Objective To detect the expression of protein kinase C(PKC) in multidrug resistant cells of colorectal carcinoma after ionizing irradiation and observe the effect of PKC on occurrence and development of the multidrug resistant.Methods The effect of PKC on multidrug resistant HCG-8 cells of colorectal carcinoma after treated with X-ray was detected by indirect immunofluorescence technique and flow cytometry.Results Compared with sham-irradiation group,the positive percentage of PKC was increased significantly(P

Objective To investigate the surgical treatment method for senile gastric cancer complicated with portal hypertension. Methods Retrospective analysis was used for 88 elderly patients who suffered from gastric cancer complicated with portal hypertension.Among the cases,35 cases were treated with standard radical resection,38 cases treated with cytoreductive surgery,9 cases with palliative operation,and 6 cases cured with jejunal fistula.Operation complications,death and mortality,the risk factors of postoperative complications were observed. Results For all the patients,the operation time was (170±33) min,and the intraoperative bleeding was (490±100) ml.In 35 patients (39.8％) occurred operation complications,among which the incidence of incision infection and anastomotic leakage was the highest, which accounted for 14.3％ and 11.4％,respectively.In 5 years,69 patients were in death,the mortality rate was 78.4％ which most resulted from liver failure (21.7％).Logistic regression analysis showed that age,tumor diameter,operation time and perioperation bleeding were the risk factors for death ( 95％ CI:6.92-13.33,7.39-18.26,11.34 24.85,8.69-19.27). Conclusions The surgical approach for elderly gastric cancer patients complicated with portal hypertension should be chosen carefully,otherwise it will bring serious operation complications,and even endanger the lives of patients.

An analysis on the human resources, equipments and budgets of the township hospitals in Wenzhou City discovered the following setbacks: Changeable management system, poor positioning of their functionality, difficulty to play their roles, insufficient funding, inadequate infrastructure, slow development, lack of medical technicians, low level of medical services, and lack of development momentum. Based on these findings, the author proposed the following to upgrade their functionality and promote their development: Clearly positioning their functionality, improving the financial assurance mechanism, deepening system reforms in these hospitals, and further clarifying job divisions.

Purpose:To explore the development change of histopathology of the mouse forestomach neoplasm inducd by B(a)P.Methods:A part of the mice were killed each four weeks after they were given benzo(a)pyrene (concentration 5mg/ml) by lavage for four weeks, twice per week. The histopathology of the forestomach was then observed.Results:Forestomach neoplasm was noticed from the 17~(th) week in the mice. Local gland hyperplasia, arrangement disorder gradually developing into glandular abnormality hyperplasia and then transformed into early stomach cancer and finally into aggressive stomach cancer could be observed.Conclusions:Latent period of the mouse forestomach neoplasm induced by B(a)P is about three months. The development course is atrophic gastritis, atypical hyperplasia and stomach cancer.[

BACKGROUND: The incidence of cognition disturbance after cerebral infarction is about 20% -30% and effective drugs for its prevention and treatment are anticipated.OBJECTIVE: To investigate the effect of nicergoline on cognition disturbance after cerebral infarction and explore its mechanism.DESIGN: Randomized controlled trial based on patients.SETTING: Neurological department in a medical university.PARTICIPANTS: A total of 60 patients admitted to the Neurological Department of the Second Hospital Affiliated to Chongqing Medical University for cerebral infarction during October 1999 and April 2001 were recruited in this study, and randomly divided into two groups, nicergoline treatment group and control group with 30 in each.METHODS: Mini-mental state examination(MMSE) score was evaluated and cerebral blood flow was determined with transcranial Doppler' s ultrasonography within one week after admission and three months after admission, respectively, and data were analyzed with SPSS software.MAIN OUTCOME MEASURES: MMSE score and velocity of blood flow in treatment and control groups.RESULTS: MMSE score in memory, calculation and recollection decreased significantly[ (1.2 ± 1.3), (2. 2 ± 2. 1) and(1.0 ± 1.7), respectively] in control group but did not change much[ (3.9 ± 1.4), (4. 4 ± 1.9) and(4.0 ± 1.6) ]in treatment group. The velocity of blood flow in control group decreased apparently, while it increased in treatment group[(58.31 ±10. 15) and(65.79 ±9.74) cm/s in the right middle cerebral artery].CONCLUSION: Nicergoline can prevent and treat vascular cognition disturbance, and improvement of blood supply may be one of the mechanisms.

Objective:To evaluated the subcutaneous nephrovesical bypass carried out in patients with ureter obstruction and improve the quality of life of patients with palliative definitive percutaneous nephrostomy.Methods:A total of 36 patients with advanced cancer receiving 45 subcutaneous nephrovesical bypass tubes were included in the study.Uronephrosis ranged from 1.8-5.2 cm,serum creatinine 73-760 ?mol/L,and glomerular filtration rate(GFR) 19-26 mL/min.The obstruction was proved to be complete by retrograde pyelography.The nephrovesical stents were inserted utilizing percutaneous access to both of the kidney and bladder,and a subcutaneous tunnel was created between the two sites.B-ultrasound,KUB(kidneys,ureters,and bladder),serum creatinine test and GFR were carried out 72 hours after operation.Results:Urinary diversion operations were successful in the 36 patients,uronephrosis ranged from 0-1.8 cm,all stents were in position,serum creatinine 72-173 ?mol/L,GFR 25-40 mL/min after the operation,and the bypass functioned well in 45 stents for 67 to 481 days of the follow-up.Conclusion:Subcutaneous nephrovesical bypass can protect renal function of patients suffering from ureteral obstruction by simple and minimum invasive operation;it can also reduce complications caused by percutaneous nephrostomy and improve the patient's life quality.

The outbreak of high-pathogenicity avian influenza viruses causing severe illness and death has severely disrupted poultry production and trade and posed a significant threat to public health.In recent thirty years,the virus has been endemic in Southeast Asia,China,Europe and Africa.Although the virus shows little ability of transmission between humans,a process of genetic reassortment could occur in a human co-infected with avian influenza A virus and a human strain of influenza A virus.So H5N1 represents a potential source of the next pandemic influenza.Direct avian-human H5N1 virus transmission is the predominant means of human infection,through direct contact with infected birds and virus-contaminated fomites or with fertilizer containing poultry feces.After several rounds of infection the viruses acquire the mutations required for crossing species barrier and infect a range of mammalian species including humans.Currently,vaccination is still the key strategy to prevent severe illness and death from pandemic influenza.Antiviral drugs such as NA inhibitors play an important role in antiviral treatment.The features of avian influenza virus and its mechanism of transmission in animals and humans are herewith reviewed,and the pathopoiesis of the virus,the current status of vaccine development and antiviral treatment are also discussed.

Objective To study and analyze the efficacy of Metronidazole Suppositories, conjugated estrogen, estrogen ointment and psychological intervention in the treatment of senile vaginitis. Methods 100 cases of senile vaginitis patients in our hospital from February 2015 to August 2016 were selected as the research object. They were randomly divided into the control group and the experimental group, each group had 50 cases. The control group was treated with the treatment of patients in the experimental group of Metronidazole Suppositories, Metronidazole Suppositories, conjugated estrogen ointment in the treatment of psychological intervention on the mental status of patients and patients, strengthen communication and exchanges, increase confidence in the treatment and the treatment compliance of patients. The therapeutic effects of the experimental group and the control group were compared and analyzed. Result After the corresponding treatment, 3 patients in the experimental group were invalid. In the control group, 10 patients were ineffective. The effective rate of the experimental group was 96.0％, which was significantly higher than that of the control group (80.0％), with statistical difference (P<0.05). The incidence of adverse reactions in the experimental group and the control group was 2％ and 4％ respectively, and there was no significant difference. Conclusion Metronidazole Suppositories, combined estrogen ointment and psychological intervention in the treatment of senile vaginitis clinical effect is good, high treatment efficiency, low incidence rate of adverse reaction, has clinical significance.

Objective In order to find a more sensitive and specific noninvasive assay for the diagnosis of bladder carcinoma, the authors tested the exfoliated cells from patient′s voided urine for the presence of telomerase activity and evaluated its clinical significance.Methods PCR-ELISA assay was used to determine the presence of telomerase activity in voided urine samples from patients with bladder carcinoma, fhematuria of benign causes and from normal, healthy volunteers.Results Telomerase activity was detected in 14 of 18 tumor samples, while none of the hematuria samples and normal, healthy volunteers′ samples. Conclusions Telomerase activity could be detected in most urine samples of bladder carcinoma patients and it is more sensitive and specific than other assays, therefore, would be a good diagnostic marker.

As an important in vivo noninvasive molecular imaging modality, PET imaging can quantify and visualize the serial trafficking, tumor targeting, cell number maintenance, cell expansion, activation and immunological function of adoptive immune cells in cancer cell therapy. Thereby it may play a significant role in the treatment options and efficacy evaluation for cellular immunotherapy in cancer. This review focuses on PET reporter gene imaging which has been studied intensively and applied widely in the research on imaging monitoring of cellular immunotherapy for cancer, with the purpose to provide innovative clues for the preclinical study and clinical translation research.