Antioxidants ; metabolism ; therapeutic use ; Bronchopulmonary Dysplasia ; drug therapy ; etiology ; metabolism ; Humans ; Hyperoxia ; complications ; Infant, Newborn ; Lung ; metabolism ; Reactive Oxygen Species ; metabolism

ObjectiveTo obtain the soluble expression of P protein of norovirus(NV)G Ⅱ. 2[P16]and analyze its antigenic characteristics.MethodsThe recombinant plasmid GⅡ.2-NV-pET-28a was constructed by optimizing the NV GⅡ.2[P16]P protein gene,synthesizing and cloning it into pET-28a(+)vector. The recombinant plasmid was transformed into DH5α competent cells,and the expression strain was optimized to make the P protein express in soluble form. The expressed P protein was purified by Ni-NTA affinity chromatography,of which the antigenicity was detected by Western blot,the purity was detected by size exclusion high performance liquid chromatography(SEC-HPLC),and the antigenantibody binding ability was identified by ELISA.ResultsThe target protein of recombinant BL21 Star(DE3)pLySs was highly expressed in soluble form. After purification by affinity chromatography,the purity of GⅡ.2 P protein was 95. 53%,and the antigenicity was good.ConclusionSoluble NV P protein was successfully prepared by prokaryotic expression strain,and the antigenicity of P protein was confirmed,which laid a foundation of the development of NV G Ⅱ.2[P16]detection kits and the recombinant subunit vaccines.

Objective To observe the change of serum Omentin-1 in gestational diabetes mellitus(GDM)and to investigate its clinical significance.Methods Serum Omentin-1 level was detected in 85 cases of GDM and 85 matched cases of normal glucose tol-erance(NGT)by ELISA,the glycolipids biochemical indexes,inflammation indexes and fasting insulin (FINS)level were simultane-ous detected,then the insulin resistance index (HOMA-IR)was calculated in both groups.Results The levels of prenatal prepreg-nant BMI,FPG,hs-CRP,blood lipids,blood glucose,FINS and HOMA-IR in the GDM group were significantly higher than those in the NGT group,while serum Omentin-1 level was obviously lower than that in the NGT group,difference was statistically signifi-cant (P<0.05);when prenatal obesity and/or HOMA-IR≥2,the serum Omentin-1 levels was significantly decreased.Serum Omentin-1 level had significant positive correlation with HDL,and negative correlation with prepregnant BMI,prenatal BMI,FPG, FINS and HOMA-IR.The multiple stepwise regression analysis showed that prepregnant BMI,TG,FPG and FINS were independ-ent influencing factors of serum Omentin-1 in GDM.Conclusion Serum Omentin-1 is associated with GDM closely,which can re-flect the degrees of sugar,lipid metabolic disorder and insulin resistance in pregnant women,and may be involved in the occurrence and development of GDM.

Objective To study the pathogenic molecular mechanism of H.pylori through analyzing its effect of on MMP-9 expression. Methods BGC-823 cells were cocultured with ATCC49503(CagA +)and HP 030811 (CagA -) H.pylori strains respectively, then the total cellelar RNA was extracted. The MMP-9 mRNA expression in BGC-823 cells, 9 cases of H.pylori-positive and 9 cases of H.pylori-negative gastric ulcer biopsy tissues was detected by RT-PCR. The MMP-9 protein expression on the paraffin-imbedded tissue sections of 17 cases of H.pylori-positive and 14 cases of H.pylori-negative gastric ulcer tissues was detected with immunohistochemical technique, and was quantified by image quantitative analysis. Results H.pylori induced MMP-9 expression in BGC-823, and the level of MMP-9 expression induced by CagA -postive H.pylori strain was higher than that by CagA -negative one. MMP-9 expression level was higher in the H.pylori-positive gastric ulcer tissues than that in the H.pylori-negative ones. Conclusion H.pylori-induced MMP-9 expression may play an important role in gastric ulcer formation and gastric carcinogenesis.

Conventional therapy was given to 260 cases of advanced stage of cancer of colon during the period of 1975 - 1988. Radical operation, palliative resection or changing bowel passage were selected according to the condition of the lesions. Post operative chemo -therapy with integrated Chinese and western medicine were given, or with radiotherapy in some of the cases. Results showed that the 5- year survival rates of II, III, IV stages were 80. 5%, 56. 12%, 21. 73% respectively, the mean 5 -year survival rate being 52. 78%, 45 cases with liver metastasis were also treated with integrated medicine with a 5 - year surivial rate of 22. 2%. The above figures of therapeutic effect and survival rates were all superior to that in cases treated by western medicine alone as reported by domestic exponent units.

The medicine of Yi ethnic plays an important role of the traditional medicine of China. Recently, the facility of Yi ethnic medicine has made great progress on its development process. Now many medicine industries develop the production of Yi ethnic medicine. Thus, this demands a great quantity of Yi ethnic medicine, but it also leads rapid decline with overuse of the medical resources. Here we discussed a series of problems, and put forward the corresponding stradegies of protection of Yi medicine.

Objective To study the value of the ovarian cyst treated with sclerotherapy under CT guiding. Methods Forty-five cases with ovarian cyst treated with sclerotherapy under CT guiding. Results 36 cases were all cured and other 8 cases reduced and 1 cases was not changed. Conclusion This study suggests that the method of ovarian cyst treated with sclerotherapy under CT guidance is uncomplicated and curative effect.

Objective To investigate whether cocktailtherapy combined with of neuroprotectants may have more advantages over single agents in treating focal cerebral ischemic cascade Methods Use suture occlusion technique, the right middle cerebral artery in rats was occluded Tirty minutes later,Fructose 1,6 diphosphate(FDP)(50 mg/kg, n =20), MK 801(1 mg/kg, n =20) and N acetylcystein(NAC)(150 mg/kg, n =20) were singly or combinantly infused intraperitoneally At the same time the cocktail treated group ( n =20) were infused the above agents combinationtly and the control group ( n =20) were infused normal saline intraperitoneally. six and 24 hours after focal cerebral ischemia,the animals were weighted and neurologically assessed on 5 point scale The animals were killed; brain were stained with 2,3,5 triphenylte trazolinm chloride for assessment of the volume of infarction, and then embedden onto slides with paraffin for morphological assessment and terminal transferase dUTP nick ending labeling(TUNEL) were carried out for apoptosis and immunohistochemistry were carried out to investigate the changes in bcl 2 Results All neuroprotectants decreased the volume of infarction( P

Objective] To sum up Pro. Li Saimei’s clinical experience in treating mental diseases. [Method] By following the teacher clinic and sorting out the related medical materials,the author summarizes professor Li Saimei 's academic experience of treatment of mental diseases ,and for proven cases. [Result] In professor Li Saimei's opinion,mental diseases are due to dysfunction of shaoyang's turning,and phlegm blocking the spirit orifices. Professor Li usually uses Chaihu related formulas to turn the Shaoyang,resolve the phlegm,open the three Jiao,regulate Yin and Yang.The notable clinical result has been observed.[Conclusion] Professor Li Saimei's experience is effective and worthy of inheritance and promotion.

Objective To investigate the predictive value of epicardial adipose tissue(EAT) thickness for the recurrence of atrial fibrillation (RAF) after concomitant valve replacement and bipolar radiofrequency ablation.Methods From January 2012 to May 2014,156 patients with permanent atrial fibrillation and organic valvular diseases underwent concomitant valve replacement and successful bipolar radiofrequency ablation in the First People's Hospital of Shangqiu.After 1 year of follow-up,the recurrence of AF was analyzed and the correlation factors were analyzed statistically.Results All patients completed a regular follow-up.During a 12-month follow-up period,RAF was observed in 39 patients (25.0％).Echocardiography-derived regional EAT thickness was significantly greater in RAF patients than that in non-RAF patients((6.9±0.6) mm vs.(5.5±0.7) mm,t=4.649,P＜0.05).The EAT thickness strongly correlated with patients age,body mass index,Ratio of left atrial diameter,mitral diastolic blood flow velocity and early diastolic velocity of mitral annulus,low density lipoprotein-cholesterol and thyroglobulin (r =0.62,0.57,0.68,0.59,0.73,0.76;P ＜ 0.05).Cox multivariable analysis showed that,duration of AF history (95％CI:1.01-1.15,P =0.040),left atrial diameter (95％CI:1.07-1.24,P =0.021) and EAT thickness (95％CI:1.05-1.79,P =0.009) were independent predictors of RAF.Conclusion Echocardiography-derived regional EAT thickness combined with left atrial diameter may serve as effective parameters in identifying patients at the highest risk for RAF.