Objective To explore the experience of nursing the patients with hypoparathyroidism.Methods Five pregnant patients with hypoparathyroidism hospitalized in our hospital during January 2009 to December 2015 were included in the study.The nursing intervention included performance of health care during pregnancy,childbirth and postpartum nursing.Results The thyroid function of the 5 patients became normal after medication and delicate care.All underwent cesarean resection and all the infants were normal in terms of thyroid function.Conclusions Pregnancy combined with hypoparathyroidism is of high risk and harmful for mothers and neonates.So they should be under management of high risk pregnancy.The prenatal,intrapartum and postpartum nursing measures should be implemented to ensure the safety of delivery.

Critical Illness ; therapy ; Fluid Therapy ; Humans

Objective To construct vectors based on adeno-associated virus type 2 (rAAV2) carrying bone morphogenetic protein-7 (BMP-7) and observe their expression in adipose-derived adult stem cells, which can be served as a new gene therapy method and cell source for bone tissue engineering. Methods The coding sequence (1. 3 kb) of BMP-7 was amplified by PCR from the pcDNAl. 1 ( + ) plasmid containing the human BMP-7 cDNA. After purified, the gene fragments were cloned into a plasmid pUC18 and termed plasmid pUC18-hBMP7. The recombinant pUC18-hBMP7 was digested and further ligated to the pSNAV by T4DNA ligase and termed plasmid pSNAV-hBMP7. BHK-21 cells were transfected with the purified pSNAV-BMP7 plasmid according to a standard calcium phosphate precipitation method. The cells were isolated and the integrity of hBMP7 gene was determined by polymerase chain reaction (PCR) using the above PCR primers. To package the virus, stably transfected BHK-21 cells were subsequently infected with recombinant herpes simplex virus type 1 (rHSV-1). The collected cells were processed by chloroform treatment, PEG8000/NaCl precipitation and chloroform extraction for purification. The tiler was determined using quantitative DNA dot blots and the purity was examined by sodium dodecyl sulphatepolyacrylamide gel electrophoresis. Following infection with rAAV2-BMP7 at multiplicities of infection of 1?105 vector genomes per cell and subsequent culture, adipose-derived adult stem cells were assessed qualitatively for BMP7 production. Results The recombinant plasmid pSNAV-hBMP7 was identified by PCR and digested with restriction enzyme. Transfection showed an efficiency of 90 % in ADAS cells. BMP-7 expression in ADAS cells was identified by Western blot. Conclusions The hBMP-7 recombinant adeno-associated virus vectors can be successfully constructed in vitro and rAAV2-hBMP7 can infect ADAS cells.

Objective To present the experience of surgical treatment of 12 patients with advanced carcinoma of esophagus involving aorta. Methods Resection of esophageal carcinoma and cervical esophago-gastrostomy or supraaortic esophago-gastrostomy were done in 12 patients. In all 12 patients, the resection of limited aortic adventitia was performed in 2 patients, excision of aorta and simple suture in 2 patients and aortoplasty with Gore-Tex patch in 7 patients, the resected segment was replaced with artificial vessel in 1 patient. Results Complete recovery was achieved in 2 weeks in 12 patients, without severe complications such as aortic fistula. Conclusion Surgical treatment expands the operative indications for patients suffering from esophageal carcinoma involving aorta and improves their life quality.

Objective To investigate the status quo of nutrition knowledge,attitude and practice(KAP) among elite female small ball athletes of Guangdong province and its influencing factors.Their serum ferritin was also observed to explore its correlation with their KAP,so as to provide evidences for formulating and implementing scientific and reasonable nutrition education schemes.Methods Fifty-four female small ball athletes,including 20 handball,20 hockey and 14 softball players,were selected to complete a self-designed diet questionnaire based on KAP education mode and their serum ferritin indicators were analyzed at the same time.Results (1)The average scores(all out of 100)of nutrition knowledge (K),attitude (A) and practice (P) were 43.64 ± 11.66 (with a failure rate of 90.7％),78.37 ± 10.21 (with the rate of excellence of 59.3％)and 63.10 ± 10.17(with a passing rate of 37.0％),and the average KAP score was 185.10 ± 23.01,with a passing rate of 44.4％.(2)The one-way ANOVA found that there were significant differences in the average A score and the total KAP score (P＜0.05) among the athletes of different small ball teams.The correlation analysis results showed that the average A score was of significant positive correlation to the average P score.Moreover,the average KAP total score was of significant correlation with sports events (P＜0.05) and the education level (P＜0.05),but not with the age,training period and exercise level(P＞0.05).The multiple stepwise regression analysis results showed that training period(P＜0.05) and sports events(P＜0.05) was the influencing factor of nutrition knowledge and attitude respectively,and that education level (P＜0.01) was the influencing factor of both nutrition practice and KAP.(3)Significant differences were found in the serum ferritin among athletes of different sports events(P＜0.05)the serum ferritin was of significant positive correlation with the nutrition knowledge(P＜0.05).Conclusion (1)The lack of sports nutrition knowledge and the unbalance of nutrition behavior are common in elite female small ball athletes,but they had a positive attitude.It is necessary to carry out a series of effective nutrition education programs to improve their sports nutrition knowledge,cultivate their good nutrition attitudes and finally formulate rational and effective nutritional intervention measures to promote the formation of healthy nutrition behavior of athletes.(2) The sport event is the key influencing factor of the nutrition attitude and dietary behaviors,which suggests that more more attention should be paid to athletes' dietary management and focus should be put on their diary behavior on no-training days and on their energy ratios control of each meal,especially breakfast and extra meals,and rational intake of snacks.(3)The incidence of iron deficiency is consistent with KAP questionnaire survey results,which indicates that it may be an effective measure to prevent iron deficiency anemia through strengthening athletes' dietary nutrition education.

The application of resonance charging circuit to electro-hydraulic impulse apparatus for treating wastewater is introduced in this paper.The circuit permits the graduating increase of current without influence from time.The unique change of voltage on capacitor is favorable for the recovery of electric strength of electro-hydraulic impulse apparatus.The theory and design method of resonance charging circuit are provided.

OBJECTIVE To study the carbapenemase genotypes among Acinetobacter calcoaceticus isolates infected after liver transplantation. METHODS The 5.4 software was used to analyze the resistance of Acinetobacter species. Eight strains of imipenem-resistant A. calcoaceticus which were resistant to multiple drugs were collected. Analytical isoelectric focusing electrophoresis was used to measure the pI of the ?-lactamase. The homology of the isolates was determined by pulsed field gel electrophoresis(PFGE). blaTEM, blaSHV, blaIMP, blaVIM, blaOXA Genes for resistant isolates were amplified and sequenced. RESULTS Imipenem-resistant A. calcoaceticus infected after liver transplantation was resistant to multiple drugs. All of 8 strains were produced OXA-23 carbapenemases (pI of 6.7). The results of PFGE indicated there was a trend of resistant clone transmisstion in the transplantation ward. CONCLUSIONS There is a transmisstion of A. calcoaceticus producing OXA-23 carbapenemase in the transplantation ward of our hospital. It is important to monitor powerfully the bacterial resistance to control infection more effectively.

Objective To summarize and report the experiences of surgical treatment of infective endocarditis. Methods From March 1995 to March 2004, surgical operations were performed in 25 patients with infective endocarditis, 9 of who received emergency surgery and 16 received selective operation. Replacement of mitral valve was performed in 3, aortic valve replacement in 13, mitral and aortic valve replacement in 8, Bentall procedure in 1.Associated cardiac lesions included patent ductus arterioles in 1, ventricular septal defect in 2, rupture of Valsalvaps sinus in 1. Results There was one postoperative death, which gave an overall hospital mortality of 4%. Twenty-four patients were cured and improved. Conclusion Surgical operation should be the preferred mode of surgical correction for infective endocarditis. The timing of surgery and proper perioperative management are key factors for the successful treatment of infective endocarditis.

Objective To investigate the primary angle-closure glaucoma iris weeks after cutting holes cut the width of the near future, long-term effect relationship, selected after close to long-term efficacy of the forecasting methods to improve iridectomy success rate. Methods 90 cases (116 eyes) with primary angle-closure glaucoma were randomly divided into three groups:A group(the width of the iris-week cut-hole cut 1.5mm) ,30 cases 42 eyes;B group(removal of the width of the iris weeks Ceccon 2 mm) ,30 cases 40 eyes;C group(the width of the iris-week cut-lole cut 3 mm) ,30 cases 34 eyes,after over 1 month,they were followed up to observe the intraocular pressure,anterior chamber angle width of the depth, depending on the features, the iris in recent weeks, as well as cutting holes,long-term concurrency disease. Results A:recent surgery success rate of 85.79% ,long-term surgical success rate of 76. 19% ;B group of recent surgery success rate of 87.5% ,long-term surgical success rate of 82.5% ;C group of recent surgery success rate of94.11% ,long-term surgical the success rate of 88.23%, X2 test the difference was significant(P＜ 0. 05) ;3 postoperative IOP decreased in varying degrees compared with the preoperative differences were significant(P＜0.01) ;peripheral angle after deep Random mean :A group of ≥ 1/3CT,compared the difference between before and after surgery were significantly( P ＜ 0.01 ) ;B group and C group ＞ 1/2CT,2 groups were compared before and after surgery the difference was highly significant( P ＜ 0.05 ). Conclusion Iridectomy was the treatment of primary angle-closure glaucoma safe and effective method of cutting holes. The width of the iris weeks to 2～3 mmwas appropriate, beneficial to control the iris weeks before the adhesion and angle-cut hole shallow narrowing.

Objective To detect the microvessel density(MVD) marked by MIF protein in esophagus squamous cell carcinoma(ESCC), and to see if a relationship exists between microvessel density(MVD) and clinicopathologic features. Methods The expression of MIF in vascular endothelial cell in forty ESCC specimens was examined by immunohistocbemistry staining, calculated the mean of MVD and analysed the relationship with clinicopathologic features. Results The positive rate of MIF protein was 95.00 %(38/40) in ESCC vascular endothelial cell. Compared with the normal esophageal squamous tissue[32.50 %(13/40)], there was significant difference in statistics, the mean of MVD was 26.5±8.5. MVD was positively correlated with the tumor TNM stages and lymph node motastasis. Conclusion MIF can be used in tagging vascular endothelial cell, MVD of ESCC was positively correlated with the tumor TNM stages and lymph node metastasis. It might be an major prognostic factor in ESCC.