AIM: To observe the pathological role of 3-nitrotynosine (3-NT) on Escherichia coli LPS-induced vascular hyporeactivity in rats and the therapeutic effect of antioxidants. METHODS: Forty male SD rats weighting from 200 g to 250 g were randomly divided into four groups: the control group (n=10); LPS shock group (n=10); uric acid-treated group (n=10); melatonin-treated group (n=10). 6 h after LPS shock, phenylephrine (0.5-2.5 ?g?kg -1) was applied intravenously to all groups and the percentage increase in MAP was detected, respectively. The concentration-response curve of aorta rings from all groups rats were obtained by cumulative addition of phenylephrine (PE), and PE E_ max, EC_ 50 were calculated. The concentrations of plasma malondialdehyde (MDA), nitrate/nitrite and 3-NT were assayed in all groups 6 h after LPS shock. RESULTS: The MAP level induced by PE significantly decreased to 54.60% in LPS shock rats compared with the control (P

The concepts of Western medicine in treating tumor have changed markedly in recent years, and begun to put more emphasis on the whole survival status of patients. With recent emergence of targeted drugs, it was found that curative effects of the same targeted drugs in treating patients with the same tumor type even the same tumor pathological subtype but different therapeutic targets may be different. On the contrary, the curative effects of different targeted drugs in treating patients with different tumor types but the same therapeutic targets may be similar. Accordingly, the viewpoint of personalized therapy is advocated in Western medicine. Under the guidance of syndrome differentiation treatment in traditional Chinese medicine (TCM), patients with the same tumor and different syndromes should be treated by different herbs, and patients with different tumor and the same syndrome should be treated by the same herbs. Both the theories of "the same treatment for the same syndrome" and "the same treatment for the same therapeutic target" indicate that personalized therapy in Western medicine is similar to TCM syndrome differentiation treatment. The article focuses on "different treatments for the same disease" as well as "the same treatment for different diseases" to describe the similar points between personalized therapy and TCM syndrome differentiation treatment and thereby proves that TCM syndrome differentiation treatment is a scientific and essential approach in treating tumor. At the same time, the article also points out the problems and solution methods of TCM syndrome differentiation treatment.

It is of essential importance for college students to think creatively. Flexible teaching methods have been adopted during the teaching of medical writing in our university. We inspired, guided, stimulated students to develop their creative thought. The purpose of the article is to develop the creative thought of the students in medical universities.

Objective We aimed to develop a safe, comfortable and effective perineal washer, which prevent perineum wound from infection and improve the healing of perineal wound. Methods The perineal washer was made from high polymer medical material, including two metal catheters, pressing gasbag, medical yoke and dicty blow head. We divided women patients after vagina delivery with perineum wound or after caesarean section into the observation group and the control group randomly with 120 cases in each group from Janurary to October 2007. The control group was given perineal washing by routine washing pot and cotton ball with forceps. The observation group received perineal washing by perineal washer with cleaning fluid and was scrubbed by sponge brash. The elimination of wood swelling, the healing of perineum healing, the urinary infection for patients with indwelling urinary catheterization and patients comfort degree were observed in the two groups. Results Statistical difference existed between the two groups in such aspects, elimination of wound swelling wound healing urinary tract infection and patients comfort degree, P<0.05 or P<0.01. Conclusions Adoption of self designed perineal washer accompanied with sponge brush could completely delete bacteria and inflammatory secretion deep in the vagina and prevent the incidence of infection. It not only could hasten the elimination of wound swelling, alleviate the pain and wound healing, but also was easily developed and portable to use. It provided a new and effective method for perineal washing and is worth clinical application.

Objective To investigate the effective nursing measures of reducing the complications of intratra-cheal suction about mechanical ventilation patients to improve airway nursing quality. Methods 80 patients with ar-tificial air passage mechanical ventilation were divided into Control group (Group one, 40 cases) and Testing group (Group two, 40 cases) randomly. In the control group, suck at regular time after injecting normal saline solution in-to the in-airway (in order to diluted the sputum). There are improvement that nursing measures in testing group. For sample, suck sputums according to demands, choose appropriate sucking sputums tube, control the deepth of sucking sputum tube, adjust the negative pressure based on the deepth of sucking sputum tube, give pure oxygen two to three minutes before and after the suction, strengthen the airway humidfication, lubricate the sucking sputum tube with chlorotetracycline eye ointment, etc. Complications of intratracheal suction were compared in two groups. Results The comparison showed that the complications have a significant statistical difference in sputum obstruction, SpO2 de-scending to 80 percent and below during suction and airway mucous membrane traumatic hemorrhage. (P < 0.01). Conclusion The improved intratracheal suction will decrease the complications obviously like sputum obstruction, SpO2 reducing to 80 percent and below during suction and airway mucous membrane traumatic hemorrhage.

Idiopathic orbital inflammatory pseudotumor (IOIP) is a common ophthalmic disease that severely damages the visual acuity and countenance of the patient,its etiology and pathogenesis are unclear.Many hypotheses have been proposed,including infection,autoimmunity and neuroendocrine mechanism,etc..These hypotheses reflect certain aspects of the etiology and pathogenesis of IOIP and provide information for further understanding of the disease.The research progression in the etiology and pathogenesis of IOIP was reviewed.

In recent years,immunoglobulin G4-related disease (IgG4-related disease) has received increasingly attention in medical community as a novel clinical entity.Although its clinical manifestation varies,high serum IgG4 concentration and numerous IgG4-positive plasma c(c)ll infiltration are the common characteristics.IgG4-related disease involves many human organs,and most one is pancreas,followed by parotid gland,bile duct,liver,lung,lymph node and so on,and orbital involvement is relatively rare.Current initial research shows that there is certain association between orbital disease with IgG4,and these diseases mainly include benign lymphoepithelial lesion,idiopathic orbital inflammatory pseudotumor,Castleman ' s disease,xanthogranuloma,Rosai-Dorfman disease etc..The researching progression in the relationship between IgG4 and orbital diseases is reviewed.

Idiopathic orbital inflammatory pseudotumor (IOIP) is a common ophthalmic disease with uncertain etiology and pathogenesis.It charactarized by chronic inflammatory cell infiltration and connective tissue proliferation.At present,its clinical treatments mainly include pharmacotherapy,radiation therapy and surgery,and the primary treating drugs include glucocorticosteroids and immunosuppressive agents.The mainstay of therapy is systemic glucocorticosteroids,although there is an increasing trend toward the use of alkylating agents,antimetabolites,monoclonal antibodies and other immunosuppressive agents.The progress in clinical treatment of IOIP was reviewed.

Objective:To study the biological functions of bone marrow macrophage (BMM ?) and to establish the methodology of separation, purification, cultivation and identification of BMM ?.Methods:Using the techniques of anchorage-dependent culture of separated rat bone marrow cell (rBMC) in DMEM culture media (contain 20% horse serum,20% (v/v)L 929 conditioned media) in vitro,a lot of purified anchor cells were obtained, and these cells were identified with specifically biological marker of macrophage, such as 1, morphologicalobservation:invert phase contrast microscopy, light and electron microscopy;2, enzyme cytochemistry:acid phosphatase (ACP),?-acetic acid naphthol esterase (?-ANE);3,phagocytic experiment:phagocytosis of chicken erythrocytes and prepared Chinese ink;4,immunocytochemistry: surface specific antigen of macrophage ( CD 68 stain).Results:The cells were purified having functional satisfactory macrophage according to morphological observation,enzyme cytochemistry, phagocytic experiment and immunocytochemistry.Conclusion:This is a simple and easy method for separation, purification, cultivation and identification of rat marrow macrophage.

Colorectal cancer (CRC) generally occurs in older patients, however the proportion of young patients with CRC is increasing in recent years, which has drawn lots of scholar′s attention, but their research results were controversial. The current literature from several fields, including clinicopathological characteristic of young CRC patients, the optimal age cutoff between young and elder CRC patients, and the prognosis, early screening and diagnosis in young CRC patients will be reviewed.