.735,4.269,P ＜ 0.05).The MLV,LDW,MMV,MDW of group 3 were (89.5 ±6.8) ×109/L,(22.3 ±3.6) FL,(194.5 ±9.6) ×109/L,(24.5 ±3.4) FL,which were significantly higher than those in the control group (t =2.982,3.569,2.945,4.863,P ＜ 0.05).The MLC,MLS,MMC,MMS of group 1 were (112.3 ±7.8) μs/cm,(54.8 ±4.9) NTU,(112.3 ±7.8)μs/cm,(83.6 ±4.7) NTU,those in group 2 were (104.7 ±6.9)μs/cm,(52.6 ± 4.5) NTU,(104.7 ± 6.9) μs/cm; (81.1 ± 4.3) NTU,those in group 3 were (96.5 ± 6.2) μs/cm,(50.5 ± 4.3) NTU,(96.5 ± 6.2) μs/cm,(78.7 ± 3.8) NTU,those in the three groups were significantly lower than in the control group (t =2.623,3.195,2.247,5.829,2.457,2.956,2.371,6.715,2.573,3.044,4.395,7.386,P ＜ 0.05).Conclusion VCS parameters of lymphocytes and mononuclear cells of two types of white blood cells can reflect to a certain extent,peripheral blood heterotypic lymphocyte morphology changes of clinical infection,has the reference value of diagnosis and treatment of disease.

Critical Illness ; therapy ; Humans ; Nutritional Support

Gait problems in cerebral palsy patients are usually caused by spasticity or contracture of two joints muscles,and the imbalance between synergic muscles and antagonistic muscles. The knee problems are the most common seen in cerebral palsy patients. The paper discusses the common abnormalities of the knee in cere-bral palsy and the development in treatment of the deformities.

Program monitoring and evaluation (M & E) is the major method used in the international collaboration programs on disease control and prevention in China in recent years. It functions in the progress overseeing,problem finding and solving in terms of enhancing the program quality,managing the work plan,summing up the experiences,auditing the performance and finally achieving the expected goal. The indicators and implementation progress based on M & E serve as a scientific tool to measure the program implementation outcome periodically.

Objective To investigate the indications and clinical values of percutaneous transthoracic biopsy guided by different locating means respectively on pulmonary lesions. Methods Patients were divided into different groups according the locating methods,which included X -ray, type -B ultrasonography, and CT. Pathologic diagnosis and complications were retrospectively analysed between groups. Conclusion Each locating method has its own advantages and disadvantages. Results we should choose the optimistic one according the patients′ condition.

The responsibility system of attending doctors plays a positive role in raising hospital efficiency and optimizing medical resources deployment.For the change in the subject of medical liabilities following the introduction of this system,medical management needs to shift from department management to doctor management instead.In the meantime,appropriate rules and regulations are needed to normalize the roles and behavior of the medical office,department heads,and attending doctors in medical activities.This will ensure the successful operation of this responsibility system,and generate ideal medical,economic and social outcomes.

Objective To optimize the formulation of controlled-onset extended-release tablets of nicorandilby central composite design-response surface method. Methods The tablets containing nicorandil were prepared bydry-compression coating technique.The influence factors included the amount of HPMC, the amount of EC, and the ratio of lactose/MCC in coating film. The evaluation parameter was Q4 h and Q5 h . The possibly optimal formulation was predicted by response surface method. Results The OD was simulated using second-order polynomial equation and the r2was 0.970 1.Optimal prescription was as follows:HPMC 89.96 mg, EC 46.21 mg, Lactose/MCC 1.87.Bias between the observed and predicted values of the OD was within 0.43%. Conclusion The controlled-onset extended-release tablets of nicorandil can release drug quickly in vitro at the predetermined time.Central composite design-response surface method can be used to optimize the formulation and the model developed in this study was proved highly predictable.

OBJECTIVE: To evaluate the current situation and the trend of cardiovascular drugs used in 6 cities in the Yangtze river valley between 2002 and 2004.METHODS:Consumption of cardiovascular drugs used in 157 hospitals of 6 cities in the Yangtze river valley were analyzed by the way of sum and DDDs ranking. RESULTS:The total annual cost of the cardiovascular drugs used in 6 cities was 13.6 billion yuan in 2004, increasing by 58.8%, as compared with that in 2002.The total average consumption of anti-anginal and vasodilating agents, antithrombotic agents and antihypertensives accounted for 84.1% of the total cost in 3 years.CCB, ACEI, Nitrate esters,?-blockers and Chinese medicines dominated in the ranking of sum and DDDs.CONCLUSION: Right medicines with higher patient compliance rates, lower ADR occurrence, more certain therapeutic effect and economical daily cost have become the preferable choices for clinical physicians.

Objective To explore the effect of DNA methylation inhibitor 5-aza-2'-deoxycytidine (5-Aza-CdR) on proliferation and tumor suppressor gene PRDM1α expression in Burkitt's lymphoma cell line NAMALWA. Methods MTT was used to study the impact of DNA methylation inhibitor on proliferation in NAMALWA cells,and comparative real-time reverse transcription-polymerase chain reaction (RQ-PCR) with SYBR Green assay was used to detect PRDM1α gene expression. Results MTT results showed that 5-AzaCdR inhibited proliferation of NAMALWA cells in a dose-dependent manner.After treated with 5-Aza-CdR for 72 h at 0.01,0.0625,0.1,0.125,0.25,0.5,1,2 and 4 μmol/L,the inhibition rates were 21.93 ％,39.23 ％,47.69 ％,50.37 ％,53.54 ％,57.72 ％,62.31％,65.68 ％,67.87 ％,respectively,and the differences of absorbance among the groups were also statistically significant (all P＜ 0.01). Meanwhile, 5-Aza-CdR could induce the re-expression of PRDM1α, and △Ct of 0.5, 1, 2 μmol/L groups showed significant differences compared with control group (all P ＜ 0.05). Conclusion DNA methylation inhibitor 5-Aza-CdR can significantly inhibit the proliferation of Burkitt' s lymphoma cell line NAM ALWA,and the possible mechanism of this inhibition may be induction of demethylation and re-expression of PRDM 1α.

Objective: To monitor the blood concentration of high-dosage of methotrexate (HD-MTX) during chemotheraphy in the treatment of patients with osteosarcoma and discuss its clinical significance. Methods: Thirty-five patients with osteosarcoma who received high dosages of MTX (6 g/m2) for 50 times were involved in our study. The serum concentration of MTX was determined at each time point by improved RP-HPLC. The relationship between the serum concentration of MTX and the adverse reaction was analyzed. Results: The mean peak MTX level was (348.60 ± 200.47) μmol/L. The serum concentrations of MTX were significantly higher at 24 h and/or 48 h than safe levels (105 μmol to 106 μmol) in 15 cases (30%). The incidence rates of adverse reactions such as neutropenia, oral mucositis and rash were 40.0%, 33.3%, and 20%, respectively. Conclusion: There is significant variability in the MTX serum levels. The incidence rates of ad reactions are relatively higher in those patients whose 24-h and/or 48-h serum MTX concentrations are higher than the safe levels.