Objective To explore the relationship between Helicobacter pylori (HP) infection and gastric carcinogenesis,and to investigate its mechanism.Methods Totally 333 elderly patients with different degrees of gastric mucosal lesions in our hospital were selected.Patients were divided into 4 groups:chronic superficial gastritis group (n=86),chronic atrophic gastritis group (n=92),gastric ulcer group (n=80) and gastric cancer group (n=75).HP infection in patients were detected by paraffin-embedded tissue sections and ELISA.The gene expressions of c-myc,p16 and p53 were detected by immunohistochemical SP method,and the results were analyzed.Results HP infection positive rate was significantly higher in gastric cancer group than those in the chronic superficial gastritis group and chronic atrophic gastritis group (both P＜ 0.05).There were no significant differences in the expression rates of c-myc,p16 and p53 between HP positive and negative patients in chronic superficial gastritis group (all P＞0.05).There were differences in the expression rates of cmyc and p53 (both P＜0.05),while the expression rate of p16 had no significant difference between HP positive and negative patients in chronic atrophic gastritis group (P ＞ 0.05).There were differences in the expression rates of c-myc,p16 and p53 between HP positive and negative patients in gastric ulcer group and gastric cancer group (all P ＜ 0.05).Conclusions There is a certain correlation between Helicobacter pylori infection and gastric carcinogenesis.It is important to take anti-inflammatory treatment timely and actively to prevent gastric cancer in patients with HP infection in gastric mucosal lesions.

Objective To discuss comparison of different analgesic solution to the immune status and serum levels of inflammatory cytokines of the patients with breast cancer after surgery.Methods The patients of experimental group and control group were given Dizocine and fentanyl to analgesic respectively perioperative experimental.Then analysis and comparison the immune status and serum levels of the two groups.Results The T lymphocyte subsets ( The level of CD3 +、CD4 +and the ratio CD4 +/CD8 +) and the level fo NK cells of the exprimental group patients intraoperative and postoperative 1 d were significantly higer than the control group.The anti-inflammatory factor in the serum of the experimental group patients intraoperative and postoperative 1d was significantly higer than the control group.The proinflammatory factor in the serum of the experimental group patients intraoperative and postoperative 1 d was significantly lower than the control group.Conclusion Dizocine can significantly improve the serum inflammatory factors of the patients with brest cancer after surgery,and can adjust the patient’s immune status.

Objection To investigate the effects of etomidate on aorta isolated from IR rats, and explore its underlying endothelium-related mechanism(s). Methods The IR animal model was made by feeding rats with high fructose diet for 8 weeks. Aortic rings were isolated and suspended in a tissue bath, and tensions were recorded isometrically. The effects of etomidate on provoked contractions of the rings were assessed in absence or presence of potassium channel blockers or NO-synthase inhibitors. Results Etomidate-induced relaxation in IR rings was greater than NC rings. NG-nitro-L-arginine methyl ester (L-NAME) or glibenclamide (Gli) inhibit significantly etomidate-induced relaxation in IR rings, and the inhibition of Gli was disappeared in endothelial-denuded aortic rings. Conclusion Etomidate cause vasodilation in IR rat aortas by an endothelial-dependent and independent manner. Impaired NO- and KATP channel-mediated relaxation and etomidate-induced increased availability of may involve in endothelial-dependent relaxation of etomidate in IR rat aortas.

Objective To investigate the relationship between TCM syndrome of hyperlipidemia and indexes such as age,sex,BMI,TC,TG,LDL-C,HDL-C,ApoA,ApoB,the vascular media,coagulation and inflammatory indices.Methods 200 patients of hyperlipidemia recruited from the cadre ward in hospital were made TCM syndrome differentiation according to Guiding principles for the clinical study of Chinese Medicines.SPSS16.0 statistical software were adopted for analyzing the relation between TCM syndrome and indexes such as age,sex,BMI,TC,TG,LDL-C,HDL-C,ApoA,ApoB,the vascular media,coagulation and inflammatory indices.Results ①)There are no differences TCM syndrome and the indexes of age,sex,TC,TG,HDL-C,LDL-C,VLDL-C,lipoproteins,the vascular media,coagulation and inflammatory indices.②Compared with the liver kidney yin deficiency syndrome group,apolipoprotein A was higher in the groups of qi stagnation and blood stasis,phlegm stagnation syndrome,and hyperactivity of Yang due to yin deficiency syndrome,the difference was statistically significant; while APOB showed no difference among the groups.Conclusion TCM syndrome of hyperlipidemia had no relation to indexes of age,sex,body mass index,blood lipid,and blood coagulation.The study found a correlation between hyperlipidemia and phlegm and Qi deficiency,which can provide a theoretical basis for syndrome differentiation of TCM.

Objective To investigate the risk factors and their prevention and treatment approaches for occurring radial artery spasm (RAS) during the brain digital subtraction angiography (DSA) with transradial catheterization in patients with ischemic cerebrovascular disease.Methods The patients who underwent DSA of transradial approach because of ischemic cerebrovascular disease were enrolled.Their clinical data,surgical complications,and radial artery spasm were collected.The data of occurring RAS or not during DSA of transradial approach in patients were compared.Multivariate logistic regression analysis was used to analyze the risk factors for occurring RAS during DSA of transradial approach.Results A total of 100 patients with ischemic cerebrovascular disease who underwent DSA of transradial approach (74 ischemic stroke,26 transient ischemic attack) were enrolled,of which 21 (21％) had RAS.Of the 21 patients with RAS,4 suspended their operations and completed their DSA after spasm relief; another 17 were treated with intrathecal cocktail (heparin 2500 U,nitroglycerin 0.5 mg,verapamil 1.25 mg) and completed their DSA after spasm relief.There were significant differences in the proportions of radial artery diameter (1.89 ±0.28 mm vs.2.12 ± 0.17 mm; t =1.582,P =0.041),duration of operation (12.3 ± 3.1 min vs.9.8 ±2.7 min; t =1.264,P =0.038),and radial artery diameter ＞2 mm (9.52％ vs.65.82％ ;x2 =9.624,P =0.002),radial artery anatomy variation (23.81％ vs.0.27％; x2 =14.185,P＜ 0.001),and the first successful puncture (42.86％ vs.78.48 ％ ;x2 =5.335,P =0.021) in patients between RAS group and the non-RAS group.Multivariate logistic regression analysis showed that the radial artery anatomy variation (odds ratio [OR] 1.940,95 ％ confidence interval [CI] 1.372-2.241; P=0.023) was an independent risk factor for the occurrence of RAS.Radial artery diameter ＞2 mm (OR 0.752,95％ CI 0.352-0.847;P=0.043) and the first successful puncture (OR 0.843,95％ CI 0.367-0.941; P=0.045) were the independent protective factors for RAS.Conclusions RAS is a common complication of DSA of transradial approach.Repeated puncture,radial artery diameter,and radial artery anatomy variation are its most important influencing factors.

Objective:To investigate ?-catenin expression during check pouch carcinogenesis in golden hamster.Methods:Expression of ?-catenin was examined with immunohistochemical SABC method in 23 cases of check normal buccal epithelium of golden hamster,22 of hyperplasitic epithelium,22 of dysplastic epithelium,29 of squamous cell carcinoma.Results:Normal buccal epithelium showed strong pericellular staining in the basal,suprabasal and prickle cell layers.Positive rates(%) of ?-catenin in normal buccal epithelium,hyperplastic epithelium,dysplastic epithelium and squamous cell carcinoma were 100,100,77.3 and 41.4 respectively.Conclusion: The decreased expression of ?-catenin may be related to the carcinogenesis of golden hamster check pouch.

Objective To study the clinical characteristics of the severe acute respiratory syndrome(SARS) patients who progressed into multiple organ injury Methods Retrospective analysis was performed on the clinical data of 54 severe SARS patients with multiple organ injury in our hospital Results In 54 SARS patients,systemic inflammatory response syndrome(SIRS) developed in all cases and multiple organ injury developed in 49 cases and the mortality of patients with multiple organ injury was 22 2% Among the involved organs,lungs were the most frequently involved organ,followed by liver,heart,brain,kidney and blood system The injury in other organs was rare Conclusions Serious SARS is predisposed to progress into multiple organ injury The mortality was correlated to the number of organs involved Recognization and treatment of multiple organ injury at early stage are critical with improve cure rate of serious SARS with multiple organ injury

Purpose To summarize the CT features and clinical characteristics of atypical primary fallopian tube cancer (PCFT) and ovarian cystadenocarcinoma (OCA), in order to improved the diagnosis. Materials and Methods CT findings and clinical data of 12 patients with atypical PCFT (PCFT group) and 20 patients with OCA (OCA group) were retrospectively studied. All patients were confirmed by pathology. Serum CA125 level, clinical characteristics and CT features of two groups were compared. Results Serum CA125 level of PCFT group and OCA group were (486.13±23.89) U/ml and (1606.94±62.86) U/ml, respectively. There was statistic difference between the two groups (P<0.01). There was significant differences on vaginal bleeding and vaginal discharge between the two groups (P<0.01). There were 4 cases with solid mass, 8 cases with cystic solid mass in PCFT group. And in OCA group, 16 cases with cystic solid mass and 4 cases with cystic mass were revealed, which showed statistic differences between the two groups (P<0.05). Some other findings also showed statistic differences including the diameter of ovarian vein on the affected side, the diameter of the round ligament of uterus on the affected side, tumor volume, and CT values of the mass in venous phase and delay phase (P<0.05 or P<0.01). However, there were no statistic significance in the size of metastatic lymph nodes, the mean CT value of the mass on plain scan and in the arterial phase (P>0.05). Conclusion Clinical findings of serum CA125 level, vaginal bleeding, and vaginal discharge combine with imaging findings of diameter of the ovarian vein in the affected side, diameter of ipsilateral round ligament of uterus, tumor volume, CT values of the mass in venous and delay phase would be the key points for differential diagnosis of PCFT and OCA.

A total of 25 fast-growing rhizobium isolates from variety of legumiuous plants in Hainan Province, a tropical region of China, and 7 representative strains of genera Rhizobium, Sinorhizobium and Agrobacterium were characterized by numerical taxonomy. The results indicated that rhizobium isolates from Hainan were taxonomically heterogenous. At thesimilarity level of 70%, 13 strains from Hainan formed a distinct group which could use wider range of carbon and nitrogen sources, and were more resistent to antibiotics and chemicals than known rhizobium species. 2 strains could not fall into any group, and the others fell into different known species respectively. There were no correlations between hosts and the taxonomic positions of their symbionts. The rhizobium isolates from the same host genus or speceis could fall into different groups.

Objective:To construct recombinant expressing vector pcDNA3-DAF and to develop the NIH3T3 cell model expess human complement regulatory protein decay accelerating factor(DAF,CD55)stably after transfected.Methods:Human membrane complement regulatory protein(hCRP) DAF cDNA containing the full-length of encoding region was cloned into expressing vector pcDNA3.After identification by restriction enzyme digestion,PCR and sequencing,the recombinant plasmid was transfected into NIH3T3 cells with calcium phosphate-DNA precipitate method.A stably-transfected cell line was established by G418 selection.Extraneous gene integration was identified by PCR.Expression of DAF at both mRNA and protein levels was analyzed by RT-PCR,Western blot and indirect immunofluorescence microscopy.Results:The eukaryotic expression vector pcDNA3-DAF was successfully constructed and the DAF gene was transfected stably into NIH3T3 cells,a stably-transfected cell line was established and DAF was efficiently expressed on the surface of transfected NIH3T3 cells.Human DAF cDNA was integrated into NIH3T3 pcDNA3-DAF genomic DNA after continuous 30 times passages,indicating that NIH3T3 pcDNA3-DAF was stable cell line.Conclusion:The establishment of the stably-transfected cell line and the expression of the target gene provide a base for further studies on the function of the DAF and the cooperative fashion among different human complement regulatory proteins in alleviating the complement-mediated cytolysis.