BACKGROUND: Donor antigen presenting cells immigrated into the recipient can induce the immunological tolerance of recipient T cells to donor, leading to a final acceptance to grafts, lnterleukin-10 (IL-10) modification maintains dendritic cells at a desirable differentiating state, which is an effective method to promote the protection to kidney in the simultaneous liver-kidney transplantation.OBJECTIVE: To observe the immigration of IL-10-modified dendritic cells in rats subjected to simultaneous liver-kidney transplantation and to investigate the mechanism of action.DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed in the Medical College of Sun Yat-sen University between June 2004 and February 2006.MATERIALS: Male DA donor rats (n=60) and Lewis recipient rats (n=60), both were adult and of clean grade, were included.Sixty Lewis rats were randomly and evenly divided into 3 groups: IL-10-modified cell group, simple cell group, and model control group.METHODS: Donor rat liver and kidney were harvested by simultaneous liver-kidney transplantation. Recipient rats in each group were subjected to orthotopic liver and left kidney transplantation to establish models of immunological rejection. Under sterile condition, donor rat femur and tibia were harvested. Dnlbecco's modified eagle's medium (DMEM) was used to wash out the bone marrow. After removal of red cells, dendritic cells were isolated and cultured by adherent method. After modified with 20 μg/L IL-10 for 72 hours, dendritic cells were intravenously transfused into rat bodies in the IL-10-modified group, 2×10(7) cells/rat, In the simple cell group, rats were treated with donor dendritic cells without modification with IL-10. Rats in the model control group received no any interventions.MAIN OUTCOME MEASURES: [1]Dynamic changes of vital sign, urine volume, liver and renal function in recipient tissues;[2] Pathohistological detection results;[3]Distribution of donor dendritic cells in the recipient rats by in situ hybridization.RESULTS: In the simple cell and model control groups, urinary volume was reduced to 0 mL 5-6 days after transplantation. In addition, both groups presented with severe acute rejection. In the IL-10-modified cell group, urinary volume maintained at 6-12mL within 2 weeks after transplantation. The acute rejection of liver and kidney transplantation was obviously inhibited, surviving for(20.0±2.6) days on average, which was significantly longer than that in the simple cell group and model control group. A probability value of less than 0.05 in the Log Rank test was considered statistically significant. There were many Y chromosome-labeled dendritic cells immigrated into the mesenteric lymph node in the recipient rats.CONCLUSION: IL-10-modified dendritic cells play an immunoprotective effect on the liver and kidney transplanted simultaneously. Donor immature dendritic cells immigrated into recipient tissue could reduce acute rejections and prolong the survival time of liver and kidney grafts and recipients.

Objective To observe the biochemical indicators and lifestyles of patients with non-alcoholic fatty liver disease (NAFLD),and analyse the risk factors of induced NAFLD.Methods A total of 258 in-and out-patients of NAFLD were included in the present study,the control group consisted of 213 examinationers with nonfatty liver disease.All samples were being blood biochemical indicator detection and lifestyle survey.Results Some blood biochemical index of NAFLD patients such as TC(5.48 ± 1.10) mmol/L,TG(2.31 ± 1.25) mmol/L,ALT (51.35 ± 26.18) U/L,AST (42.37 ± 28.32) U/L,FPG (5.62 ± 3.24) mmol/L,GGT (58.47 ± 43.25) U/L and UA (398.51 ± 96.85) μmol/L were higher than those of the control group (t =3.423,5.250,7.402,4.348,3.326,6.683,3.891,all P ＜ 0.01) ; and the incidence of hyperlipidemia,hypertension (21.71％),diabetes (22.09％),BMI(26.85 ± 3.45) or metabolic syndrome (44.57％) etc.Those indexes in NAFLD patients were significantly higher than those in the control group (x2 =8.14,10.55,58.48,t =10.73,all P ＜ 0.01).By multivariable Logistic regression analysis,TG,WI,BMI,HOMA-IR,lack of exercise,high-fat diet were independent risk factors for NAFLD (all P ＜ 0.05).Conclusion Patients with NAFLD is closely correlated to metabolic abnormalities,reasonable diet and a healthy lifestyle is an effective way of prevention and treatment of this disease.

OBJECTIVETo explore the dose-length product calibration method for pencil ionization chamber in cobalt beams.

METHODSThe PTW TM30009 ionization chamber was placed into the central hole of T40017 head phantom and irradiated 60 s in 20 cm x 20 cm cobalt beam. The charge was collected with UNIDOS electrometer. Absorbed doses were measured with TM30013 0.6 mL farmer-type chamber under the same condition. The CT chamber calibration factor was expressed in dose-length product. Dose linearity and spatial response were also investigated.

RESULTSThe calibration factor in dose-length product was derived from measured data. Dose linearity and spatial response were good in cobalt beams.

CONCLUSIONIt is feasible to calibrate the CT chamber in cobalt beams for patient dose evaluation in MVCT.

Objective: To explore the symptomatic level of personality disorders,family environment of college students, and the correlation between these two variables. Methods: PDQ-4+(Personality Diagnostic Questionnaire-4+) and AAQ3.1 were administrated to 320 college students by layered sample. Results: Significant correlation was found between several factors of personality disorders and family environment. The personality disorders group get significantly lower score in Intimacy factor and higher score in Conflicts factor of family environment than the normal group. Conclusion: There was significant correlation between symptomatic level of personality disorders and family environment.

interface screw,with significant difference(P0.05).[Conclusion]All internal fixations in this study could meet biomechanical requirement,but Rigidfix and Intrafix showed more advantages.

Objective Experimentally to investigate the changes in vasospastic canine basilar arteries. Methods Vasospasm of basilar arteries was induced by double-injecting autologous blood into the brain cisterns. 28 adult mongrel dogs of both genders served as the subjects, of them 20 involved in experimental group and accepted the PTA treatment and the other 8 doges were in negative control group without any treatment. PTA was performed by a single inflation of balloon for 10 seconds at a peak pressure of 2.0 atmospheres. Angiography was re-performed at 30 day, 90 day and 300 day following PTA, respectively. The histologic changes were then evaluated using light and electron microscopy. Results Caliber of basilar arteries of all canines decompressed obviously, from 1.3 mm to 0.8 mm. In test group, PTA succeeded in 15 ones, with caliber of vasospastic basilar arteries enlarged obviously after PTA ( P

Objective To explore the application of non-breathholding photography technology in angiography. Methods 183 cases with abdominal and thoracic DSA examination were separated into non-breathholding experimental group and conventional breath-hold control group in proper order. The experimental group collected images in natural breathing and the conventional group gained images after inspiratory breathholding in imaging process. The images of two groups were adjusted by the post-processing functions and were print film; the quality of the images was evaluated. Results In the chest experimental group(n =37) had 36cases(97.30% )satisfactory and 1 case(2.70% ) unsatisfactory, and the control group(n=36)had 32cases(88.89%) satisfactory and 4 (11.11%) unsatisfactory. In the abdominal experimental group(n=55) had 53 cases(96.36%) satisfactory and 2 cases(3.64%) unsatisfactory images, and the control group(n=55) had 49 cases(89.09%) satisfactory and 6cases(10.91%) unsatisfactory images. There is no significant difference of the image quality between the two groups in each part (chest part ?2=2.02, 0.1

This paper introduces the Vest-style First-aid Kit which are newly developed for PLA.Adopting a vest structure the Vest-style First-aid Kit has the advantages of fully freeing both hands,and being fit for the ship confined space.Basic performance experiments,ergonomics tests and army tryout were performed and the results showed that the vest-style first-aid kit was scientifically designed,and easy to use,suiting the body,obviously saving physical energy.It has a reasonably designed interior medicine layout and can fulfill the requirements of first-aid operations in naval ships.

Objective To investigate the high risk factors of persistent pulmonary hypertension (PPHN) in preterm and full-term neonates.Methods Fifty-six newborns with PPHN were divided into preterm neonates group and the full-term neonates group according to their gestational ages.The relationship between PPHN and high risk factors such as the primary diseases,mother's complications during pregnancy,and the prognosis were analyzed retrospectively in each group.A retrospective review of lung samples of 3 neonates who died of respiratory failure were carried out.Results Thirty-one (55.36％) out of 56 neonates with PPHN including 27 preterm and 29 full-term newborns had parenchyma diseases.The first and the second primary diseases were meconium aspiration syndrome (MAS,9/29 cases,31.03％) and congenital heart diseases (8/29 cases,27.59％) in the full-term group,and respiratory distress syndrome (RDS,16/27 cases,59.26％) and infection (8/27 cases,29.63％) in preterm group,respectively.There were 15 cases (55.56％) whose mothers had complications during pregnancy among the 27 preterm neonates,and 8 ca ses (27.59％) among the 29 full-term neonates.The morbidity of mothers' complications during pregnancy in preterm neonates was higher than that in full-term neonates (x2 =4.519,P =0.034).The mortality rate was 33.33％ (9/27cases) in the preterm neonates group while 27.59％ (8/29 cases) in the full-term neonates group.There was no significant difference in mortality rate between each group (x2 =0.218,P =0.640).The primary diseases were RDS (9/9cases,100％) and MAS (4/8 cases,50％) in preterm and full-term neonates who died of PPHN,respectively.One case of pulmonary hypertensive vascular remodeling,1 case of alveolar capillary dysplasia (ACD) and 1 case of interstitial pneumonia were found in 3 lung samples of neonates died of PPHN.Conclusions The ratio of preterm increased in neonates with PPHN.RDS and infection were the first two primary diseases in preterm neonates with PPHN.MAS was the first primary disease in full-term neonates with PPHN.Primary pulmonary hypertension should be considered for neonates with severe PPHN.

The experiment was conducted to examine the effect of zinc toxicity on the peripheral blood T-lymphocyte by the method of flow cytometry(FCM) and ANAE.200 one-day-old Avian broilers were divided into four groups,and fed with the diets as follows:①control group(Zn 100 mg/kg diet) and ② zinc toxic groups(Zn 1 500 mg/kg diet,zinc toxic group Ⅰ;Zn 2 000 mg/kg diet,zinc toxic group Ⅱ;Zn 2 500 mg/kg diet,zinc toxic group Ⅲ) for seven weeks.The ANAE positive ratios of the peripheral blood T-lymphocytes were much lower in the three zinc toxic groups than that in control group at 7 weeks of age.CD4+ T cell numbers were reduced from 2 to 7 weekds of age in zinc toxic group Ⅲ and from 6 to 7 weeks of age in zinc toxic group Ⅱ,as compared with that of control group.The numbers of CD8+ T cell decreased at 2 and 4 weeks of age in zinc toxic groups Ⅱ and Ⅲ,and at 7 weeks of age in zinc grups Ⅰ and Ⅲ.CD4+/CD8+ ratio was higher at 2 and 4 weeks age,and lower at 6 and 7 weeks of age in zinc toxic groups Ⅰ,Ⅱ and Ⅲ than in control group.The results showed that zinc toxicity would suppress the development of T-lymphocytes and reduce the peripheral blood T-lymphocyte populations.Potential mechanism underlying these observations are also discussed.