AIM: To establish the content determination method of matrine and phillyrin in Jieyinjing Suppositories(Radix Sophorae Flavescentis,Fructus Forsythiae,Cortex Phellodendri Chinensis,Radix Stemonae,etc.) by HPLC. METHODS: HPLC was performed to determine matrine on ODS column,the mobile phase consisting of methanole-0.02% triethylamine(55∶45) and detection wavelength at 220 nm and the phillyrin of mobile phase consisting of acetonitrile-water(25∶75) and its detection wavelength at 277 nm. RESULTS: The linearity of matrine was in the range of 9.8 ?g?mL~(-1)-49.0 ?g?mL~(-1)(r=0.999 96),and the average recovery was(99.48%,) RSD=1.49%;The linearity of phillyrin was in the range of 5.4 ?g?mL~(-1)-54.0 ?g?mL~(-1)(r=(0.999 9),) and the average recovery was 100.07%,RSD=1.34%. CONCLUSION: This method is simple,sensitive and accurate,and available for control of Jieyinjing Suppositories.

Objective To investigate changes in serum bilirubin and internal environment,etc.of neonatal hyperbilirubinemia that before and after the treatment of peripherally arteriovenous synchronous exchange transfusion. Methods In the strict monitoring of vital signs,hyperbilirubinemia peripheral vessels exchange transfusion was applied in five cases of neonatal,and the serum bilirubin,routine blood test,electrolytes before and after the exchange transfusion were detected.Results By comparing the data before and after the exchange transfusion,the total bilirubin and indirect bilirubin decreased obviously,respectively[(414.02 ±56.04)μmol/L,(225.72 ±53.54)μmol/L,t =5.433 P =0.01,(381.82 ±44.68)μmol/L,(199.22 ±57.37 )μmol/L,t =5.615 P =0.01 ],the difference was statistically significant,and also the platelet count were decreased obviously,respectively [(199.00 ± 42.01)×109 /L,(102.00 ±17.72)×109 /L,t =4.758,P =0.001],the difference was statistically significant.After the exchange transfusion,white blood cells,hemoglobin,potassium,sodium,chloride,calcium,glucose,blood pressure, heart rate and blood PH value had decreased or increased slightly,there was no significant difference P >0.05.Cere-bral palsy and other complications did not occur after 12 months of follow -up of the five cases.Conclusion The outer peripheral arteriovenous synchronous exchange transfusion therapy for neonatal severe hyperbilirubinemia can quickly reduce serum bilirubin,and has less impact on the body environment,it is an important and effective salvage treatment of neonatal hyperbilirubinemia,thereby reducing damage to the nervous system of bilirubin.

Neurobiology is a cross subject,in which"Language and Attention"is an impor-tant chapter as a hotspot involved with physiology,psychology,anatomy and biology.Inorder to im-prove the teaching efficiency,it is necessary to apply the techniques such as arranging the content logically,giving prominence to the emphases and breaking through the difficulty,using interactive teaching method and collocating the assistant method rationally. Through this way,it is easier for the students to get interested in this chapter and grasped the content.

Objective To investigate the effect of puerarin on preeclampsia-like rat placental tissue hypoxia inducible factor (hypoxia-inducible factor,HIF)-1αexpression and study the mechanism of protective effects in rats.Methods 60 SD rats were randomly divided into control group,drugs intervention groups(low-dose,middle-dose,high dose)and model group.The rats in drugs intervention groups and model group were adopted endotoxin for induction of preeclampsia model.On the basis of the model group,low,medium and high dose groups were given 20.0,40.0,60.0 mg/kg puerarin by intraperitoneal injection,respectively.The blood pressure of tail artery,urine protein concentration were observed at different points and the expression of HIF-1αwere detected by PT-RCT in each group.Results The systolic blood pressure ,urine protein and HIF-1αprotein in model group were higher than control group(P<0.05 );serum NO concentration was lower than control group (P<0.05 );after the puerarin intervention,systolic blood pressure and urine protein concentration in drugs intervention groups were lower than model group (P<0.05 ),plasma NO concentration was higher than model group (P<0.05 )and HIF-1αprotein was lower than model group (P<0.05 );the placenta weight,weight and body length of newborn rats in drug intervention group were higher than model group (P<0.05 ).Conclusion Puerarin can reduce systolic blood pressure and urine protein levels,promote the level of NO in plasma and reduce HIF-1αlevel in placenta of rats.Puerarin can offer protection to rats with preeclampsia by decreasing the expression of HIF-1αin placental.

Objective To establish a method for the content determination of naringin in Bogu Pill by HPLC. Methods At room temperature and by using ultrasonic extraction, HPLC was performed to determine naringin content on ODS chromatographic column.The mobile phase consisted of a mixture of acetonitrile and 8 % acetic acid (15 ∶ 85)and detection wavelength was at 283 nm.Results The linearity of naringin was in the range of 5.2 ? g / mL～ 31.2 ? g / mL (r=0.9999) and the average recovery was 98.72 % , RSD=1.55 % . Conclusion This method was simple, sensitive and accurate, and can be used for the quality control of Bogu Pill.

Objective To explore the clinical effect of systemic nursing intervention on constipation after coronary heart disease. Methods 145 patients were randomly divided into intervention and control group. Patients in invention group were given systemic nursing intervention, including diet, exercise, health education and so on. The patients in control group were treated with cathartic.Results There was significant difference between intervention and control group in incidence of constipation or related complication(P<0.05). Conclusion Systemic nursing intervention is helpful to reduce constipation and prevent the related complication.

Objective To explore an optimal scheme for diagnosis and treatment of patients with acute viral myocarditis (AVM) with an experience prescription of professor Zhang Zengying, Junkang oral liquid.Methods A prospective study was conducted. Sixty patients with AVM admitted into the Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine (TCM) from March 2012 to January 2014 were randomly divided into two groups: a control group and a treatment group (each, 30 cases). The patients in control group were treated with routine western medicine, including antiviral drugs, polarized solution, adenosine disodiu, and oxygen inhalation; while in treatment group, besides the comprehensive treatment in the control group, the patients were given Junkang oral liquid (main ingredients: Codonopsis pilosula, Salvia miltiorrhiza, Polygonatum odoratum, Notopterygii, Paris polyphylla Smith var. chinensis Hera, Forsythia suspensa, rhizome Ligustici Chunxiong, Hedyotis diffusa, Ophiopogonis tuber), 10 mL each time, three times a day; the treatment was consecutively applied for 3 months in both groups, 15 days constituting one therapeutic course. The changes of cardiac function [heart ejection fraction (EF), stroke volume (SV)] and myocardial enzyme [creatine kinase (CK), MB isoenzyme of creatine kinase (CK-MB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH)], and the TCM syndrome integral score, symptoms and signs such as fever with chills, palpitation or severe palpitation, oppressed feeling in chest, deficiency of qi and disinclination to talk, dizziness, weakness and shortness of breath, spontaneous sweating, night sweating, body limb aches, premature beat shown on electrocardiogram (ECG) and curative effect were observed before and after treatment in the two groups.Results Compared with those before treatment, in the two groups after treatment, the EF and SV were significantly elevated, while the CK, CK-MB, AST, LDH, TCM syndrome integral score were obviously decreased, the degree of improvement in the treatment group being more prominent [EF: 0.601±0.088 vs. 0.538±0.084, SV (mL): 67.02±6.78 vs. 61.03±5.88, CK (U/L): 76.25±28.41 vs. 92.54±31.68, CK-MB (U/L): 18.34±8.94 vs. 22.45±9.70, AST (U/L): 31.29±14.28 vs.33.45±11.21, LDH (μmol·s-1·L-1): 2.28±0.51 vs. 2.34±0.68, TCM syndrome integral score: 12.34±1.24 vs. 18.36±1.98, allP < 0.05]; the degrees of improvement in symptoms and signs such as palpitation or severe palpitation, oppressed feeling in chest, shortness of breath, exhaustion, etc and the total effective rate of premature beat in treatment group were significantly higher than those in the control group [palpitations or severe palpitation: 92.59% (25/27) vs. 75.87% (22/29), oppressed feeling in chest: 92.00% (23/25) vs. 76.92% (20/26), shortness of breath exhaustion: 90.00% (18/20) vs. 76.19% (16/21), premature beat: 88.24% (15/17) vs. 75.00% (12/16), allP < 0.05], and the comparisons of total effective rates between the two groups including fever with chills [100.00% (15/15) vs. 93.75% (15/16)], deficiency of qi and disinclination to talk [88.00% (22/25) vs. 83.33% (15/18)], dizziness [87.50% (7/8) vs. 80.00% (8/10)], spontaneous sweating or night sweating [86.67% (13/15) vs. 80.00% (12/15)] and body limb ache [91.67% (11/12) vs. 84.62% (11/13)] showed no statistical significant differences (allP > 0.05).Conclusion Junkang oral liquid can significantly improve the clinical symptoms in patients with AVM, reverse its acute course and control arrhythmia, showing the advantage in diagnosis and treatment with combination of traditional Chinese and western medicine.

Objective To investigate the impact of hepatic steatosis on the outcome of adefovir therapy in chronic hepatitis B patients with HBeAg positive. Methods The medical records of 154 patients who were diagnosed with CHB positive for hepatitis B e antigen (HBeAg) and received adefovir therapy for 24 weeks between January, 2011 and December, 2013 were analyzed retrospectively. Baseline status and virologic response, biochemical response and serology response between patient with (n = 68) and without (n = 86) steatosis who were proven by ultrasound. Both groups were given oral adefovir dipivoxil 10 mg/d for 24 weeks , recovery rate in ALT/AST, negative conversion rate of HBV DNA and HBeAg were recorded and compared. Results Recovery rate in ALT/AST in observation group was 67.7%, which is lower than control group (83.7%), and the difference is significant(P < 0.05). Negative conversion rate of HBV DNA and HBeAg in control group is 81.4%and 83.7%, which is significantly higher than observation group (whose values are 58.8% and 44.1%, P < 0.05). Conclusion Hepatic steatosis has significant impact on the outcome of adefovir treatment.

Objective To investigate the cervical human papillomavirus (HPV) infection, genotypes and related high risk factors in 459 women of annual health examination. Methods A total of 459 cervical cytology specimens were de-tected with nest PCR and pyrosequencing methods. Questionnaires were collected simultaneously and the risk factors of HPV infection were analyzed. Results The detection rate of HPV-positive samples was 17.9%. Seven kinds of high-risk genotypes of HPV were found. The detection of HPV 16 was the most common (9.8%), followed by HPV58 (7.0%) and HPV18 (5.2%). The detection rates of simplex infection, dual infections and multiple infections were 9.6%, 4.8%and 1.5%respectively. The risk factors for HPV infection included alcohol consumption,income <3 000 yuan/ month, sexual part-ners>1, frequency of sexual activity>4 times/month and cervical erosion (P<0.05). Conclusion HPV DNA genotyping can provide important reference for HPV screening at early time and the application of HPV vaccines, which also provide sig-nificant evidence for the prevention, diagnosis and treatment of cervical cancer at early stage for the high risk population.

Close attention is paid to WeChat in academic libraries due to its convenience in manipulation, high effec-tiveness in interpersonal communication , richness in content pushing , and accuracy in new pushing .The use of WeChat public platform in academic libraries of Shaanxi Province was investigated .Its opening , popularization and contents were analyzed , which showed that WeChat platform should be constructed in academic libraries by innova-ting its service contents and adding reader experiences .