Objective To observe the changes of serum gastrin (GAS)and plasma motilin (MTL)in patients with primary bile reflux gastritis.Methods 80 patients with primary bile reflux gastritis were selected.The serum GAS and plasma level of MTL were observed,and the results were compared with 80 healthy people.Results The content of MTL in plasma of the study group (282.37 ±31.56)pg/mL was significantly lower than that in the control group (t =-21.352,P <0.01).The level of serum GAS (91.28 ±27.71)pg/mL was significantly higher than the control group (t =-28.388,P <0.01).Conclusion Compared with the healthy people,secretion of MTL in patients with primary bile reflux gastritis has insufficient phenomenon,and has the excessive secretion of GAS in the body,this may be a factor in the pathogenesis of the disease.

Objective To investigate the curative effects of B-ultrasound guided radio frequency therapy in the treatment climacteric dysfunctional uterine bleeding. Methods B-ultrasound guided radio frequency therapy was adopted in 121 cases of dysfunctional uterine bleeding with an exclusion of malignant lesions. Results The operation time was 6 min～12 min, with a mean of 8.5 min. No adverse effects were observed. The cure rate and marked effective rate were 88.4% (107/121) and 11.6% (14/121) in the 3rd postoperative month; the cure rate, marked effective rate and effective rate were 78.3% (36/46), 19.6% (9/46) and 2 1% (1/46) in the 24th postoperative month, respectively. Conclusions B-ultrasound guided radio frequency therapy in the treatment of climacteric dysfunctional uterine bleeding is simple and minimally invasive, being highly recommended.

Objective: To investigate the effect of Trichostatin A (TSA) on histone acetylation and expression of ING1b mRNA in Colo205 human colon cancer cell line. Methods: Human colon cancer Colo205 cells were cultured and divided into 4 groups. Cells in the control group (group A) was treated without TSA. In the other three groups, cells were treated with 3 different concentrations of TSA: 50μg/L (group B), 100μg/L (group C), and 200μg/L (group D). At 24 hours after treatment, the level of histone H3 acetylation was analyzed by chromatin immunoprecipitation (CHIP) and the expression of ING1b mRNA was detected by RT-PCR with qPCR. The growth of Colo205 human colon cancer cells in group C and D was obviously inhibited compared with that in group A and B. Results: The Ct value of histone H3 acetylation and mRNA expression of ING1b in group A were 23.25± 0.08 and 23.32±0.05, respectively. After treatment with TSA, the 2~(-△△Ct) value of histone H3 acetylation in group B, C, and D were 1.12, 4.21 and 4.38, respectively. The level of histone H3 acetylation in group C and D was increased more compared with that in group A (P<0.05) and there was no difference between group B and group A (P>0.05). The 2~(-△△Ct) value of the expression of ING1b mRNA in group B, C and D were 1.33, 4.52 and 4.62, respectively. The expression of ING1b mRNA in group C and D were more than that in group A (P<0.05). Group B and group A had a similar level of ING1b mRNA expression (P>0.05). Conclusion: The histone acetylation is probably responsible for ING1b expression silencing in Colo205 human colon cell line. TSA at 100μg/L can increase the level of acetylation and activate the gene transcription which is silenced by low level of acetylation and induce the expression of gene, inhibiting the growth of tumor cells.

objective To investigate the diagnosis and treatment of hepatic focal nodular hyperplasia(FNH).Methods Twelve cases with hepatic focal nodular hyperplasia from January 2003 to December 2006 in our hospital were reviewed retrospectively. Results Three cases had epigastric discomfort(25%),5 cases with elevated ALT(41.67%),3 cases with elevated AST(25%),2 cases with elevated AFP(16.67%),one patient was of positive HBsAg(8.33%).The rate of preoperative correct diagnosis made by ultrasound,CT and MRI was 0,20%and 25%respectively,there was no significant difference between CT and MRI.Final diagnosis was established by stlrgical resection and pathology in 11 cases and by needle biopsy in one case.All cases recovered and were followed-up with an average of 28 months.No recurrence was found in cases undergoing surgical resection. Conclusion The rate of correct preoperative diagnosis for FNH is low,definite diagnosis depends on pathologic examination.The prognosis is good,however,most patients in this series underwent resection because of possible malignancy especially in eases of cirrhotics.

Objective: To estabish an animal model with blast injury in maxillofacial region and to study the biomechanical property of the model.Methods: Spherical explosive at different distances was used to make wound in maxillofacial region in dogs. The pressure on tissue at different level, the variation of horizontal acceleration and strain of the mandibular and temporal bone were dynamically measured.Results: About 3.5 cm?3.0 cm soft tissue defect and madibular splintered fracture occurred in maxillofacial region when the spherical explosive exploded on the surface of the dog's face and the animal survived. The pressure on mandible was respectively 3.6%?2.9% of the surface of dog's face when the spherical explosive exploded 0 and 1.00 cm away from the dog's face while the ratio of horizontal acceleration of cranium to mandibular was 1/8?1/6. Splintered fracture occurred in mandible when the mandibular strain was 11 375 ??.Conclusion: The model can be applied as an animal model with blast injuries in maxillofacial region.

Objective: To study the injury characteristics of blast injury in maxillofacial region wounded by spherical explosive. Methods: Spherical explosive was used to wound maxillofacial region in rabbits , after gross observation of the trauma , bone tissue specimens were collected from 0.5 or 1.0 cm to the boundary of wound 6 hours, 3 and 7 days after wounding respectively, the samples were prepared and stained with HE and modified Mallory's trichrome staining and observed under light microscope . Microcirculation in wounded bone was studied by ink injection and observed in ground bone sections. Results: No changes were found 6 hours after injury. Inflammatory infiltration were found 3 days after injury.Osteonecrosis was observed 7 days after injury, the structure of bone beyond 0.1 cm of the wound was found to be normal and restoration of bone was observed. Conclusion : Pathological changes of mandible injured by spherical explosive are similar to those of usual fracture except that the wound of blast injury is seriously polluted.

Objective To investigate the effect of high intensity focused ultrasound(HIFU) on the immunity of patients with advanced primary liver cancer(PLC).Methods Forty cases of PLC admitted to our institution from Mar.2003 to Dec.2003 were included in this study.Patients were divided into 2 groups and received either HIFU or radio-frequency ablation(RFA) treatment randomly.CD3,CD4,CD8,CD4/CD8,NK,IL-2,TNF were chosen to assess the immune status before and after treatment.The results were compared statistically.(Results The) survival rate after HIFU was 80.0%,61.1%,42.9%,33.3% at 3 months,6 months,9 months and 1 year respectively,which was similar to that after RFA treatment.The changes of immunity parameters of CD3,CD4,CD8,CD4/CD8,NK,IL-2 and TNF were not significant after HIFU treatment.In addition,the differences of those parameters between HIFU group and RFA group were insignificant.Conclusion There are no detrimental effects on immunity in the early period after HIFU treatment.

OBJECTIVE To investigate the protective effect and its frequency selection of dopamine on the inner cell in cochlea when exposed to white-noise;In order to offer an important step on understanding of negative protective modulation of dopamine in the cochlear auditory afferent nerve. METHODS Forty guinea pigs were randomly divided into 4 groups and whole intracochlear perfusions were performed. ① exposed to the 100dB white-noise; ② perfused with artificial perilymph solutions; ③exposed to the white-noise and perfused with artificial perilymph solutions; ④exposed to the white-noise and perfused with Artificial perilymph solutions containing 1mmol/L dopamine. Compound action potential(CAP)evoked by different frequencies tone pip, and cochlear mirophonics(CM)evoked by 4kHz tone burst were recorded from the round window of guinea pigs before and 2h after perfusion. RESULTS In the groups exposed to the white-noise, the CM amplitude and the linearity of input-output function both significantly decreased at 2h(P

OBJECTIVE To analyze 1000 Hz probe tone tympanometry of middle ear with different volume of fluid, and to evaluate the role of 1000 Hz probe tone tympanometry in the diagnosis of presence of fluid in tympanic cavity. METHODS Tympanometries with 1000 Hz or 226 Hz probe tone were obtained from all the guinea pigs with different volume of fluid in tympanic cavity using GSI33 analyzer and tympanometry curves were analyzed. RESULTS There was a significant difference of the peak pressure of 1000 Hz tympanometry among all test groups and control group. The peak pressure decreased with the increasing of fluid volume in tympanic cavity. CONCLUSION Tympanometry with 1000 Hz probe tone can be used in the diagnosis of fluid in tympanic cavity, and it can be an indicator of volume of fluid in tympanic cavity.

Objective To investigate the effects of Buyanghuanwu decoction on infiltration of neutrophils and expression of ICAM-1 after middle cerebral artery occlusion (MCAO) in rats. Methods Rats were pretreated with Buyanghuanwu decoction(13 g/kg and 26 g/kg) for 7d and then subjected to cerebral ischemia/reperfusion injury induced by an MCAO. After a 90min ischemia and a 24h reperfusion, the neurological deficit and the survival neurons were determined. The effects of Buyanghuanwu decoction on infiltration of neutrophils and expression of ICAM-1 by immunohistochemistry are evaluated. Results Buyanghuanwu decoction significantly ameliorated the neurological deficit and increased survival neurons . The myeloperoxidase (MPO) positive cells and the ICAM-1 positive vessels in the vehicle-treated rats were increased significantly (P