Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.

Reading therapy in hospital libraries, academic libraries and public libraries was described in terms of its theoretical study and practical study,and its significance in hospital library was elaborated by comparative analysis.

Orthodontic tooth movement (OTM) is a very complicate mechano-biological procedure which is accomplished by the remodeling of periodontal tissues via orthodontic force.As the connective tissue between alveolar bone and cementum,periodontal ligament plays a vital role in the response and conduction of orthodontic force.The susceptible response of periodontal ligament to orthodontic force during OTM is a critical factor on inducing and modulating periodontal tissue restitution.This article reviews the concrete role of periodontal ligament and its mechano-biological behavior during OTM,discusses its mechanical response and biological mechanism respectively on histological,cellular and moleucular levels.

Objective:To investigate the clinical efficacy of loratadine combined with compositus mentholi cream in the treatment of infantile with external ear eczema and evaluate the safety .Methods:Totally 96 cases of infantile with external ear eczema were ran-domly divided into the observation group (n=48) and the control group (n=48) according to a random number table .Both groups were given the conventional treatment .The control group was treated with topical compositus mentholi cream , while the observation group was treated with loratadine additionally .Both groups were treated for 10 d.The clinical efficacy , the level changes of interleukin-4 (IL-4), interferon-γ(IFN-γ) and immunoglobulin-E (IgE) before and after the treatment, the incidence of adverse reactions during the treatment and the relapse after 6-month follow-up were compared between the groups .Results:The total effective rate of the obser-vation group (95.83%) was significantly higher than that of the control group (79.17%, P<0.05); the levels of IL-4, IFN-γand IgE in both groups were significantly decreased after the treatment (P<0.05), and those in the observation group were significantly lower than those in the control group (P<0.05);there was no significant difference in the incidence of adverse reactions between the groups (P<0.05); the recurrence rate in the observation group (8.33%) was significantly lower than that in the control group (22.92%, P<0.05).Conclusion:The clinical efficacy of loratadine combined with compositus mentholi cream in the treatment of infantile with external ear eczema is reliable with promising safety .

The WeChat service provided at present in 14 hospitals directly under the Yunnan Health and Family Planning Commission was surveyed with suggestions proposed for providing active Wechat service in hospitals in order to alleviate the difficulty to see a doctor and the tense relationship between doctors and patients.

ObjectiveTo explore the effect of different nursing models to improve the quality of sleep for elderly patients in department of cardiology.Methods240 cases of patients in department of cardiology in our hospital were randomly divided into the control group and the observation group with 120 patients in each group.The control group was given conventional medical care and the sleep-related health education.While the observation group was given the targeted intervention measures based on the control group,including the provision of good psychological care measures to ensure a good sleep environment,arrange a reasonable sleep time,increase bedtime benign behavior and scientific education of hypnotic drugs knowledge.The PSQI score and sleep quality of different time points were compared.ResultsThe PSQI score of the observation group 1 week and 2 weeks after admission was significantly decreased compared with that upon admission,and was better than those of the control group.The PSQI score of the control group 2 weeks after admission had a marked decline,but the PSQI score 1 week after admission showed no significant difference.In the aspect of sleep quality,the rate of good sleep quality for the observation group 1 week and 2 weeks after admission increased significantly compared with that upon admission.The rate of the quality of good sleep 2 weeks after admission for the control group improved significantly than admission.The rate of promotion for the observation group was obviously better than that of the control group.ConclusionsThe targeted quality of sleep and psychological care interventions for elderly patients in department of cardiology can significantly improve the sleep quality in patients after admission,and enhance their confidence in overcoming the disease,thus contribute to disease treatment and rehabilitation.

Objective To understand cerebral edema and development procedure of different time windows.Methods To analyze their prognosis through image document and operation findings of 52 cerebral trauma patients which were confirmed cerebral edema by CT and operation.Results he light cerebral oedema patients have a better prognosis,10 patients died and 1 patient became a plant man in 11 malignant brain dilatation patients.Conclusion The infernal circle of high brain pressure-cerebral ischemia,cerebral anoxia-cerebral edema result in cerebral edema-brain dilatation-malignant brain dilatation after cerebral trauma which leads to unremitting aggravated pathogenefic condition.So effective means should be used to interrupt the unremitting heightening ICP.

Objective To discuss the correlation between the expressions of high mobility group protein box 1(HMGB1),VEGF-C/D and lymph node metastasis in colon cancer.Methods Forty-five colon cancer samples were stained by immunohistochemical method for VEGF-C/D,HMGB1.The correlations of VEGF-C/D,HMGB1 expressions and lymph node metastasis were analyzed statistically.Results Positive HMGB1 expression was in 33 specimens,lymph node metastasis was in 27 specimens with 23 specimens of positive HMGB1 and non-lymph node metastasis was in 18 specimens with 10 specimens of positive HMGB1.Positive VEGF-C expression was in 27 specimens with 20 in lymph node metastasis.The expression of HMGB1 was consistent with VEGF-C expression,and HMGB1 and VEGF-C expressions were closely correlated with lymph node metastasis,but not with age,sex,cellular differentiation.Conclusion HMGB1 and VEGF-C expression may become predictor to the prognosis of colon cancer patients.

Objective To discuss the clinical effect on treating hysteromyoma with compound acetic acid gossypol tablets.Methods 50 patients who are diagnosed with hysteromyoma take compound acetic acid gossypol tablets 20mg after supper every day for 3 months. Then Monitor the size of uteruses and tumors of the patients,hemoglobinuria routine、liver function、blood sugar、blood ester、follicle stimu- lating hormone、luteinizing hormone、lactation hormone、progestogen hormone、androsterone hormone using B-ultrasound waves before and after treatment.Results patients will have amenorrhea during treatment and the symptom will disappear.The tumor will dwindle in size by 54.66%(P＜0.01)on average.The Hysteromyoma will dwindle by 50.66%(P＜0.05)on average.Also hemoglobin,Hb returns to nom- al.As a result,the blood sugar and lactation harmone reduce a lot than that before treatment.And other monitoring quality doesnt change obviously.Conclusions There is of great effect using compound acetic acid gossypol tablets to treat hysteromyoma and it has no side effect.It is an available conservative treatment.

Looking back to the history of the 20th century contributes to the rise of bioethics and the unification of international law on the present age.In the times of rapid scientific and economic development,it is either a core issue of bioethics or a starting point of international law that how humans should survive and establish a relationship with the nature.And a series of common behavior criteria for human being are being or will being established to seek a co-existent path between peoples as well as between human being and nature.