The animal experiments,clinical researches and epidemiological investigations indicated that genistein has the effect for prevention and treatment of osteoporosis,cardiovascular disease and cancer.Genistein can inhibit cellular and humoral immunity and can regulate cell apoptosis.The chemical structure,biological effects and the mechanism of genistein attracted much attention.The current research advance on genistein was summarized and the prospect on the theoretical significance,clinical importance was presented in this paper.

Hypothyroidism is one of the most common endocrine disorders in childbearing or pregnant women.Gestational hypothyroidism can increase the incidence rate of the variety of pregnancy complications and adverse pregnancy outcome.It also can make adverse effects on the fetus and neonate,such as death,placental abruption,fetal malformation,intrauterine growth retardation and preterm,low birth weight,neonatal respiratory distress syndrome,it can also cause abnormal thyroid function of neonate,severely can cause nerve,mental,physical and mental developmental abnormalities and even death.Therefore,it is recommended that childbearing women who are at high risk for pregnancy should strengthen to monitor pregnancy thyroid function and treat as soon as possible in order to reduce the adverse effects on offspring.

OBJECTIVE: To evaluate the current situation and trend of narcotic drug use in our hospital where the authors work in order to provide the reference for scientific management and rational use of these drugs. METHODS: The yearly amount of narcotic drugs administered in the whole hospital, the yearly amount in the separate departments, as well as drug expenditures and ratios between January 2002 and November 2005 were added up and analyzed statistically. RESULTS: The amount of bucinnazine use dominated in the first. The amount of morphine for oral use was increasing year by year. The amount of fentangl transdernal patch use was also bigger and increasing rapidly. However, the amount of pethidine and morphine for injection use was decreasing. CONCLUSION:Analgetics for oral and transdermal use will be the main categories of analgetics in the future.

Under active exploration and practice of several generations of organ transpant workers,organ transplantation in China has developed rapidly since 2000 and achieved remarkble results.Techniques of transplantation become mature,and liver transplantation and renal transplantation have reached international advanced level,which benefit massive patients.With high attention and promotion of national government and health administrative departments,structural changes have occurred to source of national organ transplantation to form a China Model.The donation after citizen's death has become the new normal in the organ transplantation in China.Human organ donation work promots the rapid development of transplantation in China,but it also faces challenges of the donor organ shortage and quality improvement.Under the new situation,health,family planning and medical reform work of the national13th Five Year Plan puts forward new requirements on human organ donation and transplantation.Combined with practical work,this article gives a few opinions on how to scientifically further improve the quality and quantity of organ donation and transplantation and directions of future works.

Objective To investigate the changes of atherosclerosis and vascular tissue after pecutaneous tranluminal angioplasty (PTA) ,and evaluate the role of serum lipid ,lipoprotein (a) [Lp(a)] and tumor necrosis factor‐α(TNF‐α) in this progress .Methods Thirty New Zealand white rabbits with high fate diets were established as observation group ,and 10 rabbits with normal feed were set as control group .Blood biochemistry test was used to detect the levels of serum TC ,TG ,HDL‐C ,LDL‐C ,Lp(a) and TNF‐αbefore the experiment ,after 2 weeks feeding and 1 month ,2 months ,3months after PTA .The correlation among atherosclerosis and Lp(a) ,TNF‐a were measured by multiple logistic regression analysis .Opposite reference value was calculated with receiving operating characteristics (ROC) analysis .Results The levels of TC ,TG and LDL‐C were significant higher than baseline with the feeding time(P<0 .05) .The level of HDL‐C after 2 weeks high cholesterol diet feeding was lower than baseline (P<0 .05) .The content of TNF‐αat 2nd month after PTA was remarkable higher than baseline(P<0 .01) .ROC analysis showed that RR of TNF‐a was 1 .84(P<0 .01) and an area under the curve of 0 .871 for TNF‐α (95% CI:0 .811 -0 .956) compared to 0 .523 for Lp(a) (95% CI:0 .409-0 .720)regarding atherosclerosis .Conclusion The turbulence of lipid could be an important factor of atherosclero‐sis ,and the high level of TNF‐αmay take part in the process of repairing of arteriosclerotic vessel .

Objective To investigate the blood specimen collection site,inspection time and hemolysis influ-ence on biochemical test results.Methods The specimens of 2586 cases outpatients and hospitalized patients in our hospital who accepted blood routine and blood coagulation four check were analyzed.The hemolysis and qualified specimens of biochemical test results were compared.Results 2 586 specimens found unqualified blood samples in 18 cases,and the incidence rate was 0.70% (18 /2 586).Samples of different section and source were compared and found there were no significant difference (F =0.36,P >0.05).In 18 cases of unqualified blood samples due to technical problems and unqualified sample accounted for 88.89% (16 /18).The technical factors that lead to unqualified samples was significantly higher than that of the instrument factor or fixed factors,and the difference was statistically significant(χ2 =152.63,98.52,all P <0.05).In addition,hemolysis caused the most of the unqualified samples for the technical factors.To choose potassium infusion on the side ipsilateral to the site or from the opposite side of the site collected blood samples.Sodium,chlorine,creatinine,uric acid,glucose detection found in effect of blood on the same side of the infusion of the biochemical test results in varying degrees.Potassium,chlorine,sodium, creatinine decreased significantly,and uric acid,glucose and other indicators were significantly increased (t =4.51, 4.98,5.50,3.25,10.18,15.25,all P <0.05).Gamma transpeptidase,creatine kinase,sodium and uric acid and other index of hemolytic samples were significantly lower than those of normal samples,and alanine aminotransferase (ALT),direct bilirubin,potassium,phosphorus,serum creatinine and creatine kinase were significantly higher than those of the normal samples (t =14.85,21.21,7.15,4.86,10.33,4.02,3.11,8.20,7.54,5.11,all P <0.05).This may cause the depth in erythrocytes and some related substances.The time of collecting blood to sent to the room had obvious influence on the biochemical test results,alanine aminotransferase,aspartate amino transferase,glucose, creatine kinase and hydroxybutyrate and project them 1 h after submission were significantly lower than those of the standard inspection,and 1 hour after the submission of lactate dehydrogenase and creatine kinase isoenzyme index were significantly higher than those of the standardize inspection means (t =2.95,4.82,2.88,5.44,4.05,3.98, 4.89,all P <0.05).Conclusion Sampling sites,hemolysis and inspection time had effect on blood specimen,so for the collection of blood samples must be strictly in accordance with the requirements of the operation.

Objective:To investigate the use of antibacterial drugs during the perioperative period of a cesarean section and observe the differences before and after antibacterial drug remediation. Methods:1 569 cesarean cases from 2010 to 2012 were investigated, and the use of antibacterial drugs, drug categories, drug combinations, medical expenses, and so on was statistically analyzed with the use of the statistical software SPSS13. 0. Results:The rate of antibacterial drug usage within the 1 569 cesarean cases was 100%;for pre-operative antibacterial drug remediation, azithromycin, cefathiamidine, and ornidazole were the top three, while the top three antibacterial drugs for post-oper-ative remediation were arecefathiamidine, cefuroxime, and clindamycin. There was no significant difference between the two groups of incision infections, and the rate for combined use of drugs dropped. The analysis of drug costs is of statistical significance. Conclusion:The analysis of antibacterial drug remediation is useful for the rational use of anti-bacterial drugs and a reduction of costs.

Antibiotic resistance is a serious global public health problem, and China is one of the countries with the serious problem of irrational antibiotic use and bacterial drug resistance.The world is headed for a post-antibiotic era.With the rapid development of microecology, microbiota has been linked with antibiotic resistance.From microecological point of view, it is possible for researchers in different fields to innovate new techniques for bacterial drug resistance, and to explore the evolution of pathogenic bacteria as well as the mechanism of their resistance to antibacterial agents.This paper reviews the research progress in bacterial resistance and microecology, and prospects the future research needs that should be prioritized to tackle antibiotic resistance.

Gastrointestinal motility disorders are commonly seen digestive system diseases in elderly subjects. During the process of normal aging,digestive tract experiences a series of degenerative changes,which is the foundation of many gastrointestinal motility disorders. Further study on gastrointestinal dysmotility and its mechanisms is of great significance for the diagnosis and treatment of related diseases in aging population. In this article,the advances in studies on effects of aging on motility of digestive tract( including oropharynx,esophagus,stomach,small bowel,colon and anorectum)and its mechanism researches were reviewed.

Bedside ultrasonography is used to provide information about the cardiovascular system and the function and anatomy of certain internal organs,and help to guide the invasive procedures.Compared to traditional ultrasonography,bedside ultrasonography is more focused on the optimization of the treatment.It has become a necessary tool,as a powerful adjunct to the physical examination,in the management of critically ill patients in advanced countries.The development trend is the clinical protocol based on bedside ultrasonog-raphy.The application of bedside ultrasonography in China is limited,especially in PICU.Standardized educa-tion and quality assurance system are needed to its popularization.