This paper describes briefly the newest progress in the early diagnosis and prediction of the osteonecrosis of the femoral head (ONFH) after femoral head fracture. It is necessary that early operative intervention and aspiration of the intracapsular blood should be done in order to minimize the occurrence of ONFH. It is wise to avoid positioning the hip in extension and internal rotation until surgery. Traction with hip in flexion is recommended. Using the contrast enhanced (Gd DTPA) MRI with T1 fat saturation imaging within 24 hours after injury, the prediction of ONFH will be possible. It is recommended the ONFH should be divided into two types, namely silent and clinical ones.

BACKGROUND:Decel ularized scaffolds are special for retaining the tubular structure used for nutrition transport, and providing a similar inner environment for cel growth. OBJECTIVE:To study the preparation of the decel ularized whole liver bioscaffold and to explore its repair outcomes for liver injury. METHODS:Livers from 12 Sprague-Dawley rats were used for preparing the decel ularized whole liver bioscaffold by chemical detergent-enzymes decel ularized technology. Models of liver injury were established in another 24 Sprague-Dawley rats and randomized into two groups:the decel ularized whole liver bioscaffold was implanted into the rat liver lesions in experimental group, and controls were given the injection of normal saline. Thirty days later, the serum levels of alanine aminotransferase and glutamic-oxaloacetic transaminase were detected, and liver tissues were removed for hematoxylin-eosin staining. RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that extracel ular matrix-like structures existed in the decel ularized bioscaffold;cel components were completely removed from the liver, the col agen fibers in the scaffold arranged regularly and were not dissolved under electron microscope. The serum levels of alanine aminotransferase and glutamic-oxaloacetic transaminase in the experimental group were significantly lower than those in the control group (P<0.05). Hematoxylin-eosin staining showed a large number of blue-stained and dense distributed nuclei, and pink distribution of col agen fibers that had no overt breakages in the control group, while pink and dense structures in the experimental group. These results suggest that the decel ularized whole liver bioscaffold is easy to obtain, and can promote the injured liver repair.

To sum up the methods which can be used to prevent dislocation after total hip replacement.Recent original articles about the reasons of dislocation and methods which were used to treat or prevent dislocation were extensively reviewed,and retrospective and comprehensive analysis was performed.Dislocation after THA can be reduced by careful assessment of patients,selecting suitable prosthesis,improving the position of prosthesis and avoidance risk movements.Dislocation after THA can be reduced by careful pre-,intra-,and postoperative assessment.

Objective To investigate the natural history of mild adolescent idiopathic scoliosis in a period of 15 years and search for the risk factors of progress as well as to recommend the modification program of school screening for scoliosis. Methods From a group of 204 patients with adolescent idiopathic scoliosis who were detected from school screening for scoliosis among 20 418 school children in Beijing from May 1985 to January 1986, 90 of the patients were reevaluated 15 years later. The follow-up items of the therapeutic history, physical examination and standing spinal X-ray films were carried out. The late data were compared statistically with the original data at the time of school screening. The final results were divided into four groups: 1)Resolved group with a current curve less than 10 degrees; 2)Reduced group with a current curve more than 10 degrees but reducing less than 5 degrees regarding to the original status; 3)Unchanged group with a current curve more than 10 degrees while progressing less than 5 degrees compared to the original data; 4)Progressed group with a current curve was more than 10 degrees and aggravating more than 5 degrees than the original curve. Results Of 204 patients, 111 patients were followed up, and X-ray films were taken in 90. The follow-up results suggested that 29 cases showed resolved(32.2%), 21 cases reduced (23.3%), 30 cases unchanged (33.3%) and 10 cases progressed (11.1%). In 10 cases of progressed group, there were 8 females and 2 males, furthermore, 6 patients were found with the cobbs angle of curve between 10 and 19 degrees and 4 patient with the cobbs angle of curve more than 20 degrees when school screening. The curve pattern of the 10 progressed patients consisted of double thoracic curves in 2, double thoracic and lumbar curves in 2, right thoracic curve in 3, thoraco-lumbar curve in 2 and lumbar curve in 1. Conclusion Most of cases with less than 20 degrees curve are of no progress. The high risks of progress are as followed: curve more than 20 degrees, female, double thoracic curve, double thoracolumbar curve, right thoracic scoliosis and top vertebral rotation more than grade Ⅱ. The school screening for scoliosis should be carried out by school medical team who were trained by orthopaedists. The specialists should reexamine the suspected children and monitor the patients with high risk of progress.

BACKGROUND: As the result of estrogen shortage due to ovariectomy,osteoporosis occursin the general and local bones, displaying bone loss and changes in bone microstructure and growth factor mRNA expression,which definitely has an important effect on fracture healing. OBJECTIVE: To probe into the effect of estrogen on bone microstructure and bone morphogenetic protein-4 mRNA expression and location during fracture healing. DESIGN: Randomized controlled study. SETTING:Laboratory of the Department of Molecular Biology of Yunnan University. MATERIALS: This study was conducted at the laboratory of Molecular Biology Department of Yunnan University between July 1999 and July 2002. We recruited 96 healthy female SD rats of 2 months old and with the mean body mass of 160 to 200 g. METHODS:①Of the 96 rats that received intraperitoneal anesthesia, 48rats were subjected to bilateral ovariectomy(osteoporotic group), and the other 48 rats received sham operation (control group). One month later, bilateral tibia fracture at the middle segment was artificially made on all rats under anesthesia, and no treatment was given so as to prepare fracture healing model. Then rats of both groups were put to death for collecting callus and the surrounding parenchyma at postoperative 1, 3, 5, 7, 14, 28,56 and 112 days, with 6 rats in each time point. ② The tibia bone microstructure was observed under electron microscope. ③ The mRNA expression of bone morphogenetic protein-4 at callus and the surrounding paranchyma was detected by RT-PCR method; no probe in hybrid fluid was used as negative controls. Six in situ hybridization slices with positive expression were selected from both groups at postoperative 1 and 3 days time points, and 3 visual fields were randomly selected from each slice for observing the positive granules under 25 times field lens. MAIN OUTCOME MEASURES: ①The tibia bone microstructure;② bone morphogenetic protein-4 mRNA expression at callus and the surrounding parenchyma. RESULTS: All the 96 rats entered the final results analysis. ① Observation of the tibia bone microstructure: at 28 days after tibia fracture, osseous callus and ostein fibers were found arranged densely in control group. Osteocytes, small with fewer cytoplasts, were observed in osseous lacuna, but osteoclasts were found surrounding small-sized bone trabecula. In osteoporotic group, fibrous callus and collagenous fibers in bone matrix were arranged loosely, lots of big osteoblasts could be observed with osteocytes easily seen. ② Bone morphogenetic protein-4 mRNA expression at callus and the surrounding parenchyma: it was less expressed in control group than in osteoporotic group at postoperative 1 to 3 days (23.714 3±5.056 8,21.714 3±5.023 8 vs 51.285 7±8.138 7,49.571 4±9.071 1, P ＜ 0.01) and the expression was mainly observed in parenchyma surrounding the fracture where callus was formed. CONCLUSION: Bone morphogenetic protein-4 mRNA expression is increased in osteoporotic group and is mainly observed in parenchyma surrounding the fracture, displaying a manner of regional expression. However,the formation and quality of callus matrix during fracture are obviously poorer than in control group.

Objective To explore the selection of prostheses for the elderly patients with severe displaced fracture of the femoral neck through retrospective comparison of the long term clinical results between bipolar hemiarthroplasty and total hip replacement. Methods From January 1986 to December 2002, 101 cases of Garden Ⅲand Ⅳtypes of the femoral neck fracture were treated with either bipolar hemiarthroplasty (Group A: 55 cases) or total hip replacement (Group B: 46 cases). The age of the patients at the time of operation ranged from 55 to 94 years old (mean: 70.4 years old) in Group A, and from 57 to 75 years old (mean 69.5 years old) in Group B. The average period of follow up was 4.9 years (1 to 10 years) in Group A and 3.5 years (1.5 to 5 years) in Group B. The clinical results were evaluated according to Harris and Merle d'Aubigue criteria. The perioperative complications were compared. Results The excellent and good clinical results were 78.12 %in Groups A and 95.65 %in Group B respectively. The rate of groin and thigh pains was higher in Group A (36.36 %) than in Group B (6.52 %). The flexion of the hip in Group A was more limited (mean 85 degrees) than in Group B (mean 110 degrees). There were more cases of sunken femoral stem in Group A. The perioperative complications were similar in both groups. Conclusions The total hip arthroplasty is the first choice for the elderly patients with severe displaced femoral neck fracture if the conditions of the patient and the skills of the surgeon permit. The bipolar hemiarthroplasty is suitable for patients of more than 75 years old, or for those with severe diseases.

Objective To explore the indication, to describe the key of operative technique in detail and to summarize the early results in mini-incision and minimally invasive surgery (MIS) total hip arthroplasty (THA) for hip disorders. Methods From Mar. 2003 to Mar. 2005, 43 patients (49 hips) were performed with MIS THA. The mean age of patients was 53.2 years ranging from 22 to 79 years. There were 22 males and 21 females. The average body mass index (BMI) was 23.4 ranging from 17.1 to 30.2. Using the modified postero-lateral approach, MIS THA was performed with cementless prostheses. For comparison, 35 patients (43 hips) were performed with standard THA at the same period. The age and BMI of patients in both groups were similar, but the pattern of disorders was different. The comparative items included preoperative bleeding, operative time, incisional length, postoperative functional recovery, and prosthetic position. Results 31 patients (MIS THA) and 25 patients (standard THA) were followed from 6 to 24 months (mean 13.1 months). No complications occurred in MIS THA. Dislocation appeared in one patient with standard THA at the two days after operation. The length of incision was 9.3 cm (range, 8.7 to 10.5 cm) in MIS group and 16.8 cm (range, 14 to 20 cm) in standard THA group. The difference was significant statistically (P

Objective To explore the value of the percentage of necrotic surface area in predicting collapse of osteonecrosis. Methods 15 hips of avascular necrosis of the femoral head(ANFH) in 9 patients underwent MRI scan. On the MRI films, the percentage of necrotic surface area were calculated. While on the specimens of the 15 hips, the percentage of necrotic surface area were also measured. In 16 hips of 8 patients of ANFH with serials MRI at mean 18.9 months intervals (range, 6-41 months), the percentage of necrotic surface area were calculated and compared. In 38 hips of 27 patients without collapse, the percentage of necrotic surface area and the index of necrosis were calculated on MRI films. Follow-up was terminated when crescentic sign illustrated on X-ray film or CT demonstrated articular facet collapse. Those hips without collapse were followed up more than 24 months. The value of the percentag of necrotic surface area and the index of necrosis in predicting collapse of osteonecrosis was observed. Results The percentage of necrotic surface area were 63.23%?10.16% on specimens and 63.60%?7.78% on MRI films respectively. There were no significant differences between them. The percentage of necrotic surface area on specimens were coincidence with those on MRI. In the 16 hips with serials MRI; the percentage of necrotic surface area were 52.37%?19.91% on the first MRI films, 51.70%?21.29% on the second. There were no significant differences between them. This result revealed that the percentage of necrotic surface area did not vary with the extension of course of disease. In the 38 cases of early necrosis, 28(73.7%) hips collapsed and 10(26.3%) hips did not. The relative risk of the percentage of necrotic surface area was 1.043, and the relative risk of the index of necrosis was 1.020. No significant difference was found in ARCO staging for patients with or without femoral head collapse. It demonstrated that whether ischemic necrotic femoral head collapse or not had no relation with its ARCO stage. Conclusion The percentage of necrotic surface area can be used as more accurate predictor for future collapse.

[Objective]To explore the correlation between anticardiolipin antibodies and post-SARS patients with osteonecrosis,investigate the etiology of post-SARS osteonecrosis,and select the sensitive molecular symbols for early diagnosis and distinguish the high risk population.[Method]The studied subjects were divided into two groups.Sixty-two post-SARS patients with osteonecrosis was one group,and 52 matched healthy people was as normal controlled group.Empty stomach blood samples from cubital veins were collected from both groups.IgA,IgG and IgM of anticardiolipin antibodies types were examined by ELISA.[Result]Twenty-one of 62 post-SARS osteonecrosis patients were tested at least one type of anticardiolipin antibodies positive,among them,with IgA(11.33?11.209)APL,IgG(5.127?5.927)GPL,IGM(17.821?10.606)MPL.Four of 52 control group persons were detected positive,with IGA(10.702?3.126)APL,IgG(5.184?4.780)GPL,IgM(14.684?5.516)MPL.There were significant differences between two groups,while,there were no difference in prothrombin time(PT),thrombin time(TT),prothrombin activity(PTA)and international normalized ratio(INR).[Conclusion]The study indicates an increased positive incidence of anticardiolipin antibodies in post-SARS patients with osteonecrosis.This has reflected that anticardiolipin antibodies may play a role in osteonecrosis.

Objective To determine the applicability of locally continuo u s application of alendronate for the prevention of prosthesis aseptic loosening. Methods Using the method of in vitro cell culture to detect the influence of a lendronate with different concentrations(1?10－11mol/L,1?10－9mol/L,1?10－7mo l/L,1?10－6mol/L,1?10－5mol/L) on the proliferation, secretion and osteogenesi s of human osteoblasts as well as the effect of the motility, respiration, phago cytosis and bactericidal ability. Results Higher concentration of alendronate(1 ?10－6mol/L,1?10－5mol/L) improved local antiinfection ability, but inhibited osteogenesis (1?10－5mol/L). Lower concentration(1?10－11mol/L,1?10－9mol/L,1 ?10－7mol/L) inhibited the bactericidal effect of neutrophils, but stimulated o steogenesis(1?10－11mol/L,1?10－9mol/L). Conclusion Locally continuously usin g alendronate around prosthesis to prevent and treat aseptic loosening must be h andled with discretion. Its concentration should not be below 1?10－6mol/L in the early period after arthroplasty operation, but the concentration should be 1?10－11mol/L or 1?10－9mol/L in the late period.