Objective To explore the clinical nursing path teaching method application in children with mycoplasma pneumonia and its effect.Methods 93 higher vocational nursing students of two classes of Grade 2011 were chosen as the subjects,one class as the experimental group(46),and the other class as the control group(47).The traditional teaching method was used in the control group,while method of clinical nursing path was used in the experimental group.Two groups of nursing students were given closed book exams and questionnaires to evaluate teaching effectiveness of the two groups.SPSS 13.0 was used for statistical analysis,count data using x2 test,measurement data using t-test,test level α=0.05.Results The experimental group students' test scores were significantly higher than the control group(85.26 ± 3.57)vs.(76.39 ± 4.16),and the difference was statistically significant P=-0.02.The survey showed that there were more students in the experimental group than in the control group who made a positive comment on the questionnaire's role in helping improve students'learning autonomy,their critical thinking ability,and promote their grasp of the content of the disease.Conclusion Clinical nursing path teaching method as a new kind of teaching method,can improve the teaching effect.

Health history,clinical syndrome and imaging examination are key factors for early diagnosis of pancreatic trauma,while the effects of the test of the serum amylase for the diagnosis of pancreatic trauma remain unclear.Laparotomy plays an important role in the diagnosis of pancreatic trauma for patients,particularly urgent patients.The grading of pancreatic trauma functions as a bridge between the diagnosis and treatment of the pancreatic trauma.So far,the organ injury scale grading system proposed by the American Association for the Surgery of Trauma (AAST-OIS) provides guidelines for operative versus nonoperative management in solid organ injuries.Surgery is an important treatment method for pancreatic trauma according to injury control and surgical procedures grading principles.Recently,minimally invasive surgery has been involved in the diagnosis and treatment of the pancreatic trauma.

ObjectiveTo investigate the influencing factors of preoperative fearful anxiety,and explore the effect of nursing intervention for the improvement of preoperative fearful anxiety. Methods500 cases of surgical patients in our hospital from January 2009 to January 2010 were chosen as the research objects.They were randomly divided into the control group and the observation group with 250 patients in each group.The control group was given routine preoperative nursing care.The observation group was given preoperative nursing intervention.The general information,surgery program,knowledge of surgical program,the patients' family situation and economic situation were taken for statistics.Their SAS score 30 minutes before the surgery was determined,and the influencing factors of preoperative fearful anxiety were analyzed. ResultsThe study showed that the influencing factors of fearful anxiety included:character,education background,family economic status,knowledge of the situation of surgery and other factors.The anxiety score 30 min before surgery was compared,the observation group was significantly lower than that of the control group.The difference between the two groups was significant. ConclusionsThe preoperative fearful anxiety was directly related to patients' own situation and level of medical services.Fully understanding of the situation before surgery and targeted preoperative nursing interventions can effectively reduce preoperative anxiety,and is conducive to the successful implementation of operation and postoperative rehabilitation.

Objective To investigate the psychological state and its correlation factors of family members of elderly cancer patients. Methods A total of 76 family members of elderly cancer patients were investigated with questionnaires with Zung's self-rating anxiety scale (SAS) and self-rating depression scale (SDS). Their psychological state and its correlation factors were analyzed. Results Incidence rate of anxiety of elderly cancer patients' family members was 52.63%, and incidence rate of depression was 46.05%. The mean standard score of SAS and SDS of elderly cancer patients' family members were both significantly higher than domestic norm. Difference of family member's gender, ways to pay health care fee, pain degree and disease condition led to different SAS and SDS score. Conclusions Family members of elderly cancer patients possessed different degree of psychological obstacle which related with multiple factors. Medical staff should not only think highly of patients' psychological state, but also their family members'. And meanwhile they should take corresponding strategy to reduce the incidence rate of anxiety and depression state in those family members.

This article discussed the reform of library cataloguing induced by library standardization and automation from four aspects of expansion of cataloguing scope, the change of cataloguing methods, deep revealing of literature cataloguing, and the elevation of personnel quality.

Objective To evaluate the clearance effect of hemodiafihration(HDF) and hemopeffusion combined with hemodialysis( HP + HD) on serum calcium,phosphorus and parathyroid hormone in patients with uremia.Methods Fifty-eight uremia patients treated with maintenance hemodialysis in our hospital from May 2009 to October 2010 were randomly divided into two groups:HDF group (n =29)and HP + HD group (n =29).Patients in HP + HD group underwent HD conventional treatment 5 times and HP + HD.treatment once every two weeks,while those in HDF group underwent HD treatment 5 times and HDF treatment once every two weeks.All subjects were treated for 12 weeks.Serum calcium,phosphorus and parathyroid hormone were detected and analyzed before and after the treatment.Results After therapy,the serum calcium ( [ 2.41 ± 0.32 ] mmol/L v.s.[ 2.22 ± 0.35 ] mmol/L,t =5.47,P ＜ 0.01 ),phosphorus( [ 1.98 ± 0.34 ] mmol/L v.s.[ 1.70 ± 0.31 ] mmol/L,t=8.13,P ＜0.01)and parathyroid hormone( [645.19 ± 184.52] ng/L v.s.[296.79 ± 103.54]ng/L,t =6.54,P ＜ 0.01 ) levels of HP + HD group decreased significantly.Similarly,the serum calcium ( [ 2.43 ± 0.33 ] mmol/ L v.s.[ 2.32 ± 0.37] mmol/L,t =6.72,P ＜ 0.01 ),phosphorus ( [ 1.97 ± 0.36 ] mmol/L v.s.[ 1.83 ± 0.35 ] mmol/L,t =6.47,P ＜ 0.01 ) and parathyroid hormone ( [ 639.47 ± 208.73 ] ng/L v.s.[ 409.91 ± 152.72 ] ng/L,t =5.27,P ＜ 0.01 ) levels of HDF group also decreased significantly.Comparison of the two blood purification methods indicated that the clearance effect of HP + HD on serum calcium,phosphorus and parathyroid hormone was significantly better than HDF( t =4.24 and t =7.24,P ＜ 0.01 ).Conclusion Both HDF and HD + HP could clear serum calcium,phosphorus and parathyroid hormone effectively,while the clearance effect of HD +HP is better than HDF.

Objective To study the effects of sucking sputum with face upwards and neck raised in order to supply a new method for sputmn sucking.Methods One hundred and twenty-three patients were randomly divided into the experimental group(n=61)and the control group(n-62).The experimental group adopted modified mode:cleaned up the sputum within the mouth and nasal cavities with the traditional manner firstly,then the patients lay down,the nurses raised the patients'neck softly with one hand,removing the pillows,while putting the hypothenar of the other one on his forehead,kept the airway open to suck sputum.The control group adopted common mode,but using the modified method when the common mode failed.Results There was significant difference in phlegm sucking effect between the two groups(P＜0.01).Besides,the first-time success rate d tube insertion,incidence of trachcotomy,mucosal injury and hemorrhage and satisfaction degree of patients'family were statistically different between the two groups(P＜0.01).Conclusion Sucking sputum with face upwards and neck raised waS superior to the traditional one.

Objective:To study the effect of substance P on sensation afferent of gastrointestinal tract and the corresponding mechanism. Methods:Forty SD rats were divided into 5 groups:control group,low dosage SP group(75 ng/kg),middle dosage SP group(150ng/kg), high dosage SP group(300 ng/kg)and spantideII+SP group.The electrophysiological method was used to record the change of spontaneous and gastric distention-induced afferent discharge of subdiaphragmatic vagus nerve in each group.Results:Intravenous SP (150 ng/kg,300 ng/kg)caused significantly increasing effect on the spontaneous afferent discharge(P

Objective:Toinvestigate the etiological agents of the outbreak ofhand-foot-mouth disease(HFMD)in ChongQing area from April to September in 2009.Methods:100 specimens(including cerebral spinal fluid,vesicle fluid,stool,throat swabs,anal swab) were collected from 47 patients with HFMD during the epidemic season.The specimens were inoculated into RD cells for isolation of enteroviruses(EV),The supernatant of cytopathic effect(CPE)were detected by RT-PCR with universal primers of enterovirus, genus-specific primers of EV71 and genus-specific primers of Coxsackie A16,and the amplified products were sequenced to determine the outbreak of the pathogen enteroviruses(EV),The sequencing results will be compared with international representatives BrCr strain to identify the enteroviruses(EV)by Bioedit biological software.Results:In the 100 cell cultures of clinical specimens of children HFMD, apparent Cytopathic were observed in 7.The results of RT-PCR showed that seven specimens were positive with intestinal universal primers,and three were positive results with EV71-specific primers,which were from two cases of out-patient children and one with clinical diagnosis of suspected case,while the negative results were obtained with CA16-specific primers for all the samples.The recovery of the PCR products were cloned and the sequencing identification confirmed as EV71 virus,while sequencing analysis of homology-related in the results.Conclusion:EV71 were the major etiological agents of the HFMD outbreak in Chongqing from February to April in 2009.

Objective:To explore the effect of polycylic aromatic hydrocarbons (PAHs)on plasma monoamine neurotransmitters. Methods:80 healthy adult male coke oven workers were selected from a coke plant of a state-owned steel enterprise,80 controls were selected from the oxygen factory. Concentration of benzo[a]pyrene(B[a])P in the working environment was monitored by high performance liquid chromatography(HPLC)with fluorescence detector. The level of urinary 1-hydroxypyrene(1-OHP)was determ ined by HPLC with fluorescence detector.The plasma noradrenaline(NE),adrenaline(AD),dopamine(DA)and serotonin(5-HT)levels were measured by high performance liquid chromatography (HPLC) with electrochemical detection. Results:In the working environment of B[a]P, concentration in coke oven workers were significantly higher than controls (P