Objective: To study the changes in radiosensitivity and cell cycle distribution of HeLa cells after inhibition of one or several DNA double-strand break (DSB) repair proteins by small interfering RNA (siRNA) and LY294002. Methods: Ku80 silenced cells (HeLa/Ku8O-siRNA) and control cells (HeLa/ Neg-siRNA) were transfected with siRNA targeting DNA-dependent protein kinase catalytic subunit (DNA-PKcs) or pretreated with 50 μmol/L LY294002, a chemically specific inhibitor of DNA-PKcs. After the cells received 6MV X-ray irradiation, the radiosensitivity of the cells was detected by clony formation assay and cell cycle distribution was analyzed by flow cytometry. Results: The survival fraction at 2 Gy (SF2) value was 0.08 ± 0.01 for HeLa/Ku80-siRNA cells after being transfected with DNA-PKcs siRNA; the SF2 value reached 0.03 ± 0.01 when HeLa/Ku80-siRNA cells were pretreated with LY294002. Both of them were significantly lower than that of untreated HeLa/Ku80-siRNA cells (0.20 ± 0.05). The cells in all the groups were arrested in G2/M phase after irradiaton with 6 Gy X-ray. The G2/M arrest occurred slowly in the DNA -PKcs-siRNA-transfec ted HeLa/Neg-siRNA cells and LY294002-pretreated HeLa/Ku80-siRNA and HeLa/Neg-siRNA cells, which did not reach the peak at 72 h post-irradiation. The G2/M accumulation was maximal at 48 h post-irradiation in other cell lines. Conclusion: Based on 95% inhibition of Ku80 protein, DNA-PKcs or ataxia-telangiectasia mutant (ATM) gene could compensate the DSB repair function. Co-inhibition of these proteins led to increase in radiosensitivity of HeLa cells; Ku80, DNA-PKcs and ATM play different roles in G2/M arrest.

Objective: To explore the correlation between the expressions of DSB (DNA double-strand break) repair protein (including Ku80, DNA-PKcs, and ATM) and radiosensitivity parameters of human tumor cell lines, and to reveal the value of the three proteins for the prognosis of the radiosensitivity of tumor cells. Methods: Eight tumor cell lines were selected including four human cervical carcinoma cell lines (HeLa, SiHa, C33A, and Caski), three human breast carcinoma cell lines (MCF-7, MDA-MB-231, MDA-MB453), and one human lung carcinoma cell line (A549). The expressions of Ku80, DNA-PKcs and ATM protein were measured by Western blotting. The apoptotic ratio of tumor cells was analyzed by flow cytometry after 48 h X-ray irradiation at 10 Gy of 6 MV. SF2 value (survival fraction at 2 Gy) and α and β values were obtained by clone formation assay. The correlation of protein expression with SF2, α/β value or apoptotic ratio was analyzed by Pearson linear correlation analysis. Results: The expression of same protein in different cell lines and the expression of the three proteins in the same cell line had significant difference. There was a positive correlation between the expression of DNA-PKcs and SF2 (r=0.723, P=0.043 <0.05). But Ku80 and ATM expression had no correlation with SF2 (P>0.05). The expression of the three proteins had no correlation with either apoptotic ratio or α/ β value (P>0.05). Conclusions: Tumor cells with higher expression of DNA-PKcs protein will have higher radioresistance. The expression level of DNA-PKcs protein in tumor cells may be an indicator for predicting the radiosensitivity of tumor cells.

This study was aimed to observe the clinical curative effect of Thesium Chinese tablets in the treatment of chronic bronchitis (syndrome of phlegm heat in the lung). A total of 100 cases of chronic bronchitis (syndrome of phlegm heat in the lung) were randomly divided into two groups. The control group was treated with anti-inflamma-tion, relieving cough and asthma, phlegm removing and oxygen inhalation, maintenance of water electrolyte balance and other symptomatic treatment. In the treatment group, on the basis of treatments in the control group, Thesium Chinese tablets were given at the dosage of 1.6 geach time, 3 times a day. Both groups were treated for 1 course of 10 days. The results showed that clinical symptoms and traditional Chinese medicine (TCM) syndromes of quantizing integral were improved after treatment in both groups. And the therapeutic effect of treatment group was significantly better than the control group (P< 0.01). Thesium Chinese tablets can effectively shorten the time of clinical symptom improvement time, obviously improve the level of Ig among elderly patients with acute exacerbation of chronic bron-chitis, and enhance the immune function. It was concluded that the Thesium Chinese tablets can effectively improve the quality of life among patients with chronic bronchitis (syndrome of phlegm heat in the lung).

Adult ; Aged ; Antineoplastic Agents ; therapeutic use ; Benzamides ; Chemotherapy, Adjuvant ; Female ; Follow-Up Studies ; Gastrointestinal Neoplasms ; drug therapy ; surgery ; Gastrointestinal Stromal Tumors ; drug therapy ; secondary ; surgery ; Humans ; Imatinib Mesylate ; Liver Neoplasms ; secondary ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Piperazines ; therapeutic use ; Pyrimidines ; therapeutic use

Current cancer therapies, including chemotherapies and radiotherapies, can induce toxic side effects to normal tissues with low specificity to cancer cells and usually have limited efficacy as results of drug resistance. Phototherapies kill cancer cells under irradiation of light, preferably near-infrared light with high tissue penetration, and would normally show little toxic effect in the dark. Over the past decade, nanomaterial-based phototherapies have made tremendous progress worldwide. These therapies show remarkable results against cancer in many preclinical studies. This study reviews the recent achievements in the use of functional nanomaterials for photothermal therapy, photodynamic therapy, and photo-controlled therapeutic platforms and discusses future prospects and challenges in this field.

Objective:To evaluate the value of serum anti citrullinated antibody ( anti CCP-Ab) ,anti keratin antibody ( AKA) and rheumatoid factor ( RF) diagnostic significance of these 3 kinds of index in RA.Methods:256 patients with RA ,92 patients with other autoimmune diseases,50 cases of healthy people were anti CCP-Ab,AKA,RF detection,and the results were analyzed.Results:single target detection ,diagnostic sensitivity of RA from high to low was RF ,anti CCP-Ab,AKA,specific from high to low was AKA ,anti CCP-Ab, RF,anti CCP-Ab and AKA combined detection in clinical diagnostic efficiency.Conclusion: RF,AKA,anti CCP-Ab three index have advantages and disadvantages in diagnosis of RA ,the diagnosis of AKA ,anti CCP-Ab serum of RF negative RA is a powerful complement to,in accordance with the selection of project portfolio appropriate experimental conditions ,the early diagnosis of RA and early treatment ,improve the prognosis had higher value.

Objective:To establish the quality control for Yangxueyin oral liquids. Methods:TLC was applied to identify Angeli-cae Sinensis Radix and Fructus Ziziphi Jujubae. The content of astragaloside A in Yangxueyin oral liquids was determined by HPLC-ELSD. A Luna C18(250 mm ×4.6 mm,5 μm)column was used, the mobile phase was acetonitrile-water (38∶62)with the flow rate of 0. 8 ml·min-1 , and the column temperature was 40℃. The temperature of the drift tube was 85℃, and the flow rate of the carrier air was 2.0 L·min-1. Results: The linear range of astragaloside A was 0.522-4.176 μg(r =0.999 8). The average recovery was 97. 9% with RSD of 1. 03% (n=6). Conclusion:The method is convenient, sensitive and accurate, which can be used in the quality control of Yangxueyin oral liquids.

Objective To investigate the treatment options of clomiphene(CC) resistant polycystic ovary syndrome (PCOS) related infertility. Methods Figty nine PCOS patients with CC resistant anovulation were accepted and treated by the following three protcols :(1)follicle stimulating hormone (FSH) group, 49 cycles; (2)FSH +pulsatile gonadotropin releasing hormone(GnRH) group, 13 cycles; (3)conventional in vitro fertilization (IVF)_embryo transfer(ET) group, 19 cycles. Suppressive treatment on serum luteinising hormone (LH)and testosterone (T) levels was given in the first and second groups in advance. Serum estradiol level; pregnancy rate; miscarriage rate, ovarian hyperstimulation syndrome (OHSS) and multiple pregnancy rate were compared among the three groups. Results The pregnancy rate in FSH group, FSH+GnRH group and IVF ET group werte 37%,54% and 22% respectively. The highest rate and multiple pregnancy rate was found in IVF ET group. Conclusion The rationale treatment options for CC resistant PCOS related infertility was the addition pre suppressive treatment, low dose FSH stimulated regimen subsequent pulsatile GnRH infusion. IVF were only accepted after failure with gonadotropin therapy.

Objectives:To study the mechanical properties of human dentin obtained from vital and pulpless teeth. Methods:Dentin specimens were obtained from 20 freshly extracted normal vital human teeth and 9 endodontically treated human pulpless teeth. The dentin was grinded to be rectangular specimens along to the longitudinal axes of the teeth. All the specimens were immerged in 9 g/L sodium chloride solution, their compressive mechanical properties were measured with displacement measurement method. All data obtained were analyzed with t tests. Results:The elastic moduli,proportional limits and compressive strengths of the dentin samples from vital teeth were (15.77? 1.52 ) GPa, (157.07?13.79) MPa and (257.67?10.23) MPa respectively. Those from pulpless teeth were (12.08?1.71) GPa, (144.30?9.76) MPa and (235.26?9.12) MPa respectively(P

BACKGROUND:The current studies concerning the effect of obesity on children are limited to metabolic physiology, and there is little evidence available on skeletal muscle and motor ability.
OBJECTIVE:To compare isokinetic knee muscle strength between obese children and normal children, and analyze the causes of physical performance decline in obese children.
METHODS:A total of 56 children were recruited in this study, including 28 obese children and 28 normal children. The isokinetic muscle strength was tested by CON-TREX. The gaits were tested by VICON. The statistical analysis of al the differences between obese children and normal children were measured using SPSS 19.0 software.
RESULTS AND CONCLUSION:At the same gait speed, obese children had higher absolute peak torque and average power than normal children (P<0.05), but relative peak torque and relative average power showed no significant difference compared with normal children (P>0.05) except extensor group at 60(°)/s was significantly lower than normal children. At the same gait speed, the torque and power of extensor group were significantly higher than that of flexor group in the two groups (P<0.05);he obese children showed significant differences in the absolute peak torque and absolute average power compared with relative peak torque and relative average power of flexor group at 120(°)/s (P<0.05). In the two groups, the extensor exhibited higher torque in high speed, while the high flexor torque was found in low speed. Normal children had faster cadence and walking speed, significantly smal er step width and shorter stride time than obese children (P<0.05). Obese children have smal er relative strength and higher absolute strength than normal children;in addition, the low cadence and walking speed are found in obese children. These factors contribute to weak limb strength and insufficient motor ability.