Objective:There are many different kinds of biological safety cabinets, it make some difficulties in the allocation. Scientific selection which based experimental requirements choose the right equipment match experiments, it can effectively prevent the spread of pathogenic microorganisms of operation personnel damage and environmental pollution.Methods: According to its working characteristics, different laboratory levels, detection items and actual operation of dangerous levels of pathogenic microorganisms, to determine the matched biological safety cabinet.Results: According to the different experiments correctly, choose the appropriate biological safety cabinet, bio-safety has been obviously improved, the incidence of adverse events decreased from 12% to 3%; the experimenter's security has improved.Conclusion: Through the use of biological safety cabinet clear and selection method, can not only improve biological testing of safety consciousness, but also strengthen the biological safety standardization, institutionalization and standardization of the management mechanism, avoid the use of biological safety, improve the quality of the efficiency of laboratory work and experiment, laboratory personnel biological safety.

Hg.The health risk of gene toxic substances was more distinct than that body toxic substances.(2)The total health risk of all pollutants in drinking water was 7.18?10-5a-1 in 2003,2.12?10-4a-1 in 2004 and 2.28?10-4a-1 in 2005,all of which exceeded 5.0?10-5a-1,the value recommended by ICRP,and greatly exceeded the standards recommended by the Sweden Bureau of 2 Environment Protection and the Holland Ministry of Building and Environment Protection.Conclusions:There is reference value in our research for risk management of drinking water resources and water enviroment poretcting measures.

Objective To clarify the association of age and sun exposure with human epidermal thickness, and to probe deeply into the changes of skin histology and epidermal thickness with skin aging.Methods Two hundreds and eleven full thickness skin samples were collected from 3 different age groups of healthy volunteers and then stained with haematoxylin and eosin to study the changes of epidermal thickness using image analysis software ( Image pro plus 6.0). The obtained values were analyzed with factorial design ANOVA and correlation analysis to detect any significant effects of age and sun exposure on epidermal thickness.Results With increasing age, the junction between epidermis and dermis became flattened, and stratum comeum got looser. There was a notable attrition of the epidermal thickness that could be correlated with age. The at-trition of epidermal thickness attributed to age did not vary between female and male volunteers. Epidermal cell layers thickness increased with cumulated sun exposure, but epidermal total thickness was not associated with sun exposure. There was no difference for epidermal thickness of different sun exposure between female and male volunteers. Conclusion During chronological aging, epidermal cells decrease in thickness with lowered metabolism. With cumulated sun exposure, epidermal cell layer thickness becomes thicker. There is no interaction between the effect of age and sun exposure and the epidermal thickness.

Objective: Analysis of adult attachment in a very stressful situation, i.e., surgery. Methods: Two widely used adult attachment measurements --Relationship Questionnaire(RQ) and Experiences in Close Relationship Inventory(ECR)-are administered to 50 surgery patients in a hospital. Their personal information is also collected. Results: The distribution of 50 patients' adult attachment is: 18(36%) of them are secure, 20(40%) Dismissing, 6(12%) Preoccupied, 6(12%) Fearful. Moreover, attachment anxiety measured by ECR has negative correlation with model-of-self measured by RQ (P

0.05),but the positive ratio of 16SrRNA genes expression in PB in Group C and Group E was significantly higher than that in group B and Group D respectively(P0.05).Conclusions(1)Detecting 16SrRNA genes from PB with PCR has certain significance for early diagnosis of jejunal anastomotic leakage and ileal anastomotic leakage,(2)PCR might be a useful tool for early diagnosis of jejunal anastomotic leakages and ileal anastomotic leakages by detecting lacZ genes or 16SrRNA genes from ascites.

Objective To asses the value of detecting bacterial DNAs in rat's blood with PCR for early diagnosis of colonic anastemotic leakage.Methods 48 healthy female Wistar rats were random divided into three groups: Group A(n=8,sham operation group),Group B(n=20,colonic anastomosis group),Group C(n=20,colonic anastomotic leakage group).Group B and C rats underwent standardized colon resection 3cm away from the ileocecal junction 10cm,Group B rats were done with a complete anastomosis(end-to-end single layer anastomosis with 0# silk sutures) while Group C rats were done with an anastomosis leaving a 5mm opening in colonic anterior wall.lml and 3ml venous blood samples were collected from Group A,B and C.DNAs were extracted from these blood samples and PCR techniques were used to amplify lacZ genes from Escherichia coli and 16S ribosomal RNA genes(16SrRNA genes) 3 days after operation.The data were analyzed by chi square test.Specimens of the experimental intestine were HE stained for pathological studies.Results The positive ratios of expressing lacZ genes in peripheral blood(PB) with PCR in Group C were significantly higher than that in Group B(P<0.05),hut there were no differences between the two groups in expressing 16SrRNA genes(P>0.05).Conclusions It could be a useful way to detect lacZ genes of Escherichia coli from PB by PCR but not 16SrRNA genes for diagnosis of colonic anastomotic leakages.

The incidence of poststroke dementia is high and the burden of disease is heavy. It is very important to prevent or delay its occurrence and progression. In recent years, the lifestyle adjustments, such as the intervention for vascular risk factors, adhering to the diet management, and functional exercise have been confirmed to prevent poststroke dementia to a certaln extent. Acetylcholinesterase inhibitor and glutamate receptor antagonist, etc. play an important role in delaying the progression of poststroke dementia and vascular cognitive impalrment. This article reviews the latest progress in research on the prevention and treatment of poststroke dementia.

Idiopathic thrombocytopenic purpura (ITP),as a heterogeneous autoimmune disease,is the most common bleeding disorder,of which etiology and pathogenesis are still unclear. Health related quality of life are severely affected,especally in chronic patients . Studys on chronic idiopathic thrombocytopenic purpura pathogenesis ,both in China and abroad,have found that genetic factors may be involved in the occurrence and prognosis of the disease. The paper gives a review from the follow aspects,such as its risk factors,relationship between genetic polymorphisms or expression levels and chronic ITP susceptibility and seriousness,the role of epigenetic,researchs about chronic and refractory ITP,et al.

Objective:To solve the key material quality of blood control requirements, quality and strengthening the key material confirmation, control of nonconforming products produced, the introduction of the Deming cycle method (PDCA) for process management, improve the management level, to ensure the safety of blood transfusion work. Methods: In accordance with the relevant laws and regulations made provisions for key materials management in blood station and operation guide, the use of PDCA circulation method in daily work carried out the whole quality tracking management. Design quality control scheme is feasible and measures, measures for effect before and after the implementation of the analysis and comparison of the. Results: The quality control through the key materials of the blood, the blood bank purchased key material input of qualified acceptance rate and quality department quality sampling pass rate reached 100%. Conclusion:The responsibility of quality control key material major, quality control should be implemented, through the whole process of fine tracking management, and continuously improve the management method, ensure the blood quality, blood safety and the safety of clinical blood transfusion.

The prevention,diagnosis,treatment,and recovery of serious diseases can't be well done without the family's proper awareness and wisdom of choices.The target population of the prevention and control of serious diseases and health education should take a family as the basic unit and let the family become the first line of defense against the serious diseases.The idea and practice of Healthy Family has established a strong bond between the Healthy China strategy and the broad masses of the people,helping achieve the bottom-up interconnection and promoting Healthy China 2030 to benefit all.