Objective To explore the management status of long term care hospital nursing workers and find out appropriate countermeasures.Methods By using the methods of questionnaire survey and interview,management status of nursing workers from similar hospitals was investigated.The results were explored and applied combined with the nursing management of our hospital.Results Satisfaction degree of patients and their families about nursing workers increased from 76.7％ to 97.4％.Conclusions It is of key importance that continuous exploration and improvement of hospital nursing workers management be implemented to enhance the high quality nursing of elderly chronic disease hospital.

Objective To investigate the effect of borneol on levels of monoamine neurotransmitters in the prefrontal cortex of rat after long-term continuous operations.Methods A rat model of long-term continuous operations were established by using a treadmill protocol.Then Borneol(0.4 g/kg,p.o.) was given to the rats after the operation,and their activity-rest behavior and active avoidance response were assessed.Two h later,the rats were sacrificed and the level of monoamine neurotransmitter in the prefrontal cortex was determined by high performance liquid chromatography combined with electrochemical detection(HPLC-ECD).Results After the administration of borneol,the activities of operational animals were increased markedly as compared with that of vehicle operational group.The operational rats after borneol application had less response time than the ones treated with vehicle(6.85?1.24) s in active avoidance response.The levels of monoamine neurotransmitters including noradrenaline,dopamine,and 5-serotonin in the prefrontal cortex were decreased after treadmill operation.While application of borneol made the levels of noradrenaline and 5-serotonin increased significantly as compared to operational group.Conclusion Borneol have a reversal effect on rat awake activity impairment after long-term continuous operations.And the arousal effects of borneol are associated with the increase of noradrenaline and 5-serotonin levels in the prefrontal cortex.

Neuroblastoma(NB) is the most common extracranial solid malignant tumor in childhood,which has various clinical manifestations.Tumor metastasis or relapse is one of the major causes of death in patients with NB.At present,the treatments of NB are mainly concentrated on the surgery,chemotherapy,radiation therapy,myeloablative therapy and transplantation,tumor targeted therapy,etc.But the treatment of children with high-risk NB is still not optimistic.Studies have shown that multidmg resistance is a major impediment to the successful treatment of NB.Hence,it is of great significance to have a comprehensive understanding of the chemoresistance mechanisms to improve the survival rate of NB patients.This review discusses the chemoresistance mechanisms of NB in several respects.

So far,the unified definition and diagnostic criteria of feeding intolerance in preterm infants is still unavailable,although they have always been modified and improved.The curative effects of intervention strategies,including the feeding strategies,breast-feeding,medication,and so on,still need multiple clinical randomized studies in order to be verified and evaluated.Therefore,this article will summarize the progress in the definition of feeding intolerance,feeding strategies and medication.

Objective Onto observe the curative effect of carboprost tromethamine injection combined with mifepristone on the treatment of the patients with risky placenta previa. Methods 66 cases with placenta previa during mid pregnancy from January 2016 to December 2016 in Dingbian county people's hospital were randomly divided into two groups, 33 cases in the control group and 33 cases in the observation group. All patients were received cesarean section and traditional treatments such as oxytocin and gauze filling. Hemostatic effect, prognosis and incidence of adverse reactions in the two groups were compared. Results The intraoperative bleeding volume, the amount of vaginal bleeding 2 hours after operation and the amount of vaginal bleeding 2 to 24 hours after operation in the observation group were less than those in the control group (P<0.05). The rate of postpartum hemorrhage, the rate of disseminated intravascular coagulation and the incidence of puerperal disease in the observation group were significantly less than those in the control group (P<0.05). The sanguinous lochia time and menstrual recovery time in the observation group were better than those in the control group (P<0.05). There was no significant difference in the rate of hysterectomy and adverse reactions between the two groups. Conclusion Carboprost Tromethamine Injection combined with mifepristone in the treatment of pernicious placenta previa can effectively reduce the bleeding rate and promote the rehabilitation of pregnant women. It is safe and has no obvious adverse reactions. It is worthy of clinical application.

OATP1B3, a member of SLC superfamily, is specifically expressed on the sinusoidal membrane of hepatocytes and is considered to be important in hepatic drug elimination. The overexpression of OATP1B3 was found recently in tumor tissues such as prostate, colon, and pancreatic tumors. Sequence variations in SLCO1B3 gene, such as SNPs, have been described and a common haplotype consisting of 334T>G and 699G>A SNPs is related to altered transport characteristics of OATP1B3. OATP1B3 is of relevance to drug metabolism through affecting alteration of hepatic concentration of endo- and xenobiotic compounds that interact with nuclear receptors such as PXR and CAR, and thereby directly alter the extent of target gene transcription, including major CYP isoenzymes such as CYP3A4. This review will provide an overview of substrates and inhibitors of OATP1B3 and subsequently to assess the effect of genetic mutation on transport activity. The studies linking OATP1B3 with cancer clinical outcomes are also discussed in this review.

Objective To explore and establish the possible method of low-intensity pulsed ultrasound(LIPUS) irradiation directly combined with SonoVue microbubbles could enhance 5-azacytine (Aza) induced cardiomyogenic differentiation of human bone marrow mesenchymal stem cellin(MSCs)vitro,initially explore the appropriate irradiation condition,and to provide experimental basic data for ultrasound-mediated microbubble destruction application in MSCs transplantation for acute myocardial infarction.Methods (1) In order to screen out the appropriate ultrasound irradiation intensity and time without conspicuous harm to MSCs,stem cells were exposed to different ultrasound intensity irradiation (0.11,0.33,0.55,0.77,0.99 W/cm2) and time (0,30,60,90 s) and then cell proliferation determined by methyl thiazolyl tetrazolium(MTT).In order to screen out the appropriate microbubble-to-cell ratio without conspicuous harm to MSCs,stem cells of different microbubble-to-cell ratios (0,10,50,100,150) were exposed above the screened ultrasound irradiation intensity and time and then cell proliferation determined by MTT.(2) MSCs were divided into four groups and induced cardiomyogenic differentiation by different inducing systems in vitro as follows:no-treatment group,5-Aza inducing group(10 μmol/L),microbubble and ultrasound group,5-Aza inducing (10 μmol/L) with microbubble and ultrasound group,followed by four weeks of further culture.The morphological changes were studied daily under phase-contrast microscope,then the expression of cardiac troponin-T (cTnT) was detected by immunocytochemistry method to analyze and compare differentiation rate of cardiomyocyte-like cells in each group.Results (1)The direct ultrasound irradiation condition of no conspicuous harm to MSCs,but with destruction was intensity of 0.55 W/cm2,exposure time of 30 s and microbubble-to-cell ratio of 50.(2) This study confirmed the feasibility of direct ultrasound exposure of LIPUS on MSCs in vitro.(3) At 28 days after induction,the stem cells of 5-Aza inducing group and 5-Aza inducing with microbubble and ultrasound group expressed cTnT and the positive rate of the latter was higher than the former by immunocytochemistry (13.69％ vs 34.13％,P ＜0.05).Conclusions The appropriate ultrasound direct irradiation condition was intensity of 0.55 W/cm2,exposure time of 30 s and microbubble-to-cell ratio of 50.Appropriate conditions of LIPUS could enhance 5-Aza induced cardiomyogenic differentiation of MSCs in vitro.

The centrosome plays a crucial role in the maintaining of cell conformation,mitosis and chromosome seg-regation.It responds to the DNA damage and keeps the genome stability via cross-talking with the intranuclear DNA damage repair system.The apoptosis of tumor cells can be induced through inhibition of the duplication of centro-some.So the inhibitors of centrosomal proteins will be a potential anti-tumor therapy.

Objective To observe the effect of lowering intraocular pressure(IOP) treatment on ocular hemodynamics in patients with nonarteritic anterior ischemic optic neuropathy (NAION).Methods A total of 68 patients with NAION (68 eyes) were enrolled in this study.The patients were randomly divided into treatment group (38 eyes of 38 patients) and control group (30 eyes of 30 patients).All the patients were received methylprednisolone pulse therapy (200 mg,three days),vasodilator therapy with intravenous infusion of Xueshuantong solution (300 mg),optic nerve nutritional therapy with mouse nerve growth factor (30 μg) and acupoint injection in temporal with compound anisodine (2 ml).The total course was 10 days.The patients of treatment group received IOP lowering treatment to reduce the IOP to ≥ 8 mm Hg (1 mm Hg=0.133 kPa) or in a 30％ reduction.The patients of control group received no IOP lowering treatment.The peak systolic velocity (PSV),pulsatility index (PI) and resistance index (RI) of ophthalmic artery (OA),central retinal artery (CRA) and short posterior ciliary arteries (PCA) before and after treatment were comparatively analyzed by color doppler flow imaging.Results The differences of PSV (t=1.023,1.145,0.569),PI (t=0.679,0.956,1.634) and RI (t=0.816,1.657,0.998) of OA,CRA and PCA before treatment in treatment group and control group were not statistically significant (P＞ 0.05).Compared with before treatment,PSV (t=3.150,7.650,3.520) and PI (t=2.420,5.430,7.650) of OA,CRA and PCA increased obviously (P＜0.05),RI of OA,CRA and PCA decreased obviously (t=5.320,9.640,18.360; P＜0.05) after treatment in treatment group.In control group,the differences of PSV (t=2.090,-2.550,-2.100) and PI (t=-2.310,-2.230,-4.490) of OA,CRA and PCA between before and after treatment were not statistically significant (P＞0.05) ; but the differences of RI of OA,CRA and PCA between before and after treatment was statistically significant (t=2.970,2.160,2.690μ P＜0.05).Compared with control group,PSV (t=2.632,2.135,5.364) and PI (t=3.251,2.432,4.243) of OA,CRA and PCA increased obviously (P＜0.05),RI of OA,CRA and PCA decreased obviously (t=3.664,2.938,4.324; P＜0.05) after treatment in treatment group.Conclusion Lowering intraocular pressure treatment can improve the ocular hemodynamics in NAION patients.

Objection To investigate the effects of etomidate on aorta isolated from IR rats, and explore its underlying endothelium-related mechanism(s). Methods The IR animal model was made by feeding rats with high fructose diet for 8 weeks. Aortic rings were isolated and suspended in a tissue bath, and tensions were recorded isometrically. The effects of etomidate on provoked contractions of the rings were assessed in absence or presence of potassium channel blockers or NO-synthase inhibitors. Results Etomidate-induced relaxation in IR rings was greater than NC rings. NG-nitro-L-arginine methyl ester (L-NAME) or glibenclamide (Gli) inhibit significantly etomidate-induced relaxation in IR rings, and the inhibition of Gli was disappeared in endothelial-denuded aortic rings. Conclusion Etomidate cause vasodilation in IR rat aortas by an endothelial-dependent and independent manner. Impaired NO- and KATP channel-mediated relaxation and etomidate-induced increased availability of may involve in endothelial-dependent relaxation of etomidate in IR rat aortas.