Objective To investigate the therapeutic effect of transplantation of limbal epithelial cells and decellularized porcine cornea construct in rabbits with corneal alkali burn.Methods Allogeneic rabbit limbal epithelial cells were cultured and identified.Porcine cornea scaffold was fabricated by decellularization.The LECs-DPC construct was cultured and transplanted to the alkali-burned corneal surface of rabbits(4month follow-up).Results Immunocytochemistry and RT-PCR analyses revealed the presence of ABCG2 positive limbal stem cells.A general view showed the corneas were clear and vessel-free postoperatively.HE and Masson Trichrome staining results indicated the epithelial stratification,fewer cellular infiltration,DPC' integration into host corneal tissues,and more regular collagen configuration.Transmission electron microscopy exhibited formation of desmosomes and microvilli.Conclusion Allogeneic limbal epithelial cells transplantation using DPC as a carrier can restore corneal clarity after alkali burn in a rabbit model.

Objective:To investigate the clinical effects of transcranial ultrasound to arterial recanalization in acute ischemic stroke patients.Methods: Eighty cases of patients with acute ischemic stroke from February 2010 to May 2013 in our hospital were chosen as the experimental objects, and were divided into experimental group and control group randomly. The control group were treated with urokinase and transcranial ultrasound, the experimental group were treated with urokinase and placebo. The clinical effects of each group were compared.Results: The experimental group in the 2h and 24h when the recanalization rate was obviously higher than the control group, and the difference is statistically significant(x2=8.916,x2=8.155,P<0.05). Symptoms improved rate of patients in the 2h and 24h in the experimental group was obviously higher than that of the control group, and the difference was statistically significant. The experimental group prognostic good rate was 56.09%, while the control group was 23.08%, the difference was statistically significant(x2=9.081,P<0.05). The two groups had no statistically significant difference in mortality(x2=0.005,P<0.05). Conclusion: Patients with acute ischemic stroke with transcranial ultrasound treatment can significantly improve the prognosis of the patients condition, improve the rate of recanalization.

Choroidal thickness and its relevance with retinal disease has been widely studied in recent years,as choroid is an important source of retina blood supply.Diabetic retinopathy (DR) studies have been focused on retinal vascular injury and related circulatory disorders for a long time,however recent studies have found that choroidal blood vessels are also affected by diabetes,including the thickness changes.The choroidal thickness of diabetic patients is thinner than normal.There is no conclusive conclusion about choroidal thickness and the severity of DR lesions,as the choroid thickness results are determined by multiple factors,including diseases,ocular conditions (choroidal vascular status,ocular axial length,refractory errors and other eye parameters),systemic factors (age and sex) and measurement methods.Therefore,it is necessary to calibrate the ocular and extra-ocular factors affecting choroidal thickness when trying to further clarify the relationship between the choroidal thickness and DR.

Objective To evaluate the value of ultrasonic elastography for diagnosis of chronic nonspecific low back pain. Methods From March to September, 2016, 32 patients diagnosed as chronic nonspecific low back pain and other 32 healthy people (controls) were measured lumbar erector spinae with ultrasonic elastography, and calculated Yang's modulus. The correlation between Yang's modulus and Visual Analogue Scale (VAS) or Japanese Orthopaedic Association (JOA) score in the patients were investigated with Pearson's analysis. Re-sults There was no significant difference in Yang's modulus between bilateral lumbar erector spinae in both the patients and the controls (t<1.849, P>0.05), but it was more in the patients than in the controls (t=9.931, P<0.001). The Yang's modulus was positively correlated with VAS score in the patients (r=0.614, P<0.001), but not with the JOA score (r=-0.243, P=0.180). Conclusion Ultrasonic elastography can be applied to differentiate low back pain from the healthy, and measure the intensity of pain.

Objective To observe the efficacy and safety of arginine vasopressin receptor antagonist tolvaptan for treating hyponatremia caused by syndrome of inappropriate secretion of antidiuretic hormone (SIADH).Methods Six patients diagnosed with SIADH were enrolled in this study.Four cases were allocated to tolvaptan group (oral tolvaptan 15-60 mg/d,the dosage was adjusted based on the level of serum sodium).Two cases were allocated to regular treatment group (liquid was limited 1 000 ml/d,intravenous drip was less than 3％ sodium chloride and/or oral salt capsule 10-15 g/d several times was adjusted based on the level of serum sodium).Data of the level of serum sodium at 4,7 d,baseline value of serum sodium,serum sodium at the first normal time,24 h urine and weight change were collected.Safety assessment was given before and after treatment,including medical history,physical examination,electrocardiogram,laboratory tests,and incidence of adverse events.Results The level of serum sodium in tolvaptan group increased from the first day of the treatment.During the period of treatment,serum sodium at 4 d increased 22,16,14,11 mmol/L compared with the baseline value respectively.No obvious change of sodium was observed in regular treatment group compared with the baseline value.For 7 d treatment,serum sodium level increased 14,13,14,13 mmol/L in tolvaptan group compared with the baseline level respectively.Only 2 cases in regular treatment group increased 4 mmol/L,and sodium level in regular treatment group did not reach the lower limit of normal level of serum sodium.In tolvaptan group,all patients urine output increased after 1 d treatment and began to stabilize in 3 d.24 h urine putouts were much more than 1 500 ml during treatment.Urine putouts 780-1 400 ml were observed in regular treatment group.There was no difference before and after treatment.Although weight dropped after treatment in the two groups,weight in tolvaptan group fell obviously.In the two groups,there was no difference in blood pressure and heart rate before and after treatment.There were no serious complications and adverse events.Conclusions Compare with regular treatment,arginine vasopressin receptor antagonist is more effective therapy because it can correct the SIADH in patients with hyponatremia and reduce water retention.It also has a good security.

Objective To further identify the isolated Mycoplasma genitalium (Mg) from the high risk population of STD in China. Mothods The morphology of Mg standard strains and clinical isolates were observed under electron microscopy (EM) and atomic force microscopy (AFM). Mg sample preparation for AFM was fixed on mica and AFM images of scanning observation were captured by contacting and tapping modes and operated under normal atmospheric pressure and temperature. Results and Conclusion Mg had a diversity of shapes, including flask, pear, spindle or round shape with projecting neck portion and slightly broadened terminal structure under EM. The morphologic features under AFM were similar to those observed by EM. The typical Mg showed shapes of flask and pear with noticeable narrow ring or mark in the neck portion. The size of Mg measured under EM and AFM was similar as well. Mycoplasma

Objective To study the curative effect and mechanism of moxibustion on heat-sensitive acupoints combined with Erwei Badu Powder in the treatment of elderly herpes zoster (HZ) with type of stagnant heat of liver channel. Methods Sixty-five patients with HZ were randomly divided into treatment group (35 cases) and control group (30 cases). Two groups were orally given Longdan Xiegan Decoction and aciclovir conventionally, the treatment group was given moxibustion additionally on heat-sensitive acupoints combined with Erwei Badu Powder, and the control group was only given external application of Erwei Badu Powder for 10 days. The symptom scores, levels of T lymphocyte subsets and serum interleukin-2 (IL-2) were observed before and after treatment, the curative effect was evaluated, and PHN was followed up. Results The effective rate was 85.71%(30/35) in the treatment group and 66.67%(20/30) in the control group. The curative effect of treatment group surpassed the control group (P<0.05). Incidence of PHN was lower (P<0.01). The levels of CD4, CD4/CD8, and IL-2 increased and the level of CD8 decreased significantly in two groups compared with those before treatment (P<0.05). All indexes were better in the treatment group than those in the control group (P<0.05). Conclusion The curative effect of moxibustion on heat-sensitive acupoints combined with Erwei Badu Powder in treatment of elderly HZ is better than that of Erwei Badu Powder only, particularly in reducing the occurrence of PHN, improving neuralgia and insomnia. The mechanism may be related to increasing levels of CD4, CD4/CD8 and IL-2, and improvement of body’s cell-mediated immunity.

Objective To assess the feasibility of ~(18)F-FDG PET/CT for differentiation of malignancy from benign in pericardial Lesions. Methods ~(18)F-FDG PET/CT data of 16 patients with pericardial lesions (9 malignancy, 7 benign) were analyzed using maximum standard uptake values (SUV_(max)) and the density of lesions, the relationship with surrounding tissues were also evaluated. The results of malignancy or benign were proved by needle biopsy.Results SUV_(max) of malignancy (10.1±6.0) and benign (3.0±2.1) was significant different (P<0.01). Taking the optimal cut-off value as SUV_(max) 3.5-4.5, the sensitivity, specificity, accuracy, positive predictive value and negative predictive value of differentiation of malignant from benign in pericardial lesions was 100%, 85.71%, 93.75%, 90.00% and 100%, respectively. Conclusion ~(18)F-FDG PET/CT can predict benign or malignance of pericardial lesions with moderate accuracy.

Objective To study the different curative effect between r-tPA application and urokinase in the interventional therapy for acute and severe pulmonary embolism.Methods Analyzed the clinical date of 22 cases acute and severe pulmonary embolism patients,and classified them into two groups according to the application of r-tPA and urokinase to compare the changes of their blood pressure,heart rate,arterial partial pressure of oxygen,hemachrome and pulmonary arterial pressure before and after therapy,meanwhile,record the thrombolysis time that the two groups took.Results The thrombolysis effect time adopting urokinase was remarkably longer than that thrombolysis adopting r-tPA(P<0.05); The blood pressure,heart rate,arterial partial pressure of oxygen of the two groups gained remarkable improvement after therapy(P<0.05).The pulmonary arterial pressure of the urokinase group dropped obviously after operation(P<0.05),while the r-tPA group dropped not so significantly after operation.The hemachrome of the two groups did not dropped significantly after operation.Conclusion The r-tPA and urokinase are both effective to interventional therapy for acute and severe pulmonary embolism,while r-tPA can clearly shorten the time in thrombolysis and could reduce any bleeding risk.

Objective To investigate the effect of HP1γ on the development of ovarian cancer. Methods RT-qPCR was used to deteched the level of HP1γin ovarian cancer samples. The proliferation of A2780 and SKOV3 cell line was detected by CCK8 assay. RNAi was used to knock down the expression of HP1γ mRNA. Results The level of HP1γwas higher in ovarian cancer than that in the normal tissue. Mir-30b may inhibit the proliferation of ovarian cancer cell. Conclusion HP1γpromotes the proliferation and progress of ovarian cancer cells.